1.Recent advances in hippocampal sparing whole brain radiotherapy
Zhongxuan GUI ; Tingting HAN ; Fan WANG
Chinese Journal of Radiation Oncology 2023;32(1):55-59
Whole brain radiotherapy (WBRT) is the standard radiotherapy regimen of preventive radiation for patients diagnosed with brain metastases and non-small cell lung cancer, which can improve intracranial control and prolong overall survival. However, neurocognitive functions (NCF) decline due to impaired hippocampal might occur thereafter. Recent studies have shown that hippocampal sparing WBRT (HS-WBRT) is capable of protecting neurocognitive function and improving quality of life (QOL). In this review, the authors described the methods and significance of hippocampal sparing, summarized the research progress on clinical trials related to HS-WBRT in combination with the development of radiotherapy technology and experimental drugs, and discussed the existing controversies and problems, aiming to provide reference for clinical work.
2.Cinobufagin regulates HIF-1 α/VEGF pathway to reverse drug resistance in colorectal cancer cells HCT15 /5-FU in vitro
Qinying Zhao ; Yue Wu ; Zhongxuan Gui ; Quan Zhang ; Yingquan Ye ; Gaoxiang Wang ; Mei Zhang
Acta Universitatis Medicinalis Anhui 2023;58(6):902-907
Objective:
To investigate the reversal effect of cinobufagin ( CINO) combined with 5-fluorouracil (5- FU) on human colorectal cancer ( CRC) drug-resistant cell line HCT15 /5-FU,and to clarify the regulatory role of hypoxia-inducible factor-1α (HIF-1 α) / vascular endothelial growth factor (VEGF) pathway in reversing chemoresistance of colorectal cancer.
Methods :
MTT assay was used to detect the changes of drug resistance and drug resistance index,flow cytometry was used to evaluate the apoptosis of cells ,scratch test and Transwell assay were used to detect the changes of cell migration and invasion ability.Western blot was used to detect the expressions of epithelial-mesenchymal transition (EMT) related proteins and HIF-1 α/ VEGF pathway-related proteins.
Results:
Compared with HCT15 cells,the resistance index of HCT15 /5-FU was about 8. 720. CINO combined with 5-FU could significantly enhance the drug sensitivity of HCT15 /5-FU cells,reduce drug resistance index,up-regulate the level of apoptosis,and inhibit cell migration and invasion in a dose-dependent manner.Western blot results showed that CINO combined with 5-FU could inhibit the activity of EMT and HIF-1 α/ VEGF pathway.
Conclusion
CINO can reverse 5-FU resistance of colorectal cancer in vitro,and its mechanism may be related to the regulation of the HIF-1 α/ VEGF pathway and the inhibition of EMT and angiogenesis.