Objective To investigate the risk factors of ischemic stroke in Jiangsu Taixing population.Methods Case-control study was used in this investigation.Cases: all the diagnosed ischemic stroke patients in neurology department of Taixing People 's Hospital from 2011.9 to 2013.6.Controls: all the controls were selected randomly from community residents in Taixing city during the same period of time, according to the age and gender and ratio of 3:2.Univariate analysis and Logistic regression model was applied to analyze risk factors respectively.Results Seven hundred and seventy-seven stroke patients ( case group) and 518 controls ( control group) were brought into the study.It was preliminarily found in univariate analysis that compared with the control group, the rates of smoke, hypertension, diabetes, hyperlipidemia and the waist circumference, waist-to-hip ratio were significantly higher in the case group;while the total bilirubin level was significantly lower ( all P<0.001 ) .Logistic regression showed that smoking (OR=2.089, 95%CI:1.452-3.005), hypertension (OR=3.838, 95%CI:2.516-5.855) were risk factors of stroke in the elder population in Taixing, elevated total bilirubin level ( OR=0.281, 95%CI:0.155-0.508) was protective factor of stroke in the elder population ( all P<0.05 ).Conclusion No smoking, no hypertension history could make it less possible for stroke to occur, and total bilirubin level becomes protective factor of stroke.

Objective To observe the influence of intensive insulin therapy on inflammatory fac-tors and prognosis in severe multiple trauma patients.Methods A total of 53 cases of severe multiple trauma were randomly divided into the treatment group(n =27)and the control group(n =26).Besides basic treatment,patients in the treatment group received additional intensive insulin therapy by micro-pump.The level of blood glucose in the control group was controlled under 11.1 mmol/L.Levels of TNF-α,IL-1β,IL-6,and CRP were tested before and after treatment.Multiple organ dysfunction syndrome,noso-comial infection rate,and mortality rate were also observed.Results The levels of TNF-α,IL-1β,IL-6, and CRP in the treatment group were significantly lower than those of the control group(P <0.05 or P <0.01).The incidence of multiple organ dysfunction syndrome,nosocomial infection,and mortality rate in the treatment group was lower(P <0.05).Conclusion Intensive insulin therapy can effectively decrease the expressions of inflammatory factors in patients with severe multiple trauma,improve the prognosis,re-duce the incidence of nosocomial infection and mortality.

Nursing had a long history over thousands of years which developed from ancient saying better bid the cooks than the medicine,disease-centered and patient centered tohealth-centered clinical nursing.In order to promote quality of clinical nursing,it need innovation by introducing translational medicine into nursing.This paper analyzed how to integrate translational medicine into clinic nursing and explore nursing model based on translational medicine.

Objective To study the clinical and MRI features of central pontine myelinolysis(CPM).Methods The MRI findings of 11 cases with central pontine myelinolysis verified by clinic and imagings were analyzed.Results There were history of drink wine long term in 7 cases,undernourishment in 4 cases,low-blood-sodium in 10 cases.Long T 1 and T 2 signal intensity was showed on MR in central pontine,focus presented under cortex cerebri and in nucleus basalis area in 4 cases.3 cases enhanced around focus,2 cases enhanced in center and 4 cases no enhanced on postcontrast MRI.Conclusion There are characteristic MRI features in central pontine myelinolysis,it is not difficult to diagnoses CPM in early stage if its clinical manifestations and testing treatment with hormone is associated with MRI features.

Objectives To detect the expression level of miR-155 in tissues of colon cancer,and to investigate its influence on the occurrence,development and metastasis of colon cancer.Methods 69 cases with primary colon cancer treated with surgical resection in our hospital were chosen.Tissue samples of the tumor and tissue in the side of tumor(control)were collected and the expression level of miR-155 in the samples was detected by fluorescence quantitative polymerase chain reaction.The results were statically analyzed.Results The expression level of miR-155 was 0.471±0.100,0.238 ±0.108,0.719 ± 0.135 in three groups of colon cancer patients with tumor sizes ≥ 5 cm,high differentiation of tumor,and occurrence of lymphatic metastasis,respectively,and 0.623 ± 0.134,0.142 ±0.094,0.390±0.121 in their controls.And the level of miR-155 was 0.395±0.101 in patients with T1 + T2 tumor local infiltration,0.466±0.128 in T3 + T4 local infiltration,0.404±0.108 in Ⅰ + Ⅱ stage of TNM and 0.457±0.119 in Ⅲ± Ⅳ stage.The miR-155 was relatively higherly expressed in patients with tumor sizes≥5 cm,lower degree of differentiation,higher tumor infiltration degree,Ⅲ and Ⅳ stage of TNM,(t=3.002,12.531,20.425,2.576,1.908,P ＜ 0.05).Conclusions The expression level of miR-155 is correlated with tumor sizes,differentiation degrees,tumor infiltrating degrees,TNM and lymphatic metastasis,which could be used as diagnostic indexes for occurrence,development and metastasis of colon cancer.

OBJECTIVE:To study the pharmacokinetics of tramadol hydrochloride paracetamol tablets in healthy volunteers.METHODS:10 healthy volunteers were assigned to receive oral single dose of tramadol hydrochloride paracetamol tablets.Plasma concentrations of tramadol hydrochloride and paracetamol were measured by HPLC and the pharmacokinetic parameters were computed by 3p97 software.RESULTS:The concentration-time curves of tramadol hydrochloride and paracetamol all fitted one compartment open model.The pharmacokinetic parameters of tramadol hydrochloride and paracetamol were as follows:t1/2Ka were(0.23?0.16)h and(0.64?0.60)h,t1/2ke were(2.12?0.78)h and(5.48?2.90)h,tmax were(0.90?0.43)h and(1.88?1.12)h,Cmax were(9.37?2.56)?g?mL-1 and(115.27?98.88)ng?mL-1,CL/F(s) were(24.52?7.12)L?h-1 and(0.15?0.08)L?h-1,AUC0～t were(30.38?7.47)?g?h?mL-1 and(598.36?232.14)ng?h?mL-1,AUC0～∞ were(33.02?9.15)?g?h?mL-1 and(800.12?420.92)ng?h?mL-1,MRT0～t were(3.75?1.41)h and(5.46?1.64)h,MRT0～∞ were(5.24?2.92)h and(9.86?6.00)h,respectively.CONCLUSION:This study provided a basis for clinical application of tramadol hydrochloride paracetamol tablets.

OBJECTIVE:To study the relative bioavailability and pharmacokinetics of salbutamol sulfate orally disinte?grating tablets.METHODS:A single dose of8mg salbutamol sulfate orally disintegrating tablets or commercial salbutamol tablets was administered in a randomized crossover design in18volunteers and the plasma concentrations of salbutamol were determined by HPLC.The pharmacokinetic parameters were calculated with3p97pharmacokinetic program and the bioequiv?alency was evaluated.RESULTS:The concentration-time curve of two preparations fitted to two-compartment model.The peak plasma levels(C max )of salbutamol sulfate orally disintegrating tablet and commercial salbutamol sulfate tablet were(17.65?6.48)ng/ml and(16.60?6.21)ng/ml,respectively.The peak time(T max )were(1.92?1.18)h and(2.03?1.17)h and AUC 0～24 were(127.23?32.41)ng/(h?ml)and(131.42?37.73)ng/(h?ml),respectively.The relative bioavailability of salbu_ tamol sulfate orally disintegrating tablet was(99.32?15.58)%.CONCLUSION:The results of two one-side tests suggests that salbutamol sulfate orally disintegrating tablet is bioequivalent to the commercial salbutamol tablet.

OBJECTIVE:To study pharmacokinetic parameters of melatonin tablet in beagle dogs.METHODS:A single oral dose of3mg melatonin tablet was given to6beagle dogs,drug concentrations in plasma were determined by HPLC method,the pharmacokinetic parameters were calculated by3P97pharmacokinetic program.RESULTS:The concentration-time curves of melatonin fitted two compartment model in the beagle dogs.The t 1/2Ka 、t 1/2Ke 、t max 、C max 、CL and AUC (0～T) were(0.16?0.10)h、(1.21?0.52)h、(0.50?0.18)h、(11.27?3.77)ng/ml、(0.13?0.04)L/h and(25.23?7.71)(ng?h)/ml re-spectively.CONCLUSION:It is rapid to absorb melatonin and initiate sleep in beagle dogs,but it's hold-time is short.

Since the discovery of mast cells (MCs) in 1878, MCs have long been recognised to be the key effector cells in allergic reactions. However, little is known about their roles in defending microorganisms. In this review, the recent research progress in the roles of MCs, in the body defence against bacterial and viral infection, in pathogen recognition mechanisms and in inflammatory mediator release are discussed.