Objective To investigate Notch signaling pathway expression and its relationship with clinicopathological parameters in human gastric cancer.Methods Specimens of carcinoma tissues and nodal gastric membrane were obtained from 63 gastric cancer patients undergoing radical surgery from Jan 1999 to Dec 2001.Notehl,Deltal,Jaggedl and NFKB protein were measured using fissure microarray and immunohistochemistry.Data were analysed using SPSS software.Results Notchl,Deltal,Jaggedl and NFKB protein were upregulated in gastric cancer tissue compared with adjacent normal tissue(P＜0.01).The expression of Notchl protein in the well--differentiated tumors was higher than that in the poordifferentiated tumors(P＜0.05).Positive linear correlation was established between the expression of Jaggedl and NFkB.Conclusion Notchl signal transduction pathway plays an important role in the development of human gastric cancer by activation of NFKB through Notchl-Jaggedl signal transduction pathway.It is also evident that Notch signaling can be both oncogenic and tumor suppressive in human gastric cancer.

Here we reported a research project based on Black-board to integrate medical curriculum .The key points of this research is application of clinical cases as teaching data and facilitate learning of knowledge following the principle of learning by doing and , input the concept of precision medicine and informatics in learning process with an individually designed framework of learning .The learning outcome is evaluated with big data tech-nology and thus creates a student-centered pathway of medical education .