Background and purpose:Reduced peripheral blood platelet count is a common toxicity in patients with hematological malignancy after chemotherapy.The purpose of this study was to observe the eff icacy and safety of recombinant human thrombopoietin(rhTPO)in the treatment of thrombocytopenia induced by chemotherapy.Methods:A total of 25 patients with solid tumor,who developed thrombocytopenia induced by chemotherapy(PLT≤70?109/L) after the f irst cycle of chemotherapy(control group),was studied by self-cross control.6-24 h after the second cycle of chemotherapy(treatment group) with identical scheme of the f irst cycle chemotherapy,they were given subcutaneous injection of rhTPO 15 000 U/d for 7 to 14 consecutive days or until platelet count ≥100?109/L or the increasing count ≥50?109/L.Results:The mean platelet count of the patients after rhTPO treatment was higher at different time points of the treatment group than that of the control group.The minimal PLT count of the treatment group and the control group after chemotherapy were(80.3?39.30)?109/L and(34.7?21.2)?109/L(P0.05).The time of PLT count to recover was found to be more than 75?109/L and 100?109/L in treatment group after chemotherapy was(9.8?4.2)d and(12.8?3.6)d,compared to(19.1?4.5)d and(24.3?1.4)d(P

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Objective: To explore the role and molecular mechanism of high intensity focused ultrasound (HIFU) inhibits pancreatic cancer cell proliferation and invasion via regulating miR-1297/PTEN axis. Methods: With treating the cells with HIFU for 1 to 3 seconds, the effect of HIFU on cell proliferation, invasion and apoptosis of PANC-1 cells in vitro was examined by CCK-8, Transwell and Annexin V-FITC/PI double staining flow cytometry, respectively. The effect of HIFU on expression of miR-1297 and PTEN were measured by qPCR and Western blotting. Moreover, the relationship between miR-1297 and PTEN was examined by dual luciferase report assay. Western blotting was used to detect the effect of HIFU on the expression of Akt, p-Akt (308) and p-Akt (473) after transfected with miR-1297 inhibitor and PTEN-siRNA. Results: HIFU treatment significantly inhibited the cell proliferation, invasion and promoted apoptosis of PANC-1 cells (all P<0.01), which was associated with inhibition of miR-1297 expression and activation of PTEN expression in pancreatic cancer cells (all P<0.01). Moreover, miR-1297 directly binds to the 3′ UTR of PTEN mRNA to suppress its expression in PANC-1 cells. Further, HIFU exposure could significantly inhibit the expression of the phosphorylation of Akt (P<0.01). Conclusion: HIFU inhibits PTEN and blocksAkt signaling by down-regulating miR-1297, thereby suppresses the occurrence and progress of pancreatic cancer.