The β-cyclodextrin cross-linked polymer(β-CDCP) was used as adsorbent to pre-concentrate/separate the trace p-nitrophenol and then the determination was carried by UV-Vis spectrophotometry. Under alkaline condition,the adsorption/elution behavior of p-nitrophenol was studied. In 0. 02 mol/L NaOH solution and at room temperature for 30 min,the resin could separate and pre-concentrate the p-nitrophenol effectively. Methanol solution(1:1,V/V) was used as eluent and the β-CDCP could be used repeatedly. The linear range and detection limit was 0.5 -90.0 mg/L and 3. 10 μg/L,respectively. The proposed method has been used to determine the p-nitrophenol in synthesized sample with satisfactory results.

Objective To investigate the effect of health education on the family care for elderly patients.Methods Health education were done during family care for 65 elderly patients.A comparison was done between pre-intervention and post-intervention effect in terms of mastery of disease knowledge,health behavior,curative compliance and emotion management.Result The effect after intervention was significantly better than that before intervention (χ2=28.770,P<0.01).Conclusion During family care for elderly patients,health education is effective for the improvements of mastery of disease knowledge,health behavior,curative compliance,emotion management and ultimately the quality of life.

Objective To observe influence of basic osteoporosis intervention on the 25-hydroxy vitamin D[25(OH)D] levels,low limb muscle strength and its balance function among Chinese elderly people.Methods Cross sectional study and statistics analysis were performed among total 1026 elderly (≥65years) patients from 16 hospitals throughout China.Daily taking amount of dairy products,calcium,vitamin D supplement,sunlight and physical exercise were recorded.Serum 25 (OH) D level was measured.Limb muscle strength and balance tests were performed.Results Daily intake of dairy products was insufficient in more than 42.0％ (430 cases) participating patients.The average sunlight time of participating patients was (1.2 ± 0.8) h per day.The average physical exercise time was (1.2 ± 0.7) h per day.37.7 ％ (387 cases) participating patients took supplemental calcium and vitamin D.36.8％ (278 cases)participating patients took supplemental calcium.The 25 (OH) D level was less than 50 nmol/L in 77.2％ (792 cases) participating patients,while 50～75 nmol/Lin 16.7％(171 cases).48.7％ (474/973),64.6％(624/966)and 63.6％ (614/966) patients were in high fall risk according to the timed up and go test(TUG),chair rising test(CRT) and tandem gait test(TGT),which showed that the levels of 25 (OH) D deficiency were positively correlated with the elderly fall risk.Conclusions Daily intake of dairy products,sunlight,calcium and ordinary vitamin D supplement are insufficient among Chinese elderly people.Decreases of muscle strength and balance function among elderly people are related to 25 (OH) D levels.Exercise,not Vitamin D supplement,can reduce fall risk through improving muscle strength and balance function in elderly people.

Objective To investigate the relationship among serum levels of suppressors of cytokine signaling-3(SOCS-3),insulin-like growth factor binding protein-1(IGFBP-1) and hyperglycemia in critically ill children. Methods The 64 critically ill children who suffered from sepsis,after surgical repair of congenital heart dis-ease by extracorporeal circulation, and after surgery of severe traumatic from January 2009 to January 2012 in Depart-ment of Pediatric Intensive Care Unit( PICU) of Guangzhou Women and Children′s Medical Center were selected as the research object. According to the blood glucose levels on admission, the 64 children were divided into the normal glu-cose group and the hyperglycemia group. The 15 cases of healthy children in the same period in Guangzhou Women and Children′s Medical Center were selected as the healthy control group. The levels of blood glucose,insulin,SOCS-3,IG-FBP-1 and insulin resistance index( HOMA-IR) were measured and compared among groups when they were on ad-mission. Results (1) The blood glucose of the hyperglycemia group was significantly higher than those in the normal glucose group and the healthy control group[(9. 83±2. 48) mmol/L vs (4. 82±0. 76) mmol/L,(4. 49±0. 81) mmol/L] (P<0. 05). (2) Both of the insulin and HOMA-IR in critically ill children with hyperglycemia were significantly higher than those in the other 2 groups[9. 68(5. 85,17. 35) mU/L vs 4. 08(2. 00,7. 41) mU/L,2. 83(2. 00,10. 10) mU/L;4. 30(2. 70,7. 66) vs 0. 87(0. 39,1. 94),0. 52(0. 38,2. 56)](P<0. 05). (3) The serum SOCS-3,IGFBP-1 levels among the hyperglycemia group,the normal glucose group and the healthy control group were not of significant difference (P>0. 05). (4) The blood glucose was positively correlated with the insulin and HOMA-IR(r=0. 455,0. 773,P<0. 05),but there was no significant correlation among the blood glucose,insulin,HOMA-IR,SOCS-3 and IGFBP-1(P>0. 05). Con-clusions In the critically ill children,hyperglycemia was related to the insulin resistance which can not be evaluated through changes in serum levels of SOCS-3 and IGFBP-1. In addition,it cannot be excluded that the critically ill chil-dren may have insulin resistance and pancreaticβ-cell dysfunction simultaneously.

Continuous blood purification is continuous, slow removal of water and solute molecules in the blood.So,it is a treatnent modality of organ supports.With the development of blood purification in children,application of continuous blood purification in children is beyond the field of renal replacement therapy.Treatment indications include systemic inflammatory response syndrome,sepsis,multiple organ dysfunction syndrome,acute respiratory distress syndrome,bums,etc.Continuous blood purification has become the most important treatment in pediatric intensive care unit.Because of the children's physiological features,blood purification equipment and the disease spectrum, continuous blood purification requires higher technology and presents more difficultits.

Hashimoto’s thyroiditis (HT) is strictly related to a cellular immune response with lymphatic infiltration of the thyroid gland by T and B cells, as well as a humoral immune response leading to specific antibody production. The synchronous appearance of HT and papillary thyroid cancer (PTC) indicates an immunological link between the two entities. In this article, we provide a review of the etiology of HT and the potential immunological mechanism of HT coexistent with PTC, which will provide references for new ideas for clinical diagnosis and treatment of HT coexistent with PTC in the future.

After Puerarin Eyedrops was optimized,its stabilities in the conditions of high tempera- ture,low temperature,strong light and constrant temperature with constant humidity were studied by reversed phase HPLC.The analysis method was rapid,specific and precise.The average recovery was 99.42%.The results showed that the Puerarin Eyedrops had good thermostability and photostability.The shelf life of the Puerarin Eyedrops was 2 years.

Objective:To invetigate the use of targeted anticancer drugs in our hospital during 2011-2013 to provide reference for the clinical rational drug use. Methods:The utilization of anti-tumor molecular targeted drugs in our hospital during 2011-2013 was retrospectively analyzed in respect of consumption sum, DDDs and DDC. Results:The proportion of consmption sum of anti-tumor mo-lecular targeted drugs in the total consumption sum was increased year by year, and that in 2012 and 2013 was over 60%. Protease ki-nase inhibitors accounted for 41. 29%-73. 41% in the consumption sum of anti-tumor molecular targeted drugs; monoclonal antibody accounted for 19. 40%-30. 88%; the other drugs accounted for 7. 19%-27. 83%. The consumption sum and the number of receiving medication patients of rituxan and cetuximab were not synchronized well, and the patients were born the higher cost. Conclusion:The use of anti-tumor molecular targeted drugs in our hospital is increased year by year, and the oral administration of anti-tumor molecular targeted drugs is with especially wide application.

The improving brain circulation is the most important aspect in protecting brain from the ischemic trauma. This frauma on pathophysiology includes the two stages which are related to different treatments. The paper induced some therapeutic progress, such as thrombolytic therapy, the use of anticoagulation agents, antiplatelet drugs, neuroprotectine agents, intracerebral transplantation, antiedama, and reducing intracranieal pressure.

Newborn due to low birth weight caused by preterm or fetal growth restriction will have an adverse effect on kidney development. In adulthood,the long-term adverse effects of low birth weight are associ-ated with a variety of kidney disease. Current studies suggest that low birth weight may participate in a variety of kidney disease development and progression by affecting the nephron number,the function of the vascular struc-ture and the endocrine level. This paper mainly reviews the relationship between low birth weight and kidney dis-ease.