Objective To investigate the relationship between CT performance of Hver cirrhosis and liver fibrosis factors.Methods 89 hospitalized patients were graded for the CT performance of the liver cirrhosis:O class (have no cirrhosis),Ⅰ,Ⅱ,Ⅲ,Ⅳ class.Then to compare the examination data of senlm liver fibrosis factors:type Ⅲ procollagen(Prop),Ⅳ collagen(Ⅳ-C),laminin(LN),hyaluronie acid(HA).Results O class(have no cirrhosis in the sufferer):four index signs all has no increase.Ⅰ class:the Ⅳ-C slightly goes up.Ⅱ class:Ⅳ-C,the HA slishtly goes up.Ⅲ class:the three of Ⅳ-C,HA,LN rises,the HA is distinct.Ⅳ class:four index signs all go up obviously.Conclusion With the CT performance of liver cirrhosis is obvious,the hver fibrosis factors get obvious,too.Both of them get positive correlation.

Objective To observe the protective effect of recombinant plasmid pCDNA3.1(+)/Ngb during focal cerebral ischemia in rat brain.Methods Fifty-four male Wistar rats were randomly divided into three groups:normal saline(NS)control group,plasmid control group,and recombinant neuroglobulin group.NS,plasmid pCDNA3.1(+)and recombinant plasmid pCDNA3.1(+)/Ngb were respectively injected into two sites of the rat cerebra1 cortex 24 hours before induction of neocortical focal ischemia by occlusion of the right middle cerebral artery for 24 hours.The condition of local ischemic damage,expression of bcl-2 and the apoptosis in neural cells were confirmed by staining with 2% 2.3.5-triphenyltetrazolium chloride,in-site cell apoptosis detection,indirect immunofluorescent staining and Western blotting,respectively.Results The extent of cerebral infarction tissue and the apoptosis cells in the pCDNA3.1(+)/Ngb group were significantly reduced than those in other control groups(P

Objective To investigate the status of AmpC β-lactamase and extended spectrum β-lactamases (ESBLs) production and drug-resistance characteristic of Enterobacter cloacae.Methods Enterobacter cloacae was isolated and cultured from variety of specimens from April 2005 to July 2007. VITEK32 automatic microbial analytical system was applied to performing identification of bacteria and susceptibility test. ESBLs were confirmed by NCCLS double disc assay, and three-dimensional test was used to detect AmpC.Results A total of 63 strains of Enterobacter cloacae were isolated, including 13(20.6%) single AmpC producing strains, 24(31.8%) single ESBLs producing strains, 6(9.5%) both AmpC and ESBLs producing strains, and 20(38.1%) neither AmpC nor ESBLs producing strains. The resistance of lactamases producing strains was significantly higher than that of lactamases non-producing ones. Especially in both AmpC and ESBLs producing strains, the drug resistance was serous.Conclusion The prevalence of AmpC β-lactamases and/or ESBLs producing strains is a higher level in Fuyang area. The drug resistance of AmpC β-lactamases producing Enterobacter cloacae isn′t identical to that of ESBLs producing one. Different categories of antibacterials should be chosen according to the different types of producing lactamases.

Objective To develop the darkroom(tent) needed for X-ray film developing in the field conditions.Methods Three layers of colored cloth in red,black and army green were used for light-proving.Methods as field tent was used to support ground and create space.Results Tested in comprehensive field medical support drilling,this tent could develop film in 10 minutes despite the high temperature in the tent.The result was accepted by doctors.Conclusion It is waterproof,windproof and lightproof,and can develop satisfactory films in practical drill.

Objective To describe the financial management specification of the medical device account in hospital. Methods The stock and consumption statistics situation of the medical equipment and consumable material from technical angle were elaborated,as well as the equipment maintenance of the administrative regulations. Results The regulations can be used to inquire,count,report conveniently,and acquire the use of supplies and equipment maintenance in details. Conclusion It will enable the work of medical engineering disciplines to have foresightedness,regularity,independency and determinism.

Objective To set up proper management specifications for hospital bidding,since there is no national and military unification patterns and standards as the administrative regulations of medical equipment bidding at present.Methods Through the unceasing exploration and summary,the article proposed a series of scientific,feasible and effective management plan as a standard model,methods and steps in the medical equipment bidding program.Results According to the management plan,the tender of the production,group formation,implementation of bidding,and statistical information could be procured.Conclusion Its implementation will cause the medical devices bidding program to be more perfect.

AIM: To study the pharmacodynamics of Guanhuang Ganmao Capsule. METHODS: Antiviral effect of Guanhuang Ganmao Capsule was investigated by infecting chicken embryo with N_1H_1; its antipyretic action was evaluated with rabbit pyrogentic test induced by bacterial endotoxin; anti-inflammation by rats swelling test with injection of 1% agar and mice abdominal capillary permeability test with dimethylbanzene; the antitussive activities were investigated through testing cough times induced by ammonium hydroxide and sulfur dioxide in mice; the expectorant activities of it were studied by testing the amount of phlegm secreted in mice; The antibacterial effects in vivo were evaluated in mice with induced systematic infection. RESULTS: Guanhuang Ganmao Capsules had obvious antiviral action, antipyretic action, anti-inflammation and antitussive action. It took expectorant effect. The capsules markedly reduced death rate of staphylococcus aureus infection in mice. CONCLUSION: Guanhuang Ganmao Capsules possess antiviral; antipyretic; antitussive and expectorant effects as well as anti-inflammation

CD72 is a B cell specific receptor that exists in multiple alternative splicing forms. Eight novel alternative splicing forms of CD72 were identified from the spleenocytes of BALB/C mice. Two very unique intron sequences were found in those alternative splicing forms. One kind of splicing variants retained the intron1 in the mRNA. This intron can be translated into 32 amino acid residues without changing the reading frame of the whole proteins. Another kind of splicing variants used an alternative 3' splice site in intron 3(3'AS) which led to premature termination of its encoded protein. The differential expression of the CD72 splicing variants were compared in BALB/C and NZB/W mice that were at different stage of systematic lupus erythematosis(SLE) disease development. It was found that 1) splicing forms containing 3'AS was rare in all samples examinated; 2) splicing forms containing two ITIM domains and transmembrane domains were more abundant in BALB/C mice than in NZB/W mice, even in some cases the two ITIM domains were separated by the intron 1; 3) a shorter splicing form with both exon2 and exon3 missing was expressed highly in terminally diseased NZB/W mice.These results suggested an important role of CD72 alternative splicing forms in B cell receptor signaling and in SLE.

Objective To clone rat neuroglobulin (NGB) gene and construct its eukaryotic expression vector. Methods The total RNA was extracted from Wistar rat brain and the full length cDNA encoding NGB was obtained by RT-PCR. After the sequence was confirmed by sequencing and BLAST, it was inserted in the eukaryotic expression vector pCDNA3.1 (+), then the sequence and reading frame were confirmed by two restriction endonucleases and sequencing. Results The NGB gene was cloned with four bases mutated and its eukaryotic expression vector was constructed. Conclusion NGB expressed in Wistar rat brain. NGB gene was successfully cloned and inserted in eukaryotic expression vector.

Serum prealbumin (PAB) and plasm free amino acids were determined in normal and zinc deficient preschool children. In zinc deficient children, the prealbumin level was much lower than normal;cystine and .proline were higher but most of other amino acids and essential amino acids, branched chain amino acids, essential amino acids/total amino acids, branched chain amino acids/nonbranched chain amino acids were signifcantly lower than those of normal children.