Objective To investigate the biochemical differences between the cartilage of the residual ear of microtia patient and normal person. Methods Seven cartilage specimens from 7 about 10-years-old microtia patient's residual ears and 7 normal ears from cadaver of the same age were measured by electrophoretic mobility shift assay and spectrophotometer for collagen glycoaminoglycan (GAG), chondroitin sulfate (Chs), hyaluronan (HA) and keratin sulfate (KS) .Results Biochemical analysis showed that no significant difference were found in the collagen contents of the two groups. The microtia cartilage had more GAG (49.00?25.60) ?g/mg than that of the normal (28.25?4.80) ?g/mg. The composition of GAG were different between two groups: microtia group (HA 38.96+4.97 %, Chs 29.02 %?4.12 %, KS 32.16 %?7.41) % and normal group (HA 32.94 %?3.24 %, Chs 33.10 %?2.61%, KS 33.96 %?1.66 %). There were differences in HA and Chs relative contents, but not in KS between the two groups. Conclusions There is no difference in the collagen content between normal and microtia ear cartilage. But the latter has more GAG than the former. As for the constitution of GAG, microtia cartilage has a higher percentage of HA, lower percentage of Chs, and no significant difference in KS.

Objective To summarize the ruptured types and surgical treatment experience for rupture of aortic sinus aneurysm. Methods Nineteen cases with aneurysm of aortic sinus were retrospectively analyzed. There were 10 cases with ventricular septal defect,6 cases with aortic valvular incompetence,6cases with other diseases,2 cases complicated by infective endocarditis. All cases underwent operational treatment. Results There were 15 cases of right aneurysm of aortic sinus,including 12 cases among them ruptured into right ventricle,3 cases rupture was penetrated into right atrium.There were 3 cases of null aneurysm of aortic sinus,including 1 case among them ruptured into right ventricle,2 cases ruptured into right atrium. There was 1 case of left aneurysm of aortic sinus, which ruptured into left ventricle. No operative death occurred in all, 1 case with aortic regurgitation light to moderate,and all cases were recovered.Conclusions The right aneurysm of aortic sinus is the most common types,and always rupture into right ventricle. It is important that aneurysm of aortic sinus should be operated as early as possible in order to avoid losing the chance of operation, particular in the patients complicated by infective endocarditis.

Objective　To study the causes of failure to IOL implantation during cataract surgery.Methods　We reviewed 540 eyes in which 23 eyes were failed to implant IOL during cataract surgery.Results　Detachment of suspended zonular ligament, rupture of posterior capsule and vitreous prolapse are the main factors influencing IOL implantation during cataract surgery.Conclusion　Improvement of surgical technique and reduction of complications are the key points for the IOL implantation successfully during cataract surgery.

Objective To investigate the effect of EGB on SOD, MDA of ventilator-induced lung injury in rats and its possible mechanisms. Methods Thirty male SD rats were randomly divided into 3 groups: control group (C group), high tidal ventilation group (H group) and EGB group (E group). The setting mechanical ventilation was VT=30 mL/kg, RR=40/min, I/E=1/3, PEEP=0 cmH2 O and FiO2=21%. The broncho-alveolar lavage fluid (BLAF) and serum were obtained for determination of the levels of SOD and MDA at the end of 4 h mechanical ventilation. The Lungs were removed, and the wet-to-dry weight ratio (W/D) and pulmonary pathologic changes were measured. Results As compared with C group, W/D and the levels of MDA were significantly increased in H group, but the levels of SOD were reduced in H group. As compared with H group, W/D and the levels of MDA were significantly decreased in E group, but the levels of SOD were increased in E group. Pulmonary pathologic changes were alleviated in E group comparing with H group. Conclusion EGB injection may have a protective role against hyperoxia and induced pulmonary damage in rats.

OBJECTIVE: To study the situation and the related factors of ADR occurred in our hospital and to provide references for rational use of drug. METHODS: A total of 246 ADR case reports collected in our hospital from Nov. 2007 to Oct. 2008 were analyzed statistically. RESULTS: Of the 246 ADR cases, 120 kinds of drugs were involved. 64.63% of ADR were induced by intravenous drip infusion; 42.28% were antimicrobial,13.01% were drugs for central nervous system, 10.57% were for circulation system, 7.32% were TCM preparation; ADR-inducing TCM injection accounted for 50.00% in TCM preparations. The main clinical manifestation represented as lesion of skin and its appendants (51.63%). 11 severe cases accounted for 4.47% and 1 new cases only 0.41%. CONCLUSION: ADR may be caused by many factors. The most attention should be paid to monitoring ADR in the clinic so as reduce or avoid occurrence of ADR.

Objective To investigate the possibility of using poly-hydroxybutyate-hydroxyhexate p(3HB-co-3HH)), a modified extracellular matrix, the third generation of PHA family, as a scaffold for seeding of chondrocytes from the residual ear of microtia patients to generate tissue engineered cartilage in athymus mice. Methods The residual ear cartilage from eight 7-years-old microtia patients was enzymatically dissolved by collagenase, and chondrocytes were harvested and seeded into foams of PHB-PHH. After incubation for 1 week in vitro, chondrocyte-polymer constructs were implanted subcutaneously into 8 athymus mice. A control groups was established by subcutaneous implantation of PHB-PHH foams alone. One athymus mice were killed at 4 weeks postoperatively, and 6 were sacrificed at 8 weeks. The specimens were dissected and examined macroscopically and histologically. Results Specimens harvested from chondrocyte-polymer constructs subjected to gross morphologic and histology analysis demonstrated new cartilage formation, and those from control groups showed no cartilage formation. The one of 4 weeks still had some remains of the scaffold with nodules of neocartilage. After 8 weeks, all the 6 mice had neocartilage formed almost the same as natural. The PHB-PHH scaffold were totally absorbed. Conclusion These findings demonstrate that the foam of PHB-PHH is not only a good "matrix" for cartilage tissue engineering, but also optimal scaffold for the seeding of chondrocytes from the residual ear to generate new cartilage that would be useful in plastic and reconstructive surgery. Chondrocytes from the residual ear of microtia patients are good candidates for generation of tissue engineered cartilage.

Objective To invstigate the diagnostic value of the detection of telomerase activity in the specimen obtained from CT guided percutaneous transthoracic needle biopsy of lung nodules.Methods The techniques of TRAP silver staining were performed to detect telomerase activity in 43 cases. Results 43 mass cases were studied includirg 32 proved to be primary lung cancer, and 11 benign lesions cytologically and clinically. Telomerase activity was detected as 28(87.5%) of 32 lung carcinomas, whereas in benign lung lesions was 1(9.1%) in 11 cases.Conclusions Detection of telomerase activity in the specimen of lung nodules by CT guided percutaneous transthoracic needle biopsy may be an effective method in the diagnosis of lung cancer and also as tumor marker.

Objective To assess the associations of anti-Smith antibodies with clinical manifestations and disease activity in children with systemic lupus erythematosus (SLE).Methods According to SLE disease activity index (SLEDAI) score,72 children with SLE were divided into the active group and inactive group.An immunoblotting method was used to detect serum anti-Smith antibodies in these subjects.Chi-square test was conducted to assess the associations of anti-Smith antibodies with clinical manifestations and disease activity in these patients.Results Of these patients,28 (38.9％) were assigned into the inactive group,and 44 (61.1％) to the active group.Anti-Smith antibodies were detected in 17 (23.6％) patients,but not in the other 55 (76.4％) patients.Elevated incidence rate of kidney injury was observed in anti-Smith antibody-positive patients compared with anti-Smith antibody-negative patients (70.6％ (12/17) vs.41.8％ (23/55),P ＜ 0.05).Meanwhile,the positivity rate of anti-Smith antibodies was 31.8％ (14/44) in the active group,significantly higher than that in the inactive group (10.7％,3/28,P ＜ 0.05).Conclusions Anti-Smith antibodies are not only an important indicator for the diagnosis of SLE,but also a risk factor for disease exacerbation and kidney injury in children with SLE.

Objective To observe the effects of low frequency electrical stimulation (LFES) on the proliferation of endogenous brain neural stem cells (NSCs) and on the expression of basic fibroblast growth factor (bFGF)and epidermal growth factor (EGF) in rats with acute cerebral infarction; to explore the therapeutic mechanism of LFES in improving neural function. Methods Fifty-four rats were randomly divided into a LFES group, a placebo stimulation group and a sham-operated group. Each group was further divided into 3rd day, 7th day and 14th day subgroups, with 6 rats in each subgroup. An acute cerebral infarction model was induced in the rats of the LFES and placebo stimulation groups by middle cerebral artery occlusion (MCAO). Three days after the operation, rats in the LFES group began LFES treatment (frequency 30 Hz, pulse width 250 μs, current intensity 3 mA, 10 min/d) ,while the placebo stimulation group was treated identically but without electricity. The rats in the sham-operated group had no special treatment. The expression of nestin positive cells in the subgranular zone of the hippocampus and the subventricular zone was detected by immunohistochemical staining. The expression of bFGF, EGF proteins and mRNA in the ischemic hemisphere was detected by Western blotting and RT-PCR analysis. A screen test was applied to evaluate motor function. Results Nestin-positive cells in the subgranular and subventricular zones of rats in the LFES group increased significantly more than in the placebo stimulation group at the 7th and 14th day. The expression of bFGF, EGF proteins and mRNA in the ischemic hemisphere was up-regulated compared to the placebo stimulation group at the 7th and 14th day. At the 14th day a difference in motor function was observed in rats in the LFES group compared with the placebo stimulation group. Conclusion LFES can promote the proliferation of endogenous brain NSCs and the expression of bFGF and EGF in rats with acute cerebral infarction. It can also improve motor function and enhance neural plasticity in the brain.

Objective To compare the clinical efficacies between warm needling and Ibuprofen sustained release capsules (a nonsteroidal anti-inflammatory drug, NSAID) in treating patients with dysmenorrhea in adenomyosis. MethodSixty-five patients with dysmenorrhea induced by adenomyosis were randomized into a treatment group of 33 cases and a control group of 32 cases. The control group was intervened by oral administration of Ibuprofen sustained release capsules, while the treatment group was intervened by warm needling.The intervention lasted 3 menstrual cycles and a 3-month follow-up was studied. The Visual Analogue Scale (VAS), dysmenorrhea symptoms scores and clinical efficacy were compared between the two groups.ResultThe VAS scores after the intervention and inthe first and second months of the follow-up study were significantly different from the pre-treatment score in the two groups (P<0.01); the VAS score of the 3-month follow-up was significantly different from the score before the intervention in the treatment group (P<0.01). There were significant differences in comparing the VAS score after the intervention and in the follow-up study between the two groups (P<0.01), and the treatment group was superior to the control group. The dysmenorrhea symptoms scoresdeclined significantly after the intervention and in the first and second months of the follow-up study in both groups (P<0.01); the dysmenorrhea symptoms score of the 3-month follow-up study decreased in the treatment group and was significantly different from the pre-treatment score (P<0.01). There were significant differences in comparing the dysmenorrhea symptoms scores in the second and third months of the follow-up study between the two groups (P<0.01). The total effective rate was 93.9% in the treatment group, significantly better than 62.5% in the control group (P<0.01).ConclusionWarm needling is effective in easing pain and improving the symptoms of dysmenorrhea in adenomyosis, and can produce a consistent efficacy after the termination of thetreatment; it's superior to NSAIDs in comparing both short-term and long-term treatment efficacies.