Zoucheng was a high endemic area of bancrotian filariasis with Culex pipiens pallens as the principal transmitting vector. Transmissiom of the disease was interrupted in 1981. Epidemiological surveillance has been then carried out. After 1989, parasitological and immunological examinations were conducted for those cases previously with and without microfilaremia, entomological surveillance was also carried out. Results showed that the antibody level in inhabitants has been at normal level, and no infection has been found in mosquitoes.

OBJECTIVE:To detect curative mechanism of bushen huoxue huatan formula on polycystic ovarian syndrome (PCOS)model rats. METHODS:50 rats were randomly divided into normal control group,model control group,positive control group [rosiglitazone,3.0 mg/(kg·d)] and Bushen huoxue huatan formula high-dose,low-dose groups [7.5,5.0 g/(kg·d)],10 in each group. Except for normal control group,the PCOS model was induced by Poresky method in other groups. After modeling, normal control group and model control group were received normal saline intragastrically,medicine groups were received corre-sponding liquids intragastrically,once a day,for 22 d. After administration,the serum levels of testosterone (T),anti-mullerian hormone (AMH),insulin-like growth factor Ⅰ(IGF-Ⅰ),tumor necrosis factor-α(TNF-α) and the positive expression of trans-forming growth factor-β1(TGF-β1)in ovarian tissue were determined. RESULTS:Compared with normal control group,T,AMH, IGF-Ⅰ,TNF-αlevels in serum and TGF-β1 positive expression rate in ovarian tissue of rats in model control group were significant-ly increased(P<0.05);compared with model control group,T,AMH,IGF-Ⅰ,TNF-α levels in serum and TGF-β1 positive ex-pression rate of rats in ovarian tissue in other medicine groups were significantly decreased(P<0.05),and the indexes in Bushen huoxue huatan formula different doses groups were superior to positive control group(P<0.05). CONCLUSIONS:Bushen huoxue huatan formula can obviously reduce the T,AMH,IGF-Ⅰ,TNF-α levels in serum and TGF-β1 positive expression rate in ovarian tissue in model rats with PCOS,which may be one of the mechanisms of its treatment for PCOS.

OBJECTIVE:To explore the effects of Bushen huoxue huatan decoction on ovarian blood supply and form in model rats with polycystic ovarian syndrome(PCOS). METHODS:50 SD female rats were randomized into normal control group,model group,positive control group [rosiglitazone,3 mg/(kg·d)],Bushen huoxue huatan decoction low-dose and high-dose groups [5.0, 7.5 g/(kg·d)],with 10 rats in each group. Except for normal control group,PCOS model was induced in the other groups by Poresky method. After modeling,treatment groups were given relevant medicine intragastrically. The normal control group and mod-el group were given normal saline [10 mL/(kg·d)] intragastrically,once a day,for 22 days. After the end of administration,the rats were sacrificed. The microvessel density (MVD) was recorded. mRNA expression of vascular endothelial growth factor (VEGF), ovarian angiotensin Ⅱ receptor(Ang Ⅱ)type 1(AT1)and type 2(AT2),G-protein coupled receptor MAS were all detected in ovarian tissue of rats. The ovarian form was observed. RESULTS:Compared with normal control group,MVD of mod-el group and treatment groups were all increased,while mRNA expression of AT1,AT2 and MAS were down-regulated (P<0.05);cystic sinus sollicle,the decrease of granular cell layer,egg mother cells disappearance,the decrease of luteal tissue and other obvious pathological changes could be found in ovarian tissue of rats in model group. Compared with model group,above in-dexes of treatment groups were improved significantly,and the effects of Bushen huoxue huatan decoction low-dose and high-dose groups were better than that of positive control group (P<0.05). The pathological changes of ovarian tissue in treatment groups were all improved to certain extent. CONCLUSIONS:Bushen huoxue huatan decoction can improve ovarian blood supply,angio-genesis and ovulation in PCOS rats. It is beneficial to improve ovarian form.