1.Evaluation of MODS for susceptibility testing of second-line drug in Mycobacterium tuberculosis
Youhui FU ; Ruijuan ZHENG ; Jie WANG ; Wenguo JIN ; Zhongyi HU
Chinese Journal of Laboratory Medicine 2010;33(1):25-29
Objective To test the drug susceptibilities in Mycobacterium tuberculosis by microscopic observation drug susceptibility(MODS)and evaluate the method for the detection susceptibility in second-line drugs.Methods To set up the MODS method.the drug susceptibilities of 4 second-line drugs in Mycobacterium tuberculosis were tested in 24 well plates.The test conditions were discussed.The resistance to protionamide(PTH),amikacin(AMK),capreomycin(CPM)and levofloxacin(LVF)of 60 MTB isolated from 2007 to 2008 in Shanghai Pulmonary Hospital were detected and compared with that of L-J method.The isolates were tested by minimum inhibitory concentration(MIC)method when the drug susceptibilities were not consistent.Results Among the 60 Mycobacterium tuberculosis clinical isolates.the results of drug susceptibility were confirmed by MODS,absolute concentration method and the accordance rate of PTH,AMK,CPM and LVF were 96.7%(58/60),98.3%(59/60),91.7%(55/60)and 96.7%(58/60),respectively.If the result of absolute concentration method was as the standard.the sensitivity.specificity.positive and negative predictive value as well as accuracy were 100%,87.5%,87.5%,100%and 96.7%in PTH;100%,90.0%,90.0%,100%and 98.3% in AMK;76.9%,95.7%,83.3%,93.8%,91.7% in CPM;100%,96.0%,83.3%,100% and 96.7% in LVF respectively detected by MODS assay.Conclusion MODS is a rapid and simple method for susceptibility testing of second-line drug in Mycobacterium tuberculosis.
2.Effect of aerobic exercise on genome expression in human skeletal muscle
Biyan HUANG ; Jiebing KE ; Xiang ZHANG ; Zhongyi FU ; Li JIANG ; Yi SUN ; Wang WANG
Chinese Journal of Tissue Engineering Research 2009;13(28):5580-5584
OBJECTIVE: To evaluate the effect of aerobic exercise on genome expression in human skeletal muscle. METHODS: Six healthy sedentary elderly men aged (66+9) years were selected from military cadre retirement centers and after exercise. Testing indices included height, weight, vital capacity, step index and maximal oxygen uptake. Needle biopsies were obtained from the skeletal muscle before and after the last training. Total RNA extracted from the samples was hybridized to Affymetrix U 133A platform, the gene expression datum was analyzed.RESULTS: Aerobic exercise was shown to improve cardiorespiratory function and reduce body fat of elder subjects. It could alter the genome expression in human skeletal muscle, the number of genes that passed filtering criteria was 725. The most differently expressed genes (n=20) were investigated in this study, in which there were 3 upregulated and 17 downregulated. According to gene function annotations, the differential genes were classified into 8 categories which concerned cellular component and biological process, Kyoto Encyclopedia of Genes and Genomes (KEGG) searching showed 4 genes' metabolism pathway. CONCLUSION: Systematic aerobic exercise upregulates expression of enzyme genes concerning tricarboxylic acid cycle, and downregulates expression of genes conceming muscle protein synthesis and sphingolipid. It is suggested that aerobic exercise is good to protect human nerves' integrity, exerts positive action on anti-aging and accelerate the aerobic metabolism of lipid materials in vivo.
3.Evaluation of Microscopic Observation Drug Susceptibility Assay in Detection of Mycobacterium tuberculosis
Youhui FU ; Ruijuan ZHENG ; Jie WANG ; Wenguo JIN ; Zhenling CUI ; Zhongyi HU
Chinese Journal of Nosocomiology 2009;0(23):-
OBJECTIVE To set up and evaluate the microscopic observation drug susceptibility assay(MODS)technology and use it to detect rapidly Mycobacterium tuberculosis(MTB).METHODS The 24-hole cell culture plate method of liquid culture were used to set up MODS technology.The MODS technology was used to detect MTB and non-M.tuberculosis(NMT)isolates comparing with Lowenstein-Jensen(L-J) method.RESULTS When bacterial concentration was 3?103 CFU/ml,the time of reading test-result was the seventh day by MODS;four kinds of NMT(M.phlei,M.kansasii,M.chelonaeand M.marinum) in the liquid medium in the observation was similar to the growth of cording,it was difficult to distinct from the cording of MTB in the liquid medium;When using the 4-Nitro-benzoic acid 800 ?g/ml,thiophene-2-carboxylic acid hydrazine 2.5 ?g/ml for testing conditions,the correct diagnosis can be improved;the test results of clinical isolates by MODS were highly concordance rate with the results of L-J.If the results of L-J was the golden standard,the sensitivity,specificity,positive and negative predictive value as well as accuracy by MODS was 95.7%,100%,100%,99.9% and 97% respectively.CONCLUSIONS The results of MODS in detection of MTB are highly concordance wih the results of L-J method;MODS assay can be used for rapid detection of tuberculosis,with a rapid,simple,inexpensive,and other advantages.
4.Evaluation microscopic observation drug susceptibility assay in detecting mycobacterium tuberculosis in sputum
Youhui FU ; Shu ZHANG ; Haitao WANG ; Feng CAI ; Yanhong FAN ; Zhongyi HU
International Journal of Laboratory Medicine 2016;37(6):728-730
Objective To study the effect of microscopic observation drug susceptibility assay (MODS)for testing Mycobacteri-um tuberculosis in sputum ,and to evaluate its clinical value .Methods A total of 150 sputum samples were collected from patients with pulmonary tuberculosis ,and Mycobacterium tuberculosis were detected by Lowenstein-Jensen (L-J) method and MODS ,and compared the results of the two methods .Results The concordance rates of the test results of sputum sample between MODS and L-J was 77 .8% .The sensitivity and specificity of MODS were 93 .3% and 70 .0% ,positive predictive value (PPV ) was 60 .8% , negative predictive value (NPV) was 95 .4% ,as well as accuracy by MODS was 77 .8% .Conclusion MODS assay could be used as a rapid tuberculosis detection method ,with rapid ,sensitive ,simple ,and other advantages .
5.Biological characteristics of Ebola virus:research advances
Yi ZHANG ; Yinglong QU ; Zhendong GUO ; Siyan ZHAO ; Yingying FU ; Hongyan ZHAO ; Zhongyi WANG ; Zongzheng ZHAO ; Linna LIU ; Jun QIAN
Military Medical Sciences 2015;(5):386-389
Ebola virus disease , which can cause ebola hemorrhagic fever , is a potent zoonotic infectious virus disease . In 2014 , Ebola virus spread across West Africa and it has become a new major threat to global public health .This article summarizes the structural features of Ebola virus , transmission characteristics , interactions ,animal models .
6.Advances in biological spectroscopy detection of pathogenic microorganisms
Zhendong GUO ; Siyan ZHAO ; Yi ZHANG ; Yingying FU ; Hongyan ZHAO ; Yinglong QU ; Zhongyi WANG ; Zongzheng ZHAO ; Jun QIAN ; Linna LIU
Military Medical Sciences 2015;(4):311-315
Detection of pathogenic microorganisms has been a hot research field of microbiology.Conventional detection methods,such as isolation and culture, PCR technology, ELISA and genomic sequencing,are all time-consuming and com-plex.Because of the advantages of quick-testing, accuracy, safety and efficiency, spectroscopy has become a new non-inva-sive testing technology and has witnessed rapid development in pathogen detection and disease diagnosis.This article intro-duces three types of common spectroscopy technologies ( laser excitation fluorescence spectroscopy, infrared spectroscopy and Raman spectroscopy) , and also explains how they work in the detection of pathogenic microorganisms.
7.Quality management at 2 0 hospitals in Shanghai and recommendations
Dan TIAN ; Liebin ZHAO ; Zhongyi JIANG ; Hansong WANG ; Ying ZHANG ; Xiaoting FU ; Weijun GU ; Mengqiao HE ; Liping MA
Chinese Journal of Hospital Administration 2015;(8):611-614
Objective To learn the present hospital management conditions in Shanghai for upgrading quality of care.Methods The stratified sampling method was used for a questionnaire survey of the quality of care at 20 hospitals in Shanghai (10 tertiary hospitals and 10 secondary hospitals).The survey covered such six dimensions as organizational framework,management functions,management tools,professional training,regulations and norms,and supervision over operations.Results Medical quality management system is established at such hospitals and all of them have established medical quality management committees.Rooms of improvement remain in such details as departmental quality management,regulations standardization,IT support,and management tools popularity.Conclusion Management regulations and standards of medical quality management should be improved and implemented from both external and internal aspects,to improve medical quality and patient safety.
8.Research development of human infection with H5N6 avian influenza virus
Lina LIU ; Zongzheng ZHAO ; Zhendong GUO ; Yi ZHANG ; Yingying FU ; Zhongyi WANG ; Hongqi SUN ; Jiaming LI ; Tianrui ZHANG ; Chunmao ZHANG ; Linna LIU ; Jun QIAN
Military Medical Sciences 2017;41(1):69-72
Avian influenza virus is a new recombinant virus , which can cause severe respiratory symptoms ,such as short course,acute disease,and a high mortality rate.The purpose of this paper was to summarize the current status of this virus in terms of its epidemiology ,genetic evolution and virulence .By introducing the advancement in the research of this subtype virus, we hope to provide data and evidence ,for effective surveillance and prevention of this virus .
9.A Feasibility study of hepate ctomy with high-frequency electric welding technique
Fanchun ZENG ; Quanfu CAO ; Fengwen FU ; Xiaoyan ZHANG ; Zhongyi SUN
Chongqing Medicine 2018;47(17):2327-2328,2332
Objective To explore the safety and reliability of soft tissue high-frequency welding technique for partial hepatectomy in animals and provide evidence for its clinical application.Methods With high-frequency welding technique,partial liver tissues were removed from 10 SD rats.Intraoperative blood loss was recorded.Liver function was detected and wound healing was observed by dissecting the rats one week after operation.Results There was no hemorrhage during the liver resection.The liver function returned to normal after one week and the liver incision recovered well.Conclusion The use of soft tissue high-frequency welding technique for partial partial hepatectomy is basically free of bleeding and is safe and reliable.
10.Surgical treatment strategy of gallbladder polyps based on high risk factors of gallbladder cancer
Wencai LYU ; Li JIA ; Shuang WEN ; Zhongyi FENG ; Yunxia LAN ; Weili FU
Chinese Journal of Hepatobiliary Surgery 2017;23(12):861-864
In order to prevent the canceration of gallbladder polyps or avoid the misdiagnosis of gallbladder cancer,the mainstream practice is cholecystectomy for polyps larger than 1 cm,while gallbladder polyps larger than 0.5 cm as an indication of gallbladder preserving surgery.The reasons for this result is that we put the diameter of more than 1 cm of gallbladder polyps as high risk factors for prediction of gallbladder carcinoma,and we ignored the characteristics of gallbladder polyps that most of them are not gallbladder cancer or are not cancerous even larger than 1 cm.We analysed the high risk factors for gallbladder cancer on the basis of variety of clinical data,and we believe that the most valuable indicator of gallbladder cancer are the growth time of gallbladder polyps,the age of the patients,the combination of gallstones,the single polyps,and the sex.Polyps diameter greater than 1 cm are not a high risk factor for gallbladder cancer.We can use these five high risk factors to manage gallbladder polyps and reduce unnecessary cholecystectomy and gallbladder preserving surgery.