Objective To explore the expression of Bcl-2 and calretinin(CR)in enteric ganglion cells in different developmental conditions and its significance.Methods The immunohistochemical staining was carried out to detect the expression of Bcl-2 and CR in enteric ganglion cells at different intestinal segments from tissue samples of 40 hirschsprung disease(HD) cases(age,26 d to 12 years old),10 Hirschsprung's allied disease(HAD) cases and 10 normal controls.Results In immature ganglion cells,there was a strong expression of Bcl-2,and no positive to or poor expression of CR.In mature ganglion cells,there was a strong expression of CR but no positive Bcl-2 staining.Conclusion The specific expression of Bcl-2 and CR may play an essential role in judging if the ganglion cells are mature.Bcl-2 might be of importance in the maintenance and restore of the fuction of enteric immature ganglion cells.

A method for the determination of Pt、Pd、Ru、Rh 、Ir and Au in geological samples was studied. The sample was fused by sodium pe roxide and determined by a double focusing high resolution inductively coupled p lasma mass spectrometer “ELEMENT” after the separation and enrichment by co-precipitation with Te. Detection limit of the method was between 1～9ng/g and the recovery was ＞90%. Our analytical results for some domestic or interna tional reference materials were in agreement with the certified values.

Objective To investigate the influence of rosuvastatin on inflammatory factors and immune func-tion in chronic obstructive pulmonary disease(COPD)with pulmonary heart disease.Methods 80 COPD patients with pulmonary heart disease were randomly divided into two groups,40 cases in each group.The control group was treated with conventional medicine comprehensive treatment,the observation group was given rosuvastatin based on the control group.The inflammatory cytokines (TNF -α,IL -1 and hs -CRP)changes,immunoglobulin IgM,IgG and IgA changes situation,and heart,lung function changes related indicators were compared between the two groups before and after the treatment.Results After treatment,the TNF -α,IL -1 and hs CRP levels of the observation group were significantly lower than those before treatment(t =145.5,35.35,57.5,all P <0.05)and those of the control group(t =160.75,11.45,32.99,all P <0.05).After treatment,the IgM,IgG and IgA levels of the observa-tion group were significantly lower than those before treatment (t =4.89 of 18.29,3.42,all P <0.05)and those of the control group (t =2.55 9.67,2.342,all P <0.05 ).After treatment,the FEV1 ,FEV1 /FVC and LVEF of the observation group were significantly higher than those before treatment(t =40.44 33.64,33.79,all P <0.05),while the PAP level was significantly lower than that before treatment (t =24.12,P <0.05),and the FEV1 ,FEV1 /FVC and LVEF of the observation group were significantly higher than those of the control group after treatment(t =16.30, 15.82,18.72,all P <0.05),while the PAP level was significantly lower than that of the control group after treatment (t =13.43,P <0.05).Conclusion Combined rosuvastatin therapy for COPD patients with pulmonary heart disease can effectively reduce body inflammatory response,improve immune function and myocardial contractility,so it can promote functional recovery of heart and lung in patients.

To test whether Con A has antirejection effect on mouse islet allograft, 600-700 handpicked islets of C57BL/6 mice was transplanted beneath the kidney capsule of the streptozotocin induced diabetic BALB/C recipients. In control group the mean survival time (MST) of the grafts was 14.1?2.4 days. Treatment with Con A significantly prolong the MST of the islet allografts. In group with Con A treatment of the donor islets prior to the transplantation the MST was 25?5.4 days. The MST increased sigificantly after ip injection of Con A to recipient mice. The combined treatment of islet and recipient with Con A resulted in a prolongation of MST to 25.2?4.4 days. The experiments concerning the mechanisms of the antirejection effect of Con A showed that Con A was able to combine with the islet cell membrane well masking perhaps the histocmpatibility antigen. Furthermore the lymphocytes of mice after i. p Con A injection suppressed the lymphocyte proliferation reaction of the svnergic mice to mitogen.

Acupressure ; Acupuncture Points ; Child ; Child, Preschool ; Enuresis ; physiopathology ; therapy ; Female ; Humans ; Male ; Treatment Outcome ; Urination

Objective-To-discuss-the-value-of-fast-track-surgery-(-FTS-)-in-perioperative-period-of-laparoscopic-cholecystectomy-.-Methods-We-selected-200-cases-of-laparoscopic-cholecystectomy-in-our-hospital-from-January-2012-to-December-2012.The-cases-were-randomly-divided-into-either-traditional-group-(control-group)-or-fast-track-surgery-group-(FTS-group),-with-100-cases-in-each-group-.The-operation-time-,-intraoperative-blood-loss-,-time-to-get-out-of-bed-after-operation-,-time-to-intake-semi-liquid-diet,-time-to-flatus,-time-to-defecation,-and-length-of-hospital-stay-were-compared-.-Results-Compared-with-the-control-group-,-the-FTS-group-had-shorter-time-to-semi-liquid-diet-[(11.3-±2.0)-h-vs.(50.2-±8.7)-h,-t=-43.976,-P=0.000],-shorter-ambulation-time-[(6.2-±1.5)-h-vs.(14.3-±1.7)-h,-t=-35.728,-P=0.000],-shorter-flatus-time-[(12.0-±4.4)-h-vs.(24.9-±5.4)-h,-t=-18.519,-P=0.000],-shorter-defecation-time-[(15.8-±5.3)-h-vs.(25.2-±4.3)-h,-t=-13.773,-P=0.000],-and-shorter-postoperative-hospital-stay-[(3.2-±1.3)-d-vs.(4.2-±2.1)-d,-t-=-4.048,-P=0.000].-Conclusion-Fast-track-surgery-is-feasible-and-safe-,being-helpful-for-patient-’-s-postoperative-recovery-.

Laboratory animal industry forms an independent, comprehensive system responsible for the production, use and management of laboratory animals.Great advances have been made in this field in China over the more than thirty years of development.However, due to the great development and changes during this long time, there are also some issues which need to be amended and changed.To compare the concepts of experimental animals in China and developed countries such as USA.The key concepts on the laboratory animals are not well or clearly defined in the regulatory documents in China, which hinder the healthy development and effective regulation in this field, as well as the care and use of laboratory animals in research and education.In this article, we discuss the necessity of timely amendment and standardization of the key concepts and definitions related to laboratory animals, and the importance and impact of the key concepts on the development of animal industry in China.

[ ABSTRACT] AIM:To study the effect of rapamycin ( Rap) on the proliferation, invasion, adhesion, apoptosis and autophagy of human adenocarcinoma A549 and resistant A549/DDP cells treated with cis-diamminedichloroplatinum ( DDP) .METHODS: Human adenocarcinoma A549 and resistant A549/DDP cell lines were cultured.The inhibitory effects of Rap alone or combined with DDP on A549 and resistant A549/DDP cells were detected by MTT assay.The in vitro invasion abilities of the 2 cell lines treated with Rap alone or combined with DDP were detected by Transwell methods. The in vitro adhesion abilities of the 2 cell lines treated with Rap alone or combined with DDP were detected by adhesion experiments.The apoptosis of A549 and resistant A549/DDP cells induced by Rap alone or combined with DDP was ana-lyzed by flow cytometry.The cell autophagy marker proteins beclin-1 and LC3 in A549 and resistant A549/DDP cells trea-ted with Rap alone or combined with DDP were detected by Western blotting.RESULTS:Compared with Rap or DDP a-lone group, the combination of Rap and DDP significantly inhibited the proliferation, invasion and adhesion of A549 and resistant A549/DDP cells in vitro, and promoted the cell apoptosis and autophagy marker proteins beclin-1 and LC3 expres-sion ( all P<0.05) .CONCLUSION:Rap enhances the effect of DDP through promoting the cell autophagy, thereby in-hibiting the proliferation, invasion and adhesion of A549 and resistant A549/DDP cells and inducing the cell apoptosis with a synergistic effect.

This article introduced five cases of professor JIANG Jian using later generation's prescriptions of Qingwei Shegan Tang,Qingkong Gao,Xietong Shen Fang,Jianbei Jingutong Fang,Juantong San,Juhe Wan,and Shen xiao Gualou San to treat pain syndrome of gastralgia,hypochondriac pain,headache,backache,and breast-ache. During the treatment of pain syndrome,professor JIANG Jian’ clinical experience by using later generation's prescriptions was extraordinary,deserved to learn an reference.

A simple thin layer chromatography ultraviolet densitometric method has been established for the separation and quantitative determination of Puerarin in plasma. Using ethyl acetate : metha-nol: water : toluene : absolute alcohol ( 100 : 15.5: 13.5: 2 :13.5 V/V ) as the developing system, 5?l supernatant of the sample extracts were spotted on high performance sillica gel G glass plates and developed. The separeted spots were scanned using Shimadzu CS-910 TLC scanner, the results were calculated by the areas of spots with Rf 0.47. The mininum detectable quantity of Puerarin was 7.5ng/spot. The plasma concentration-time curve of Puerarin in rabbits after single iv 30mg/kg was best fitted to a two- compartments open model. The T1/2? , T1/2? (3 were 5.7, 27.5 min respectively. This showed that Puerarin is distributed and eliminated in a fairly rapid rate in rabbits.