Standardized management for medical equipment department of the second-class traditional Chinese medical hospital is of great importance to enhance working and economic efficiencies. When standardized management performed, it's suggested that related organization, regulation as well as maintenance and file management be involved in.

Objective:To study the influence of hepatocellular carcinoma cells microenvironment on the biophysical characteristics of mature dendritic cells(mDCs)and explore the immune escape mechanism of tumor cell from interdiscipline viewpoint.Methods:The monocytes were then cultured in RPMI1640/10% FBS supplemented with 100 ng/ml rhGM-CSF and 100 ng/ml rhIL-4 for 7 days to develop into immature DCs(imDCs).mDCs was induced by addition of 20 ng/ml rhTNF-? to imDCs for another 3 days of culture.Acquired mDCs were co-cultured with human HCCs for 48 hours in Transwell.The treated cells were investigated the viscoelastic property,membrane fluidity and transendothelial migration by micropipette,fluorescence polarization and Transwell,respectively,untreated cells as control.Results:Compared with untreated DCs,the DCs co-cultured with HCC appeared decreased viscoelastic property,membrane fluidity and impaired transendothelial migration.Conclusion:HCCs can impaire the biophysical characteristics of mDCs leading to their deteriorated transendothelial migration.This might be one aspect of escape from immune surveillance of tumor.It is significant for further understanding the immune escape mechanism of tumor.

Diagnosis, Differential ; Humans ; Neoplasms ; classification ; genetics ; pathology ; Survival Analysis ; Terminology as Topic ; World Health Organization

In this paper, we designed an oxygen saturation, heart rate, respiration rate monitoring system based on smartphone of android operating system, physiological signal acquired by MSP430 microcontroller and transmitted by Bluetooth module.
Cell Phone ; Equipment Design ; Heart Rate ; Humans ; Monitoring, Physiologic ; instrumentation ; Oxygen ; Respiratory Rate ; Software ; Wireless Technology

Antibodies, Monoclonal ; administration & dosage ; Antigens, CD20 ; drug effects ; metabolism ; Antineoplastic Agents ; administration & dosage ; Drug Delivery Systems ; methods ; Fusion Proteins, bcr-abl ; drug effects ; metabolism ; Genetic Therapy ; Humans ; Neoplasms ; metabolism ; pathology ; therapy ; Receptor, ErbB-2 ; drug effects ; metabolism

Objective To study the influence of microenvironment created by dissoluble cytokines derived from hepatocellular carcinoma cells (HCCs) on the functional status of mitochondria in dendritic cells (DCs) in order to investigate the mechanisms of tumor immune escape.Methods CD14+ monocytes,which were isolated with immune magnetic beads from fresh peripheral blood of healthy human,were then cultured in RPMI1640/10% FBS supplemented with 100 ng/ml rhGM-CSF and 100 ng/ml rhIL-4 for 7 d to develop into immature DCs (imDCs).Maturation was induced by addition of 20 ng/ml rhTNF-? to imDCs for another 3 d of culture.Acquired imDCs and mDCs were respectively co-cultured with human HCCs for 48 h in Transwell chamber.These treated cells were investigated for mitochondrial membrane potential,enzyme activity and intracellular Ca2+ concentration ([Ca2+]in) in the cytoplasma by immune fluorescence and MTT assay.Those untreated cells served as control.Results After co-cultured with HCCs,flow cytometry showed that the mitochondrial membrane potentials of imDCs and mDCs were respectively decreased from (659.991?17.052)and(473.741?11.676)to(482.681?7.935)and(407.189?5.051)(P

Objective To study the influence of microenvironment created by dissoluble cytokines derived from hepatocellular carcinoma cells (HCCs) on the expression of IL-12 of dendritic cells (DCs) at different differentiating stage. Methods The CD14+ monocytes,isolated with immune magnetic beads from fresh peripheral blood of healthy human,were cultured in RPMI 1640/10% FBS supplemented with 100 ng/ml rhGM-CSF and 100 ng/ml rhIL-4 for 7 d to develop into immature DCs (imDCs). Maturation was induced by addition of 20 ng/ml rhTNF-? to imDCs for another 3 days' culture. Acquired imDCs and mDCs were respectively co-cultured with human HCC Bel7402 cells for 48 h in Transwell chamber. The concentrations of IL-10,IL-12,TGF-?1 and VEGF in culture supernatant were investigated by ELISA,imDCs and mDCs cultured alone served as control. Results After co-cultured with Bel7402 cells,IL-12 were decreased from (115.076?8.129) pg/ml to (17.599?1.757) pg/ml in imDCs,and from (258.346?3.609) pg/ml to (6.787?1.123) pg/ml in mDCs (P

Percutaneous transluminal coronary angioplasty (PTCA) is a very effective approach to treat coronary artery disease.However,restenosis after PTCA affects 40% to 60% of patients in the months after an initially successful intervention.Although a number of new techniques and pharmacological approaches have been tried to reduce the rate of restenosis,only a few have shown even preliminary efficacy.Radiation therapy seems to provide an interesting,nonpharmacological approach to prevent the restenosis after PTCA during recent years.The experiments and clinical data of this new approach are reviewed in this paper.

Humans ; Molecular Diagnostic Techniques ; Neoplasms ; pathology ; Pathology, Molecular ; methods ; trends ; Pathology, Surgical ; methods ; trends ; Translational Medical Research

Adenocarcinoma ; classification ; genetics ; pathology ; Adenocarcinoma, Bronchiolo-Alveolar ; genetics ; pathology ; Exons ; Humans ; Lung Neoplasms ; classification ; genetics ; pathology ; Mutation ; Oncogene Proteins, Fusion ; genetics ; Proto-Oncogene Proteins ; genetics ; Proto-Oncogene Proteins p21(ras) ; Receptor, Epidermal Growth Factor ; genetics ; ras Proteins ; genetics