Objective To understand the changes of the coverage of medical insurance and the utilization of medical services from 2000 to 2009.Methods The data on medical insurance and utilization of medical services were obtained from China Nutrition Panel Survey conducted by University of North Carolina,Chapel Hill.The trend of medical insurance and use of preventative medical services were examined.And a Logistic regression model was used to estimate the relationship between some social economic status and the enrollment of medical insurance and use of medical services.Results The coverage of medical insurance expanded dramatically from 20％ in 2000 to 89％ in 2009.The rural population experienced higher expansion.The gap between rural and urban populations in terms of insurance coverage was closed.The use of preventive services remained low in both rural and urban populations,with 3.72％ and 4.62％ respectively.Conclusions Both coverage of medical insurance and use of prevention medical services imoroved from 2000 to 2009,which has provided solid foundation for further reforms.

Objective Effects of methylprednisolone on observation of intramedullary spinal cord tumor treatment.Methods Through the 9 cases of intramedullary spinal cord tumors in patients after adjuvant therapy of methylprednisolone clinical observation and analysis.Results 9 cases of ostoperative patients after treatment with methylprednisolone,his condition to resume soon,but the response to 5 cases of side effects,as symptomatic treatment can be back to normal.Conclusions Application of adjuvant therapy of methylprednisolone after spinal marrow tumor effects although good,it should be noted that the occurrence of side effects,and do:the right of dispensing,close observation and timely manner.

Objective To study the effect of different processing methods on paeoniflorin content and to provide a basis for stabilizing the processing technology of white peony root.Methods RP-HPLC method was used to determine the content of paeoniflorin on Agilent Eclipse XDB-C18(150 mm?4.6 mm,5 ?m),with mobile phase of acetonitrile-0.1% phosphoric acid(14∶86),flow rate of 1.0 mL/min,column temperature of 25 ℃,and detective wavelength of 230 nm.Results The average recovery rate was 99.195%,RSD=0.8%(n=6).The content of paeoniflorin was 0.55%,2.70%,3.40%,3.25%,respectively.Conclusion In the processing of white peony root,heating,water immersion and sulfur smoked have greater effect of paeoniflorin content in white peony root.

Objective:To investigate if injury-related myofibroblast induction could occur in bronchial epithelial cells and the role of TGF-?_1(rhTGF-?_1) in epithelial-myofibroblast transdifferentiation. Methods:Cultured bronchial epithelial cells, 16HBE-14o, were exposed to poly-L-arginine(PLA). Using a immunohistochemical method, ?-smooth muscle actin (?-SMA) expression was detected in those cells after being treated with PLA . Relative activity of chloramphenicol acetyltransferase(CAT) was assessed in 16HBE-14o cells, which were transiently transfected with VSMP8 plasmid containing ?-SMA promoter linked to CAT gene, at different time points after rhTGF-?_1(10 ?g/L) treatment. Results:①?-SMA positive, spindle-shaped cells emerged among the regenerated 16HBE-14o epithelial cells following damage. The number of ?-SMA positive 16HBE-14o cells increased and cell repair was delayed under rhTGF-?_1 stimulation.② CAT activity increased significantly in VSMP8 plasmid transfected 16HBE-14o cells 24 h after TGF-?_1 stimulation. (P

Objective To investigate the use of sodium hyaluronate and the preservation of epidural fat in the prevention of epidural scar adhesion after microendoscopic discectomy (MED). Methods A total of 300 patients receiving MED from July 2000 to October 2001 were randomly divided into 2 groups: patients in group A were given sodium hyaluronate with the preservation of epidural fat, while those in group B were on the contrary. Results The mean follow-up duration was 42 months in the group A and 44 months in the group B, respectively. The rate of excellent or good results was 98.6% in the group A (144/146), while 93.8% in the group B (135/144) ( ? 2=4.731, P =0.030). Postoperatively, the pain reappeared in 4 cases in the group A and in 6 cases in the group B, respectively, in which a re-operation was required. Re-operations found the epidural scar adhesion was grade 0 (3 cases) and grade 1 (1 case) in the group A, and grade 2 (2 cases) and grade 3 (4 cases) in the group B ( P =0.005). Conclusions Use of sodium hyaluronate with the preservation of epidural fat can improve the efficacy of MED and effectively prevent epidural scar adhesion.

2 weeks ) were screened by BAEP. Results Hearing-ability of 11 children(11/23,47.8%) was abnormal,one month later hearing-ability of 2 children was abnormal, and there months later hearing-ability of only 1 child was abnormal. Conclusion Hyperbilirubinemia may cause hearing-ability damage in neonates and BAEP is one of effective audiometric means.

AIM:To explore the cataract suspensory ligament rupture and artificial lens implantation suture fixation into capsular bag without capsular tension ring(CTR).METHODS:We reviewed 20 cases of 20 cataract suspensory ligament rupture without CTR intraocular lens (IOL) implantation fixation in our department from Jan.2012 to Dec.2016.The needle crossed into ocular ciliary sulcus, in the equator of the eye ball which suspensory ligament rupture from, then the needle crossed out 1.5mm away from the angle of sclera.Sutures fixed on the IOL, then the artificial lens implantation in the pouch, carried out in accordance with the Z type suture, or to the beforehand prepared triangle scleral flap.The visual acuity, intraocular pressure, the anterior chamber and the IOL position were measured after operations.RESULTS:All of the postoperative visual acuity improved different level.The postoperative best corrected visual acuity(BCVA) was ≥0.8 in 4 eyes(20%), 0.5-0.6 in 7 eyes(35%), 0.3-0.4 in 8 eyes(40%), 0.1 in 1 eye(5%) because of the glaucoma optic atrophy.There were 12 cases with mild corneal endothelium edema, 4 cases exudation membrane in the pupil area, 2 cases hyphema, all of which recovered after treatment.There were 2 eyes with vitreous prolapse in the pupil, 1 case appeared mild IOL center deviation and no special treatment for the vision did not be involved.Followed up for 6mo, displaced stitches or artificial lens shift did not occur.CONCLUSION:Without CTR, the IOL implantation and suture fixation in capsular bag during cataract surgery is a surgical method for basic-level hospitals.

Objective To study the safety and efficacy of selective head cooling (SHC) with mild systemic hypotherrnia in neonates with HIE. Methods Fifty-four term infants with severe neonatal HIE were randomly assigned to the head cooling group (n=27) and control group (n=27). Forty-one infants in 96 h after admission were eligible for the study(SHC group n=21, control group n=20). In SHC group, the naso-pharyngeal temperature was maintained at (34.0±0.2) ℃ and rectal temperature maintained at 34～35 ℃ for 72 h, then rewarmed spontaneously. In control group, normal rectal temperature was maintained. During the period of the study, the infants of two groups were monitored on nasopharyngeal temperature, heart rate, respiratory rate,transcutaneous arterial oxygen saturation and blood pressure. Primary adverse effects inclu-ding severe arrhythmia, venous thrombosis or hemorrhage and severe hypotension were observed. The efficacy indicators including rate of death and severe disability, exercise and cognition development index were as-sessed. Results Severe arrhythmia, hypotension and renal failure were not found in both groups. Follow-up was conducted until postnatal 18 months and was not available in 6 babies (3 in SHC group and 3 in control group respectively). Death and severe disability occurred in 4 of 18 infants (22.2%)in SHC group and in 9 of 17 infants(52.9% ) in the control group respectively (P<0.05). Conclusion SHC for 72 h with mild systemic hypothermia in neonates with HIE is safe and effective. The therapy could reduce the risk of disabili-ty and handicap significantly.

Long non-coding RNAs (lncRNA) play important roles in many biological processes.However,compared with other types of RNAs,limited research has been conducted on lncRNA,mostly concerning its relationship with tumor,and there are much fewer studies on its relationship with atherosclerosis.In this article,we summarized the latest research findings in this field,with an emphasis on the importance of IncRNA in atherosclerosis.The value of lncRNA in targeted therapy of atherosclerotic disorders is also discussed.

Objective: To observe the clinical efficacy of electroacupuncture in treating spastic paralysis following cerebral infarction. Methods: Sixty patients with spastic paralysis after cerebral infarction were randomly allocated into control group and treatment group, 30 cases each. The control group was treated with conventional acupuncture and the treatment group was treated with conventional acupuncture plus electroacupuncture according to the principle of antagonistic acupuncture. Both groups were given routine drugs and scalp acupuncture treatment. Results: Statistical analysis showed significant differences in NFI score and clinical curative effect score between pretreatment and posttreatment in the treatment and control groups and between the treatment and control groups. Conclusion: Both electroacupuncture and conventional acupuncture have clinical curative effect on spastic paralysis following cerebral infarction, but the curative effect of electroacupuncture is significantly superior to that of conventional acupuncture.