Objective To study the deep vein thrombosis (DVT ) in lower extremities of patients with or without cardiovascular disease after fracture .Methods Five hundred and eighty-four frac-ture patients were divided into cardiovascular disease (CVD) group (n=235) and non-cardiovas-cular disease (NCVD) group (n=349) .Patients in CVD group were further divided into subgroup A (with essential hypertension) ,subgroup B (with CHD) ,and subgroup C(accompanying essen-tial hypertension and CHD) .Incidence of DVT in CVD group ,NCVD group ,subgroups A-C at different ages after fracture was compared .Results The incidence of DVT was significantly high-er in CVD group than in NCVD group (P<0 .01) ,in two groups on days 0-7 after fracture than on days 8-14 or >14 after fracture ,and in patients with their age ≥60 years than in those with their age <60 years .Conclusion DVT is easier to occur in CVD patients with their age ≥ 60 years than in those with their age <60 years after fracture .DVT usually occurs within 1 week af-ter fracture .Importance attached to high risk population and measures taken as soon as possible against it can reduce DVT after fracture .

Nitrogen mustard encapsulated with liposomes was administered to the rabbits with acute experimental serum sickness(AESS). Then the serum levels of anti-BSA antibodies and circulating; immune complex(CIC), CH50, and WBC count were determined. The ACPase activity of spleen macrophages was measured. And the sections of renal tissues, after HE-stained, were studied for the accumulation of CIC and the pathological changes. It was found that liposome-encapsulated nitrogen mustard can increase the macrophage activity and in turn aggravate the renal damages of AESS rabbits.The results suggest that the renal damages of AESS rabbits can immunopatholo-gically be attributed to the increased activity of macrophages.

The afferent connections to the periaqueductal gray in the rat were examined by means of retrograde axonal transport method using horseradish peroxidase (HRP).The microinjection syringe needle or micro-electrophoretic pipette was inserted stereotaxically by an oblique approach through the cerebellum. HRP was introduced into the dorsal, lateral, ventrolateral areas of periaqueductal gray or periventricular gray of ventriculus Ⅲ at posterior commissure level respectively.The results were as follows:1. Injections of HRP in the dorsal area of periaqueductal gray(PAG).Numerous labeled cells were consistently found in the paraventricular nucleus, dorsomedial and ventromedial hypothalamic nuclei, posterior hypothalamic nucleus, medial mamillary nucleus, zona incerta, lateral and ventral nuclei of periaqueductal gray, superior colliculus, inferior colliculus, dorsal raphe nucleus and periventricular gray of ventriculus Ⅳ. In some cases, a smaller number of cells was found in the zona reticulata of the substantia nigra, and lateral hypothalamic nucleus. In one case, the enzyme was injected into the dorsal area of PAG, which diffused to the superior and inferior colliculi, resulted in labeling of Forel's field and reticular nucleus of thalamus.2. Injection of HRP in the lateral area of PAG.HRP-positive cells were observed within the bed nucleus of the stria terminalis, paraventricular nucleus, anterior hypothalamic nucleus, ventro-medial hypothalamic nucleus, medial thalamic nucleus, nucleus interstitialis magnocellularis, commissural posteriors, zona incerta, ventral nucleus of PAG, zona reticulata of substantia nigra, formatio reticularis of mesencephalon, dorsal raphe nucleus, and periventricular gray of ventriculus Ⅲ and Ⅳ. In some cases, a small number of cells was found in the supraoptic nucleus, superior and inferior colliculi.3. Injection of HRP in the ventrolateral area of PAG.In all cases the greatest number of HRP-positive neurons was shown in the supraoptic nucleus, paraventricular nucleus, periventricular gray of ventriculus Ⅲ, lateral nucleus of PAG, zona reticulata of substantia nigra, dorsal raphe nucleus and locus coeruleus. The labeled cells were mainly located ipsilaterally. But the labeling of supraoptic nucleus was observed bilaterally. In one case the enzyme diffused to the reticular formation, a smaller number of cells was found in nucleus cuneifomis and mesencephalic reticular formation of more caudal position.4. Injection of HRP in the periventricular gray of ventriculus Ⅲ.Following the injections of HRP in the periventricular gray of ventriculus Ⅲ, numerous HRP-positive cells were consistently observed in the bed nucleus of the stria terminalis, supraoptic nucleus, paraventricular nucleus, ventromedial nucleus of hypothalamus, medial nucleus pars medialis of thalamus, zona incerta, ventral nucleus of PAG, zona reticulata of substantia nigra, dorsal raphe nucleus and locus coeruleus.

This paper is the first to report skin infection of baby panda caused by Trichophyton gy-pseum, Chorioptes panda and Demodex ailuropodae. The clinical manifestation and laboratory examination of this disease were also reported. We took a comprehensive measure for the prevention and treatment of this disease. Marked efficacy was obtained

Objective To evaluate the efficacy of PGE_1 for the treatment of hyperbilirubinemia due to viral hepatitis.Methods A prospective,randomized,single blind and parallel control clinical study enrolled 100 patients with icterohepatitis,who were randomized 1∶1 to two treatment groups;Each group was intravenously infused daily over a six-week treatment period:(1)200?g of PGE_1 injection;(2)20ml of potassium-magnesium aspartate injection.Results The PGE_1 treated patients were more superior in the recoveries of physical characteristics,icteric index and liver functions to the control group.The statistical difference of the PGE_1 group from the potassium-magnesium aspartate in the treatment of icterohepatitis occurred in the 3rd week of the therapy(P0.05).Conclusion PGE_1 is effective in the treatment of the acute or chronic viral hepatitis and the hyperbilirubinemia caused by early stage severe hepatitis.

Objective To study clinical features and its diagnosis and treatment of alcoholic cirrhosis.Methods 68 cases with alcoholic cirrhosis were retrospectively analyzed,with cirrhosis from hepatitis B as control.Results Alcoholic cirrhosis accounted for 8.5% of in-patient cirrhosis in the correspomding period,mean age being(53.5?14.6),and with more than 135g alcohol intake per day which persisted over 10 years.Such signs significantly increased as dark complexion,liver palms,spider naevi,hepatomegaly,ynecomastia,hypertrophy of parotid gland,?-glutamyltansferase(GGT),ratio of aspartate aminotransferase(AST) to alanine aminotransferase(ALT),mean corpuscular volume(MCV),uric acid(UA),serum ferritin(SF) rose apparently,usually combined with hepatogenic diabetes,peptic ulcer,hyperuricemia,hyperlipemia.Peculiar complication were alcohol withdrawal syndrome,gort,gouty arthritis,aseptic necrosis of femur.Among them,53 cases(77.9%) improved clinically,9 cases(13.2%) showed no effect,6 cases(8.8%) died.Conclusions Occurrence of dark complexion,liver palms,spider naevi,hepatomegaly,ynecomastia,hypertrophy of parotid gland and test of GGT,AST/ALT,MCV,UA,SF are helpful to clinic and differentiation diagnosis of alcoholic cirrhosis,abstinence are not able to prevent its development.Long-term therapy aiming at endotoxin toxemia might improve prognosis of alcoholic cirrhosis.

Objective To construct prokaryotic cell expression vector of human Eotaxin mutants.Methods By point mutation,eight amino acid residues in the N-terminal(residues of 3-7)and N-loop(residue 14)regions of Eotaxin were individually mutated to methionine and residue 14 was delleted or methiomine was inserted after the residue 14,and then cloned respectively into prokaryotic cell expression vector-PET30a+.Results Eight N-terminal and N-loop mutants of human Eotaxin and their prokaryotic cell expression vector-PET30a+ were gained.Conclusion The successful construction of prokaryotic cell expression vector of human Eotaxin mutants lays a foundation for their expression and biological activity and for filtering antagonists of CCR3.

Objective To investigate the clinical effect of combined therapy of left and right amlodipine in the treatment of hypertension complicated with perimenopausal syndrome. Methods 50 patients with hypertension associated with peri menopausal syndrome in our hospital from January 2015 to January 2016 were selected,and according to the different treatment and nursing intervention divided into observation group and control group,25 cases in each groups. The control group with routine nursing intervention and benazepril treatment, observation group with amlodipine and comprehensive psychological nursing intervention treatment before and after treatment in two; blood pressure, luteinizing hormone (LH), serum follicle stimulating hormone (FSH), estrogen (E2) level and the changes of anxiety and depression (SAS) (SDS) were recorded in detail, and the relevant data for comparative analysis. Results Amlodipine+comprehensive psychological nursing intervention (observation group) of peri menopausal comprehensive clinical effect is better than that of benazepril+routine nursing intervention patients with hypertension treatment (control group) clinical efficacy, clinical symptoms improved better than the control group, the difference was statistically significant (P<0.05). Conclusion Hypertension patients with perimenopausal syndrome patients choose amlodipine + comprehensive psychological nursing intervention treatment effect significantly, can effectively improve the clinical symptoms and psychological state, and is worthy of clinical application.

Objective To probe the transplantation of skeletal muscle satellite cells of new-born rats for repairing skeletal muscle contusions. Methods Thirty-six Sprague-Dawley adult rats were separated at random into experimental and control groups (including a normal saline control group and a normal control group). Contusions were created on the left hind legs of rats in the experimental group. The cells were cultured and antibodies were identified through immunocytochemical staining and fluorescence labeling. In the experimental group, 0.2 ml of a muscle satellite cell suspension was slowly injected into the medial and lateral heads of the right calf gastrocnemius. The normal saline control group was injected with an equal volume of normal saline solution at the same sites. On the 5th, 10th and 15th day after the operation, EMG wave-amplitude and wave-width were recorded. The recovery rate of the muscle's wet weight and the gray value of a muscle fiber transverse section were determined.Two-factors analysis of variance was performed on the data. Results Before transplantation the muscle satellite cells were bright red after staining and their nuclei were blue after fluorescence labeling. After the operation the number of cells fluorescing and the strength of the fluorescence gradually decreased. The transplanted cells formed myotubes, melted into the muscle tissue and gradually took part in the restoration. The stained muscle satellite cell nuclei were free in intercellular space and melted into the basement membrane gradually. The outlines of the muscle tissue became progressively clearer. EMGs showed the fibrillation potentials as well as positive sharp waves reduced, but they too tended to become similar to those from the normal side. Restoration of EMG wave-amplitude and wave-width in the experimental group was better than in the saline control group and close to those in the normalcontrol group. The gray value of the muscle fiber transverse section area under HE staining was significantly lower at the 5th day post transplantation in both the experimental group and the saline control group than in the normal control group. At the 10th and 15th days post transplantation, the average gray value in the experimental group had increased significantly more than in the saline control group, and it was close to the normal control group average. In the experimental group the recovery rate of muscle wet weight was close to 1, but in the saline control group it was less than 1. Conclusions Transplanting skeletal muscle satellite cells of new-born rats can produce evident effects in renovating injured muscles.

In order to understand the prevalence of the obesity in children and adolescence and provide the basic information for developing scientific strategy for obesity prevention,this paper described the common methods to evaluate the obesity of children and adolescence,and its prevalence,distributions of population and tendency of changing.The paper also analyzed the effects of sex,age,region and socioeconomic status on obesity.