China ; Congresses as Topic ; Humans ; Integrative Medicine ; methods ; trends ; Medicine, Chinese Traditional ; methods ; trends ; Societies, Medical ; organization & administration

The prevalence of obesity,type 2 diabetes,and other chronic non-communicable diseases is increasing rapidly world-wide.Most recent epidemiological data showed that China is now in an era of obesity,and under a huge burden of the complications related with obesity,diabetes,as well as cerebral-and cardiovascular disorders.Strenuous efforts,comprehensive strategies,effective measures,and practical solutions must all be taken to combat against these chronic diseases.

OBJECTIVE To investigate the major pathogenic bacteria and drug resistance of hospital infection in pediatrics unit,and to provide laboratory evidence for clinical diagnosis and treatment.METHODS Pathogenic bacteria were isolated from patients who suffered from hospital infection in pediatrics unit from 2004 to 2006.the method was taken to carry out the sensitive test and bacteria identification by VITEK32,Streptococcus pneumoniae,Moraxella catarrhalis and Haemophylus influenzae were assayed with French Bio-Merieux API System ATB STREP5 and ATB HAEMO,respectively.RESULTS Totally isolated strains were 489.Of them,108 strains of coagulase negative Staphylococcus(CONS),being 22.1%.58 strains were S.haemolyticus.The isolated rate of CONS was 53.7%.The drug test for meticillin-resistant coagulase negative Staphylococcus(MRCNS) showed multiple drug-resistance.The detection rate of the ESBLs from the Escherichia coli and the Klebsiella pneumoniae was 18.9% and 12.5%,respectively.CONCLUSIONS MRCNS are the major pathogens in pediatrics unit.The detectable rate of MRCNS is high.Glycopeptide antibiotics are the first-choice drugs for MRCNS infection.

Objective To evaluate the feasibility and clinical efficacy of laparoscopic biliary reoperation. Methods From May 2000 to May 2007, 105 patients received laparoscopic biliary reoperation, including choledocholithotomy and T-tube drainage in 96 cases, Roux-en-Y anastomosis of the gallstone/hepatic-duct in 5, and liver resection in 4. Results The operations were completed in all the 105 patients without severe complications. The operation time was 60-185 min [mean, (125.3?23.2) min]. Intraoperative blood loss was 15-310 ml [mean, (21.1?8.6) ml]. The patients stayed at hospital for 5-9 days after the operation. The 5 patients, who underwent Roux-en-Y anastomosis of the gallstone/hepatic-duct, were followed up for 6 to 65 months [mean, (13.2?8.6)months]. Partial liver resection was performed in 2 cases (resection of the left hepatic lobe in 2, and the right lobe in 2); they were followed up for 8-25 months [mean, (10.2?3.6)months]. In the 96 patients, who received laparoscopic choledocholithotomy and T-tube drainage, the follow-up were lasted for 3-72 months [mean, (13.2?9.6)months]. None of the patients who were followed up had recurrence of gallbladder stone. Conclusions Laparoscopic biliary reoperation is feasible and safe with minimal trauma to patients.

OBJECTIVE To evaluate the pathogens in department of orthopedics infection and the bacterial resistance to antibiotics,and to provide reference of clinical antibiotic therapy.METHODS A total of 603 pathogenic strains isolated from patients with bone and joint purulent infection,from Apr 2004 to Apr 2007 were retrospectively analyzed with the results of their bacterial cultures and antibiotics sensitivity tests by VITEK32,a part of antibiotics sensitivity tests by K-B and Etest.RESULTS The inspection rate of Pseudomonas aeruginosa,Staphylococcus aureus,S.haemolyticus,S.epidermidis,and Acinetobacter baumannii were 17.08%,14.76%,12.94%,12.27% and 9.65%,respectively.Meticillin-resistant S.aureus(MRSA)was higher than 60.00%,pathogen rate of Gram-positive cocci,Gram-negative bacteria,and Candida were 47.76%,43.78%,and 7.96%,respectively.Staphylococcus were major pathogens of bone and joint purulent infection,multidrug-resistant Staphylococcus infections have increased.CONCLUSIONS Bone and joint purulent infection is mainly caused by Staphylococcus which resist to many antibiotics,A.baumannii and P.aeruginosa are also often found these years.Routine blood culture before using of antibiotics should be taken.The sensitive antibiotics are selected and adjusted according to the results of blood culture and sensitivity test.

Objective To investigate the effect of atorvastain on plasma levels of nitric oxide(NO) and alpha-granule membrance protein-140(GMP-140) in patients with acute cerebral infarction.Methods 94 cases with acute cerebral infarction were randomly divided into the atorvastain group(48 cases) and the control group(46 cases).Plasma NO and GMP-140 were determined by the method of nitrate reductase and enzyme-linked immunosorbent assay in atorvastain group and control group at moment of hospitalization and after four weeks.Results Plasma NO and GMP-140 of atorvastain group after treatment were statistically lower than that of controls(P0.05).Conclusion Atorvastain can reduce plasma levels of NO and GMP-140 in patients with acute cerebral infarction.It can ameliorate brain injury after ischemia and has protective effect on the ischemia cerebral tissue.

Objective To investigate the effect of the selective cyclooxygenase-2 inhibitor celecoxib on the expression of PGE2 and VEGF induced by IL-1? in A549 cell lines.Methods A549 cells were incubated with IL-1? in the presence or absence of celecoxib.After indicated incubation time,cells were harvested to detect VEGFmRNA by reverse transcription-polymerase chain rection(RT-PCR),the medium was collected to determine the concentration of PGE2 and VEGF by enzyme-linked immunosorbent assay(ELISA).Results After Celecoxib intervention,the formation of PGE2 and VEGF expression decreased more than control group(IL-1? induced),the difference being significance(P

Objective To investigate the distribution,the drug-sensitivity tests and drug-resistance of bacteria in patients with lower respiratory tract infections diseases in respiratory intensive care units(RICU).Methods Retrospective study of the clinical data,the distribution and the drug-sensitivity of bacteria of 178 cases with mechanical ventilation in RICU.The drug-sensitivity were examined by Kribry-Bauer(KB)paper method and minimal-inhibitory-concentration(MIC)by agar double dilute method.Results A total of 326 strains bacteria were obtained in 178 patients.Among 326 strains G-bacteria were the most common kinds of bacteria(78.2%);main kinds of the G-bacteria were pseudomonas areuginosa(30.4%)and baumanii(23.0%);fungus was the second commonest kind of bacteria(16.6%).Most of the G+ bacteria were staphycoccus aureus.In 107 cases more than two kinds of bacteria were obtained.The drug sensitivity test in vitro showed that these strains were multiresistant to commonly used antibiotics,and drugs whose sensitive rate was over 70% included meropenem,impienem,cefoperazone/sulbactam,amikacin and piperacillin/tazabactam,the sensibility of vancomycin to G+ bacteria being 100%.Conclusion Pseudomonas areuginosa,baumanii and fungus are the main pathogens of the patients in RICU;meropenem,impienem,cefoperazone/sulbactam,amikacin and vancomycin are effctive antibiotics for treating lower respiratory tract infection in RICU.There is mixed infection by various bacteria,and some strains are multiresistant.We should monitor drug-sensitivity to bacteria dynamic and use antibiotics reasonably.

To investigate the antibiotic resistance of bacteria isolated from bile, the bile from the surgical in-patients with bile duct infection from June 1995 to December 1998 was cultured and the distribution and antibiotic resistance of the isolates (not including anaeobes) were analyzed. The results showed that the cultured positive rate in the specimens was 69. 76 % (143/205). Among the isolates, most of them were E. coli and P. aeruginosa (31. 74 % and 18. 56 %, respectively), while Enterobacter, K. pneumonia and Enterococci were also commonly seen. All the isolates were resistant more or less to the commonly used antibiotics.

Objective To explore the mechanism of the microglia inflammatory response through observing the changes of interleukin-1 receptor associated kinase-4(IRAK-4) expression in murine N9 microglia cells under hypoxia.Methods N9 cells were exposed to 3%O2,5%CO2,92%N2 for 1,3,6,12 and 24 h respectively.The IRAK-4 protein level was detected by Western blotting and the celluar change was observed by laser scanning confocal microscope(LSCM).The TNF-? level was measured by ELISA.Results After hypoxia,the expression of IRAK-4 protein was increased in a time-dependent manner.The elevation began at 1 h after hypoxia,increased significantly at 3 h(P0.05).The fluorescence intensity of IRAK-4 also increased with the extension of anoxic time.The TNF-? level change were similar.There were significant positive correlations between the expression of IRAK-4 and TNF-?(P