OBJECTIVE To understand the drug resistance genes in Klebsiella pneumoniae (KPN) isolate resistance to 18 kinds of antibacterials which included imipenem and meropenem. METHODS We detected 36 kinds of drug resistance genes for the strain of KPN by PCR method,included the beta-lactamases genes,blaTEM,blaSHV,blaLEN,blaOKP,(blaCTX-M-1,2 and 9) groups,(blaOXA-1,2 and 10) groups,CARB,PER,VEB and GES; the genes of metallo-beta-lactamases genes,IMP,VIM and KPC; the AmpC genes,DHA,ACT,MOX and LAT; the aminoglycosides resistant genes,aac(3)-Ⅰ,aac(3)-Ⅱ,aac(6')-Ⅰb,aac(6')-Ⅱ,ant(2″)-Ⅰ and ant(3″)-Ⅰ; the quinolones resistant gene qnr; the TMP resistant genes,dfrA1 and dfrA17; the disinfectant-sulfanilamide resistantgene,qacE△1-sul1;the integron genes,intⅠ-1,intⅠ-2 and intⅠ-3; and the transposon genes,tnpA and merA.RESULTS We found 9 kinds of drug resistance genes in this KPN isolate. They were the beta-lactamases genes,blaTEM and blaSHV; the metallo-beta-lactamasesgene blaKPC-2; the aminoglycosides resistant genes,aac(3)-Ⅱ and ant(3″)-Ⅰ; the quinolones resistant gene qnr; the TMP resistant gene dfrA17; the disinfectant-sulfanilamide resistant gene qacE△1-sul1 and the integron genes intⅠ-1. CONCLUSIONS We discovere multiple drug resistant genes (some in the chromosome,some are plasmid-mediated) in this isolate. We also find the infrequent plasmid-mediated drug resistant gene blaKPC-2. We think it's concerned with the pan-resistant and the multi-drug resistant genes in this KPN,and we must pay highly attention to this isolate in clinic.