1.Influence of Extract from T. Ramosissima on the Skin-wounded Mice
Zhujun MAO ; Fangzhou QI ; Yaokang XIONG ; Guidong DAI ; Luping QIN
Journal of Zhejiang Chinese Medical University 2013;(10):1222-1225
[Objective]To research the effect of acetic ether extract from T. ramosissima on the skin-wounded mice. [Methods]The acetic ether extract of T. ramosissima was administrated to skin-wounded mice by gavage methods. [Results] The experimental groups in different dosages had significant difference ,compared to the control group, with the areas of wound healing 17.68 mm2, 17.90 mm2, 20.18 mm2 and 31.48 mm2,after the acetic ether extract of T. ramosissima administration to skin-wounded mice by gavage methods. [Conclusions] It was suggested that the acetic ether extract of T. ramosissima had some protective effects on improving the wound healing.
2.Effects of ethanol extract of Rhizome Pinelliae Preparata on intracellular pH value of human gastric adenocarcinoma cells.
Cian ZHANG ; Feng WU ; Zhujun MAO ; Zhen WEI ; Yongjin LI ; Pinkang WEI
Journal of Integrative Medicine 2011;9(8):894-900
To observe the effects of ethanol extract of Rhizome Pinelliae Preparata on the intracellular pH value of human gastric cancer SGC7901 cells.
3.Molecular mechanism of astragalus polysaccharide in alleviating insulin resistance in HepG2 cells
CHENG Yue ; MAO Zhujun ; ZHANG Xin ; XIA Xufen
Journal of Preventive Medicine 2020;32(2):121-124
Objective:
To clarify the effect of astragalus polysaccharide (AP) on insulin resistance model of HepG2 cells induced by hyperinsulinemia and its underlying molecular mechanism in lipid metabolism and oxidative stress.
Methods:
HepG2 cells were divided into three groups: the control group was treated without any intervention; the model group was treated with 200 μL cell culture medium containing 10-6 mol/L insulin for 48 hours to build an insulin resistance model; the AP group was treated with optimal concentration of AP based on an insulin resistance model. After 24 hours, the concentration of H2O2 and the expression of PPARγ in each group were detected.
Results:
AP could improve the survival rate of insulin-resistant HepG2 cells in a dose-dependent manner. The highest survival rate of the cells was (118.26±1.17)% with 10 μM AP. The concentration of H2O2 in the AP group was (0.82±0.09) μM, which was lower than (1.30±0.16) μM in the model group (P<0.05), but was close to (0.78±0.09) μM in the control group (P>0.05). The relative mRNA expression of PPARγ in the AP group was 0.96±0.04, which was higher than 0.51±0.05 in the model group (P<0.05), but was close to 1.00±0.11 in the control group (P>0.05).
Conclusions
In the insulin resistance model in vitro, AP can significantly increase the cell survival rate, reduce intracellular H2O2 concentration, and promote the expression of PPARγ. The mechanism may be related to lipid metabolism.
4.Pharmacological Effect of Phellodendri Chinensis Cortex and Active Components on Gout: A Review
Min LI ; Yunyun QUAN ; Ting WANG ; Li LI ; Jin ZENG ; Junning ZHAO ; Jiuzhou MAO ; Yangfan TANG ; Zhujun YIN
Chinese Journal of Experimental Traditional Medical Formulae 2024;30(1):286-298
Gout is a metabolic disease closely associated with hyperuricemia and urate deposition. Because of the complex pathogenesis, high morbidity, multiple complications, and increasingly young patients, gout has received worldwide attention. Currently, western medicine mainly treats gout by lowering the uric acid level and reducing inflammation, which, however, causes serious adverse reactions and has contraindications. Phellodendri Chinensis Cortex (PCC) is the dried bark of Phellodendron chinense, with the effects of clearing heat, drying dampness, purging fire, detoxifying, and treating sores. Studies have shown that PCC and its active components have anti-inflammatory, pain-relieving, uric acid-lowering, and anti-gout activities, with extensive sources and high safety. PCC and its active components could prevent and treat gout through multi-targets and multi-pathways, whereas the systematic review remains to be carried out. Therefore, this paper summarized the pharmacological activities and mechanisms of PCC and its active components in the treatment of gout. The available studies have shown that PCC and its active components exert the anti-gout effect by lowering the uric acid level, reducing inflammation, alleviating oxidative stress, and regulationg intestinal flora, and protecting the kidneys. Particularly, the active components represented by alkaloids contribute obviously to the therapeutic effect of of PCC. Herein, we analyzed the problems and future development of the research on PCC, aiming to provide theoretical support and a scientific basis for the research and development of new drugs against gout.