Objective To study the levels of interleukin(IL)-2 ,interferon-γ(IFN-γ) and IL-10 in peripheral blood and the effec-tive concentration of lactate dehydrogenase(LDH) in serum of patients with acute leukemia .Methods 65 patients with acute leuke-mia were divided into non-remission group(n=35) and remission group(n=30) .50 healthy volunteers were used as control group . The levels of IL-2 ,IFN-γ ,IL-10 in peripheral blood were measured by enzyme linked immunosorbent assay(ELISA) .The effective concentration of LDH was measured by automatic biochemical analyzer .Results The levels of IL-2 ,IFN-γin non-remission group were significantly decreased compared with control group and remission group(P<0 .05) ,but the level of IL-10 in non-remission group was significantly increased compared with control group and remission group(P<0 .05) .The effective concentration of LDH in non-remission group was significantly higher than that in control group or remission group(P<0 .05) .Conclusion The levels of IL-2 ,IFN-γand IL-10 in peripheral blood and the effective concentration of LDH in serum of patients with acute leukemia have changed .Clinical detection of IL-2 ,IFN-γ,IL-10 and LDH may contribute to the early diagnosis of patients with acute leukemia .

Objective To investigate the diagnostic threshold value and the influencing factors of high‐sensitivity troponin I measurement in the diagnosis of patients with acute myocardial infarction .Methods Collecting 9 236 patients who are the first time hs‐cTnI testing in the internal emergency department of our hospital(including 9 099 patients with non‐AMI and 137 patients with AMI) as participants ,then dividing non‐AMI patients into elder group(>60 years old) and younger group(≤60 years old) ,hs‐cTnI concentrations were compared between the elder and the younger group with different gender .The optimal threshold value of hs‐cT‐nI for acute myocardial infarction was determined by receiver operating characteristic(ROC) curve of AMI patients with different age and gender .Results The hs‐cTnI concentrations of non‐AMI patients in the elder male group were 39 .21 ng/L [(37 .48-40 .93)ng/L] ,which was higher than those of non‐AMI patients in the younger male group 22 .38 ng/L[(21 .16-23 .6)ng/L] and the difference was statistically significant(P<0 .05) ,the same result was seen in the elder female group and the younger female group .The total male group was 31 .26 ng/L [(30 .17 -32 .34)ng/L] ,it was higher than the total female group 26 .8 ng/L [(25 .73-27 .86)ng/L] .According to the ROC curve ,the optimal threshold value for AMI diagnosis was 45 ng/L in total AMI group and 135 ng/L in the elder male group and 45 ng/L in the elder female group ,which was different with the cut off value(120 ng/L) .Conclusion the hs‐cTnI levels were related with age and gender ,it is very important for us to consider these factors when we make the optimal threshold value with our own data .

Objective: To study the protective effect of ? carotene in anoxia injured cardiomyocytes. [WT5FZ]Methods: The cultured rat neonatal cardiomyocytes were subjected to 2h anoxia and the changes of lactate dehydrogenase(LDH) activity, malondialdehyde (MDA), oxygen free radical (OFR) contents and superoxide dismutase (SOD) activity were detected. The effects of pre treatment with ? carotene were then investigated. Results: The LDH, MDA, OFR and SOD of cardiomycytes were changed significantly after 2h anoxia (P

Objective To observe the clinical change of cytokines expression in peripheral blood in patients with allergic asthma and its significance .Methods Forty patients with allergic asthma and thirty healthy controls were enrolled .The concentrations of IL‐17 ,IL‐22 and IL‐10 in peripheral blood were measured by ELISA ;the serum IgE levels were measured by electrochemical lumi‐nescence method ;The eosinophil proportion was detected by blood cell analyzer ;the IL‐17 ,IL‐22 ,IL‐10 and the severity of asthma were conducted Pearson correlation analysis .Results The level of serum IgE and eosinophil proportion was significantly higher in patients with allergic asthma compared with the controls (P<0 .05);compared with the control group ,the concentration of IL‐17 and IL‐22 in peripheral blood of patients with allergic asthma were increased ,but the expression of IL‐10 were decreased (P<0 .05);the level of IL‐17 and IL‐22 were positively correlated with severity of allergic asthma (P<0 .01) ,but the level of IL‐10 was negatively correlated with severity of allergic asthma (P<0 .05) .Conclusion The expression of IL‐17 ,IL‐22 and IL‐10 play an im‐portant role in the pathogenetic mechanism of allergic asthma ,and a dynamic monitoring could be helpful for the diagnoses and treatment of allergic asthma .

On the basis of the investigation of the pathogenesis of cervical spondylotic radiculopathy, this paper studies the mechanisms of spinal micro-adjustment manipulations in recovering and improving cervical dynamic and stationary balance from the perspective of biomechanics.

Objective To investigate the relationship between serum nitric oxide (NO) and bone metabolism in streptozotocin induced early diabetic (STZ DM) rats. Methods Twenty SD rats were divided into 2 groups, 12 STZ DM rats and 8 controls. Fasting blood glucose, HbA 1c , serum insulin, bone mineral density (BMD) (whole body, lumbar and femoral bone), bone metabolic parameters 〔such as serum calcium, vitamin D 3, parathyroid hormone (PTH), calcitonin, osteocalcin, and urinary pyridinoline/creatinine〕, as well as serum NO were measured. Results Compared with the controls, serum NO in STZ DM group significantly elevated 〔(51.3?11.9 vs 38.1?12.0)?mol/L, P

Objective To analyze the CT features of intestinal tumor obstruction, and explore its CT value.Methods CT manifestation and clinical materials of intestinal tumor obstruction proved by surgical findings or endoscopy biopsy in 35 cases were analyzed restrospectively, and compared with the results of surgery-pathology and endoscopy. Results CT findings were consistence with the results of surgery-pathology and endoscopy in 33 of 35 cases. The CT diagnostic accuracy of intestinal obstruction was 100%. And the accuracy of the cause was 94%, including colon carcinoma in 25, lyphoma in 5, gastrointestinal stromal tumor in 2, and lipoma in 3.Conclusion CT has unique advantage in examining intestinal tumor obstruction, not only for definiting the existence of the obstruction, but also locating the site of obstruction diagnosing the cause and chosing the appropriate treatment.

Objective To investigate the diagnostic Value of multi-slice spiral CT 3-Dimensional reconstruction in intestinal obstruction.Methods 28 Patients with malignant lesions of colon underwent volume scanning using multislice helical CT.Four types of reconstruction included multiple planner reconstruction(MPR),CT virtual colonoscopy(CTVC),shaded surface display(SSD)and raysum.The results were compared with those of colonoscopy and patholog.Results CT diagnostic accurate rate was 100%,and the accurate rate of the cause was 96% in intestinal obstruction,including primary or secondary neoplasms(18/28),abhesions(2/28),bowel torsion(2/28)and external or internal hernias(3/28) and gallstone ileus(3/28).Conclusion There were advantages and disadvantages in diagnosis of mechanical intestinal obstruction with MPR,CTVC,SSD and Raysum respectively.The combined use of the four post-processing technigues can be more helpful to precise localizing and qualitative diagnosis of lesions.MPR should be the optimal post-processing technigues for the diagnosis of intestinal obstruction

Objective To investigate CT in combination with MRI in diagnosing and differential diagnosing chondrosarcoma.Methods CT and MRI findings pathologically in 19 cases of chondrosarcoma proved were analyzed retrospectively.Results On CT,the tumors appeared as bony destruction in 15 cases,soft tissue masses in 14 cases,and with multiple calcifications in different shape.Postcontrast CT scan demonstrated peripheral and septal enhancement in 2 cases,and inhomogeneous enhancement in 3 cases.On MRI,the chondrosarcomas were hypointense or isointense on T1WI,heterogeneous hyperintense on T2WI.Postcontrast MRI demonstrated peripheral and septal enhancement with wreath-or honeycomb-like appearance in 9 cases,heterogeneous enhancement in 2 cases,obvious enhancement in 2 cases.On fat-suppression T2WI,the boundary and size of lesions were demonstrated clearly.The phylloid and cauliflower-like shape of tumors were displayed better on sagital and coronal position.Conclusion CT and MRI are both important in diagnosis and differetial diagnosis of chondrosarcoma,the combination of the two methods may improve the diagnosing accuracy of this tumor and minimize misdiagnosis.

(0.05)). (Conclusions) PFC and serum TNF-? ,NO could be early observation indicators of acute rejection in minipig pancreas transplatation,and should have important significance in the diagnosis of acute rejection.