AIM: To study the pharmacodynamics of Guanhuang Ganmao Capsule. METHODS: Antiviral effect of Guanhuang Ganmao Capsule was investigated by infecting chicken embryo with N_1H_1; its antipyretic action was evaluated with rabbit pyrogentic test induced by bacterial endotoxin; anti-inflammation by rats swelling test with injection of 1% agar and mice abdominal capillary permeability test with dimethylbanzene; the antitussive activities were investigated through testing cough times induced by ammonium hydroxide and sulfur dioxide in mice; the expectorant activities of it were studied by testing the amount of phlegm secreted in mice; The antibacterial effects in vivo were evaluated in mice with induced systematic infection. RESULTS: Guanhuang Ganmao Capsules had obvious antiviral action, antipyretic action, anti-inflammation and antitussive action. It took expectorant effect. The capsules markedly reduced death rate of staphylococcus aureus infection in mice. CONCLUSION: Guanhuang Ganmao Capsules possess antiviral; antipyretic; antitussive and expectorant effects as well as anti-inflammation

Serum prealbumin (PAB) and plasm free amino acids were determined in normal and zinc deficient preschool children. In zinc deficient children, the prealbumin level was much lower than normal;cystine and .proline were higher but most of other amino acids and essential amino acids, branched chain amino acids, essential amino acids/total amino acids, branched chain amino acids/nonbranched chain amino acids were signifcantly lower than those of normal children.

Objective To investigate the relationship between CT performance of Hver cirrhosis and liver fibrosis factors.Methods 89 hospitalized patients were graded for the CT performance of the liver cirrhosis:O class (have no cirrhosis),Ⅰ,Ⅱ,Ⅲ,Ⅳ class.Then to compare the examination data of senlm liver fibrosis factors:type Ⅲ procollagen(Prop),Ⅳ collagen(Ⅳ-C),laminin(LN),hyaluronie acid(HA).Results O class(have no cirrhosis in the sufferer):four index signs all has no increase.Ⅰ class:the Ⅳ-C slightly goes up.Ⅱ class:Ⅳ-C,the HA slishtly goes up.Ⅲ class:the three of Ⅳ-C,HA,LN rises,the HA is distinct.Ⅳ class:four index signs all go up obviously.Conclusion With the CT performance of liver cirrhosis is obvious,the hver fibrosis factors get obvious,too.Both of them get positive correlation.

Objective To establish the Valproate (VPA) exposure-induced autism spectrum disorder (ASD) model at early pregnancy in rats,and to study the changes of social interaction test and neuroimmune system in autism model rats,and discuss the pathogenic mechanism.Methods Twelve-week-old rats were randomly divided into the non-exposed group (n =10) and the VPA-exposed group (n =10).Their babies were respectively just the normal control group (n =10) and the model group (n =10).The autism-like social behavior was evaluated via the social interaction test.The level of interleukin (IL)-1β,IL-4,IL-6,interferon-gamma (IFN-γ),transforming growth factor-beta (TGF-β) in mothers and offspring were detected by using enzyme-linked immunosorbent assay (ELISA).The histopathological damage in brain was observed with immunohistochemical staining.Results The score of social interaction test in the model group [(-163.16 ± 101.92) scores]was lower than that in the normal control group [(132.73 ± 114.63) scores] (t =-6.100,P ＜ 0.05).The levels of IL-1β,IL-4,IL-6,IFN-γ in VPA-exposed group were higher than those in the non-exposed group (t =5.883,6.394,5.655,5.393,all P ＜ 0.05),while the level of TGF-β was lower than that in the non-exposed group (t =-6.726,P ＜ 0.05).The levels of IL-1β,IL-4,IL-6,IFN-γ in the model group were higher than those in the normal control group(t =3.058,3.048,6.670,5.486,all P ＜ 0.05),while the level of TGF-β was lower than that in the normal control group (t =-6.516,P ＜ 0.05).The change trend of the level of cytokines in the serum of ASD model rats was similar to the change trend of the level of cytokines in the serum of VPA exposed rats.The result of social interaction test the approach-avoidance score was inversely correlated with the levels of IL-1β and IL-4 (r =-0.802,-0.781,all P ＜ 0.05).The result of immunohistochemical staining showed the expressions of glial fibrillary acidic protein (GFAP) and ionized calcium-binding adapter molecule 1 (IBA1) in the model group were higher than those in the normal control group.Conclusions The main neuroimmunological pathogenesis mechanism can be explained as follows:at early pregnancy,the maternal cytokines skewing influenced by VPA exposure can make immune activation,induce immune system dysfunction and affect the brain growth and development,which result in autism-like behavior in offspring.

Objective To explore the diagnostic value of multi-slice spiral CT MIP-MPR image in regional lymph node metastasis of colonic carcinoma.Methods CT findings of 33 cases with pathological confirmed colonic carcinoma were analyzed retrospectively . All patients were underwent three-phase 256-slice CT dynamic enhancement scanning.All data were processed in workstation,to obtain routine MPR and MIP-MPR image.The regional lymph nodes of colon cancer were counted,and divided into 3 groups accord-ing to the short diameter of lymph node:group I (3.0-5.0 mm),group Ⅱ(5.1-10 mm),and group Ⅲ(10.1 mm up).The diag-nostic sensitivity of CT MPR and MIP-MPR image in checking out lymph nodes of different groups was compared by paired test.Re-sults The average value(x ±s )of lymph node number of three groups with routine MPR and MIP-MPR image were:group I, 3.66±1.99,6.09±2.44 (t=1 1.6,P <0.05);group Ⅱ,3.72±1.79,4.03±1.89 (t=3.73,P <0.05);group Ⅲ,1.21±1.31, 1.27±1.46(t=1.44,P >0.05).There were statistical differences in group I and group Ⅱ.Sensitivity of MIP-MPR image in chec-king out regional small and middle lymph nodes of colonic carcinoma was higher than routine MPR image.Conclusion Multi-slice spiral CT MIP-MPR image is a good way to checking out regional small and middle lymph nodes of colonic carcinoma.

Objective To describe the financial management specification of the medical device account in hospital. Methods The stock and consumption statistics situation of the medical equipment and consumable material from technical angle were elaborated,as well as the equipment maintenance of the administrative regulations. Results The regulations can be used to inquire,count,report conveniently,and acquire the use of supplies and equipment maintenance in details. Conclusion It will enable the work of medical engineering disciplines to have foresightedness,regularity,independency and determinism.

Objective To set up proper management specifications for hospital bidding,since there is no national and military unification patterns and standards as the administrative regulations of medical equipment bidding at present.Methods Through the unceasing exploration and summary,the article proposed a series of scientific,feasible and effective management plan as a standard model,methods and steps in the medical equipment bidding program.Results According to the management plan,the tender of the production,group formation,implementation of bidding,and statistical information could be procured.Conclusion Its implementation will cause the medical devices bidding program to be more perfect.

CD72 is a B cell specific receptor that exists in multiple alternative splicing forms. Eight novel alternative splicing forms of CD72 were identified from the spleenocytes of BALB/C mice. Two very unique intron sequences were found in those alternative splicing forms. One kind of splicing variants retained the intron1 in the mRNA. This intron can be translated into 32 amino acid residues without changing the reading frame of the whole proteins. Another kind of splicing variants used an alternative 3' splice site in intron 3(3'AS) which led to premature termination of its encoded protein. The differential expression of the CD72 splicing variants were compared in BALB/C and NZB/W mice that were at different stage of systematic lupus erythematosis(SLE) disease development. It was found that 1) splicing forms containing 3'AS was rare in all samples examinated; 2) splicing forms containing two ITIM domains and transmembrane domains were more abundant in BALB/C mice than in NZB/W mice, even in some cases the two ITIM domains were separated by the intron 1; 3) a shorter splicing form with both exon2 and exon3 missing was expressed highly in terminally diseased NZB/W mice.These results suggested an important role of CD72 alternative splicing forms in B cell receptor signaling and in SLE.

Objective To develop the darkroom(tent) needed for X-ray film developing in the field conditions.Methods Three layers of colored cloth in red,black and army green were used for light-proving.Methods as field tent was used to support ground and create space.Results Tested in comprehensive field medical support drilling,this tent could develop film in 10 minutes despite the high temperature in the tent.The result was accepted by doctors.Conclusion It is waterproof,windproof and lightproof,and can develop satisfactory films in practical drill.

Currently,heart failure is one common disease causing serious damage to children's health and life which has high missed diagnosis rate and mortality.Accurate and effective evaluation of pediatric cardiac function is of great importance in the treatment,surveillance and prognosis of disease.Tei index was first presen-ted by Japanese scholars as a new comprehensive indicator of global (combined systolic and diastolic)myocar-dial function.It was defined as a ratio between the sum of isovolumic times (ICT and IRT)and ejection time (ET).This index can be measured by a simple,reproducible and non-invasive method regardless of changes in the heart rate or ventricular shape and other external factors.The sensitivity for ventricular dysfunction could be high.Tei index has been used more and more often in the clinical evaluation of cardiac function especially in children.This paper reviews the measurement methods,related factors and pediatric clinical application progress of Tei index.