‘Stasis-heat’is a pathological factor that arises from exogenous and endogenous injuries in the development and progression of diseases.It can also be the pathogenesis at a certain stage of disease.It manifests clinically as the syndrome of‘Stasis-heat Interactivity.’Through long-term clinical practice‘Stasis-heat’has been found to spread in a variety of diseases’processes and has general clinical significance.Using‘Stasis-heat’theory in prescribing an herbal formula can significantly improve clinical efficacy,displaying the advantages of using traditional Chinese medicine to prevent and treat acute and severe diseases.According to more than 30 years of research and clinical practice,based on ongoing discussion and revision by experts,we have developed the‘Guide to TCM Syndrome Differentiation and Treatment of Stasis-heat Interactivity Syndrome,’a work that is an essential reference for both Chinese medicine and integrated Chinese and Western medicine clinicians.This article lays the groundwork in the hope that other experts will provide further input that will improve the guidelines laid out in this piece of work.

Objective To study the security and effectiveness of leg lengthening in increasing the height of short stature for cosmetic reasons. Methods The improved bone lengthening technique was used to 60 adult short stature volunteers to prolong bilateral lower legs. All data, including healing time of the distracted bone, operative complications, recovery of limb function and height increment, were recorded. Results In all cases, the height increment was 5 to 10 cm (mean 6.8cm). Bone lengthening rate was 19.6 %. The healing time of the distracted bone was 6 to 16 months (mean 8.2 months). There was no severe complication occurred. Follow up for 2 to 8 years showed that all cases returned to work or study with normal gaits and satisfied body figure. Previous psychologic obstacles were eliminated. Two years after bone healing, the ability for intense activities was regained. Conclusion Leg lengthening for cosmetic heightening is a safe and effective therapeutic approach.

Objective:From a professional competency-based perspective,this study aimed to explore appro-priate ways for certifying counselors and psychotherapists in China,and focused on improving the evaluation pro-gram for clinical and counseling psychologist of the Clinical and Counseling Psychology Registration System (CCPRS)of Chinese Psychological Society.Method:The theories and methods for certifying counselors and psy-chotherapists in China and overseas were summarized by using the literature method,based on which the design principles for certification in China were determined.Using the expert method,the core competencies of Chinese counselor and psychotherapist were proposed to form the index system of evaluation.A survey was also conducted to obtain a preliminary validity of the improved evaluation program.Result:Design principles for certifying coun-selors and psychotherapists were multi-trait,multi-method,and multi-informant.The core competencies of Chinese counselor and therapist included six domains,which were professional attitudes and conduct,ethics and law,clinical knowledge and skills,science and research,relationship building,cultural diversity and Chinese culture,case man-agement,respectively.Results from the expert judgments indicated that the evaluation materials which formulated by the improved program could assess all six core competencies and had acceptable reliabilities(Cronbach's α=0.74-0.97).And the expert evaluation about the improved program was high.Conclusion:The core competencies of Chinese counselor and therapist,which formulated by the improved evaluation program,reflect the necessary attitudes,knowledge and skills that needed when engaged in the professional practice of counseling and psychothera-py,and also can be used as a reference for counselor and psychotherapist education,training,practice,and compe-tency evaluation in China.The improved evaluation program can be used as a transitional way for certifying coun-selors and psychotherapists in China.

Objective To investigate the clinical significance of detecting mammaglobin in sentinel lymph nodes(SLN) of breast cancer. Methods In 32 breast cancer patients, methylene blue was injected around parechyma of breast cancer to locate the SLNs, and nested RT PCR(reverse transcriptase polgmecane chain reaction) was used to examine the expression of mammaglobin mRNA. Results The SLN was successfully identified in 30 of the 32 cases(93.8%).The micrometastases detection between group SLNs and non SLNs had significant statistical difference(P

Objective To research the clinical outcome of atypical glandular cell (AGC)according to various subtypes of HPV infection and histological pathology results.Methods The data of the liquid-bases cytology (LBP),HPV infection and histology in 102 cases of AGC at the gynecology outpatient department of our hospital from January 1 ,2009 to February 28,2014 were collected and performed the analysis on their clinical outcomes.Results Among 67 218 cases of LBP detection,102 cases were AGC with the total incidence rate of 0.15%.In the cases of AGC-NOS,67 cases were normal or benign lesions,11 cases were precancerous lesion and malignant lesions;in the cases of AGC treading to tumor,the benign,precancerous and malignant lesions were in 7,14 and 3 ca-ses respectively.At the same time in the cases of AGC-NOS,HPV infection was in 64 cases,in which 57 cases were high risk infec-tion(type 16,52,45)and 7 cases were low risk infection(type 6,11 ).The single infection,double infection and multiple infection were in 54,6 cases respectively;in the cases of AGC trends to neoplasm,HPV infection was in 19 cases,in which 18 cases were high risk infection(type 52,16,18)and 1 case was low risk infection(type 6),single infection and double infection were in 15 cases and 4 cases respectively.Conclusion AGC may play an important role for the forecast of cervical malignant lesions.The results of differ-ent HPV subtypes infection in AGC related tumors also play a certain role in the prediction of cervical neoplasia.Their combined a-nalysis is the important signal for judging the occurrence of gynecological cervical precancerous lesion and malignant tumor,i.e., AGC combining with the corresponding HPV subtype infection not only can make a judgement for the cervical lesions,but also pro-vides the basis for predicting the high risk existence of gynecological malignant tumor and provides constructive suggestions for Chi-na regional cervical carcinoma vaccine manufacturing and promotion.

Objective With the purpose of further improving blood safety and providing the reference basis for a strategy suitable for large-scale routine screening of irregular antibodies in blood service,the irregular antibodies in blood samples from donors in Shanghai area were investigated.Method Irregular blood antibodies were screened in 824 072 blood donors through combined method of saline medium and microplate papain.Result 1 246 samples of blood donors were detected with irregular antibodies among the total 824 072 samples which indicate the positive rate of 1.51‰.Conclusion Screening of irregular antibodies in blood samples from donors can effectively reduce or avoid hemolytic transfusion reactions.The combined method of saline medium and microplate papain is an effective method for large-scale routine screening of irregular antibodies in blood service.

Objective To study the methods andfeasibility of re-entry of NAT reactive blood donors.Methods ELISA Non-Reactive(NR) and NAT Reactive(R) Blood Donors in 2012-2014 were selected and informed consent.Blood samples collected were tested:ELISA (HBsAg、Anti-HCV、Anti-HIV and Anti-TP) and NAT (HBV-DNA、HCV-RNA and HIVRNA).ELISA NR/NAT NR samples were tested by ECLA for HBsAg、HBeAg、Anti-HBs、Anti-HBc、Anti-HBe、Anti-HCV、Anti-HIV.Results 30 donors were called back,and 11 of them were ELISA NR/NAT R,which were deferred permanently.19 of them were ELISA NR/NAT NR,and the ECLA results were 89.5％ ELISA NR/NAT NR donors were Anti-HBc R and/or Anti-HBe R (12 cases Anti-HBc/Anti-HBe R、5 cases Anti-HBe R).Anti-HBc R or Anti-HBe R donors had risk of infection,which were deferred permanently.2 cases all items Non-Reactive may be eligible for re-entry.Conclusion Re-entry of NAT reactive blood donors is very necessary and meaningful,but in the view of blood safety,it is important that establish a set of safe program for Chinese.

Objective To describe the status of prevalence,awareness,treatment,and control of hypertension among the residents in severe historical endemic areas of Keshan Disease in Heilongjiang Province,and to provide scientific evidence for prevention and control of hypertension.Methods In December 2015,Yongjin,Yongli groups of Yongjin Village,and Fengnian,Fengtian,Fengfu groups of Fengnian Village in Fanrong Township,Fuyu County,Qiqihaer City,Heilongjiang Province were selected via the cross-sectional method.The residents aged 18 years or older were surveyed as respondents by cluster sampling.Demography,risk factors,hypertension related information were collected through questionnaire survey.Blood pressure,height and weight were measured through physical examination.Blood pressure measurement was conducted with Omron HEM-1020 electronic sphygmomanometer,the diagnostic criteria was Chinese Guidelines for Prevention and Treatment of Hypertension (revised in 2010).Results Totally 683 residents were surveyed,the valid participants were 669,the prevalence of hypertension was 49.2％ (329/669),the standardized prevalence was 37.9％,the prevalence of men [58.0％ (164/283)] was higher than that of women [42.7％ (165/386),x2 =15.103,P ＜ 0.01],the prevalence increased with increasing age (total,men,women:x2tendency =9.044,4.560,7.789,all P ＜ 0.01).The awareness of hypertensive patients was 60.8％ (200/329),the standardized awareness was 41.2％,the awareness of men [54.3％ (89/164)] was lower than that of women [67.3％ (111/165),x2 =5.836,P ＜ 0.05],the awareness of hypertensive patients increased with increasing age (total,men,women:x2tendency =3.638,2.686,2.419,all P ＜ 0.05).The treatment of hypertensive patients was 51.1％ (168/329),the treatment of men [43.9％ (72/164)] was lower than that of women [58.2％ (96/165),x2=6.711,P ＜ 0.05],the treatment of hypertensive patients increased with increasing age (total,men,women:x2tendency =4.422,2.704,3.633,all P ＜ 0.01).The control rate of hypertensive patients was 5.5％ (18/329),the control rate among the treatment was 10.7％ (18/168).Conclusions The standardized prevalence of hypertension among the residents of the severe historical endemic areas of Keshan disease in Heilongjiang Province is higher than the national average (25.2％),the standardized awareness is lower than the national average (46.5％),the treatment and the control are low.We should pay more attention to the hypertension in the severe historical endemic areas of Keshan disease.

Previous studies suggest that NGAL (neutro phil gelatinase-associated lipocalin) is involved in the transformation and development of esophageal carcinoma. Alteration of NGAL expression can trigger the change of cellular morphology in esophageal carcinoma cells. However, the mechanisms remain unclear. To get a better understanding of NGAL function in esophageal carcinoma, NGAL protein was expressed in methylotrophic yeast, Pichia pastoris, and purified by chromatography. EC1.71 cells expressed high levels of NGALR (NGAL receptor) and EC109 cells expressed low levels of NGALR were used as cells model. The trafficking and the possible function of NGAL protein were then analyzed in the esophageal carcinoma cells. The results showed that 5-FAM-labeled recombinant NGAL protein could internalize into the EC1.71 and EC109 cells. Furthermore, the internalized NGAL protein could induce the alteration of cellular morphology, resulting in generation of autophagosome, transcriptional up-regulation of genes associated with autophagy and increase of phospho-ERK1/2 (p-ERK1/2). Interestingly, the treatment with the NGAL protein did not affect the intracellular iron level. These data indicate that induced autophagy by exogenous NGAL protein is a mechanism that internalized NGAL plays important roles in esophageal carcinoma cells, independent with NGAL-mediated iron transport process, while ERK1/2 signal pathway is involved in activation of autophagy by exogenous NGAL protein.

Objective To investigate the potential of polylactic acid glycolic acid copolymer (PLGA) composite ordered multi tunnel collagen scaffold in fabricating a biomimetic artificial nerve graft to repair the sciatic nerve defects in rats.Methods The ordered multi tunnel collagen scaffold was prepared by vacuum freeze-drying and directional drawing method to simulate the epineurium;the outer conduit was prepared by PLGA to simulate the epineurium;and then,the ordered multi tunnel collagen scaffolds were loaded in the PLGA conduit (5∶1) under a stereomicroscope to develop a novel biomimetic artificial nerve.Sixty-four rats were randomly divided into four groups:artificial nerve group,PLGA group,peripheral nerve group,and non-graft control group (n=16);rats in the artificial nerve group,PLGA group,and peripheral nerve group were repaired with artificial nerve graft,hollow PLGA conduit and allogeneic sciatic nerve to bridge the sciatic nerve defect,while the sciatic nerve with the gap in rats of the control group was without any grafting.After 11 weeks of operation,the hind limbs of rats in each group were detected by behavioral test,electrophysiological examination and Fluoro-Gold retrograde tracing method.The changes of muscle tissues (gastrocnemius) were observed by hematoxylin staining and TMR-α-BTX staining,and the regenerated axons were observed by immunohistochemical staining with NF200 and the regenerative spinal anterior horn motor neurons were observed by Nissl fluorescence staining 12 weeks after operation.Results After 11 weeks of operation,the recoveries of the motor functions (the distance between the injured hindlimb and forelimb,the rotation angle of the injured foot) in the peripheral nerve group,artificial nerve group,PLGA group and control group were significantly deteriorated in turn,and the differences were statistically significant (P＜0.05).Electrophysiological examination showed that the recovery effect of peripheral nerve group was the best in both latency and amplitude of the compound muscle action potential,followed by artificial nerve group.The latency of PLGA group was the longest and the amplitude of compound action potential was the smallest;significant differences were noted between each two groups (P2＜0.05).At 12 weeks after operation,the wet weight ratio of muscle fibers,area of muscle fibers and neuromuscular junction area were significantly different between each two groups (P＜0.05);the degree of gastrocnemius atrophy in the artificial nerve group was significantly improved than that in the PLGA group,but not yet reached the level of peripheral nerve group.NF200 immunohistochemical staining showed that a large number of NF200-positive axons were seen in the grafts of the artificial nerve group,but the number was slightly smaller than that of the peripheral nerve group;the number of regenerated axons in the PLGA group was the smaller and mainly distributed near the proximal side.In the PLGA group,only (19.33 ±6.73)％ regenerated spinal anterior horn motor neurons were labeled with Fluoro-Gold,while the positive rates of Fluoro-Gold in the artificial nerve group and peripheral nerve group were (42.67±7.45)％ and (50.13±4.33)％;the differences between each two groups were statistically significant (P＜0.05).Conclusion The biomimetic artificial nerve made of PLGA conduit and ordered multi tunnel collagen scaffold can efficiently reconstruct the defected peripheral nerve with guiding axonal regeneration and promoting functional restoration in rats;however,its effect is poor than peripheral nerve grafting.