OBJECTIVE To investigate the clinical characteristics and the present condition of infective diabetic foot. METHODS A total of 100 cases with infective diabetic foot were classified into the wet gangrene and composite gangrene, and treated by the systemic and local modality in combination. RESULTS The cure rate of the wet gangrene and composite gangrene was 29.5% and 18.2%, respectively and the effective rate was 83.3% and 68.2%, respectively. CONCLUSIONS The combined therapy is effective for the treatment of diabetic foot.

Laboratory animal industry forms an independent, comprehensive system responsible for the production, use and management of laboratory animals.Great advances have been made in this field in China over the more than thirty years of development.However, due to the great development and changes during this long time, there are also some issues which need to be amended and changed.To compare the concepts of experimental animals in China and developed countries such as USA.The key concepts on the laboratory animals are not well or clearly defined in the regulatory documents in China, which hinder the healthy development and effective regulation in this field, as well as the care and use of laboratory animals in research and education.In this article, we discuss the necessity of timely amendment and standardization of the key concepts and definitions related to laboratory animals, and the importance and impact of the key concepts on the development of animal industry in China.

Objective To compare the effect of different sevoflurane induction methods on hemodynamics and immune function in elderly patients with tumor. Methods One hundred and sixty elderly patients with tumor underwent general anesthesia were enrolled, the patients were divided into observation group and control group according to the anesthesia method with 80 cases each. The observation group was received induction method of initial concentration of 2%sevoflurane and increased by 1% per 3 times breathing and then to a final concentration of 4%- 5%. The control group was received 5% sevoflurane induction concentration. The anesthesia induction time, intubation time, recovery time and untoward reaction were recorded. The changes of heart rate, mean arterial pressure (MAP), oxygen saturation of pulse (SpO2), bispectral index (BIS) and CD3+, CD4+, CD8+, CD4+/CD8+were observed. Results There were no statistical differences in BIS, heart rate, SpO2, CD3 +, CD8 + and incidence of untoward reaction between 2 groups (P>0.05). The MAP at immediate intubation in observation group was significantly higher than that in control group:(69.38 ± 10.42) mmHg (1 mmHg=0.133 kPa) vs. (58.52 ± 6.12) mmHg, but the MAP at after intubation was significantly lower than that in control group: (77.12 ± 11.31) mmHg vs. (90.13 ± 9.24) mmHg, there were statistical differences (P<0.05). The anesthesia induction time and intubation time in observation group were significantly longer than those in control group:(110.1 ± 14.2) s vs. (101.4 ± 15.8) s and (341.2 ± 57.3) s vs. (310.4 ± 60.8) s, but the recovery time was significantly shorter than that in control group: (271.2 ± 31.3) s vs. (321.8 ± 41.5) s, there were statistical differences (P<0.05). The CD4+and CD4+/CD8+at end of surgery and 1 d after surgery in observation group were significantly higher than those in control group, CD4+:0.241 ± 0.027 vs. 0.182 ± 0.034 and 0.332 ± 0.039 vs. 0.284 ± 0.042, CD4+/CD8+: 0.84 ± 0.12 vs. 0.69 ± 0.13 and 1.09 ± 0.52 vs. 0.93 ± 0.43, there were statistical differences (P<0.05). Conclusions Induction method of gradually increasing concentration of sevoflurane enables the hemodynamics of elderly tumor patients more stable. In addition, this method can reduce the inhibitory effect on T lymphocytes to some extent.

Improving the quality of newborns is a health development strategy, which has attracted global attention. Adverse pregnancy outcomes, including preterm birth, low birth weight and small for gestational age, are major causes of perinatal mortality and disability. Based on review of international and national publications pertaining to associations between stressful life events during pregnancy and adverse pregnancy outcomes from 2007 to 2023, this review summarizes the correlation between stressful life events during pregnancy and adverse pregnancy outcomes, including preterm birth, low birth weight and small for gestational age, and describes the underlying biological mechanisms. Previous studies have demonstrated the associations between maternal stressful life events during pregnancy and adverse pregnancy outcomes, and the underlying mechanisms mainly include neuroendocrine regulation, inflammation and microbiota pathways; however, the exact mechanisms remain unclear until now. Further studies to identify the critical window period for the association between stressful life events and adverse pregnancy outcomes, and unravel the pathogenesis of adverse pregnancy outcomes are warranted, so as to provide insights into reduction of adverse pregnancy outcomes.

OBJECTIVE: To observe the protective effect of soybean isoflavones (SI) on the heart function of the rats with adriamycin induced heart failure. METHODS: Thirty adult male SD rats were divided into 5 groups:normal control (NC) group, adriamycin (ADR) group, L-SI group, M-SI group and H-SI group. SI of 30, 60, 120 mg.kg(-1).d(-1) was orally administered through a stomach tube once a day for 6 days in L-SI group, M-SI group and H-SI group, respectively. The other two groups were given the same amount of normal saline the same way. Then ADR of 10 mg/kg was given intraperitoneally once to copy the model of heart-failure. The MedLab-U/4c biological signal collecting system was used to record and analyze the LVSP of the rats. The pathological changes of the cardiomyocytes were observed. RESULTS: As compared with NC group, the LVSP,+/-dp/dt max, Vpm of the ADR group were significantly lower (P<0.05 or P<0.01), but those of the H-SI group were markedly higher than those of the ADR group (P<0.01). Electron microscopic morphometry of the heart samples of the rats in ADR group revealed typical alterations, consisting an increase of collagen content, vacuolation, diminishing of the cardiomyocyte diameter, alteration of myofilaments and Z-lines of myofibers, and myofibrillar degeneration. SI of 120 mg.kg(-1).d(-1) treatment could prevent the loss of myofibrillae and the reduction of myocyte diameter, and the degeneration of myofilaments and Z-lines were reversed by SI. CONCLUSION: SI of 120 mg.kg(-1).d(-1) treatment can relieve the toxic effect of ADR on myocardium, and also obviously improve the cardiac contractility of heart-failure rats.

Glucose metabolism of cancer cells presents Warburg effect.In resent years,more and more experiments demonstrate that the therapeutics based on aerobic glycolysis pathway in cancer cells restrict energy and inhibit tumor proliferation through the inhibition of a variety of moleculars,genes and signal pathways.These can provide an opportunity for targeted cancer therapies and possess enormous potential advantages and broad prospects in clinical application.

Regulatory T cells (Treg),a group of negative regulatory cells,have four subsets:CD4+Treg,CD8 + Treg,NKT Treg and DN Treg cells.They play an essential role in the inhibitive immune-regulation and might be the key factors of neoplasms immune escape.These mechanisms include inhibiting the effector cell function by inhibitory cytokines,killing effector cells by granzyme and perforin,competition and inhibiting IL-2,and affecting Treg differentiation and proliferation by regulating the function of CTLA-4,etc.Tumor immunotherapies targeting Treg and related immunosuppressive factors,such as remove Treg or controling the numbers and functions,enhances the immune response against tumors,which might offer a new method of tumor immunotherapy.

Objective To analyze the surgical treatment and pathological manifestations in patients with hyperparathyroidism. Methods The clinical data of 368 patients undergoing parathyroidectomy with hyperparathyroidism in Peking Union Medical College Hospital between Oct 1974 and Jan 2009 were retrospectively analyzed. Results 368 cases underwent parathyroidectomy. There were 249 women (67.7%) and 119 men (32. 3% ). Parathyroid lesions were located in normal positions in 318 cases (86. 4% ), and in ectopic positions in 50 cases (13.6%). The pathology of 264 cases (71.7%) was adenoma, 91cases (24.7%) was hyperplasia, 11 cases (3.0%) was carcinoma, and two cases was parathyroid cyst. Postoperatively 257 cases (68.0%) presented with hypocalcaemia, and 159 cases (66. 0% ) recovered within 7 days. Conclusions The main pathological diagnosis was adenoma. It is common for a ectopic parathyroid gland in which a parathyroid lesion is located.

AIM:To investigate the dissolvability of thundergodvine extract(Tripterygium wilfordii) and triptolide in different dissolvent in transdermal drug delivery system and to study the relationship among dissolvability of extract,and its active component and mean diameters of suspension. METHODS:To determine dissolvability of extract,dissolvability of active component and mean diameters by precipitation method,dissolvability of active component method and mean diameters method. RESULTS:① solvents in sequence of dissolvability of extract,alcohol,isopropanol,ethyl,IPM,water,glycerol;② solvents in sequence of dissolvability of active component alcohol,isopropanol,ethyl,IPM,water,glycerol;③ mean diameters of suspension in sequence:IPM,ethyl,alcohol,isopropanol. CONCLUSION:① The change in dissolvability between extract and active component is identical;② The relationship between the changes in mean diameters and the dissolvent of active component is negative correlation on the whole.

Objective To Study the effect of long-term triptolide use on the content of the normal rat plasma ACTH.Methods 20 male SD rats were divided into 2 groups randomly,and gavaged for 7 weeks.The control group was 10 with 2%propylene glycol 2ml/kg,bid;the group of Triptolide was 10 with 2% the mixture of propylene glycol and Triptolide 20?g/kg,bid.We used radioimmunoassay to detecet the content of ACTH in rat's plasma and immunocytochemistry to detecet the expression of ACTH in pituitary. Results The content of ACTH in the group of the Triptolide had significant difference as compared with control group.The expression of ACTH in the group of the Triptolide was higher than that of control group. Conclusion These results suggested that one of the possible mechanism of the Triptolide in the HPAA might be exciting pituitary ACTH neurons,thus causing the content of ACTH increasing.