Objectives:To investigate the effects of nutritional support by the way of peripheral vein(PV) in the patients of severe acute pancreatitis(SAP). Methods:The treatment results of nutritional support by the way of PV was observed retrospectively within the latest eight years in the patients of SAP. Results:31 cases were cared,and no complications relating to the use of PV in TPN happened. Conclusions:Nutritional support is an important and effective treatment in SAP patients,and the use of PV is an effective and safety route.

Objective To evaluate the antibacterial activity of Galla chinensis etc.14 kinds of TCM against 112 strains of Staphylococcus aureus in vitro. Method Deterimination of antibacterial activity of Galla chinensis etc.against 112 strains of Staphylococcus aureus was performed by using new agar dilution method. Results The antibacterial efficiency of Galla chinensis, Forsythia suspensa and Acacia catechu (L.) Willd against 112 strains Staphylococcus aureus was good, and the MIC90 was 0.102, 0.244, 1.19 mg/mL respectively. Conclusion Galla chinensis etc. 14 kinds of TCM had strong antibacterial activity agsinst 112 srains of Staphylococcus aureus.

Objective To observe antibacterial efficiency of Prunus mume Sieb et Zucc against clinical isolates. Method Antibacterial activity of Prunus mume Sieb et Zucc against 308 strains of clinical isolates were determined by agar dilution method. Results MIC50 of Prunus mume Sieb et Zucc against staphylococcus aureus (112 strains), S.epidermidis (112 strains), and Enterococci (28 strains) were 0.72, 1.44, 0.72 mg/mL, and MIC90 were 1.44, 1.44, 0.72 mg/mL respectively. The MIC90 of Prunus mume Sieb et Zucc to Klebsiella pneumoniae and E. coli were 2.88, 1.44 mg/mL respectively. Conclusion Prunus mume Sieb et Zucc has good antibacterial activity against Gram positive cocci and some Gram negative bacilli.

AIM: The mechanism of extracting flavoinoids from Ginkgo biloba leaves by microwave prtreatment was studied systematically. METHODS: The dired leaves were used as the crude drug, the effect of the operation parameters during the extraction on the extraction result was analyzed with monofactor experiment, and spectrophotometer was used to observe the structure of the cells. RESULTS: After the microwave pretreatment, the structure of the leaves cell was destroyed and the effective composite was desorbed completely. The following extraction rate was 4 times higher that of traditional extraction, the yield and the purity were better than the ethanol extraction. CONCLUSION: The short extraction time, higher yield of effective composite, higher usage rate of the microwave, the method is worthy to be used. W

Objective To identify the genotype of clinical stenotrophomonas maltophilia(SMA) isolates and investigate the characteristics of SMA in nosocomial infections.Methods Totally 165 strains of SMA were clinically isolated during the period of 2004 to 2007.Disc diffusion test(K-B method) was used for antibiotic susceptibility.qacE△1 gene was detected by polymerase chain reation(PCR).The gene homology in the SMA strains was analyzed by pulsed field gel electrophoresis(PFGE).Results Among the tested SMA strains 87.9% sourced from low respiratory tract infection.The antibiotics with more than 80% of sensitive rate against SMA were minocycline,trimethoprim-sulfamethoxazole and levofloxacin.The positive rate of qacE△1 gene was 13.3% in 60 tested strains.The analysis of gene homology for the 11 clinical strains showed that two genotypes from identical clone were found in both respiratory ICU and emergency ICU respectively.Conclusions SMA was an important pathogenic bacterium in nosocomial infections.The treatment for SMA infection is very difficult since its multi-drug resistance.More attention for effective sterilization and isolation of patients must be paid to prevent the transmission of SMA from same clone.