3.Preliminary results of intraoperative radiation therapy for cervical carcinomaⅡb
Chinese Journal of Obstetrics and Gynecology 2000;0(09):-
0.05). However, there were quite improvements of survival rate and survival quality for cervical adenocacinoma. Their 5-year survival rates were 91% and 33% (P
6.A more accurate and efficient fluorescent probe of Caspase-8 activity based on flow cytometric fluorescence resonance energy transfer.
Acta Pharmaceutica Sinica 2015;50(3):291-297
As a classic fluorescent detect technique, fluorescence resonance energy transfer (FRET) has been widely used in biological researches. Researchers have developed a series of fluorescence detect probes which were based on FRET. Caspase family plays an important role in apoptosis pathway, especially Caspase-8 which located, at the initial of death receptor mediated apoptosis pathway, whose its activation can trigger subsequent precaspases' activation and lead to apoptosis. So it is of great significance to detect the activation of Caspase-8 in apoptosis assay. In this study, a fluorescent probe based on FRET has been designed which can detect the activity change of Caspase-8 in cells. To identify the effectiveness and specificity of the probe, we measure the Caspase-8 activity under the Caspase-8 specifically activated apoptosis inducer RGD-TRAIL with the flow cytometry FRET detection platform. The results show that the probe can respond to the activity change of Caspase-8 in apoptotic cells, and the change can be quantified rapidly by flow cytometry. The study provides a more efficient and convenient detection method of Caspase-8 activity in living cells.
Apoptosis
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Caspase 8
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metabolism
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Flow Cytometry
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Fluorescence Resonance Energy Transfer
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Fluorescent Dyes
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Humans
7.Study on the short version of the BFS mood scale and its reliability and validity
Jinjin GAO ; Fei ZI ; Yiwen CHEN
Chinese Journal of Behavioral Medicine and Brain Science 2012;21(4):373-375
ObjectiveTo provide a brief scale of mood measurement with satisfactory reliability and validity.MethodsThe 40-item BFS was abbreviated to a short-vcrsioncd BFS by item analysis.282 adult participants were surveyed by the brief BFS and NEO-FFI while NEO-FFI was taken as a criterion instrument.Reliability and validity of brief BFS were examined.ResultsThe brief BFS had 18 items including six dimensions ( activity,cheer,calmness,indignation,stir and depression),and the cronbach's α were 0.87,0.75,0.85,0.86,0.84 and 0.86 respectively and the retest reliability( r value) were 0.67,0.57,0.61,0.62,0.75 and 0.69 respectively.All the key fit indices of confirmatory factor analysis (CFA) of brief BFS were above 0.90.Besides,NEO-FFI had significant correlation with brief BFS.ConclusionThe brief BFS has satisfactory reliability and validity.
8.Correlation between integrated pharmacokinetics and pharmacodynamics of bile processed Rhizoma Coptidis in febrile rats.
Jing WANG ; Yue CHEN ; Zi-min YUAN
Acta Pharmaceutica Sinica 2016;51(1):127-131
This study was designed to validate the correlation between integrated pharmacokinetic and therapeutic effects of alkaloids using bile processed Rhizoma Coptidis (BRC). Rats were divided into three groups: normal, disease model, model+BRC. Rats were induced to have an excessive heat syndrome. Rectal temperatures were collected at 0, 3, 6 and 9 h after single oral administration of the drugs. The plasma concentrations of three alkaloids were quantified at different times by UPLC-MS/MS after the administration of BRC. An approach of self-defined weighting coefficiency was created to the holistic pharmacokinetic profiles of alkaloids in BRC. The classified and integrated synthetic concentrations were obtained, and then the pharmacokinetic parameters of alkaloids were calculated from non-compartmental model analysis. The potential relationship between the integrated mean concentration of alkaloids and the antifebrile efficacy was investigated. The holistic t(max) of alkaloids was 1.11 h, the antifebrile effect of BRC at 3 h was improved over the model group. Double peaking appeared in the integrated blood concentration-time curve, the second t(max) of alkaloids was 4.82 h. The antifebrile effects of BRC at 3-6 h were significant, and the antifebrile effects at 6-9 h was decreased significantly. Dynamic variation of alkaloids of BRC in the body exhibited the similarity to the pattern of its antifebrile effect.
Administration, Oral
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Alkaloids
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pharmacokinetics
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Animals
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Bile
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Coptis
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chemistry
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Drugs, Chinese Herbal
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chemistry
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pharmacokinetics
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Rats
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Rats, Sprague-Dawley
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Rhizome
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chemistry
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Tandem Mass Spectrometry
9.Research status of conbercept treating age-related macular degeneration
International Eye Science 2015;(8):1367-1369
Age-related macular degeneration( AMD) is one of the major reasons of blindness among the elderly in the developed countries. As AMD patients are increasing year by year, AMD has become one of the important topics of ophthalmic research to prevent blindness. Its pathogenesis is not fully understood, but many studies have shown that vascular endothelial growth factor ( VEGF ) plays an important role in the pathogenesis. With the development and application of anti - VEGF drugs, there are a variety of drugs applied to the disease. This article introduces conbercept for the treatment of AMD.
10.Clinical Analysis of 10 Cases of Juvenile Rheumatoid Arthritis Associated Iridocyclitis
fang, CHEN ; zi-jiang, LI ; jing, LIU
Journal of Applied Clinical Pediatrics 2004;0(07):-
Objective To study the clinic features and prognosis of juvenile rheumatoid arthritis (JRA)-associated iridocyclitis.Methods Ten cases of JRA -associated iridocyclitis choosed from 107 cases with JRA were reviewed and analysed.Results Polyarticular( n =9) and pauciarticular( n =1) were observed among JRA -associated iridocyclitis.Four were acute cases and all were recovered.Secondary glaucoma ( n =1) and ablepsia( n =1) were find in the chronic cases of 6,respectively.Conclusions Iridocyclitis is a common complication of JRA. Most of them are polyarticular group. The acute cases have better results compared to the chronic cases which often have more complications such as glaucoma,cataract, et al .