Objective To investigate the levels of monocyte chemoattractant protein 4 (MCP-4), macrophage derived chemokine (MDC) and cysteinyl leukotrienes (CysLTs) in serum of children with mycoplasma pneumonia (MP). Methods Serum levels of MCP-4, MDC and CysLTs measured by enzyme linked immunosorbent assay were analyzed in 60 children with MP including 36 children with wheezing (MP wheezing group) and 24 children without wheezing (MP non-wheezing group), 30 children with pneumonia but not infected with mycoplasma pneumonia (NMP group), 35 children with acute asthma exacerbation (asthma group), and 25 health children (control group). Results Serum levels of MCP-4, CysLTs and MDC were found markedly elevated in asthma group. The serum levels of MCP-4 and MDC showed signiifcant difference between each of the groups (all P<0.01) except that between non-wheezing group and NMP group (P>0.05). As for serum level of CysLTs, no signiifcant differences were found between asthma group and MP wheezing group, NMP group and MP-non-wheezing group (all P>0.05). The serum levels of MCP-4, MCD and CysLTs in children with MP were positively correlated with one another. Conclusions MCP-4, MDC and CysLTs play important roles in pathogenesis of MP and are the major causes of wheezing in MP.

Background Clinical work found that triamcinolone acetonide (TA)bleeding during vitrectomy in proliferative diabetic retinopathy (PDR),but its mechanism is not clear.Objective This study was to explore the anastalsis of TA in vitrectomy for PDR.Methods A prospective study was performed.Twelve eyes of 12 patients who received vitrectomy combined with the intraocular use of TA for PDR were in cluded in Tianjin Medical University General Hospital from 2011 to 2014 and served as TA group.Thirty-two eyes of 32 patients who underwent vitrectomy for epimacular membrane or macular hole were enrolled as control group.The vitreous specimens of 0.6 ～0.8 ml was collected during the surgery.The concentrations of urokinase plasminogen activator (u-PA),tissue plasminogen activator (t-PA) and plasminogen activator inhibitors 1 (PAI-1) in vatreous were measured by ELISA.Results The mean contents u-PA,t-PA and PAI-1 in the vatreous were 25.45,127.44 and 0.42 ng/ml respectively in the TA group,and those the mean contents in the control group were 22.94,142.37 and 0.27 ng/ml respectively,shouwing a significant difference between the TA group and the control group (Z=-2.268,P＜0.05).NO significant difference was found in vitreous t-PA and PAI-1 between TA and control groups (Z =-0.092,-1.847,both at P＞0.05).Conclusions Vitreous u-PA content is increased in PDR eyes,which is more likely to lead bleeding.Anastalsis of TA during vitrectomy for PDR may be relatived to decreasing vitreous t-PA and u-PA contents as well as increasing PAI-1 contents.