Objective To explore the effect of antidepressant adjuvant treatment for chronic prostatitis (CP),and to find an effective treatment for the clinical therapy of CP.Methods 88 patients with CP were randomly divided into two groups,the control group 41 patients were treated with the antibacterials moxifloxacin,the treatment group 47 patients were treated with the moxiiloxacin treatment and paroxetine adjuvant therapy.The clinical symptoms,life quality (QOL) and Zung depression scale score of the two groups were evaluated and compared.Results After four weeks of treatment,voiding difficulty endless,urinary urgency,perineal discomfort symptoms of the treatment group were improved significantly compared with the control group (x2 =23.563,18.348,15.874,all P ＜ 0.05).After four weeks of treatment,QOL,Zung depression scale scores the treatment group were more significant decline than before treatment and the control group after treatment (t =0.554,0.615,0.382,0.336,all P ＜ 0.05).Conclusion Antidepressant adjuvant therapy improves the psychiatric symptoms of CP patients,which can effectively improve the clinical efficacy of antibiotic therapy of CP.

Objective To discuss the pathogenesis and clinical characteristics of acute relapsing pancreatitis .Methods The clini-cal data and relevant data of 274 acute pancreatitis cases from January 2010 to and December 2012 were analyzed ,of which one group of 231 cases were first onset and the other group of 43 cases were relapsing .Results The probability of relapse of acute pan-creatitis were highest from 1 month to half a year after first onset (20 cases ,accounting for 46 .51% );biliary factors were the major causes of first onset and relapse cases ,where the relapsing group was higher than first onset group ,with statistical significance (P<0 .05);the relapsing group had more severe symptoms of hyperlipidemia than the first onset group ,with significant difference (P<0 .05) ,The relative risk (OR value) of relevant factors were higher than biliary factors ;improper diet ,alcohol and other factors of the relapsing group were lower than the first onset group ,without statistical significance (P> 0 .05);there was no significant difference between the occurrence rate of severe pancreatitis and the occurrence rate of surgeries and deaths .Conclusion Biliary diseases and hyperlipidemia are closely related with the relapse of acute pancreatitis .

Objective To investigate the clinical efficacy and safety of endoscopic variceal ligation (EVL) combined with endoscopic variceal sclerosis (EVS) for treating patients with esophageal variceal bleeding (EVB).Methods Ninety patients with EVB were divided into observation group and control group by treatment methods with 45 patients each.The observation group was given EVL combined with EVS and the control group was given EVL.The elimination of varicosity after the first treatment,time and times required to eliminate,rehaemorrhagia,varicosity recurrence,complication and prognosis of the 2 groups were observed.Results The elimination rate of varicosity of observation group was 97.8％(44/45),the control group was 93.3％(42/45),there was no significant difference (P＞ 0.05).The elimination rote of varicosity after the first treatment of observation group was significantly higher than that of control group,the time and times required to eliminate were significantly lower than those of control group [71.1％(32/45) vs.48.9％(22/45),(17.24±5.65) d vs.(36.01 ± 11.81) d,(1.42 ±0.47) times vs.(1.87 ±0.61) times,P ＜ 0.05 or ＜0.01].The rates of early-onset rehaemorrhagia,delayed rehaemorrhagia and varicosity recurrence of observation group were significantly lower than those of control group [4.4％(2/45) vs.17.8％(8/45),6.7％(3/45) vs.22.2％ (10/45),2.2％ (1/45) vs.22.2％ (10/45),P ＜0.05].The rate of complication of observation group was significantly lower than that of control group [11.1％ (5/45) vs.42.2％ (19/45),P ＜0.01].Conclusion The EVL combined with EVS is an effective method for emergency hemostasis and preventing rehaemorrhagia in the patients with EVB.

Objective To select health individual and test serum total bilirubin and direct bilimbin in order to provide a reference range for the establishment of appropriate reference interval in this region.Methods From October to December in 2009,314 of physical examination population of Beijing Hospital were selected by questionnaire and laboratory test excluding liver and gallbladder diseases or metabolic diseases.Roche and Prodia reagents were used to test TBIL and DBIL,respectively.Reference interval was calculated by sex group and compared with the existing reference interval.Results The level of TBIL had a significant difference between different genders(P ＜ 0.01).The level of TBIL had a significant difference between two kinds of reagents(P ＜ 0.01).Using Roche reagent,the reference interval of TBIL was 7.1 ～ 27.2 μmol/L for man,and 4.8 ～ 20.9 μmol/L for woman,and the reference interval of DBIL was 1.4 ～6.8 μmol/L for man,and 0.9 ～5.7 μmol/L for woman.Using Prodia reagent,the reference interval of TBIL was 9.5 ～ 35.7 μmol/L for man,and 6.8 ～ 28.9 μmol/L for woman,and the reference interval of DBIL was 1.3 ～ 7.0 μmol/L for man,and 1.0 ～ 6.6 μmol/L for woman.Conclusions The level of TBIL and DBIL in physical examination population was higher than the existing reference interval.It is necessary to modify the existing reference interval and establish reasonable reference interval for different regions and gender,respectively.

Objective To evaluate the treatment of obstructive jaundice caused by bile duct strictures after hepatic transcatheter arterial chemoembolization in hepatic tumor patients. Methods A retrospective review (Jun 1994 - Mar 2010) of databases at two institutions (Zhejiang Provincial People's Hospital and Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine) identified 15patients with obstructive jaundice caused by liver bile duct stricture after transcatheter arterial chemoembolization. There were 7 cases of primary liver cancer, 5 patients of liver hemangioma, 3 cases of metastatic liver cancer including 2 cases of colonic cancer and one of pancreatic cancer. Obstructive jaundice appeared in a period of 5 months to 16 months after TACE. The median time was 9 months. Results The obstructive jaundice was relieved by surgically constructed hepatobiliary drainage or PTC+stenting treatment in 13 cases and PTCD in 2 cases. All patients of hepatic hemangioma were doing well after treatment. Two cases of primary liver cancer patients with obstructive jaundice after TACE were followed up for 2 years with no recurrence of hepatic carcinoma and bile duct obstruction. The other 8 patients were followed up from 3months to 18 months until to their death from primary disease progress. Conclusions Surgery and or PTCD plus stent can effectively relieve the obstructive jaundice caused by TACE in benign or malignant liver tumors.

The health check-up results of 11 514 subjects in 2011 at Beijing Hospital were retrospectively analyzed,including the results of 13C urea breath test for detection of Helicobacter pylori (Hp) and HbA1c test.The results showed that the prevalence rate of Hp infection was significantly higher in females (31.77％) than that of males (29.36％) (x2 =7.355,P ＜0.05) ; the highest prevalence was found in 35-60 y age group (31.17％).Hp positive rates in subjects with body mass index (BMI) ≥28kg/m2 and central obesity were 31.55％ and 30.44％,respectively; there were no significant differences from those with normal weight and non central obesity.Subjects with positive Hp infection had higher levels of HbA1 c,compared to those with negative Hp infection (t =-2.276,P =0.01).In subjects with BMI ≥24 kg/m2 and central obesity,HbA1c level was higher in Hp positive subjects than that in Hp negative ones (P ＜0.05).Diabetes mellitus was positively associated with Hp infection (OR 1.282; 95％ CI 0.941-1.799),particularly in obese patients (OR 1.459 ; 95 ％ CI 1.070-2.071).The results indicate that Hp may be involved in the impairment of glucose regulation,particularly in those with higher body mass index.

Objective To explore the reversion of astragaloside and NMDA receptor antagonist against the hyperphosphorylation of protein Tau induced by fetal intrauterine distress in neonatal rats.Methods The analysis of variance of factorial design was setted up two intervention factors which were fetal intrauterine distress (two levels:no disposition;a course of fetal intrauterine distress)and the drugs (three levels:iv Saline;iv astragaloside;iv MK -801).When the neonatal rats grew to 12weeks,the hippocampus was removed from the neonatal rats.Detected the content of glutamate in the hippocampus of rats by high performance liquid chromatography.The expression of protein Tau which includes p -AT8Ser202 and GSK -3β1H8 in the hippocampus of rats were detected by the methods of immunohistochemistry staining.Results Fetal intrauterine distress could significantly up -regulate the content of glutamate,which was not affected by MK -801,in the hippocampus of neonatal rats which was reduces by the astragaloside (P <0.05).And both influences presented subtracting effects (P <0.05).Fetal intrauterine distress and the drugs do not affect the total protein Tau in the hippocampus of rats (P <0.05).Fetal intrauterine distress could up -regulate the hyperphosphorylation of protein Tau in the hippocampus of neonatal rats which could be reduced by astragaloside and MK -801 (P <0.05).And the influences between fetal intrauterine distress and the drugs presented subtracting effects (P <0.05 ).Conclusion Our results indicate that fetal intrauterine distress reduce the hyperphosphorylation of protein Tau in neonatal rats though up -regulating the content of glutamate.GSK-3βis the key protein in this signaling pathway.

BACKGROUND:Daclizumab can be special y combined with the inerleukin-2 receptor on the surface of activated T cells in human body, and this method can reflect the close of interleukin-2 receptor thus inferring the effect of induction therapy. At present, the daclizumab has been widely used in renal transplantation, but there is no consensus on its clinical application in liver transplantation. OBJECTIVE:To investigate the expression of serum CD25+T cells and soluble interleukin-2 receptor in the patients receiving daclizumab for liver transplantation during perioperative period. METHODS:A total of 58 patients received orthotopic liver transplant for the first time were included and then the patients were randomly divided into two groups:control group (n=28) and treatment group (n=30). The patients in the two groups were treated with tacrolimus, mycophenolate mofetil and corticosteroids triple immunosuppressive regimen. The patients in the treatment group received immune induction therapy with daclizumab, and the patients in the control group did not receive daclizumab. RESULTS AND CONCLUSION:Flow cytometry and enzyme-linked immunosorbent assay showed the expression levels of CD25+T cells in the treatment group were significantly lower than those in the control group at different time points after liver transplantation (P<0.01);and the expression levels of soluble interleukin-2 receptor in the treatment group were lower than those in the control group during transplantation and at the first day after transplantation (P<0.05, P<0.01). At 6 months after transplantation, the incidence of acute rejection was decreased in the treatment group (P<0.01). The results indicate that daclizumab can effectively suppress the expression level of CD25+T cells, as wel as the expression level of soluble interleukin-2 receptor in the peripheral blood in the early stage of liver transplantation, thus effectively reducing the rate of acute rejection.

Objective To investigate the effect of nursing intervention on bowel cleaning of patients with senile constipation during electronic bowel preparation for colonoscopy.Methods Forty patients with senile constipation undergoing routine bowel preparation for colonoscopy were treated with nursing intervention.The bowel cleaning effect and the satisfaction degree after bowel cleaning were assessed. Results The qualification rate of bowel cleaning reached 92.5%,and the excellence rate of cleaning comfort 95.0%.Conclusion The pertinent nursing intervention to the elderly patients with constipation undergoing electronic colonoscopy may improve the rate of cleaning the bowels as well as their level of comfort so that the patients can live smoothly through the electronic colonoscopy.

Objective To investigate the relationship between serum leptin levels and insulin resistance(IR)in obesity and hypertensive patients and their role on the occurrence of hypertension or/and obesity.Methods Serum glucose,insulin,total cholesterol(TC),triglycerides(TG),HDL－ C,LDL－ C,uricacid(Ur),insulin sensitivity index(ISI),urinary albumin excretion(UAE)and serum leptin concentrations were determined in 66 men with essential hypertension and 67 male normotensives.The correlations between leptin and IR,and they and body mass index (BMI),blood pressure and other parameters were analyzed.Results Serum leptin levels were signifcantly higher in hypertensive and obesity subjects than in normotensive and nonobesity ones.ISI of nonobesity was higher than obesity in normotensive group,but both obesity and nonobesity ISI was higher in normotensive group than in hypertensive one .No significant change in obesity and nonobesity was found for the decreased levels and ISI(r=－ 0.51,P<0.01 and r="－" 0.38,P< 0.05,respectively ) and the most singificant factors that affected serum leptin levels were BMI,waist hip ratio and ISI and the factors that affected ISI in order of importance were BMI,SBP,DBP,TG,Ur and leptin in obesity subjects of both hypertensive and ISI in above mentioned patients.The multiple regression analysis indicated that BMI was closely correlated with leptin,ISI and TG in obesity patients of both hypertensive and normotensive groups. Conclusion The increase in serum leptin levels in obesity is highly correlated with ISI and is correlated with lipid metabolism.leptin resisitance may has a indirect action in occrrence of hypertension and the correlation with IR remain to be sutdied.