Objective To explore the pattern of spontaneous neural activity in different frequency bands in Parkinson's disease(PD).Methods The blood oxygen level-dependent signals in 39 PD patients and 37 normal controls were acquired and the amplitude of low-frequency fluctuation ( ALFF) in five differ-ent frequecy bands ( slow-2:0.198-0.25 Hz;slow-3:0.073-0.198 Hz;slow-4:0.027-0.073 Hz;slow-5:0.01-0.027 Hz;slow-6:0-0.01Hz) was cacaulated.A repeated-measures analysis of variance and Post hoc t-test were performed to detect differences of spontaneous activity between two groups in different frequency bands. A correlation analysis was examined between Unified Parkinson’ s Disease Rating Scale ( UPDRS) motor score and ALFF in patients with PD in each frequency band to discover the relationship between disease se-verity and spontaneous neural activity.Results Compared with the normal controls,patients with PD had decreased ALFF in the putamen(-24,-3,-9) (-30,3,0) ( -21,9,6) in the slow-3,slow-4 and slow-5, and had decreased ALFF in the left precentral gyrus (-21,-24,75) (-21,-24,72) (-27,-21,72) (-15,-15,72) and right supplementary motor area (21,-24,75) (6,-3,72) (12,-6,60) (15,-24,72) in all frequency bands except for the slow-5.Increased ALFF in the cerebellum was found in all frequency bands.The ALFF in the putamen was negatively correlated with the UPDRS motor score in the slow-3,slow-5 and slow-6.Conclusion This study shows that the abnormal activities in the striatum are significant in the slow-3,slow-4and slow-5.Most frequency bands except slow-5 are helpful in detecting neural changes in mo-tor cortex.The increased activity in the cerebellum is the common findings in all frequency bands.Multiple frequency analysis may provide insight into the pathophysiological mechanisms of PD.