1.Application of graphic method in external quality assessment of iodized salt
Chinese Journal of Endemiology 2015;34(10):773-776
Objective To evaluate the detection capability for salt iodine test by using graphic method,and to provide reliable detection data for prevention of iodine deficiency disorders.Methods The examination results of external quality assessment for disease control mechanism in Liaoning Province in 2013 were analyzed by using standard Z score and Youden plot.The level of reproduction and type of error were analyzed by Z score sequence diagram in order to find the outliers and results need to be reviewed by the Youden plot.Salt iodine was detected by the general test method in salt industry for determination of iodine (GB/T 13025.7-2012).Results According to single sample evaluation of the Z score,111 laboratories were qualified,the qualified rate was 97.4% (111/114).Three labs were not qualified,unqualified rate was 2.6% (3/114).According to double sample evaluation of the Z score,112 laboratories were qualified,the qualified rate was 98.2% (112/114).Two laboratories were not qualified,unqualified rate was 1.8% (2/114).According to the Youden plot,5 laboratories were possible outliers with results need to be reviewed.Conclusion Statistical graphic method has displayed the examination results directly,which is helpful in searching targeted improvements,and improving the detection capability.
2.Clinical observation on biifco combined with triple therapy for eradication of Helicobacter pylori
Chinese Journal of Biochemical Pharmaceutics 2014;(2):137-138
Objective To investigate the clinical efifcacy of biifco combined with triple therapy for eradication of Helicobacter pylori. Methods 84 cases with Helicobacter pylori infection were selected as the observation objects, and were randomly divided into treatment group and control group, 42 cases in each group. Control group used standard triple (omeprazole, amoxicillin, clarithromycin) oral;treatment group took biifco on the basis of control group use. Hp eradication rate, gastrointestinal symptoms and the incidence of adverse reaction in two groups after treatment were compared. Results Hp eradication rate in treatment group was 92.9%, higher than 75.0%in control group, the difference was statistically signiifcant(P<0.05);the incidence of adverse reaction in treatment group was 2.4%, signiifcantly lower than 10.0%in control group, the difference was statistically signiifcant (P<0.05);the total symptom relief rate in treatment group was 90.5%, signiifcantly higher than 75.0%in control group, the difference was statistically signiifcant(P<0.05). Conclusion The eradication rate of biifco combined with triple therapy for Helicobacter pylori is high and signiifcant, which can effectively reduce adverse gastrointestinal reactions.
3.N-acetylcysteine attenuates myocardial hypertrophy in streptozotocin-induced diabetic rats
Yuhong HE ; Zhongyuan XIA ; Fang WANG
Chinese Journal of Diabetes 2010;18(3):211-213
Objective To investigate the protective effect of N-acetylcysteine(NAC) on the development of myocardial hypertrophy in diabetic rats induced by streptozotocin. Methods Male SD rats were randomly divided into normal control (NC), control treatment (NCT), diabetic (DM) and diabetes treatment (DMT) groups. NAC was administered at dose of 1.4~1.5g·kg~(-1)·d~(-1) to NCT and DMT groups in the drinking water for 8 weeks. At termination the rats were surgically prepared for hemodynamic measurement, such as systolic blood pressure, heart rate, the rate of left ventricle relaxation (-dp/dt) and the time to maximum relaxation (T). Subsequently, the hearts were removed to assay relative ratio of left ventricle weight versus body weight (LVW/BW) and cardiomyocyte cross sectional areas.Plasma glucose, insulin, 15-F_(2t)-isoprostane(15-F_(2t)-Isop) concentration and superoxide dismutase (SOD) activity were analyzed. Results The LVW/BW ratio and cardiomyocyte areas were significantly attenuated by NAC treatment as compared to DM group (P< 0.05). Increases in -dp/dt and SOD activity and the decreases in glucose, 15-F_(2t)-Isop T (P< 0.05) were found at the same time. Conclusions NAC can effectively prevent the development of myocardial hypertrophy and improve diastolic function in diabetic rats, which may be related to its antioxidant capacity.
4.Mechanism of protective effect of sodium nitroprusside against endotoxin- induced acute lung injury in rats
Zhongyuan XIA ; Xiaoyuan WANG ; Daping DU
Chinese Journal of Anesthesiology 1995;0(02):-
Objective To investigate the effect of sodium nitroprusside (SNP) , an exogenous NO donor, on expression of hemeoxygenase-1 (HO-1) and inducible NO synthase (iNOS) in the lung tissue in a rat model of lpopolysaccharide (LPS)-induced acute lung injury and the underlying mechanism.Methods Thirty-two Wistar rats of either sex weighing 190-210 g were randomly divided into 4 groups ( n = 8 each) : Ⅰ sham operation (S) ;Ⅱ LPS;Ⅲ hemin + LPS and Ⅳ SNP + LPS. The animals were anesthetized with intraperitoneal (IP) 7% chloralhydrate 5 ml?kg-1. LPS 750 ?g?kg-1 in 300 ?l was instilled into the lungs via trachea in group Ⅱ . In group Ⅲ hemin (a HO-1 inducer) 40 ?mol?kg-1 was injected IP 12 h before LPS. In group Ⅳ SNP 25 ?g?kg-1 + LPS 750 ?g?kg-1 in 300 ?l was instilled into the lungs via trachea. In group S normal saline 300 ?l was instilled via trachea instead of LPS. The animals were killed by (1) exsanguination from carotid artery 8h (2) after LPS instillation and lungs were removed for broncho-alveolar lavage (BAL) and detennination of protein concentration in BALF and the expression of HO-1 and iNOS protein in the lung tissue by immune-histochemical technique and HPIAS-2000 image analysis system and MDA content in lung tissue and (3) light microscopic examination (4) wet/ dry lung weight ratio.Results The 4 groups were comparable with respect to body weight and sex (M/F ratio) . The expression of iNOS and HO-1 in lung tissue was significantly higher in LPS group than in S group ( P
5.Progress in mesenchymal stem cells as a novel nano-targeted antitumor drug carrier
Zhongyuan WANG ; Yanping WU ; Lin MEI
International Journal of Biomedical Engineering 2013;36(3):129-133,后插2
In recent years,targeted drug delivery system as one of the hottest areas in pharmaceutical research has received more and more attention.However,the targeted anti-tumor drug delivery system has many shortcomings including low targeted efficiency which limit its clinical application.Elevating tumor targeting efficiency has become the bottleneck of nano-drug development and clinical application.Therefore,it is urgent to design and develop novel targeted drug delivery vehicle.Recent studies showed that mesenchymal stem cells (MSCs) have characters of tumor tropism and migration,which make them as ideal targeted anti-tumor drug delivery carriers for the treatment of solid and metastatic tumors.This paper reviews the progress in using MSCs as targeted anti-tumor drug delivery carrier,recapitulates the problems and challenges of the system,and proposes a solution for these problems.
6.Effect of radix paeoniae rubra on expression of heme oxygenase and inducible nitric oxide synthesis in lipopolysaccharide-induced acute lung injury in rats
Zhongyuan XIA ; Chang CHEN ; Xiaoyuan WANG
Chinese Journal of Trauma 2003;0(09):-
Objective To investigate the effect of radix paeoniae rubra (RPR) on expression of heme oxygenase (HO-1) and inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-induced acute lung injury (ALI) and explore its protective mechanism. Methods Forty Wistar rats were randomly and equally divided into five groups, ie, control group, LPS group, RPR treatment group, RPR prevention group and Hemin group. Arterial blood was drawn for blood gas analysis. Models of endotoxin-induced ALI were used to observe the protein content, the ratio of neutrophiles in bronchoalveolar lavage fluid (BALF), the malondialdehyde (MDA) content in lung and the activities of serum NO. Expression of HO-1 and iNOS in rat lung tissue was detected by immunohistochemistry and morphometry computer image analysis. The histological change of lung were observed under light microscope. Results Compared with control group, expression of HO-1 and iNOS was markedly increased (P
7.Expression,purification and characterization of recombinant multiepitope peptide of ESAT-6 of Mycobacterium tuberculosis
Haibo WANG ; Zhongyuan ZHU ; Xianjie LIU
Chinese Journal of Clinical Laboratory Science 2006;0(03):-
Objective To obtain sufficient recombinant multiepitope peptide of ESAT-6.Methods DNA encoding ESAT-6 peptide was amplified by PCR using a pair of primers which were designed in accordance with the reported ESAT-6 gene sequence.The ESAT-6P gene was inserted into the prokaryotic expression vector pET-30a with the N-terminal 6His-tag.The recombinant peptide vector pET30a-ESAT-6P was transformed into E.coli BL21(DE3) via chemical transformation.The positive clone was screened by means of PCR.The target peptide was expressed in E.coli after induction with IPTG and analyzed by SDS-PAGE.The immunogenicity of the peptide was evaluated by dot immunobinding assay.Results The target peptide was successfully expressed and purified.The solubility analysis showed that the recombinant peptide existed as inclusion body in the E.coli.DIBA indicated that the ESAT-6P was specifically reactive to sera from TB patients.Conclusions The recombinant multiepitope peptide of ESAT-6 engineering bacteria has been obtained,which will be quite helpful to develop novel specific diagnostic reagents and effective vaccine.
9.THE DEVELOPMENT OF COCHLEAR DUCT AND ORGAN OF CORTI IN HUMAN FETUSES
Shuhong DAI ; Zhongyuan WANG ; Yinzi WANG ; Xiaofeng CHANG ; Li LIU
Acta Anatomica Sinica 1954;0(02):-
The authors observed the development of the organ of Corti in the temporal bones of 64 human fetuses of various fetal ages by means of comparing celloid sections with surface preparations. The organ of Corti just begins to appear in the second month. Most rapid development occurs in the period from the third to the fifth month. Afterwards, the principal structure of the organ of Corti tends to be stable with no further major changes, except for the differentiation and maturation of the intracellular components. In the fifth month, the major part of the higher columnar epithelial cells on the inner Side of the tunnel begins to degenerate and disintegrate before the formation of the inner spiral sulcus. But a few epithelial cells turn into large wandering cells. The authors discussed the characteristics, function and possible transformation of the wandering cells and compared them with essentially similar cells that appear in the developing central nervous system.
10.BUILDING AND MANAGEMENT OF BANK OF MYCOBACTERIUM AND THEIR GENOME
Hongmin LI ; Zhongyuan WANG ; Wei WANG ; Zhihong LI ; Zhen LIU ;
Microbiology 1992;0(04):-
With the development of technology,automatic management system Controlled by co mputer is necessary in hospitals Building a bank of mycobacterium and their gen ome is a great step to this aim It can make it easier to get the mycobacterium data than before,furthermore,it is helpful for further research for doctors to m ake a new treatment plan for the patients