This study was aimed to analyze the volatile oil of Mentha crispata Schrad. ex Willd. in order to provide evidence for its chemotype and guidance for its production application. The chemical analysis was detected by headspace GC-MS. The results showed that 64 chemical compounds were detected. It was concluded that the volatile oil of M. crispata Schrad. ex Willd. mainly contained eucalyptol (35.58%), limonene (16.92%) and pinene (15.33%). It was concluded that the analysis on composition characteristics and main compounds of M. crispata Schrad. ex Willd. can provide evidences in its production application and chemotype.

Objective To investigate the effects of divitamins notonginseng and cinnarizine capsule(DNCC) on acute cerebral ischemia.Methods ICR mice were administered three doses of DNCC(420,210,and 105mg/kg;ig.) for ten days,then the gasping time after decapitation was recorded.After three doses of DNCC(292,146,and 73mg/kg;ig.) were given respectively for ten days in rats,the effects of DNCC on the infarct size,histological changes and neurological function scores caused by focal cerebral ischemia which was induced by middle cerebral artery occlusion were investigated.Results DNCC prolonged the gasping time of mice after decapitation and improved the neurological function scores,cerebral ischemia injury and decreased the infarct size in rats.Conclusion DNCC has a protective effect against acute cerebral ischemia.

This article was aimed to survey the use of Chinese minority medicine, in order to provide differences and similarities among different Chinese minority medicine, and to analyze the relations among species resources, using range, functions and indications. It provided a basic scientific platform for the development, research and use of minority medicine. The books and journals published since the late 1970s (the country after the reform and opening up) had been collected, classified. And reference books on current Chinese minority medicine had been compiled. The results showed that the first draft of the book had been completed and delivered to the press. The dictionary had cited 53 traditional medicine used by minority groups. The total number of minority medicine was 7 734. The total characters amount in the book was 1 700 000 words. It was concluded that the species of minority medicine were various. There was rich information on their harvest, medicinal parts, functions and indications with prominent features. The existing major problems were to clarify the standard of the species as well as their major functions and indications.

Objective To evaluate the clinical efficacy and safety of adapalene0.1%gel with individ-ualized treatment regimen for acne vulgaris(ITRAV).Methods Eighty-one patients with acne vulgaris of mild to moderate severity were treated with adapalene0.1%gel topically1to3times daily for6to8weeks according to the severity of the disease.Clinical responses were recorded and photographed weekly in30pa-tients randomly selected from the81patients,and treatment regimens were adjusted accordingly.Results It was shown that cure rates were44.4%and73.4%,in81recruited cases and30selected cases,respectively.Seborrhea decreased remarkably(83.3%)in the treatment.Side effects took place in39.5%of patients with-out interruption of the treatment.Conclusions ITRAV with adapalene0.1%gel has been proved to have an excellent response.Reasonably increasing the daily dosage could improve the cure rates.The cutaneous tolera-bility of the drug was generally good.Seborrhea could be reduced considerably during the treatment

This study aims at trying to establish a novel method of sterility test for injections based on biothermodynamics, in order to overcome the deficiencies of routine sterility tests such as long detecting cycle, low sensitivity and prone to misjudgments. A biothermodynamics method was adopted to rapidly detect the microorganism contamination of injections by monitoring the heat metabolism during the growth of microbe. The growth rate equal to or greater than zero and the heat power difference of P(i) and P(0) with three folds higher than the noise of baseline were chosen as indexes to study the heat change rule of microbe. In this way, the effectiveness of the new method to detect strains required by conventional sterility test or in injection samples was also investigated. Results showed that the Gram-positive bacteria, Gram-negative bacteria and fungi demanded by sterility testing methodology could be detected by biothermodynamics method within 10 hours, with the sensitivity lower than 100 CFU x mL(-1). Meanwhile, this method was successfully applied to the sterility test of Compound Yinchen injection (FFYC), Shuanghuanglian powder injection (SHL) and Compound Triamcinolone injection (TAND) which were sterilized with different degrees. Therefore, the biothermodynamics method, with advantages of fast detection and high sensitivity, could be a complementary solution for conventional sterility tests.

OBJECTIVETo study the morphological and anatomical characteristics of Coptis deltoidea based on the research of its breeding system.

METHODThe flowering process of C. deltoidea was observed dynamically and the out-crossing index was estimated, using Motic BA200 microscope to observe the morphologic characteristics of the mixed bud, stolon and pollen was observed microscopically, and compared with those of C. chinensis.

RESULTThe results showed that the breeding system of C. deltoidea was facultative hybridization, it could bloom normally and fruit while cannot form seeds. And there were no statistical differences in the number of stamens, pistils, ovules, pollens in a stamen, pollens in a flower, pollen-ovule ratio, and the pollen grain size between C. deltoidea and C. chinensis. However the pollen of C. deltoidea developed anomaly, most of the pollen grains in it were surface depression, the texture was unsharp or broken, the pollen could not germinate. The lateral bud on the lower side of the mixed bud formed in the bud stage. Pericyclic fibers in the stolon (the vegetative propagation branch) shaped like a cap, and all the shaped-caps nearly formed a ring.

CONCLUSIONThe abnormal development of the pollen could be the mainly cause to the no formation of seeds in C. deltoidea. The lateral bud forming in the bud stage then developing the stolon is the characteristics of the asexual propagation. Pericycle fibers in the stolon nearly forming a ring is a secondary character to accommodate the vegetative propagation of C. deltoidea.

Breeding ; Coptis ; anatomy & histology ; genetics ; growth & development ; Flowers ; anatomy & histology ; genetics ; growth & development ; Pollen ; anatomy & histology ; genetics ; growth & development

Objective To investigate the effect of high active antiretroviral therapy ( HAART) on growth and development of infants born to pregnant women infected with human immunodeficiency virus (HIV) and the effect on blocking mother to children transmission.Methods Totally 165 pregnant women diagnosed with HIV infection from May 2006 to May 2017 and their 169 infants, including four pairs of twins were enrolled, and 82 infants born to HIV negative pregnant women in the same period were enrolled as control .All of the pregnant women in the experimental group were administrated with HAART when HIV antibody test was positive.The delivery intervention and artificial feeding were carried out as well.The weight, height, hemoglobin (Hb), serum iron and serum calcium level of infants at birth , 12 months and six years old were monitored and compared between the two groups.Apgar scores of newborns and intelligence tests at six-year-old were also recorded.Statistical analysis was performed by t test.Results Pregnant women were generally in good conditions and well tolerated to the drugs.There were no significant differences in neonatal Apgar scores , body weight, body length, Hb, serum iron, serum calcium and CD4+T lymphocyte count between HIV positive experimental group and control group (t ＝-1.27,-1.12,-3.41,-5.62,-0.89,-3.02 and-0.74, respectively, all P＞0.05).At the age of 12 months, there were no significant differences in body weight , length, Hb, serum iron, serum calcium and CD4+T lymphocyte count between the two groups ( t ＝1.02, 1.41, 1.32, 1.03, 0.89 and 1.06, respectively, all P ＞0.05).At the age of six years, there were no significant differences in all indexes between the two groups (t＝1.02, 0.87, 1.58, 1.03, 0.92 and 2.07, respectively, all P ＞0.05).Intelligence assessment was performed in 78 children of the experimental group and 45 children of the control group at the age of six years , and there was no significant difference between the two groups ((89.7 ±12.5) score vs (91.2 ±13.7) score, t＝1.67, P＝0.43).All the children in the experimental group were positive for HIV antibody at birth , and six cases were positive for HIV RNA who were diagnosed with neonatal HIV infection.HAART was initiated for the six cases , while HIV antibody tests were still positive until the age of 18 months after HAART.The rest of the children′s HIV antibody tests became negative with the mother to children tramsission rate of 3.55%(6／169).Conclusion HAART could not only block mother to children tramsission of HIV , but also has no effect on growth and intellectual development of children during the observation period.

OBJECTIVETo analyze the content of tanshinone IIA in Radix Salviae Miltiorrhizae from different habitats are regularly altered and find out the deviation between the studies.

METHODThe data of the content of tanshinone IIA in samples of Radix Salviae Miltiorrhizae from different areas and related information since 1997 was collected and statistically analyzed.

RESULT AND CONCLUSIONThe content of tanshinone IIA in wild Salvia was generally higher than that in the cultivated Salvia, and the content in nearly half cultivated Salvia was less than 0.2%. There existed a big difference between the used statistical model, and the data related the content in samples from Sichuan was the most divisive. The coefficient of variation of wild Salvia was below the cultivated Salvia, and the cultivated Salvia from different areas showed different coefficients of variation. The content of tanshinone IIA was lower when samples were extracted using the heating reflux method.

Diterpenes, Abietane ; Phenanthrenes ; analysis ; Salvia miltiorrhiza ; chemistry

OBJECTIVETo study the characteristics of soil microbial variation during Salvia miltiorrhiza crop rotation.

METHODthe conventional cultivating microbial method was used to study the microbial number and communities structure and soil microbial biomass phosphorus (SMBP) was determined by chloroform vapor extraction method. The data was then analyzed by SPSS software.

RESULTWith the increase of the crop rotation years, the numbers of bacteria and actinomycetes in soil also, but the fungi and SMBP decreased.

CONCLUSIONMicrobial mechanism of crop rotation of the planting S. miltiorrhiza is the regulation of microbial number and bacteria physiological communities, the process rebuilds the soil ecological system balance. Microbial communities in soil need at lest 2 years to get to restore, after planting S. miltiorrhiza, which consisting with traditional planting experience.

Actinobacteria ; isolation & purification ; Bacteria ; isolation & purification ; Plants, Medicinal ; growth & development ; metabolism ; microbiology ; Salvia miltiorrhiza ; growth & development ; metabolism ; microbiology ; Soil Microbiology

OBJECTIVETo investigate the history of the medicinal uses, resources, distribution, habitat and population characteristic of Coptis deltoidea, and provide basis for the protection and rational development and utilization of Radix Coptidis Deltoideae.

METHODThe relevant literature and data was scrutinized and herbarium was compared, interview and field survey methods were carried out.

RESULTThe medicinal history, resources, distribution, population characteristic and protective strategy of C. deltoidea were summarized. The sustainable development of C. deltoidea was discussed.

CONCLUSIONThe resource is endangered, the germplasm resources should be intentionally protected and ensure the sustainable development and utilization of C. deltoidea.

Conservation of Natural Resources ; methods ; Coptis ; growth & development ; Drugs, Chinese Herbal ; metabolism ; Ecosystem ; Endangered Species