Objective To study the learning and memory abilities together with the morphological changes in astrocyte and neuron in the hippocampal CA3 area in mice induced by chronic cadmium exposure. Methods Twenty Kunming mice aged 4-5 months were selected by Y-maze and randomly divided into two groups. The cadmium exposed group treated with cadmium (CdCl2,2 mg/kg) by subcutaneous injection,twice a week for 3 consecutive months,and the normal control group were injected with the equal dose of saline. The learning and memory abilities were detected by Y-maze after 3 months of treatment. The structure of astrocytes and neurons in CA3 area of hippocampus were observed under light microscope,and the quantitatively analysis was performed by cell morphometric technique. Results Compared with the control group,learning and memory capacity determined by Y-maze test in the cadmium exposed group were lower (P

Objective To explore the appropriate method of isolating,purifying and culturing the rat bone marrow mesenchymal stem cells(MSCs) in vitro,and observe the differentiation of MSCs into neuron-like cells induced by noggin gene transfected with adenovirus vector.Methods MSCs obtained from bone marrow of SD rats were isolated,cultivated and amplified by Percoll density gradient centrifugation with adherent method,the the third passage of purified MSCs was then induced to differentiate into neurocytes by using the reconstructed noggin adenovirus vector(pAdEasy-1-GFP-noggin) and control vector(pAdEasy-1-GFP),respectively.After cultivation,the differentiated cells were identified by using immunocytochemical method with neurone-specific enolase(NSE),neurofilament 200(NF-200),neuronal nuclei(NeuN) and glial fibrillary acidic protein(GFAP),and the inductivity in the respective groups were analyzed.The Nissl bodies in the induced cells were displayed by thionine-eosin staining.Results The primarily cultured MSCs were spindle in shape,adhered 24 hours after cultivation,and then grew into small clones.Forty-eight hours after transfection by noggin recombinant adenovirus vector,the MSCs started to change their shape as observed under inverted microscope,and several axon-or dendrite-like processes with branches stretched out from the cell body.The induced cells derived from bone marrow MSCs specifically expressed NSE,NF and NeuN,but not GFAP by immunocytochemistry.A lot of Nissl bodies could be seen in the cell body of induced cells shown by Nissl staining.Conclusions Highly purified MSCs can be obtained by Percoll density gradient centrifugation combined with adherent method.The bone marrow derived MSCs transfected with noggin gene can differentiate into neuron-like cells in vitro.

Objective: To provide scientific evidence for the prevention and control of the hand, foot and mouth disease (HFMD), we analyzed the epidemiological characteristics and pathogen surveillance of HFMD cases which were reported in Liaoning Province from 2008 to 2017. Methods: The information of cases and laboratory monitoring were collected from the HFMD reports of 2008-2017. The epidemic intensity, three distribution and pathogen composition of HFMD were analyzed by using descriptive epidemiological method . Results: There were 290 339 cases reported during 2008 to 2017 and the average incidence was 66.56/100000. The peak incidence was found for June to August, The incidence of crowd was mainly in the 0-6 years old children, and the male / female incidence ratio was 1.50∶1. Among 16 498 laboratory diagnosis cases, EV-A71 positive cases were 4 974 (30.15%), CV-A16 positive cases were 5 130(31.10%), and the other enteroviruses positive cases were 6 394 (38.76%). Conclusions The overall situation of HFMD in Liaoning province showed a fluctuating downward trend from 2008 to 2017. The epidemic had obvious seasonality and population, and the pathogenic characteristics were constantly changing, Monitoring should be strengthened.

Background Natural product sanguinarine chloride (SC) can significantly alleviate liver fibrosis and acute liver injury in mice, but whether it has a protective effect on mouse liver injury caused by sodium arsenite (SA) has not been studied. Objective To verify if SC may present preventive and therapeutic effects on SA-induced liver injury in mice. Methods A total of 140 SPF male Kunming mice were randomly divided into two sub-studies, which included a prevention sub-study and a treatment sub-study. In each sub-study, a blank group (normal saline), a model group (5 mg·kg⁻¹ SA), and a positive control group (11.375 mg·kg⁻¹ bicyclol and 182 mg·kg⁻¹ glutathione), as well as SC low, medium, and high dose groups (25, 50, and 100 mg·kg⁻¹) were arranged with 10 mice in each group. In the prevention sub-study, the blank group was given normal saline, the model group was given SA, and the other groups (the SC low, medium, and high dose groups and the positive control group) were given the corresponding treatment 30 min before gavage of SA, once a day, for 28 d. In the treatment sub-study, except for the blank group which was given normal saline, the other groups were given SA for 28 d, then the model group was given normal saline, and the other groups were given the corresponding treatment every day for 28 d. After the experiment, the mice were sacrificed to evaluate selected physiological and biochemical indicators in serum and liver tissue and to observe histopathological changes after HE staining. Results In either sub-study of preventive effect or treatment effect: compared with the blank group, body weight, liver weight, liver coefficient, as well as serum alanine transaminase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), malondialdehyde (MDA), glutathione peroxidase (GSH), and superoxide dismutase (SOD) among all SC groups were not significantly different (P>0.05); but compared with the model group, the SC groups showed increased body weight (P<0.01), decreased liver weight and liver coefficient (P<0.01), reduced ALT, AST, TBIL, and MDA (P<0.05 or P<0.01), and increased GSH and SOD with (P<0.05 or P<0.01) or without significance; compared with the positive control group, no differences were found in the above indicators (P＞0.05). The result of histopathological evaluation showed that the SC groups had a clear liver lobule structure, neatly arranged hepatic cords, and less infiltration of inflammatory cells. Conclusion SC has both preventive and therapeutic effects on SA-induced liver injury in mice.

Objective:To study and verify the molecular mechanism of Duzhong Pills for osteoporosis (OP) by means of network pharmacology and molecular docking.Methods:The main chemical components of Duzhong Pills were mined by TCMSP database and the related targets were predicted. The potential targets of osteoporosis in GeneCards, DisGeNET and OMIM databases were searched and the common targets of both were obtained. The STRING platform was used for protein interaction analysis and PPI network diagram was made. The common targets were imported into the David database for enrichment analysis of GO and KEGG pathways, and molecular docking of the main components and core targets was performed. Eighteen Sprague-Dawley rats were divided into control group, model group and Duzhong Pills group according to random number table method, with 6 rats in each group. Ovariectomy was used to make osteoporosis model in model group and Duzhong Pills group. Duzhong Pills group was intragaxed with Duzhong Pills extract of 5 g/kg, and control group and model group were intragaxed with normal saline of the same volume, once a day for 8 weeks. Serum levels of TNF-α, IL-6 and IL-1β were detected by ELISA, and femur PI3K and Akt were detected by Western blot.Results:34 active components were obtained from Duzhong Pills, corresponding to 243 targets, and 1 059 targets for osteoporosis. The core targets included TNF-α, IL-6, AKT1, TP53, IL-1β and others regulated osteoporosis through PI3K-Akt and TNF pathway. The experimental results indicated that compared with model group, the levels of serum TNF-α, IL-6 and IL-1β in Duzhong Pills group decreased ( P<0.05), and the expressions of PI3K and Akt in femur decreased ( P<0.05). Conclusion:Through β-sitosterol, quercetin, kaempferol and other active components, Duzhong Pills can act on TNF, IL-6, AKT1, TP53, IL-1β and other targets, regulating PI3K-Akt signaling pathway, TNF signaling pathway and other signaling pathways to play a role in the treatment of osteoporosis.

The COVID-19 epidemic has spread to the whole world for three years and has had a serious impact on human life, health and economic activities. China's epidemic prevention and control has gone through the following stages: emergency unconventional stage, emergency normalization stage, and the transitional stage from the emergency normalization to the "Category B infectious disease treated as Category B" normalization, and achieved a major and decisive victory. The designated hospitals for prevention and control of COVID-19 epidemic in Tianjin has successfully completed its tasks in all stages of epidemic prevention and control, and has accumulated valuable experience. This article summarizes the experience of constructing a hospital infection prevention and control system during the "Category B infectious disease treated as Category A" period in designated hospital. The experience is summarized as the "Cluster" hospital infection prevention and control system, namely "three rings" outside, middle and inside, "three districts" of green, orange and red, "three things" before, during and after the event, "two-day pre-purification" and "two-director system", and "one zone" management. In emergency situations, we adopt a simplified version of the cluster hospital infection prevention and control system. In emergency situations, a simplified version of the "Cluster" hospital infection prevention and control system can be adopted. This system has the following characteristics: firstly, the system emphasizes the characteristics of "cluster" and the overall management of key measures to avoid any shortcomings. The second, it emphasizes the transformation of infection control concepts to maximize the safety of medical services through infection control. The third, it emphasizes the optimization of the process. The prevention and control measures should be comprehensive and focused, while also preventing excessive use. The measures emphasize the use of the least resources to achieve the best infection control effect. The fourth, it emphasizes the quality control work of infection control, pays attention to the importance of the process, and advocates the concept of "system slimming, process fattening". Fifthly, it emphasizes that the future development depends on artificial intelligence, in order to improve the quality and efficiency of prevention and control to the greatest extent. Sixth, hospitals need to strengthen continuous training and retraining. We utilize diverse training methods, including artificial intelligence, to ensure that infection control policies and procedures are simple. We have established an evaluation and feedback mechanism to ensure that medical personnel are in an emergency state at all times.