Objective:To investigate the proper method for treating traumatic chyloperitoneum. Methods: A retrospective analysis was performed on clinical data of 4 cases of traumatic chyloperi- toneum admitted to our hospital in recent 26 years. Results: All 4 cases were cured after conserva- tive treatment. Conclusion: Perdominantly integrated therapy including absolute diet,total parrenter- al nutrition(TPN) and abdominal cavity drainage can be the effective method for the treatment of trau- matic chyloperitoneum.

Objective To investigate the biological effects of nonthermal plasma (NTP) combined with X-ray irradiation on human hepatocellular carcinoma in nude mice.Methods By subcutaneous inoculation, tumor models of human hepatocellular carcinoma HepG2 in nude mice were established, and these mice were divided into control group, NTP treatment group, X-ray irradiation group, combined treatment group.The changes of tumor microenvironment were observed using hematoxylin-eosin (HE) staining.The terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) was used to determine the level of neuronal apoptosis in tumor tissues.The expression of metal matrix proteinase-2 (MMP-2) was detected by immunohistochemical assay.Transmission electron microscope was used to observe cell structure changes.Results The final tumor volumes of control group, NTP treatment group, X-ray irradiation group, combined treatment group were (543.96±108.45), (436.54±65.49), (351.66±56.68), (281.97±35.60)mm3, with a statistically significant difference (F=9.63, P=0.01), and the difference between X-ray radiation group and the combination treatment group was significant (P=0.05).HE staining showed that there was a larger area of necrosis in the combined treatment group compared with the other groups.TUNEL showed that the apoptotic indexes were (0.95±0.13)%, (5.82±0.26)%, (7.53±0.43)%, (11.07±0.35)% respectively, with a statistically significant difference (F=547.76, P=0.00), and the difference between X-ray radiation group and the combination treatment group was statistically significant (P=0.00).The immune scores of each group were 12, 9, 9, 2.Electron microscopic observation showed that there were more apoptotic bodies in the combined treatment group than those in the other groups, accompanied by mitochondrial edema.Conclusion NTP and X-ray irradiation therapy in the treatment of human hepatocellular carcinoma in nude mice model has a synergistic effect.Probably, it can be a new type of treatment in curing cancers.

Knowledge management, as a new managerial concept, is the specific embodiment of knowledge based economy in the field of management while technological innovation, constituting the core of technological progress, is the effective extension and application of scientific and technological knowledge. Hospitals, which are knowledge based institutions, possess good foundations and advantages of implementing knowledge management and technological innovation. There is a close link between knowledge management and technological innovation in hospitals, the former being the basis and primary guarantee of the latter and the latter being the result and manifestation of the former. Hospital managers should take measures to promote the sound interaction between the two so as to enhance the core competency of their hospitals.

Inspect the anchorage molars with band and the anchorage molars with buccal tube with dental plaque index and gum in- dex and then statistically process the objective data obtained.The results show that both wearing band and wearing buccal tube lead to dental plaque.The influence is less worse on the the anchorage molars with buccal tube.

HPLC was applied to content determination of active components——chlorogenic acid and baicalin in Qingkailing Injection.This method is simple,rapid,involves good reproducibility,and can be as a reliable determination method for the quality control of Qingkailing Injection.The test conditions are as follows:detection wavelength at 340 nm,ODS C-18 chromatocolumn of stainless steel(3.9?30cm),mobile phase using methanol-water-tetrahydrofuran(25:57:18).

Objective To investigate the expression of aquaporin-1 (AQP1) in human peritoneum and its effect on peritoneal dialysis (PD) patients with the different peritoneal transport characteristics.Methods Peritoneal biopsies were obtained from the PD patients (n =30) at catheter insertion.Western blot and immunohistochemical staining were used to investigate AQP1 expression in peritoneal tissues.After catheter insertion,4 week,peritoneal equilibrium test (PET) was used to decide peritoneal transport characteristics.Results Western blot revealed a major band at 28 kDa band.The expression of AQP1 index was 1.49 ±0.67 in the normal subjects,and 1.61 ±0.76 in the PD patients,and there was no significant difference.Positive deposition was distributed in mesothelial cells,endothelial cells of capillaries,and small veins.The index of AQP1 expression in endothelial cells of capillaries in normal subjects and PD patients were 2.12 ±0.18,and 2.50 ±0.81,respectively,and no significant difference was found.The index of AQP1 expression in mesothelial cells of capillaries in normal subjects and PD patients were 1.95 ± 0.67,and 2.23 ± 1.07,respectively,and no significant difference was found.Among PD patients,the level of AQP1 was significantly different.Conclusions The expression of AQP1 was different in the PD patients with different peritoneal transport characteristics.In the high and high average transport groups,the level of AQP1 was lower; the low transport group was higher.It reveals that AQP1 plays a role in the pathogenesis of transcellular water transport.

Objective To evaluate the clinical significance of K-ras mutation in plasma cell-free DNA from patients with colorec-tal carcinoma .Methods Plasma cell-free DNA in 212 patients with colorectal carcinoma were extracted and identified by Globin gene .K-ras mutation was amplified by RE-PCR and compared with results of pathological biopsy samples DNA .Results Plasma cell-free DNA were successfully obtained from 212 cases .But no plasma cell-free DNA were found in normal control .Out of 212 ca-ses ,34 cases showed K-ras mutation(16 .00% )in cell-free DNA ,and 33 cases showed positive in pathological biopsy samples DNA (15 .57% ) .It was found two kinds of K-ras mutation on code 12-GGT to GAT ;GGT to GTT .K-ras mutation was not related to tumors′position(χ2 =3 .34 ,P=0 .61);to differentiation degree(χ2 =2 .38 ,P=0 .29);to Dukes staging(χ2 = 2 .84 ,P=0 .38);to lymphoid nodes′metastasis(χ2 =0 .98 ,P=0 .37);to age(χ2 =1 .86 ,P=0 .39) .Conclusion Tumor-derived DNA can be detected in plasma of underlying cancer patients .For colorectal carcinoma patients ,detecting K-ras mutation of plasma cell-free DNA have the same clinical significance as biopsy samples .Moreover ,it′s more convenient and non-invasive .

Objective To analysis the rational use of human albumin in patients with hypoproteinemia in our hospital.Methods The drug evaluation criteria of human albumin were developed and the patients of different gen-dem,ages,departments,clinical diagnoses,human albumin species,administration,doses,course of treatment and pur-poses in our hospital were investigated.Results The using rate of human albumin in patients with hypoproteinemia was 53.1% in our hospital,20% human albumin accounted for the most(89cases),and the consumption of human albumin used in department of respiration was the most in our hospital (70cases).Diagnosis of 14 cases were not anastomosis with indications and 8 cases hadprolonged use and no indicative use and inappropriate medication. Conclusion The irrational use of human albumin are existed in our hospita1.We should develop the drug using standard of human albumin and strictly grasp the indications of drug use to avoid overuse of human albumin.

Objective To investigate the efficacy of flupentixol and melitracen combined with pinaverium bromide in the treatment of patients with irritable bowel syndrome. Methods A total of 64 patients with irritable bowel syndrome from October 2014 to October 2015 in department of gastroenterology of Zhuji People’s Hospital were collected and randomly divided into two groups, with 32 cases in each group.Patients in control group received with pinaverium bromide by oral, patients in combination group received flupentixol and melitracen by oral on the basis of control group.The gastrointestinal symptom rating scale (GSRS), self-rating anxiety scale (SAS), self-rating depression scale (SDS) score and clinical efficacy pre-and post-treatment were compared between two groups.Results Compared with pre-treatment, the main symptoms score in two groups decreased significantly (P<0.05); compared with control group, the main symptoms score in combination group decreased significantly(P <0.05).Compared with pre-treatment, the SAS and SDS scores decreased significantly(P <0.05); compared with control group, the SAS and SDS scores in combination group decreased significantly ( P <0.05 ) .There was no significant difference in total efficacy between control group and combination group ( 93.75%vs.81.25%, χ2 =1.237,P=0.266).There was no adverse event in two groups during treatment.Conclusion Flupentixol and melitracen combined with pinaverium bromide could significantly improve the anxiety and depression in the treatment of irritable bowel syndrome, which could promote the recovery of gastrointestinal function.

[Purpose] Study measures and solutions to students ’employment problem. [Methods] Adopt basic research methods such as literature review, in-dividual interview, data analysis, etc. [Result] Students ’employment situation is grim in Traditional Chinese Medicine col eges and universities, the em-ployment work needs to be enhanced in many aspects. [conclusion] Traditional Chinese Medicine col eges and universities should improve the quality of employment work through strengthening students’competition ability and optimizing the structure of talent training.