Diagnostic Techniques and Procedures ; standards ; Humans ; Immunohistochemistry ; Lymphoma ; classification ; diagnosis ; pathology ; Pathology ; education ; standards ; Practice Guidelines as Topic ; Reference Standards

Antigens, CD ; metabolism ; Diagnosis, Differential ; Epstein-Barr Virus Infections ; Herpesviridae Infections ; Humans ; Immunohistochemistry ; Lymphoma ; classification ; immunology ; pathology ; virology

Objective To explore the related factors of senile acute pulmonary thromboembolism(PTE) patients,and to observe the prognosis and analyze the strategy of control and prevention to reduce the incidence. Methods 50 elderly patients with acute PTE were selected,related factors were analyzed by using the retrospective investigation,and the prevention and control were administrated.Results The mainly disease occurred of the elderly patients with acute PTE were combined with chronic diseases 42% (21 /50),with lower extremity DVT 26.00%(13 /50)and with recent trauma,fracture and operation 20%(10 /50).Elderly patients with acute PTE who were ≥70 years of age and lying in bed the time≥1 month,were 58%(29 /50)and 62%(31 /50),which were higher than those of the other age <70 years old and lying in bed time <1 month of patients,the difference was statistically signifi-cant(χ2 =9.26,9.80,all P <0.05).Conclusion The related risk factors for elderly patients with acute PTE mainly correlate with age,lying in bed time,chronic diseases,patients with lower extremity DVT and recent trauma,fracture, operation,the variety of factors and overlap each other.Mastering the variety factors of PTE in the nursing process,and taking effective measures to prevent and control the factorscan reduce or avoid the occurrence of acute PTE,and im-prove the prognosis of the patients.

OBJECTIVE To study the phenomenon of hepatitis B surface antigen(HBsAg) and antibody(HBsAb) both positive in blood samples.METHODS Using electro-chemo-luminescence immune system E170 to detect HBsAg and HBsAb in clinical samples and retest the both positive cases by another two reagents for HBsAg and another three reagents for HBsAb.RESULTS All 7332 samples showed 140 both HBsAg and HBsAb positive(1.9%) and 73 of them were retested for HBsAb by another three reagents which gave altogether 24 positive results,which was not related to each other.Those gave higher HBsAb value by E170 showed higher positive rate by Abbott reagent.CONCLUSIONS HBsAg and HBsAb both positive results are not rare.It may be due to the interactions between the patient and the infected hepatitis B virus.Besides,the quality of reagents and correct lab operating procedures may also affect the test results.

Objective To investigate the association between systemic lupus erythematosus(SLE) and polymorphism of cytotoxic T-lymphocyte antigen 4 gene(CTLA-4) and the effect on ANA and anti-dsDNA antibody.Methods The A/G substitution was investigated in the exon 1 position 49 in 92 SLE patients and 60 controls by PCR restriction fragment length polymorphism(PCR-RFLP).ANA and anti-dsDNA antibody were examined by indirect fluorescence immunoassay.Results SLE patients had significantly higher frequencies of the CTLA-4+49GG allele than controls(P

Objective To understand the types and risk factors of human papillomavirus (HPV ) infection among the patients with cervical intraepithelial neoplasia in Chongqing to provide a scientific basis for prevention and treatment of cervical cancer . Methods The patients with TCT screening in many hospital of Chongqing from June 2012 to December 2013 were selected and performed the HPV typing ,9 328 cases succeeded in HPV typing .The main types of HPV infection in different grades of cervical intraepithelial neoplasia were statistically analyzed for determining the risk factors .Results HPV16 was the most common infection type among patients with cervical intraepithelial neoplasia in Chongqing area ,followed by HPV52 ,58 ,33 ,18 .Some factors such as multiple sex partners ,young female with sexual debut and induced abortion were the risk factors of HPV infection .Conclusion Single and high-risk type is the main feature of HPV infection among the patients with cervical intraepithelial neoplasia in Chongqing .The infection rate is higher with the higher grade of cervical intraepithelial neoplasia .Publicity and education for risk factors of HPV infection should be strengthened ,which will help to prevent the occurrence of cervical cancer .

Objective To evaluate the clinical effect of levofloxacin combined with Jingangteng particle in the treatment of patients with chronic pelvic inflammatory disease.Methods According to the random number table, sixty -four patients with chronic pelvic inflammatory disease were divided into control group(n =32)which were treated with levofloxacin and treatment group(n =32)which were treated with levofloxacin and Jingangteng particle for three weeks.The clinical effect,symptoms and signs were evaluated and the adverse reactions between the two groups were observed as well as serum C reactive protein(CRP)were measured before and after treatment.Results The score of symptoms and signs of the treatment group were significantly lower than the control group,the difference was statistically significant [(2.61 ±2.09 )points vs.(5.32 ±3.28)points,t =3.941,P =0.000;(1.47 ± 1.02)points vs.(2.56 ±2.04)points,t =2.703,P =0.008].The level of serum CRP of the control group was (5.46 ± 1.93)mg/L,which was statistically higher than (3.76 ±1.06)mg/L of the treatment group after treatment,the differ-ence was statistically significant (t =4.367,P =0.000).The total effective rate of the treatment group was 96.9%, which of the control group was 78.1%,the difference between the two groups were statistically significant(χ2 =5.142,P =0.023).There was no significant adverse reactions during the treatment.Conclusion Levofloxacin com-bined with Jingangteng particle can obviously improve the symptoms and signs of chronic pelvic inflammatory disease, they have good safety and are worthy of clinical popularization and application.

Cbf?1 gene encodes an osteoblast specific transcription factor that regulates osteoblast differentiation and bone formation. The Cbf?1 gene contains 9 exons, however, the alternative splicing of its exons leads to genesis of multiple Cbf?1 isoforms with differences in transactivation potentials. Several signal transduction pathways, such as Ras MAPK pathway, cAMP pathway, or Smads DPC4 pathway, are involved in regulating Cbf?1 activity or its expression. These studies open new doors in pharmacological control of bone formation and gene or cell therapy of slowing the progression of osteoporosis.

There are still two outstanding problems in comprehensive quality assessment index system of clinical medical undergraduates:contents of assessment is incomprehensive and every index has no quantitative research based weight.This article elaborated on a complete index framework,covering required knowledge,abilities,attitude,morality of clinical medical undergraduates and proposed a set of methods and processes,including literature research method,system analysis method,brainstorming,expert interviews,Delphi method,and some statistical methods,providing a theoretical basis for the development of comprehensive quality assessment index system for clinical medical undergraduates.

Following the development of the socialist market economy and the reformation of the medical safeguard system and the improvement of the higher education reformation,the shortcoming of the medical safeguard system of college students which has started in the period of planned economy has become visible.Through the narration of present situation and the analysis of the reasons to the domestic college students'medical safeguards,this article suggests that our government should establish university student medical service safeguard system which is a perfect and multi-level system and brings the university students'medical safeguard system into the national society medical service safeguard.At the same time,we must strengthen university students'realization of medicine guarantees so as to guarantee them to complete their studies successfully