Objective To investigate the clinical effect of hyperbaric oxygen combined with Xianling Gubao capsule in delayed union of elderly proximal femoral fractures.Methods 64 elder patients with proximal femoral fracture form May 2013 to December 2015 in our hospital were selected and divided into two groups.32 cases in the control group were treated with routine clinical treatment, and 32 cases in the experimental group were treated on the basis of the control group with Xianling Gubao capsule combined with hyperbaric oxygen.Fracture healing time and clinical efficacy were compared after the treatment.Results The fracture healing time of experimental group was shorter than that in control group ( P<0.05 ) .The pain degree in experimental group was lower than that in control group ( P<0.05 ) .The efficacy in experimental group was higher than that in control group ( Z =-2.144,P<0.05).There was no significant difference in total adverse reactions between experimental group and control group (χ2 =0.474, P>0.05).Conclusion Xianling Gubao capsule combined with hyperbaric oxygen in the treatment of delayed union of proximal femoral fractures in elderly patients has better clinical curative effect and high safety.

To investigate the risk factors for intracerebral hemorrhage in general population.Methods:The related research was searched through English Medical Current Contents (EMCC),China Hospital Knowledge Database (CHKD),MEDLINE,and Chinese Biomedical Literature Database (CBM).The search terms were intracerebral hemorrhage,factor,and case-control study or cohort study.Results:There were 8 literatures with original data were in accordance with the inclusion criteria.All the data could not be combined because there were some differences in counting and metrology in the risk factors included in all the studies.Hypertension,family history of cerebrovascular disease,high salt diet,alcohol consumption,diabetes mellitus,high diastolic pressure,high systolic pressure,smoking,snoring disease,and increased weighted mean difference of body mass index (BMI) (95% confidence interval) were 5.71 (4.00-6.79),3.54 (2.44-5.14),2.58 (1.94-3.43),2.80 (2.29-3.43),2.78 (1.83-4.23,1.90 (1.35-2.70),17.76 (16.60-18.92),30.43 (28.61-32.25),5.42 (5.15-5.70),1.90 (1.34-2.69),6.88 (4.61-10.26,and 5.42 (5.15-5.70),respectively.There were significant differences between the patient groups and control groups among the above indexes (all P<0.000 01).Conclusions:The risk factors for intracerebral hemorrhage include hypertension,family history of cerebrovascular disease,high salt diet,smoking,alcohol consumption,snoring disease,diabetes mellitus,overweight,high diastolic blood pressure,high systolic blood pressure and increased BMI.

BACKGROUND: Bone morphogenetic protein in combination with hol ow porous titanium al oy can improve the affinity with surrounding bone tissues. OBJECTIVE: To observe the effects of bone morphogenetic protein 2 on growth and osteogenic differentiation of bone marrow mesenchymal stem cel s cuftured on a hol ow porous metal prosthesis scaffold. METHODS: Passage 3 Sprague-Dawley rat bone marrow mesenchymal stem cel s were directly inoculated onto a hol ow porous metal prosthesis, and then the scaffold was cultured in DMEM medium containing 0, 0.001, 0.01, 0.06 and 0.1 g/L bone morphogenetic protein 2, respectively. At 6, 12, 24 and 48 hours after inoculation, cel adhesion was detected by MTT assay. Cel osteogenic differentiation was detected by alizarin red staining at 18 days. Besides, Transwel culture was put on the scaffold, and 5x108/L bone marrow mesenchymal stem cel s were added into the upper chamber, and DMEM medium containing 0, 0.001, 0.01, 0.06 and 0.1 g/L bone morphogenetic protein 2 were added into the lower chamber to observe cel migration capability after 0, 6, 12, 24 and 48 hours culture. RESULTS AND CONCLUSION: After 6-48 hours of inoculation, different mass concentrations of bone morphogenetic protein 2 promoted adhesion of bone marrow mesenchymal stem cells in a time-dependent manner. After 18 days of inoculation, bone marrow mesenchymal stem cells induced by different mass concentrations of bone morphogenetic protein 2 changed from fusiform to polygon, and arranged in a multilayer and overlapped form. Numerous calcified nodules could be found, which were stained red by alizarin red. Additionally, within 6-48 hours of culture, bone morphogenetic protein 2 could promote the migration of bone marrow mesenchymal stem cells in a concentration-and time-dependent manner. In conclusion, bone morphogenetic protein 2 can enhance the adhesion, osteogenic differentiation and migration of bone marrow mesenchymal stem cells cultured on the hollow porous metal prosthesis.

Objective To investigate the Camtrylobacterjejuni's risk factors which were associated with the development of Guillain-Barre syndrome( GBS), the galE gene of C. jejuni strains were sequenced and the sequencing results were compared with other C. jejuni strains. Methods Selecting three GBS-asso-ciated C.jejuni strains isolated from stools of GBS patients who had been diagnosed as AMAN pattern by clin-ical and electrophysiological test from Hebei province, China. After sequencing galE gene, the results were spliced and assembled into a complete sequence by the terminals overlapped each other. The sequences of galE gene were compared with the corresponding sequences in GenBank to find the mutation and constructed the phylogenetic tree. Results The variation frequency of galE sequences of GBS-associated C. jejuni were higher than that of non-GBS-associated C. jejuni. The phylogenetic analysis demonstrated that each of the three C. jejuni strains was separately genetically closed to three strains which sequences have published in GenBank. The alignment with the related sequence of NCTC11168 shows that there are 4 same mutations in the galE gene of the three C. jejuni strains. The phylogenic tree reflected the regional feature of C. jejuni. Conclusion The probability of sequence variation of galE of GBS-associated C.jejuni is significantly higher than non-GBS-associated C. jejuni strains, the relation between the variation and GBS-pathogenesis remains to be further confirmed. The mutations found in the three C. jejuni strains established the foundation for ex-ploring the biologically characteristic of GBS-associated C. jejuni strains.

Objective To analyze medication rules of traditional Chinese medicine in the treat-ment of thyroid nodules based on real-world data mining technology.Methods Chinese medicine prescriptions for patients with thyroid nodules as clinical first diagnosis in the Department of Thyroid and Breast Surgery in Qingdao Hospital of Traditional Chinese Medicine from August 2018 to August 2023 were collected,and the Traditional Chinese Medicine Inheritance Assistant Platform(V3.0)was used to analyze the medication rules.Results A total of 1 206 traditional Chinese medicine pre-scriptions for the treatment of thyroid nodules were screened,involving 291 traditional Chinese medi-cines;the top five most frequently used drugs were Fritillaria thunbergii,Prunella vulgaris,Bupleu-rum chinense,Poria cocos,and Pinellia ternata;the main function of drugs were clearing heat,toni-fying deficiency,resolving phlegm,activating blood circulation,and regulating qi;the main property of the drugs was cold,the main tastes of drugs were bitter,sweet and spicy,and the meridians in-volved were mainly the lung,liver and spleen meridians;association rule analysis identified 17 high-frequency drug combinations,with Fritillaria thunbergii-Prunella vulgaris as the most frequently oc-curring drug pair;among the 16 association rules,Radix Scrophulariae-Fritillaria thunbergii and Rhi-zoma Cyperi-Bupleurum chinense had the highest confidence level;clustering analysis found 6 core drug combinations.Conclusion The main idea of clinical treatment of thyroid nodules in traditional Chinese medicine is to resolve phlegm and reduce nodules,promote qi circulation and blood circulation,and nourish qi and blood;the common herbal combination of Fritillaria thunbergii and Prunella vul-garis is used to clear heat,resolve phlegm,and reduce nodules,and the basic formula for resolving scrofula is based on Xiaoluo Pills,which resolves phlegm,softens hardened nodules,and reduces nodules.

Objective To analyze medication rules of traditional Chinese medicine in the treat-ment of thyroid nodules based on real-world data mining technology.Methods Chinese medicine prescriptions for patients with thyroid nodules as clinical first diagnosis in the Department of Thyroid and Breast Surgery in Qingdao Hospital of Traditional Chinese Medicine from August 2018 to August 2023 were collected,and the Traditional Chinese Medicine Inheritance Assistant Platform(V3.0)was used to analyze the medication rules.Results A total of 1 206 traditional Chinese medicine pre-scriptions for the treatment of thyroid nodules were screened,involving 291 traditional Chinese medi-cines;the top five most frequently used drugs were Fritillaria thunbergii,Prunella vulgaris,Bupleu-rum chinense,Poria cocos,and Pinellia ternata;the main function of drugs were clearing heat,toni-fying deficiency,resolving phlegm,activating blood circulation,and regulating qi;the main property of the drugs was cold,the main tastes of drugs were bitter,sweet and spicy,and the meridians in-volved were mainly the lung,liver and spleen meridians;association rule analysis identified 17 high-frequency drug combinations,with Fritillaria thunbergii-Prunella vulgaris as the most frequently oc-curring drug pair;among the 16 association rules,Radix Scrophulariae-Fritillaria thunbergii and Rhi-zoma Cyperi-Bupleurum chinense had the highest confidence level;clustering analysis found 6 core drug combinations.Conclusion The main idea of clinical treatment of thyroid nodules in traditional Chinese medicine is to resolve phlegm and reduce nodules,promote qi circulation and blood circulation,and nourish qi and blood;the common herbal combination of Fritillaria thunbergii and Prunella vul-garis is used to clear heat,resolve phlegm,and reduce nodules,and the basic formula for resolving scrofula is based on Xiaoluo Pills,which resolves phlegm,softens hardened nodules,and reduces nodules.

BACKGROUND:Transcranial magneto-acoustic-electrical stimulation is a novel non-invasive neural regulation technique that utilizes the induced electric field generated by the coupling effect of ultrasound and static magnetic field to regulate the discharge activity of the nervous system.However,the mechanism by which it affects synaptic plasticity in the brain is still not enough. OBJECTIVE:To explore the effect of transcranial magneto-acoustic-electrical stimulation intensity on synaptic plasticity of the prefrontal cortex neural network in mice. METHODS:(1)Animal experiment:Twenty-four C57 mice were equally and randomly divided into four groups:the control group receiving pseudo-stimulation,the 6.35 W/cm2 stimulation group receiving coupled stimulation of 0.3 T,6.35 W/cm2,the 17.36 W/cm2 stimulation group receiving coupled stimulation of 0.3 T,17.36 W/cm2,and the 56.25 W/cm2 stimulation group receiving coupled stimulation of 0.3 T,56.25 W/cm2.The local field potential signals and behavioral correctness were recorded during the execution of T-maze in mice.(2)Modeling and simulation experiments:A neural network model of the prefrontal cortex in mice stimulated by transcranial magneto-acoustic-electrical stimulation was constructed to compare the structural connectivity characteristics of the neural network under different stimulation intensities. RESULTS AND CONCLUSION:Transcranial magneto-acoustic-electrical stimulation could effectively shorten the behavior learning time,improve the working memory ability of mice(P＜0.05),and continue to stimulate the frontal lobe of mice after learning behavior.There was no significant difference in the accuracy of the T-maze behavioral experiment among the experimental groups(P＞0.1).Analysis of local field potential signals in the frontal lobe of mice revealed that transcranial magneto-acoustic-electrical stimulation promoted energy enhancement of β and γ rhythms.As the stimulation intensity increased,there was an asynchronous decrease in β and γ rhythms.Through β-γ phase amplitude coupling,it was found that stimuli could enhance the neural network's ability to adapt to new information and task requirements.Modeling and simulation experiments found that stimulation could enhance the discharge level of the neural network,increase the long-term synaptic weight level,and decrease the short-term synaptic weight level only when the stimulation intensity was high.To conclude,there is a complex nonlinear relationship between different stimulus intensities and the functional structure of neural networks.This neural regulation technique may provide new possibilities for the treatment of related neurological diseases such as synaptic dysfunction and neural network abnormalities.