Objective To test the allergen‐specific‐IgE in different districts for bronchial asthma children ,and analyze the re‐sults .Methods The blood samples of bronchial asthma children which selected from Heyuan City and Shenzhen City were detected by using German Ormond Western Blot Method reagents .Results The total positive rate of bronchial asthma children′s allergen‐specific‐IgE from Heyuan was 61 .7% .The positive rate of inhalation allergen was 43 .4% .The top three of inhalation allergens were house dust(41 .7% ) ,combination of mole(33 .3% ) ,the dog epithelium(20 .0% ) .The positive rate of food‐borne allergen was 36 .7% .The top three of food‐borne allergens were milk(33 .3% ) ,peanut(23 .3% ) ,beef(11 .7% ) .The total positive rate of bron‐chial asthma children′s allergen‐specific‐IgE from Shenzhen was 86 .7% .The positive rate of inhalation allergen was 73 .3% .The top three of inhalation allergens were combination of mole (63 .3% ) ,house dust (61 .7% ) ,The Liu/Yang/Elm combination (33 .3% ) .The positive rate of food‐borne allergen was 58 .3% .The top three of food‐borne allergens were shrimp(31 .7% ) ,crab (28 .3% ) ,the cod/lobster/scallop combination(21 .7% ) .Conclusion There were different parterns of the positive rate of bronchial asthma children′s allergen‐specific‐IgE in different districts .

OBJECTIVE:To establish a method of determination of midecamycin A1 in midecamycin tablets METHODS:This method used C18 column as stationary phase,0 1mol/L ammonium formate solution-acetonitrile(80∶55) as mobile phase and 232nm as detection wavelength RESULTS:There was a good linear correlation in the range of 0 5～8mg/ml,r=0 9 995,S=5 989C-24 65,RSD=1 4%(n=5) The mean recovery was 99 35% and RSD=1 2%(n=6) CONCLUSION:The method is simple and easy to do and may be adopted for quality control of this drug

Objective To assess the efficiency of algae extermination by the combined copper electrolysis technology. Methods The combined copper electrolysis technology and copper sulphate were used to treat the Synechocystissp strain PCC6803 in the steady stage of culture in different time and the efficiency of algae extermination was assessed. Results No alga in the water sample was detected after 0.5 and 1 hour of treatment with copper electrolysis technology and copper sulphate respectivly as the concentration of Cu2+ was at 0.3 mg/L. The extermination rate of algae was 80.75% after 12 days of treatment of combined copper electrolysis. This technology could effectively decrease the turbidity, smell, COD, ammonia nitrogen and increase the dissolved oxygen in the treated water. Conclusion The Cu2+ released by copper electrolyzing has a stronger efficiency of algae extermination compared with copper sulfate. The combined copper electrolysis technology will be a satisfactory method for algae extermination in the landscape water.

Objective:To investigate the infuluence of electroacupuncture on blood lipid and liver function in nonclcoholic fatty liver rats. Methods:Thirty-three male SD rats were randomly divided into 2 groups:normal control group (n=11,fed with normal food) and non-alcoholic fatty liver model group (NAFLD) (n=23,fed with high-fat food). Eight weeks later 1 rats were randomly selected from the normal control group and 2 rats were randomly selected from the NAFLD group and were killed to undergo pathological examination of the liver. When the establishment of experimental model of NAFLD rats was confi rmed the remaining 20 NAFLD rats were sublishment into 2 equal subgroups:NAFLD control group to be fed continuously with high-fat food ,acupuncture treatment group (to fed continuously with high-fat food). Take Zusanli,Hong Leong,Sanyinjiao,Taichong points,electroacupuncture -spacing parameters,2Hz frequency,intensity 4mV to the appropriate vibrating slightly lower limb. By the end of the 12th week,all rats were killed to isolate the samples of test the levels of serum total cholesterol (TC),serum triglyceride (TG),high-density lipoprotein cholesterol (HDL-L),low-density lipoprotein cholesterol (LDL-CH),liver index,Valley serum ALT(ALT),Aspartate aminotransferase (AST),and liver pathology. Results:Treatment of rats --serum total cholesterol (TC),serum triglyceride (TG),low-density lipoprotein cholesterol (LDL-CH),liver index,Valley serum ALT(ALT),Aspartate aminotransferase (AST) were significantly lower than those of NAFLD control group (P

Objective To investigate the mechanism of electroacupuncture in the treatment of non-alcoholic fatty liver disease(NAFLD).Methods Thirty-three male SD rats were randomized into 2 groups:normal control group(n=11,fed with normal food)and experimental rats(n=23,fed with high-fat food).Eight weeks later,3 rats were randomly selected from the 2 groups respectively and killed to undergo pathological examination of the liver.When the establishment of experimental rats was confirmed,the remaining 20 experimental rats were divided into 2 equal subgroups:NAFLD group(fed continuously with high-fat food)and electroacupuncture treatment group(fed continuously with high-fat food and received the treatment 15 min per day,once a day for 4 weeks).Then all rats were killed to detect the serum levels of fasting blood glucose(FBG),fasting insulin(FINS)and free fatty acids(FFA),liver contents of malondialdehyde(MDA),glycerin tripalmitate(TG)and total cholesterol(TC),and the activities of liver superoxide dismutase(SOD)and glutathione(GSH).Liver pathological changes was examined.Results Compared with NAFLD group,electroacupuncture reduced the serum levels of FBG,FINS and FFA(P

Biotechnological pharmaceutics is an important course for pharmacy undergraduates , however there are many problems in current curriculum design and teaching methods. With the advantage of our platform National Engineering Research Center of Immunological Products, we tried to carry out the teaching reformation based on Excellent Engineer Education and Training Plan. We optimized the curriculum standards and teaching design, highlighted the combination ofBiotechnological pharmaceuticsand Engineering, strengthened the experiment teaching, tried the reformation of teaching method such as flipped classroom and PBL, strengthened the cultivation of innovative thinking and scientific research ability. Our teaching reformation is beneficial to cultivating the compound talents in the field of biotechnological pharmaceutics.

This paper analyzed innovative education management system,innovative education resources,innovative education mode and innovative education evaluation standard through practicing‘integration of production,study and research’ teaching platform. Meanwhile,this paper explored the optimization system of innovative education in medical undergraduate teaching.

Objective To evaluate a model of management for chronic pain disease coordinated community health services and district hospital. Methods The epidemiology of chronic pain disease was surveyed in Haotao community of Zhongshan, before and after the establishment of the model of management. Results The prevalence rate of chronic pain disease was 51.83%, in which it was 47.06% in men, and 57.32% in women. After the establishment of the model, the efficacy of good to excellent improved from 30.22% to 67.08%, the cost for medicine reduced 36.16%, time for rest reduced from 32.42 d to 15.25 d (P<0.05). Conclusion The model of management that coordinated community health services and district hospital, including demonstration diagnosis and treatment through internet, tele-consultations and health education, is effective on chronic pain disease.

Objective To expore the clinical therapeutic effect of allergic rhinitis using endoscopic more nasal structures orthopedic surgery combined with steroid nasal spray. Methods Sixty allergic rhinitis patients accompanied by deviation of nasal septum, middle turbinate lesions, or/and inferior turbinate lesions, were divided into two groups: observation group with 30 cases, applying endoscopic more nasal structures orthopedic surgery combined with steroid nasal spray (mometasone furoate nasal spray) during perioperative period; control group with 30 patients, only applying steroid nasal spray (mometasone furoate nasal spray). Long-term effect was compared between two groups. Results Sixty cases were followed up for 1 year. The total effective rate was 90.0%(27/30) in observation group,22 cases of significant effect, 36.7% (11/30) in control group,3 cases of significant effect. The total effective rate had significant difference between two groups (P＜0.01). Conclusion Using endoscopic more nasal structures orthopedic surgery combined with steroid nasal spray (mometasone furoate nasal spray) to treat allergic rhinitis has good long-term effect.

Objective To investigate the related mechanism of ligamentum flavum (LF) hypertrophy in diabetic pa-tients with lumbar spinal canal stenosis ( LSCS ) .Methods Twenty-four diabetes mellitus patients [ DM (+) ] and twenty normoglycemic patients [ DM (-) ] with LSCS were enrolled in this study .Sorbitol in LF was analyzed using D-Sorbitol/Xylitol test kit .The thickness of LF was measured by CT .The structure of LF was observed after HE and Masson's trichrome staining .The cell cycle and proliferation of fibroblastic cell NIH 3T3 line cultured in high glucose were analyzed .Sorbitol of NIH3T3 was detected under different backgrounds in vitro, normal glucose , high glucose and high glucose burdened with aldose reductase inhibitor ( ARI) , Epalrestat .The expression of inflammatory factors was detected by qPCR and Western blot under above different backgrounds .Results LF of diabetic patients exhibi-ted significantly higher level of sorbitol and pro-inflammatory cytokines , TGF-βand of CD68-positive staining than that of the normoglycemic subjects ( P<0.01 ) .The diabetic LF was significantly thicker than that of the controls , and showed evidence of degeneration .The high glucose-cultured fibroblasts exhibited significantly higher levels of sorbitol , pro-inflammatory factors , and TGF-βcompared to the low glucose-cultured cells , and these levels were dose-dependently reduced by treatment with the aldose reductase inhibitor (P<0.05).Conclusions Sorbitol level of the LF is significantly increased in the DM patients with LSCS .Increased sorbitol recruites inflammatory factors and fibrogenic-related factor TGF-βin LF of DM patients with LSCS which may contributes to the LF hypertrophy .