Pro. Zhang Farong treats diabetes by differentiating signs and pairing drugs, such as mulberry bark and wolfberry bark, epimedium and male cocoon, lycium and mulberry, motherwort and Herba Lycopi. And these usually lead to good curative effect. By Chinese drug pair analysis, it shows the organic combination of correct analysis on disease, defining therapy and agile pairing drugs is of great meaning to such diseases as diabetes.

Objective To study the bacteriostatic effect of the volatile oil from Artemisia argyi. Methods Minimal inhibitory concentration (MIC) was measured with double-fold didution method. Protective effect of the volatile oil from Artemisia argyi were determined by experimental treatment of infected mice in vivo. Results The MIC90 of 3 kind of pathogenic bacteria were 0.78～25 ?L/mL and protective rate for infected mice were 40%～50%. Conclusion The volatile oil from Artemisia argyi has better inhibiting effect on Staply lococcus aureus, Escherichiacoli and Pseudomona than Erythromycin. It has better protective effect for infected mice.

Drug Resistance, Viral ; Endopeptidases ; Hepatitis B virus ; drug effects ; Hepatitis B, Chronic ; virology ; Humans ; Interferons ; pharmacology

Ceramides ; Fatty Liver, Alcoholic ; Humans

Objective To investigate the effect of Hyaluronic acid(HA) on the expression of matrix metalloproteinases(MMPs)-3,9,13 and tissue inhibitor of MMPs(TIMP)-1 mRNA stimulated by interleukin (IL)-1β in chondrocytes from arthritis model in vitro.Methods Different levels of Sodium Hyaluronate(10,20,40μg/ml)were added to culture medium of rat chondrocytes.The ehondrocytes were isolated from rat osteoarthritis model.One hour later,10 ng/ml IL-1β were added to each sample and co-cultured for 24 hours.The expressions of MMP-3,9,13 and TIMP-1 mRNA were detected bv RT-PCR.The concentration of nitric oxide (NO) in the supernatants were measured by Griess reaction.Results HA could down-regulate MMP-3,9,13 mRNA expression but had no effect on TIMP-1 mRNA expression.Moreover,it also had a dose-dependent down regulate effect on nitric oxide synthesis.Conclusion This study demonstrates that HA can down-regulate the TIMP-1/MMPs expression by inhibiting NO production.

In view of the transformation from traditional biomedicine to biology-psychology-social medicine,the comprehensive medical iournals,in order to advance the development of the medicine,should pay attention to the articles on psychology,society and environment with diseases;strictly investigate the ethics in the articles;and publish the articles on the medical humanity promptly.

Objective To observe the expression of glycoprotein non-metastatic melanoma protein B (Gpnmb) in the kidney and urine after ischemic-reperfusion injury (IRI),and explore the relationship between Gpnmb and macrophage phenotypes in the IRI kidney.Methods Male C57BL/6J mice were randomly divided into control group (n =4),sham group (n =4) and IRI group (n =12).Both renal pedieles of mice in IRI group were identified and occluded with microvascular clamps for 30 min.Renal pathological injury was observed by PAS staining.The expression of Gpnmb was examined by real-time PCR and immunofluoresence staining.The location of Gpnmb was observed by flow cytometry and double immunofluoresence staining with F4/80.The mRNA expressions of Gpnmb,CD40,CRR7,CD163 and MMR were examined by real-time PCR.The expression of Gpnmb in the urine was examined by Western blotting and ELISA.Results PAS-stained IRI kidney section showed desquamative epithelia,necrosis debris and a large number of inflammatory cell infiltration.Real-time PCR results showed that there was little expression of Gpnmb in the kidney of control group and sham group.However,the Gpnmb mRNA level in IRI kidneys was highly up-regulated at day 1 and day 2 (both P ＜ 0.01) and followed by a decrease that was similar to the control level at day 3.Double immunofluoresence staining of kidney sections from IRI mice revealed that Gpnmb was predominantly detected in F4/80 positive macrophages.The mRNA expression of Gpnmb was not correlated with M1 macrophage phenotypes CD40 and CCR7,but positively correlated with M2 macrophages phenotypes CD163 and MMR.Western blotting and ELISA result showed that there was significant increase of Gpnmb expression in the urine from IRI mice compared to those of the control group and the sham group (P ＜ 0.01).Conclusions Gpnmb expression is up-regulated in IRI kidney and is associated to M2 macrophages.It may play a role in the process of acute kidney injury.Gpnmb expression is also increased in urine after IR injury and it may be a new biomarker to diagnose AKI.

Objective:To evaluate the influence of cervical collar height on the compressive strength of CAD/CAMzirconia-ceramic restoration.Methods:Zirconia cores were manufactured and divided into 5 groups (n =1 0)based on the cervical collar height(mm):0.0(A),1 .0(B),1 .5(C),2.0(D)and 2.5(E).After veneered with porcelain,all the samples were cemented on the metal tooth analog and then mounted in an universal test machine.The force was applied at a crosshead speed of 1 mm/min until catastrophic fail-ure occurred.Fracture loads and failure modes were recorded.Results:Mean of the fracture strength values(kN)in group A,B,C, D and E were 0.95 ±0.39,1 .29 ±0.50,1 .54 ±0.28,2.04 ±0.1 9 and 2.27 ±0.53 respectively(among groups,P <0.05).Two types of fracture modes were observed:chipping of the veneering porcelain and fracture of the veneering porcelain together with the framework.Conclusion:Zirconia coping design with 1 .5 mm cervical collar height may increase the compressive strength of CAD/CAMzirconia-ceramic restoration.

Objective:This shudy was designed to assess the effects of early enteral nutrition(EEN) via a nasogastric tube(NG)in patients with large cerebral infarction.Methods: Sixty-one patients with large cerebral infarction were enrolled in.The patients were randomly allocated to early enteral tube feeding group and control group for more than two weeks.Mortality,complication,outcome,nutrition condition and grade of nerval action were documented after 3 weeks.Result:The use of early tube feeding via nasogastric tube(NG) was associated with reduction in risk of death and complication and impoved nutrition condition and grade of nerval action.Conclusion:EEN is helpful in patients with large cerebral infarction.

OBJECTIVE: To investigate the perioperative application of antibacterials in our hospital.METHODS: The application of antibacterial drugs in 142 surgery patients sampled randomly from our hospital in 2006 was investigated in respect of the variety of drugs,medication time and drug combination etc.RESULTS: 100% of the total 142 cases received antibacterials for prophylaxis purpose for 6.8 days on average.58 cases(40.8%) received antibacterials before operation,8 cases(5.6%) during operation and 76 cases(53.5%) after operation.35.8% were rational on the whole and 64.2% irrational in drug use.CONCLUSIONS: The application of antibacterials in our hospital is worrisome,therefore,it is urgent to tighten control on the clinical application of antibacterials.