1.Distribution of intravenously transplanted bone mesenchymal stem cells in ischemic brain and their effects on learning and memory in rats
Yan XU ; Jian ZHENG ; Zhujuan ZHOU
Journal of Third Military Medical University 2003;0(07):-
Objective To investigate the distribution and migration of bone mesenchymal stem cells (BMSCs) in the brain after intravenous injection into brain-ischemic rats and to explore the effects on the learning and memory in rats. Methods Bone mesenchymal stem cells from adult rats were cultured in vitro. Then the cells were labeled by Hoechst 33342 and infused into the adult rats on day 3 after the ischemia/reperfusion model was established by cerebral artery occlusion. Morris water maze test was used to explore the effects on learning and memory function of the rats at 3 weeks after transplantation. Fluorescence microscope was used to identify BMSCs in rat brains at 4 weeks after transplantation. Results All indices in Morris water maze test were significantly different from those of control group at 3 weeks BMSCs transplantation(P
2.Clinical effect of GnRH-α in treatment of patients with endometriosis after laparoscope surgery
Zhujuan HUANG ; Yeping WEI ; Yan HUANG
Chinese Journal of Primary Medicine and Pharmacy 2012;19(4):495-496
Objective To study the clinical effect of GnRH-α in treatment of patients with endometriosis after laparoscope surgery.Methods 185 endometriosis patients who got laparoscope surgery were randomly divided into the observation group(93 cases) and the control group(92 cases),and took GnRH-α and gastrinone,respectively.After 24 months follow-up,the outcomes of pelvic cavity symptom improvement,recurrence and pregnancy were observed.Results After treatment,the pelvic cavity symptom improvement (87.1% ) and pregnancy rate (79.3 % ) in the observation group was higher than those of the control group(70.7%,52.4% ) (x2 =7.517,4.059,all P <0.05),and the recurrence rate( 19.4% ) in the observation group was lower than that of the control group(34.8% )(x2 =5.581,P <0.05).Conclusion The treatment of endometriosis with GnRH-α combined with laparoscope surgery could attain desirable effect.Compared with gastrinone,it could effectively improve pelvic cavity symptom,decrease recurrence and increase the rate of pregnancy.
3.Analysis of tongue color under natural daylight based on chromatic aberration correction.
Jiatuo XU ; Zhifeng ZHANG ; Zhujuan YAN ; Liping TU ; Luming LU ; Meiyu SHI ; Fenglan ZHU
Journal of Integrative Medicine 2009;7(5):422-7
To establish an analytical method for tongue image acquisition under natural daylight based on L*a*b* error correction, and to observe the classification rules of tongue color using color error correction.
4.Correlation analysis of infrared radiant intensity between hyperplastic breast tissue and tongue surface in patients with hyperplasia of mammary glands
Zhifeng ZHANG ; Ziyan WANG ; Jian YING ; Jiatuo XU ; Xueyong SHEN ; Changle ZHOU ; Huayuan YANG ; Meiyu SHI ; Yun WU ; Zhujuan YAN
Journal of Integrative Medicine 2007;5(6):625-9
OBJECTIVE: To establish a supplementary diagnostic indicator (infrared radiant intensity) in tongue diagnosis of traditional Chinese medicine (TCM) in patients with hyperplasia of mammary glands through correlation analysis of infrared radiant intensity between hyperplastic breast tissue and tongue surface. METHODS: Infrared radiant intensity of the hyperplastic breast tissue and different points on tongue surface in 20 cases of hyperplasia of mammary glands with liver-energy stagnation and phlegm retention syndrome and 16 cases of hyperplasia of mammary glands with irregular thoroughfare and conception vessels syndrome were measured with external infrared spectrometer PHE-201 made by Shanghai Institute of Technical Physics. Correlation of infrared radiant intensity between the hyperplastic breast tissue and the different points on tongue surface was assessed by using bivariate correlation analysis. RESULTS: The results showed that the numbers of positive correlated wave bands of infrared radiant intensity between the hyperplastic breast tissue and different detected points on tongue surface in the patients with liver-energy stagnation and phlegm retention syndrome and irregular thoroughfare and conception vessels syndrome were 127 (83.55%) and 71 (46.71%), respectively. Infrared radiant intensity between the hyperplastic breast tissue and the tongue surface had a positive correlation. CONCLUSION: Infrared radiant intensity can be used as one of supplementary diagnostic indicators in TCM tongue diagnosis of hyperplasia of mammary glands.