Objective:To develop a materials management information system for military hospital warehouse of combat readiness based on RFID so as to enhance the management of military medical corps for materials of war preparation.Methods:RFID(Radio Frequency Identification) tags were attached on war materials and the information could been read by bar-code reader. SQL Server 2005 was used as the backend database, and VC# was combined with RFID to develop the software system.Results: This research has developed a materials management information system for military hospital warehouse of combat readiness based on RFID and this system could achieve informatization for information management, management of out and in warehouse, emergency plans management and forewarning management. Through manoeuvre, the average time of war materiel out warehouse has saved 12min.Conclusion: The research and development of information management system for materiel information of hospital warehouse for combat readiness has reference value for the application of researching RBID technique in material management.

Objective To investigate the optimum techniques for processing Yuhuanlian with microwave. Methods Nine samples were obtained by orthogonal design. Thin-layer chromatography was used to determine berberine in Yuhuanglian. The content of berberine was taken as the index for the evaluation on the superiority of processing techniques. Results The optimal processing techniques for Yuhuanglian with microwave was suggested as follows:A2B2C3, moistened for 90 minutes, put 2 cm thick, with 80% of the microwave, heating 3 minutes. Conclusion The method is simple, easy to control, easy to use, easy to promote, and the quality of processed products is better.

Objective To explore the optimal method and condition for controlling release of vincristine (VCR) from VCR-loaded erythrocytes and enhancing the stability of carrier erythrocytes. Methods Erythrocytes were loaded into human erythrocytes using the modified hypotonic pre-swelling and isotonic resealing technique, and then with VCR, and they were treated with glutaraldehyde in concentration of 0.16% and 0.25% respectively. After storing at 4℃ for indicated time, the amount of VCR was determined with HPLC to compare their drug-release rate, and the degree of hemolysis in the supernatants was observed to compare the fragility of VCR-loaded erythrocytes treated with 0.16% glutaraldehyde with that with 0.25%, VCR-loaded erythrocytes treated with PBS, and VCR-loaded erythrocytes exposed to different hypotonic sodium chloride in different concentrations. Results The VCR-accumulated release rate increased in glutaraldehyde-treated VCR-loaded erythrocytes as well as untreated group during preservation. The VCR-accumulated release rate of 0.25% glutaraldehyde-treated group decreased by 71.67?4.20%, which was significantly slower than untreated VCR-loaded erythrocytes and 0.16% glutaraldehyde-treated group. After storing at 4℃ for 21 days, no significant changes at erythrocyte morphology was found in VCR-loaded erythrocytes treated with 0.25% glutaraldehyde, whereas untreated VCR-loaded erythrocytes and those treated with 0.16% glutaraldehyde can be stored only for 5d and 7.5d respectively. As compared to un-treated VCR-loaded erythrocytes, no significant change of osmotic fragility was seen in 0.25% glutaraldehyde-treated group, but significant increase in osmotic fragility was seen in 0.16% glutaraldehyde-treated group. Conclusion 0.25% glutaraldehyde could optimally block the membrane of VCR-loaded human erythrocytes and enhance their stability.

This article expounds the existing situations in which the multimedia courseware has been applied widely in the institutions of higher learning,and emphasizes the importance and necessity of establishing and improving the assessment system of multimedia courseware.It forwards the principles of establishing the assessment criterion to be scientific,educative,technical,artistic and assistant,and furthermore lists measures to prepare and operate courseware,proposals to assess the teaching effectiveness.

Purpose:To evaluate the thrombolytic effect of transcatheter intra-arterial infusion of urokinase injectio salviae miltiorrhizae and heparine.Materials and methods:A 5F catheter was delivered to the thrombotic site via femoral artery,then infused urokinase.Injectio salviae mihionrrhizae via catheter.During and after the procedure heparin was given for antico- agulation.Finally to evaluate the effect by femoral arteriograms.Results:Blood circulation of 10 eases were inproved with complete pateney.One case wasn't very fluent and the last one remained obstructed.Total effective rate was 92%.Conclusion:Transcatheter Intraarterial thrombolysis by combing urokinase,injeetio salviae miltiorrhizae and heparin was a effective and safe theraputic method.

Depending on the elaboration work to the national drug standards, the writer gives some comments on how to carry out the monographs in Chinese Pharmacopoeia 2015 edition Volume II. The comments is in the items order of national drug standards. The writer also gives the reasons and considerations for some monograph's revision and elaboration so that the stockholders can understand and carry out the new edition pharmacopoeia quickly and exactly.

The author summarized the national drug standards of radiopharmaceutical preparations and analyzed the existing problems, and gave a brief introduction about the revision in the Chinese Pharmacopoeia 2015 edition.

OBJECTIVE:To study the protective effect of butylphthalide on the apoptosis of human umbilical vein endothelial cells (HUVECs) induced by Aβ1-42 and its mechanism. METHODS:HUVECs were divided into normal control group,Aβ1-42 group,TAK242 group(10 nmol/L),DMSO group(1‰DMSO)and butylphthalide low-concentration,medium-concentration and high-concentration groups (40,80,160 μg/L). Except for normal control group and DMSO group,other groups were given 50 μmol/L Aβ1-42 to culture HUVECs for 24 h. TAK242 group,DMSO group and butylphthalide low-concentration,medium-concentra-tion and high-concentration groups were given relevant concentration of drugs for 30 min,with 3 holes for each concentration. The cell viability was determined by CCK-8 assay;cell apoptosis was observed by Hochest 33342/PI double staining;the cell apoptotic rate was detected by AnnexinⅤ-fluorescein isothiocyanate (FITC) flow cytometry;the protein expression of TLR-4 and COX-2 were determined by Western blot assay;the contents of IL-1 and TNF-α were detected by ELISA. RESULTS:Compared with nor-mal control group,cell viability of HUVECs were decreased in Aβ1-42 group;while apoptotic rate,protein expression of TLR4 and COX-2,the contents of IL-1 and TNF-α were increased. Compared with Aβ1-42 group,cell viability of HUVECs were increased in TAK242 group and butylphthalide low-concentration,medium-concentration and high-concentration groups;while apoptotic rate, protein expression of TLR4 and COX-2,the contents of IL-1 and TNF-α were decreased,with statistical significance(P<0.05 or P<0.01). CONCLUSIONS:Butylphthalide can reduce HUVECs apoptosis induced by Aβ1-42,which may be related with inhibiting the expression of TLR4,COX-2 and inflammatory factors.

Humans ; Infection ; etiology ; therapy ; Pancreatitis ; complications ; therapy

Adult stem cells are the multi-potential cells, which exist in fetal and adult tissues. It can reproduce itself (undergo self-renewal) or give rise to more specialized (differentiated) cells. Under certain inducing conditions, adult stem cells can acquire the ability to differentiate into different tissue cells. Multipotent adult progenitor cells (MAPC), an alternative name of adult stem cell given by Catherine Verfaillie, existing in bone marrow, can differentiate into cells with characteristics of mesodermal, neuroectodermal, and endodermal lineages in vitro at the single-cell level. MAPC can also contribute to most cell types when injected into the blastocyst. Adult stem cell differentiation implies that different cell lineages are derived from a single initial cell; all differentiated cell types are functional in vitro and in vivo; and engraftment is robust and persistent in the physiological and pathological situations. The possible mechanisms may underlie the differentiation: various tissue-specific stem cells are present in different organs; adult stem cells would be reprogrammed when removed from their usual microenvironment and introduced into a different niche that imparts signals to activate a novel genetic program needed for the new cell fate. And true multi-potential stem cells persist in postnatal life. In the future, multi-potent adult stem cells might then be used for therapies of degenerative or genetic disorders of multiple different organs.
Adult Stem Cells ; cytology ; Cell Differentiation ; Humans ; Multipotent Stem Cells ; cytology ; Stem Cell Transplantation