OBJECTIVE:To establish a RP-HPLC method to simultaneously determine the contents of piperacillin and taz_obactam METHODS:Using ? Bondapak C18 column as fixed phase,a mixture of methanol and purified water(34 5∶65 5)as mobile phase,and caffeine as internal standard,detecting wavelength:220nm,flow rate:1 0ml/min RESULTS:The linear rangs of piperacillin and tazobactam were 40～200?g/ml and 5 0～25 0?g/ml respectively The average recovery and RSD (n=5) were (100 2?0 61)% for piperacillin and (99 03?0 90)% for tazobactam CONCLUSION:This method is rapid,simple and accurate

Objective:To investigate the combining therapy which not only have cured effect but also can uphold and improve the NPC patient′s immunity function after radiotherrapy and chemotherapy.Method:90 cases randomly divided into 3 groups ①Local group (local injected with IL-2 +radiotherapy+chemotherapy);②General group(ivdrip with IL-2+radiotherapy+chemotherapy);③convention group(radiotherapy+chemotherapy).Checked and observed the immunity function around the immunotherapy and after the radiotherapy and chemotherapy.Result:Cellular immunity of 3 groups are lower and humoral immunity are hypetuntion than normal person.After treated with IL-2 the cellular immunity improves but there′s no great change of the humoral immunity. The immune status of the immune groups have not obvious change than before radiotherapy,at the same time,the cellular immunity of the convention group cut down and the humoral immunity doesn′t change obviously.Conclusion:①It has some effect to uphold and improve the NPC patient′s immunity function to treat with small dosage of IL-2 before radiotherapy and chemotherapy,general treatment is better than local injection;②The three therapies have not great influence on the patient′s humoral immunity.