Objective To study the necessity and feasibility of No.12b lymph node dissection in D2 lymphadenectomy for advanced distal gastric cancer,and the relation between No.12b lymph node metastasis and clinicopathologic factors.Methods Clinical data of sixty cases of advanced distal gastric cancer receiving D2 or D2+ radical correction were collected retrospectively,both of which were all plus No.12b lymph node dissections.The relationships between No.12b lymph node metastasis and clinicopathologic factors were analyzed.Results No death attributed to operation or severe operative complications were found.There were 12 cases(20.00%) with No.12b lymph node metastasis.The rates of No.12b lymph node metastasis in Borrmann Ⅲ-Ⅳ types,N2-3 of lymph node metastasis and T3-4 of tumor infiltration were 31.25%(10/32),30.30%(10/33) and 29.73%(11/37),which were significantly higher than those in Borrmann Ⅰ-Ⅱ types(7.14%(2/28)),N0-1(7.41%(2/27)) and T1-2(4.35%(1/23)) respectively(P

Mobilized peripheral blood stem cells (PBSC) have largely replaced the use of bone marrow as a source of stem cells for both allogeneic and autologous stem cell transplantation.G-CSF with or without chemotherapy is the most commonly used regimen for stem cell mobilization.Some donors or patients,especially the heavily pretreated patients,fail to mobilize the targeted number of stem cells with this regimen.The generation of new mobilization agents and new therapies can improve the mobilization of hematopoietic stem cells.

Objective To investigate the effect of combined spinal epidural anesthesia with sitting position in parturient woman undergoing elective cesarean section. Methods One hundred parturient women undergoing elective cesarean section were divided into two groups by random digits table, 50 cases of control group adopted combined spinal epidural anesthesia with left lateral position, 50 cases of observation group adopted combined spinal epidural anesthesia with sitting position, the onset time of anesthesia, operation time, dose of ephedrine, neonatal Apgar scores and side effects of two groups were compared and analyzed. Results Three cases of control group failed to be followed up,1 case of observation group performed urgent cesarean section.The onset time of anesthesia and dose of ephedrine of observation group was significantly shorter than that of control group, there was significant difference between two groups (P < 0.05 ); the operation time and neonatal Apgar scores of two groups were compared, there were no significant differences (P > 0.05); the incidence of side effect of observation group was significantly lower than that of controlgroup [ 14.3%(7/49) vs. 31.9%( 15/47)], there was significant difference (P< 0.05). Conclusion Combined spinal epidural anesthesia with sitting position in parturient women undergoing elective cesarean section has satisfactory results, shorter onset time of anesthesia, less dose of ephedrine, lower incidence of side effect, reducing the risk of maternal and neonatal anesthesia.

Pediatric venous thromboembolism is a life-threatening disease caused by many factors,of which the central venous catheter is the first high-risk factor,and the clinical presentation varies according to the position of obstruction.Color Doppler ultrasound,CT scan,MRI are the major diagnostic techniques.Low-molecular-weight heparin combines with oral anticoagulant agent are commonly used in therapy and prophylaxis.Early diagnosis and prevention are beneficial to the survival rate.

Thrombelastography(TEG) is a convenient,rapid test of coagulation.It records the continuous profiles of whole blood coagulation by measureing the viscoelastic changes of blood clot.Compared with conventional test of coagulation,TEG reflects the function of platelet,coagulation factors and fibrinolysis more comprehensively and dynamically.The small volume of blood required and less time-consuming make it more suitble for children.This review focuses on the clinical value of TEG in the diagnosis of hemorrhagic disorders as well as the individualized treatment of cogulation disorders,disseminated intravascular coagulation and antiplatelet drugs.

Objective:To investigate the effects of environment in vitro and in vivo on the differentiation of bone mesenchymal stem cells (MSCs) into neural cells.Methods:①MSCs were labeled with 4,6-diamidino-2-phenylindole (DAPI) and injected into the right brain of the rats(n=6),which had suffered from middle cerebral artery occlusion(MACO) a day before.Two weeks later,immunofluorescent staining was used to detect the expression of nestin,microtubule-associated protein 2 (MAP-2) and glial fibrillary acidic protein (GFAP).②In vitro,MSCs were cultured with modified neuronal medium(MNM)for 18 hours after being pretreated with 0.5umol/L all-trans retinoid acid (ATRA) for 24 hours.Immunocytochemistry was used to detect the expression of nestin,MAP-2 and GFAP.Results:①After being injected into rats for 2 weeks,MSCs survived in the ischemic areas and a few cells expressed nestin,MAP-2 and GFAP respectively.②In vitro,after ATRA and MNM treatment,the positive percentage of nestin and MAP-2 were (92.3?3.4)% and (89.6?3.3)% respectively,but no expression of GFAP was detected.Conclusion:The different ratio of MSCs differentiating into neural cells suggests that there is difference in the process and mechanism between environment in vitro and in vivo.

Objective To analyze and explore the clinical efifcacy of Yixinshu capsule combined with isosorbide dinitrate tablets in the treatment of stenocardia. Method 72 patients with stenocardia in cardiovasology of Zizhong county people’s hospital of Sichuan province were randomly divided two groups,36 cases in each group. Control group was treated with isosorbide dinitrate tablets, experimental group was given both Yixinshu capsule and isosorbide dinitrate tablets. Clinical efifcacy, adverse reactions and patients’satisfaction in two groups were analyzed comparitively. Results After treatment, the total effective rate and satisfaction degree in experimental group were signiifcantly higher than control group.Incidence of adverse reactions of experimental groupwas signiifcantly lower than control group(P<0.05). Conclusion Clinical efifcacy of Yixinshu capsule combined with isosorbide dinitrate tablets in the treatment of stenocardia is signiifcantly higher than single isosorbide dinitrate tablets, with higher satisfaction degree and lower incidence of adverse reactions, which can be widely applied in clinic.

OBJECTIVE: To establish the method for the determination of diosgenin in Di’ ao Xinxuekang Capsule by HP_LC. METHOD: The condition of HPLC was Shim-pack CLC-Sil columm ( 150mm? 6. 0mm) , mobile phase of 2. 0% isopropand in petroleum ether and UV detector at 206nm. RESULTS : The method proved to be linear in the range at 0. 58～ 11. 6? g of diosgenin with correlation coefficient of 0. 999 8. The linear formula was Y=3. 682? 107X+ 1700, The average recovery and recision were 99. 49% , and RSD=0. 12% ( n=5) . CONCLUSION: The method is simple, accurate, specific and can be used to the quality control of the preparation.

OBJECTIVE:To investigate the stability of Yanhouyan mixture(for pharyngolaryngitis).METHODS: The st-ability of Yanhouyan mixture was investigated by classic constant temperature accelerated test method and sample observation method taking the content of Baicalin as index which was determined by HPLC.RESULTS:The content change of Bacilin in Yanghouyan mixture was in line with the first order kinetics,and the test results for both methods were similar.Under room temperature(25 ℃),the validity duration of Yanhouyan mixture was 1.06 years.CONCLUSION:In establishing the quality standard for Yanhouyan mixture,the advisable storage life of 1 year for which should be considered.

This paper presents the classifications of the infection source areas for schistosomiasis japonica in the lake beach and embankment regions, systematically demonstrates the geo-graphical and endemic characteristics of different types of infection source areas. The results provide the basis for making control strategies of the disease.