1. Bone marrow mesenchymal stem cells and tongue squamous carcinoma cells: Promoting growth or targeting biotherapy
Chinese Journal of Tissue Engineering Research 2020;25(1):128-132
BACKGROUND: Currently, the treatment methods for tongue squamous cell carcinoma mainly adopt comprehensive sequence therapy mainly based on surgery, but the therapeutic effect is still unsatisfactory. In recent years, tumor targeted biotherapy has attracted increasing attention. Stem cell technology enables therapeutic factors to be selectively delivered to tumor sites to play corresponding roles. Bone marrow mesenchymal stem cells show great advantages in this respect. However, the effect and action mechanism of bone marrow mesenchymal stem cells on tongue squamous cell carcinoma are still unclear. OBJECTIVE: To review the research progress on the effect of bone marrow mesenchymal stem cells on tongue squamous carcinoma cells, and clarify its function and discuss its mechanism. METHODS: The computer was used to search literatures about bone marrow mesenchymal stem cells in PubMed database, Wanfang database and CNKI database. The key words were “bone marrow mesenchymal stem cells, tumor cells”, “bone marrow mesenchymal stem cells, tongue squamous cell carcinoma”, “bone marrow mesenchymal stem cells, proliferation”, “bone marrow mesenchymal stem cells, migration, invasion” in Chinese, and “bone marrow mesenchymal stem cells, cancer cells” and “bone marrow mesenchymal stem cells, tongue squamous cell carcinoma”, “bone marrow mesenchymal stem cells, proliferation”, “bone marrow mesenchymal stem cells, migration, invasion” in English. A total of 43 articles were included for analysis. RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells have strong proliferation characteristics and can migrate directionally and promote invasion of tongue squamous carcinoma cells, suggesting that even if bone marrow mesenchymal stem cells have the function of promoting defect repair, and can also express therapeutic factors by gene modification and migrate to tumor growth site to play a therapeutic role, they cannot be used for repair treatment of postoperative defects of tongue squamous cell carcinoma. Targeted biological therapy of mesenchymal stem cells is a brand-new and promising field, but its safety still needs to be taken into account, and its mechanism of action and potential risks still need our continuous exploration.
2.Preparation of RGD-targeted Microbubble Contrast Agent and Its Adhesion Efifcacy
Xiuxia XU ; Zhuqing SONG ; Jianrong XU ; Qiao HU
Chinese Journal of Medical Imaging 2015;(2):87-90
PurposeTo develop a novel RGD microbubbles (RGD-MBs) and to evaluate the targeted binding effect with endothelial cells in vitro.Materials and MethodsThe RGD peptide was coated onto the microbubbles through biotin-avidin linkage including 10 μg/ml and 30 μg/ml groups. The microbubbles not carrying RGD peptide were obtained as negative control. Blocking studies were performed with pre-incubation of the cells with RGD peptide for 2 hours. The microbubbleswere characterized by Accusizer 780 and optical microscope. The binding specificity of RGD-MBs withανβ3-expressing mouse endothelial cells was determined with competitive inhibition experiments in vitro. The attachment study was performed using parallel plate flow chamber to investigate the dynamic adhesion on endothelial cells at various shear stresses.ResultsThe RGD-MBs had an average diameter of (4.09±0.07) μm. The binding RGD-MBs per cell were 2.98±0.35 for 10 μg/ml RGD and 1.78±0.23 for 30 μg/ml RGD. RGD-MBs binding to mouse endothelial cells decreased 54.64% and 67.00% in the presence of RGD peptide at a concentration of 10 μg/ml and 30 μg/ml respectively. When the shear stress was under 1.5 dyne/cm2, the accumulation rate was increased with the increase of shear stress (P<0.05). Accumulation rate reached the maximum (48.72±4.26) RGD-MBs/min at wall share stress of 1.5 dyne/cm2, and decreased as sheer stress >1.5 dyne/cm2 (P<0.05). Conclusion The RGD-MBs can specifically bind to endothelial cells, indicating its usefulness as ultrasonic molecular probe in monitoring integrinανβ3 expression during tumor angiogenesis, and is potentially valuable for in tumor early-staging and prognosis.
3.Self-treatment on benign paroxysmal positional vertigo
Xuhua CHU ; Huailin XIA ; Binnong WANG ; Zhuqing XU
Chinese Journal of Neurology 2001;0(02):-
Objective To compare the efficacy of a self-applied modified Semont maneuver(MSM) with self-treatment using a modified Epley procedure(MEP) in patients with posterior canal benign paroxysmal positional vertigo(PC-BPPV).Methods All 70 patients with PC-BPPV were treated respctively by using MEP and MSM between July 2001 and June 2003,remission rate and treatment-related side effects were compared at the end of 1 week.Results Remission rate was 95% in the MEP group(n=37) vs 58% in the MSM group(n=33,P
4.THE SILENCE OF SHORT HAIRPIN RNAs INDUCED Smad2 IN NIH/3T3 FIBROBLAST CELLS
Rong ZHENG ; Qi XIONG ; Siwen JIANG ; Bo ZUO ; Fenge LI ; Dequan XU ; Zhuqing REN ; Yuanzhu XIONG
Acta Anatomica Sinica 2002;0(05):-
Objective To construct five shRNA-expression plasmids and to investigate the expression of Smad2 in TGF-?/ Smads signal transduction treated with shRNA-expression plasmid.Methods Five shRNA-Smad2 DNA sequences from mRNA sequence of mouse Smad2 gene were designed and synthesized.DNA oligonucleotides encoding an appropriate shRNA were inserted to shRNA expression vector respectively.Five shRNA-Smad2 expression plasmids were obtained and then transfected into NIH/3T3 cells.The suppressed expression of Smad2 was assessed by RT-PCR and Western-blotting.Results The shRNA-expression plasmid numbered 2.4 could markedly reduce the expression of Smad2.The suppression effect of the RNAi-pool composed of four different plasmids was more obvious than that of any single.Conclusion The shRNA-expression plasmids were successfully constructed,which could specifically and effectively suppress the expression of Smad2.The method of using a mixture of RNAi plasmids to improve the RNAi efficiency was established.
5.Visualization of serotonin 1A receptor trafficking in neuron-like PC12 cells
Yanyan JIN ; Qiong LU ; Zhuqing YAN ; Erjing GAO ; Chunli ZHAO ; Jinlu ZHANG ; Zhiqing XU
Basic & Clinical Medicine 2010;30(5):487-491
Objective To explore the mechanisms of trafficking and signaling of serotonin 1A receptor(5-HT_(1A))and its spatiotemporal distribution in living cells.Methods The mouse 5-HT_(1A) gene amplified by RT-PCR was recombined into pEGFP-N1 vector and the EGFP coding sequence was located in-frame at the C-terminal end of the 5-HT_(1A) receptor.The 5-HT_(1A)-EGFP was transfected into neuron-like PC12 cells as well as HEK293.The transfected cells were visualized using confocal microscopy,the mobility of 5-HT_(1A)-EGFP was monitored by live measurements and fluorescence recovery after photobleaching.Results The 5-HT_(1A) gene was identitical with the published gene sequence NM_008308.4 and a 5-HT_(1A)-EGFP fusion construct was created.After stable transfection of the plasimd into a PC12 cell line and analysis with a confocal laser scanning microscopy,the EGFP-tagged 5-HT_(1A) was predominantly associated with the plasma membrane,but some intracellular vesicles in the perinuclear region also contained the fusion protein.The predominant localization of 5-HT_(1A)-EGFP at the plasma membrane was confirmed in transiently transfected HEK293 cells.Bleached fluorescence was partialy recovered in 100 seconds,indicating that the 5-HT_(1A)-EGFP was mobiled on the membrane.Conclusion Spatiotemporal distribution and mobility of 5-HT_(1A) tagged with EGFP can be monitored in the 5-HT_(1A)-EGFP stable PC12 cell line,which could be an excellent neuron-like experimental cell model for research of 5-HT_(1A) trafficking and signaling.
6.Graphical modeling of ICF Core Set (Comprehensive Version for Stroke)
Meng YOU ; Zhuqing JIANG ; Xu WANG ; Shengli DI ; Fengqin ZHANG ; Zhaoming GUO ; Jian XIANG ; Lin CHANG ; Tiantong YANG
Chinese Journal of Forensic Medicine 2016;31(4):336-340
Objective It aims to investigate the relationships among the categories of Comprehensive Version for Stroke as described in the International Classiifcation of Functioning, Disability and Health (ICF) Core Set, and to provide new supports for Judicial Appraisal of functioning in stroke by ICF functioning mapping.Methods The variables of 59 categories of ICF assessment scale and the samples of 106 persons’ are selected and used in the least absolute shrinkage and selection operator (LASSO) for mining dependencies among those variables. The graphical modeling and analyzing with the software Gephi provides a visual map of the correlations among those classiifcations. Results 59 interconnected categories which organized into the functioning mapping. b340, b735, b175 and b152 are centrally positioned categories because of their high correlation.Conclusion Functioning mapping by graphical modeling can reveal complex relational structures embedded in functioning classiifcations, which provides the support for using ICF to appraisal stroke.
7.Correlation between postmortem intervals and the changes of 18s rRNA degradation in liver after death under the ;condition of different temperature(
Zhuqing JIANG ; Dong ZHAO ; Haidong ZHANG ; Xu WANG ; Meng YOU ; Xi CHEN ; Xiaoxu ZHU ; Wei ZHONG ; Shi FENG ; Lin CHANG ; Tiantong YANG
Chinese Journal of Forensic Medicine 2016;31(5):441-443
Objective To investigate the relationship between Ct value of mice liver and postmortem interval (PMI) under various ambient temperatures. Methods mice were stored at 10℃, 15℃, 20℃, 25℃ and 30℃ after execution, and total RNA was extracted from mice liver every 6 hours (PMI 6h to 72h). The levels of 18s rRNA were examined using real-time PCR. The results were expressed by cycle threshold (Ct) value to explore relationship between PMI and Ct value, and the interpolation functions were established to estimate PMI. Results In each group, Ct value increased with PMI increased. Surface equation was obtained after interpolation analysis on temperature range 10℃~30℃. The three-variable quintic surface equation was f(x, y)=-426.9+30.82x+44.48y-1.297x2-1.837xy-1.388y2+0.034 38x3+0.038 17x2y+0.038 67xy2+0.028 77y3-0.000 612 9x4-3.897e-7x3y-0.001 223x2y2+0.000 256 6xy3-0.000 537 4y4+3.606e-6x5-2.846e-6x4y+1.009e-5x3y2-3.439e-6x2y3-2.556e-7xy4+2.664e-6y5(r2=0.999 4). Conclusion The rule of Ct value changes at ambient temperature complied with three-variable quintic surface equation distribution. Measurement of interpolation function may be used for PMI estimation at ambient temperature.
8.Spine and extremities impairment: comparative study on clause for Identification of the body injured and Classification of the body impairment
Siyang XIANG ; Lili YU ; Xu WANG ; Jian XIANG ; Meng YOU ; Zhuqing JIANG ; Tiantong YANG
Chinese Journal of Forensic Medicine 2017;32(5):518-521
The article is comparative study about spine and extremities on clause for the identification of the body injured and The classification of the body impairment. We reviewed the terms and provisions about spine and extremities as follows, amputation impairment, function impairment, and amputation impairment combined function impairment. This paper provides a comprehensive access and analysisofthe similarities and differences between the two standards.
9.A study on detection of α-helix protein in posttraumatic epileptogenic focus by FTIR-mapping
Siyang XIANG ; Zhuqing JIANG ; Jiangtao LI ; Meng YOU ; Haidong ZHANG ; Xu WANG ; Hongxia LIU ; Tiantong YANG
Chinese Journal of Forensic Medicine 2017;32(6):583-587
Objective The article is to study on the detection of α-helix proteins in post-traumatic epileptogenic focus by FTIR-mapping. Methods Fourier transform infrared spectroscopy-mapping were applied to identifying α-helix by point-by-point scanning in post-traumatic epileptogenic focus sections and to develop FTIR-mapping profiles. Result The high absorbance of α-helix is accord with post-traumatic epilepsy, there are some significant differences between high absorbance and low absorbance. Conclusion α-helix proteins are distributed in post-traumatic epileptogenic focus widely, thus α-helix protein are involved in post-traumatic epilepsy.