Objective To investigate the genetic patterns of iris pigmentat mutation in the white hair black eye ( WHBE) rabbits.Methods To construct six two-generation families between WHBE rabbit and Japanese white rabbit, and the quantity of individuals had different eye colours in F1 and F2 generations were recorded and analyzed.Results χ2 test showed there was no significant difference between observed values and expected values in the mode of autosomal domi-nate inheritance ( P>0.05) , while there were significant differences between observed values and expected values in the autosomal recessive inheritance and sex-linked genetic pattern(P<0.05).Conclusions Iris pigmentat mutation in WHBE rabbit has a monogenic character due to autosomal dominant mutation.

Aldosterone ; blood ; Humans ; Hyperaldosteronism ; classification ; diagnosis ; Hypertension ; complications ; Mass Screening ; Renin ; blood ; Renin-Angiotensin System

Female ; Humans ; Maternal Health Services ; Maternal Mortality ; Pregnancy

China ; Humans ; Occupational Diseases ; prevention & control

ObjectiveTo develop a method of location of prenatal female mouse reproductive tract based on paraffin block serial sections of female fetuses, and get high quality paraffin sections of fetuses containing female reproductive tract (FRT).And to investigate the role of Wnt5a during the development of FRT of prenatal mice. Methods The sex of fetuses at gravidity 15.5 days( G15.5d), G17.5d, G19.5d) and G21.5d was identified by polymerase chain reaction(PCR),and the female fetuses were embedded in paraffin block, the specimen was sectioned serially . One of every four sections was stained by hematoxylin-eosin(HE), and the next one being used later.The images of specimen were taken with digital camera and the serial sections were obtained. The paramesonephric duct /uterus were located and recognized. Immunohistochemistry was used to determine the location and intensity of Wnt5a staining in the middle of the paramesonephric duct / uterus. Results The morphology of the paramesonephric duct /uterus was recognized first and the sections of fetuses with paramesonephric ducts were obtained. The intensity of Wnt5a immunohistochemical staining was increased from G15.5d to G21.5d in mesenchymal cells(P<0.01).Conclusion Wnt5a plays a marked role in early period of female mouse reproductive tract, and is possible to be a key factor to induce uterus differentiation and development.

Mesenchymal stem cell (MSC) resides in a relatively hypoxia niche (volume fraction 1％～7％)compared to normoxia (20％).MSC cultured in hypoxia condition shows different biological features from those in normoxia condition.Hypoxia inducible factor-1 α (HIF-1 α),as one of main transcription factors in hypoxia,participates in the regulation of cell proliferation,differentiation,apoptosis,glycolysis and angiogenesis.Recent researches show that HIF-lα also plays an important role in regulating the survival,differentiation and migration of MSC.It turns out that hypoxia can improve survival and migration of MSC,enhance chondrogenesis but inhibit adipogenesis through HIF-lα and its downstream genes.However,whether hypoxia promotes or impedes osteogenesis remains controversial.Such differences can be caused by variances of tissue origination,oxygen concentration and culture condition.The comparison of different researches should be based on the same experiment condition.This review focuses on the influence of hypoxia on proliferation,apoptosis,differentiation and migration of MSC.

China ; Genetic Predisposition to Disease ; Humans ; Hypertension ; genetics ; Retrospective Studies

G protein-coupled receptors(GPCRs)mediate cell signaling transduction of most hormones and neurological transmitters and behave as the key targets for drug research and development.Recently,the evidence of allosteric modulation of GPCRs has been revealed.Allosteric modulators have the ability to selectively tune responses only in tissues in which the endogenous agonist exerts its physiological effects,and have the potential for greater receptor subtype selectivity.The GPCRs allosteric modulators have been found and some of them have been in clinical use.Under the strategy of allosterism on structure activity relationship and target directed screening,more and more GPCRs allosteric modulators will be developed in the future.

Environmental arsenic content is influenced by natural condition, microorganism and human activity.Certain natural conditions favor arsenic mobilization to the environment.Some microorganisms adapt in high arsenic environment, which is an important factor for arsenic biotransformation and mobilization in the environment.Moreover, human activities can intensify arsenic accumulation in the environment.The environmental arsenic accumulates rapidly through the food chain and enters human bodies to be detoxicated through methylation.Water arsenic pollution is one of very serious public health problems, which not only causes acute and chronic toxicosis, but also increases human susceptivity to many kinds of diseases.

The energy metabolism of septic patients is serious disorder.The major manifestation is protein degradation,the enhancement of glyconeogenesis and the adipose lipid mobilization.This may be link to abnormal ways of inflammatory media,the hormone secretion and ubiquitin-proteasome caused by sepsis.The discussion on high metabolic mechanism of sepsis,has great significance for guiding clinical practice,promoting targeted interventions and treatment measures and improving the prognosis of patients.This article summarized the progress on hyper metabolism in sepsis.