Objective To test the reliability and validity of the short version of depression-anxiety-stress scale (DASS-21) for students in the disaster region.Methods DASS-21 and the Chinese version of UCLA PTSD Reaction Index for DSM-IV,Revision I were used to evaluate psychological health among 876 students from grades 5 to 9 after Ya' an earthquake.Results (1) Each of the items had a good distinction degree,with CR value ranging from 9.268 to 22.438 (P＜ 0.01),and the correlation coefficients ranged from 0.306 to 0.742.(2)The Cronbach' s alpha coefficients ranged from 0.781 to 0.911.(3) The correlation coefficients between total score and each dimension ranged from 0.714 to 0.914,and the correlation coefficient between PTSD and total DASS score,depression anxiety and stress score was 0.626-0.774.(4)Through the item analysis and explore factor analysis,the revised scale contained 21 items and 3 subscales which could explain 47.813％ of the total variance(KMO =0.937,x2 =3126.85,df=210,P＜0.01),and the results of confirmatory factor analysis supported the three-factor model (x2/df=4.180,P＜0.01,PCFI =0.728,CFI =0.904,IFI =0.905,TLI =0.881,and RMSEA =0.060),and the load of each item was between 0.339 to 0.715.Conclusion The short version of DASS-21 is reliable and valid,and can be used as a tool for post-disaster stress assessment on local students.

OBJECTIVE: To develop a noninvasive method for prediction of 1p/19q codeletion in diffuse lower-grade glioma (DLGG) based on multimodal magnetic resonance imaging (MRI) radiomics. METHODS: We collected MRI data from 104 patients with pathologically confirmed DLGG between October, 2015 and September, 2022. A total of 535 radiomics features were extracted from T₂WI, T₁WI, FLAIR, CE-T₁WI and DWI, including 70 morphological features, 90 first order features, and 375 texture features. We constructed logistic regression (LR), logistic regression least absolute shrinkage and selection operator (LRlasso), support vector machine (SVM) and Linear Discriminant Analysis (LDA) radiomics models and compared their predictive performance after 10-fold cross validation. The MRI images were reviewed by two radiologists independently for predicting the 1p/19q status. Receiver operating characteristic curves were used to evaluate classification performance of the radiomics models and the radiologists. RESULTS: The 4 radiomics models (LR, LRlasso, SVM and LDA) achieved similar area under the curve (AUC) in the validation dataset (0.833, 0.819, 0.824 and 0.819, respectively; P>0.1), and their predictive performance was all superior to that of resident physicians of radiology (AUC=0.645, P=0.011, 0.022, 0.016, 0.030, respectively) and similar to that of attending physicians of radiology (AUC=0.838, P>0.05). CONCLUSION Multiparametric MRI radiomics models show good performance for noninvasive prediction of 1p/19q codeletion status in patients with in diffuse lower-grade glioma.
Humans ; Magnetic Resonance Imaging ; Chromosome Aberrations ; Area Under Curve ; Glioma/genetics* ; ROC Curve

OBJECTIVETo investigate the expression of vascular endothelial growth factor (VEGF) in eutopic and ectopic endometrium of in nude mice models of endometriosis and understand the role of VEGF in the development of endometriosis.

METHODSNude mouse models of endometriosis were established by subcutaneous implantation and abdominal injection of human endometrium xenograft from patients with endometriosis. The morphology of the foci was observed microscopically, and VEGF expression was measured with immunohistochemistry.

RESULTSModels of endometriosis were successfully established in 8 of the 10 mice receiving subcutaneous xenograft implantation and in 7 of the 10 mice with abdominal graft injection. Increased VEGF expression was observed in the ectopic endometrium in these models.

CONCLUSIONVEGF expression is increased in the ectopic endometrium of the nude mouse models, and this animal model may facilitate future studies of angiogenesis and antiangiogenesis therapy.

Animals ; Disease Models, Animal ; Endometriosis ; metabolism ; Endometrium ; transplantation ; Female ; Humans ; Immunohistochemistry ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Middle Aged ; Transplantation, Heterologous ; Vascular Endothelial Growth Factor A ; biosynthesis

OBJECTIVETo construct a recombinant adenovirus carrying human endostatin gene with AdEasy system.

METHODSEndostatin gene fragment was amplified from Pshuttle-Endostatin plasmid with PCR and subcloned into the pAdTrack-CMV shuttle vector. The resultant plasimid was cotransduced into E.coli BJ 5183 cells with pAdEasy-1 plasmid for homologous recombination. The linearized recombinant plasmid was subsequently transfected into AAV 293 cells, and the recombinant adenovirus was detected by GFP, PCR and restriction analysis.

RESULTS AND CONCLUSIONThe positive clones of the recombinants were verified by restriction analysis and the titer of the virus reached 2.06 x 10(10)pfu/ml, suggesting successful construction of recombinant adenovirus pAd-Endo.

Adenoviridae ; genetics ; metabolism ; Cell Line ; Cloning, Molecular ; Endostatins ; genetics ; metabolism ; Escherichia coli ; genetics ; metabolism ; Gene Expression ; Genetic Engineering ; Genetic Vectors ; genetics ; metabolism ; Humans

HLA-Cw belongs to classic HLA-I gene, HLA-Cw molecules have high polymorphism like HLA-A and B molecules. They distribute extensively on the surfaces of karyote, not only presenting endogenetic antigen to CD8+ T cells to induce specific killing effect, but also participating in immunologic reaction as the ligands of killer cell immunoglobulin-like receptor (KIR). Thus it has been valued for their relations to diseases and the functions in transplantation immunity, anti virus and anti-tumor immunity.
Autoimmune Diseases ; immunology ; Graft vs Host Disease ; etiology ; HLA-C Antigens ; genetics ; physiology ; Humans ; Neoplasms ; immunology ; Receptors, Immunologic ; metabolism ; Receptors, KIR ; Virus Diseases ; immunology

OBJECTIVETo analyze the polymorphism and haplotypes of HLA class I and II in Guangdong Han population and detect the HLA-A, B, Cw and DRB1 allele frequencies.

METHODSAn auto semi-quantitative PCR-sequence speacific oligonucleotide probe(PCR-SSOP) method was adopted in exploring the HLA-A, B, Cw and DRB1 genotypes of the samples from 160 bone marrow donors.

RESULTSTwelve HLA-A, 23 B, 11 Cw and 13 DRB1 alleles were obtained. A total of 9 HLA-A-B, 20 Cw-B, 7 A-Cw, and 8 A-DRB1, 9 B-DRB1, 10 Cw-DRB1 haplotypes were found.

CONCLUSIONHLA class I and II alleles in Guangdong Han population have plenty of polymorphisms. The haplotype distribution possesses territory characteristic.

Asian Continental Ancestry Group ; genetics ; China ; Gene Frequency ; HLA Antigens ; genetics ; HLA-A Antigens ; genetics ; HLA-B Antigens ; genetics ; HLA-C Antigens ; genetics ; HLA-DR Antigens ; genetics ; Haplotypes ; genetics ; Humans ; Linkage Disequilibrium ; Polymorphism, Genetic

OBJECTIVETo investigate the effects of advanced oxidation protein products (AOPP) on epithelial-to-mesenchymal transition (EMT) in cultured human proximal tubular epithelial cells (HK-2) and explore the mechanism.

METHODSHK-2 cells treated with 50, 100, 200, and 400 µg/ml AOPP or 50 µg/m bovine serum albumin (BSA) for 24 h, or with 200 µg/ml AOPP for 0.5, 1, 3, 6, 12, and 24 h were examined for the protein expression of α-SMA and E-cadherin. In cells pretreated with diphenyleneiodonium (DPI) or cytoplasmic superoxide dismutase (C-SOD), the effects of 50 µg/ml BSA and 200 µg/ml AOPP were assessed on the expressions of α-SMA and E-cadherin, malondialdehyde (MDA) level, superoxide dismutase (SOD) activity, catalase (CAT) activity, and glutathione peroxidase (GSH-px) activity.

RESULTSAOPP treatment up-regulated α-SMA expression and down-regulated E-cadherin expression in a dose- and time-dependent fashion. AOPP exposure of the cells resulted in increased MDA level and lowered activities of SOD, CAT and GSH-PX. DPI and C-SOD partially attenuated the effects of AOPP on α-SMA, E-cadherin, MDA, SOD, CAT and GSH-px.

CONCLUSIONAOPP can induce EMT in cultured HK-2 cells via oxidative stress, and this effect can be attenuated by inhibiting the activation of NADPH oxidase and using antioxidants to delay the progression of renal interstitial fibrosis.

Actins ; metabolism ; Advanced Oxidation Protein Products ; Antioxidants ; metabolism ; Cadherins ; metabolism ; Catalase ; metabolism ; Cell Line ; Cells, Cultured ; Down-Regulation ; Epithelial Cells ; cytology ; Epithelial-Mesenchymal Transition ; Glutathione Peroxidase ; metabolism ; Humans ; Malondialdehyde ; metabolism ; NADPH Oxidases ; metabolism ; Oxidative Stress ; Superoxide Dismutase ; metabolism ; Up-Regulation

OBJECTIVETo investigate the association between single nucleotide polymorphisms (SNPs) of the transmembrane protein 39A (TMEM39A) at the loci 1880G/A, 2442T/G, and 2456A/T and systemic lupus erythematosus (SLE) in Chinese Han patients.

METHODSTMEM39A gene polymorphisms at 3 loci (1880G/A, 2442T/G, 2456 A/T) were analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 110 Chinese Han patients with SLE and 80 normal control subjects, and the allele and genotype frequencies were compared by Chi-square test between the two groups.

RESULTSBoth the genotype frequencies (AA, GA and GG) and allele frequencies (A and G) at 1880G/A differed significantly between SLE cases and the normal controls (P=0.002 and P=0.044, respectively). The two groups also showed significant differences in the genotype frequencies (GG, TG and TT) (P=0.001) and allele frequencies (G and T) (P=0.041) at 2442T/G. No significant differences were found in the genotype frequencies (TT, AT and AA) or allele frequencies (T and A) at 2456A/T between the two groups (P>0.05). The allele and genotype frequencies of the 3 SNPs showed no significant differences between lupus nephritis (LN) patients and non-LN patients.

CONCLUSIONThe TMEM39A polymorphisms at 1880G/A and 2442T/G, but not at 2456 A/T gene, may be associated with the susceptibility to SLE in Chinese Han population. The genotype or allele frequencies of the 3 SNPs have no effect on the incidence of lupus nephritis.

Adult ; Alleles ; Asian Continental Ancestry Group ; genetics ; Case-Control Studies ; Female ; Gene Frequency ; Genotype ; Humans ; Lupus Erythematosus, Systemic ; genetics ; Male ; Membrane Proteins ; genetics ; Middle Aged ; Polymorphism, Single Nucleotide ; Young Adult

OBJECTIVETo evaluate the analgesic effect of Corydalis yanhusuo on trigeminal neuropathic pain.in a rat model.

METHODRat model of trigeminal neuralgia pain were established by inducing chronic constriction injury (CCI) of the infraorbital branch of the trigeminal nerve (ION). The effect of Corydalis yanhusuo, a traditional Chinese medicine, in ameliorating the pain was tested. Western blotting was performed to investigate the change of cannabinoid CB1 receptors in the Vc the injury of the infraorbital branch of the trigeminal nerve (ION-CCI). CB1 receptor antagonist AM 251 was applied to observe its effect on the analgesic effect of Yanhusuo.

RESULTAdministration of dl-THP (2 mg/kg) intraperitoneally increased the response threshold and the cut-off threshold to the mechanical stimulation in ION-CCI rat models. ION-CCI induced an upregulation of cannabinoid CB1 receptors within the ipsilateral of Vc. The effect of Yanhusuo was antagonized by the application of AM 251.

CONCLUSIONThe analgesic effect of Yanhusuo involves the participation of CB1 receptors, suggesting that Yanhusuo may offer a useful therapeutic approach for trigeminal neuropathic pain.

Analgesics, Non-Narcotic ; therapeutic use ; Animals ; Corydalis ; chemistry ; Drugs, Chinese Herbal ; therapeutic use ; Male ; Phytotherapy ; Rats ; Rats, Sprague-Dawley ; Receptor, Cannabinoid, CB1 ; drug effects ; Trigeminal Nerve Diseases ; drug therapy ; etiology

OBJECTIVETo prepare tanshinone microemulsion (Tan-ME) and investigate its properities and the absorption character in rat intestine in situ.

METHODThe microemulsions were prepared and characterized using Zetapals Zeta potential/particle size analysis and atomic force microscope. A HPLC method for determination of tanshinone IIA in the intestinal flux was established. RESUILT: The Tan-ME was fine droplet with an average droplet size of (32.25 +/- 6.59) nm. The results of Tan-ME absorption in small intestinal indicated that Tan-ME could improve the absorption of tanshinone IIA in rat small intestine, but the absorption coefficient of Tan-ME, Ka was influenced by the ratio of water-phase in Tan-ME.

CONCLUSIONTan-ME could improve the absorption of tanshinone IIA in rat small intestine. Thus bioavailability of tanshinone was improved.

Animals ; Diterpenes, Abietane ; Emulsions ; Intestinal Absorption ; Intestine, Small ; metabolism ; Male ; Particle Size ; Phenanthrenes ; administration & dosage ; isolation & purification ; pharmacokinetics ; Plants, Medicinal ; chemistry ; Rats ; Rats, Sprague-Dawley ; Salvia miltiorrhiza ; chemistry ; Technology, Pharmaceutical ; methods