Cardiac muscles of mouse embryos, newborn and suckling mice were used for histological and histochemical studies. This paper presents the observations on the morphogenesis and ultrastructure of cardiac myocytes under electron microscope and on their reactions of nucleic acids (DNA and RNA), glycogen, lipids, succinodehydrogenase (SDH), alkaline and acid phosphatases (AKP and AcP), glucose-6-phosphatase (G-6-Pase) and adenosine triphosphatase (ATPase) under light microscope.The heart of mouse embryo before 12-day, contained numerous polygonal or star-shaped myoblasts which had not yet acquired myofibrils but a few myofilaments. As the myoblast developed, the number of myofilaments increased in number and formed myofibrils, then the cells became myocytes. By the end of embryonic period, all the special elements of myocyte were basically constituted. The myocytes of embryos were rich in RNA granules, and their DNA was deeply stained. Flourishing mitosis appeared only in the early embryonic phase. RNA of adult myocytes was much less than that of embryos. From the early phase of embryos myocytes were full of glycogen but short of lipid droplets. From the day it was born, glycogen decreased apparently but lipid droplets increased rapidly.The reaction of SDH steadily increased in intensity from its early phase to late one. After birth it became more intensive. G-6-pase first appeared in the myocytes of 14 day's embryos. In the fetal period it showed moderate positive reaction, but in the myocytes of suckling muose it appeared negative. The enzyme showed positive reaction again at the age of 2 weeks. The ATPase reaction was found to be weak in the fetal specimens, only appeared in the endothelium of the capillaries. After birth it gradually became intensive and from the 2nd week positive reaction was obvious in adult, it was very vigorous.The above observations showed that the embryonic development and differentiation were gradually completed. Histological and histochemical features of each developmental period showed their individualities, which confirmed the evidence that the cardiac muscle developed not only successively but also by stages, and approached adult's level at 2nd week end after birth.

Objective To investigate whether the serum adiponectin is related to the atherosclerosis in type 2 diabetes.Methods Thirty three normal control subjects(NC group),66 type 2 diabetes subjects without atherosclerosis(NAS group),and 66 type 2 diabetes subjects with atherosclerosis(AS group) were recruited.The intima media thickness(IMT) of dorsalis pedis artery were measured using B-mode Doppler ultrasonography.Serum adiponectin and other gluco-lipid metabolic indices were determined.Results 1)Compared with NC group and NAS group,the IMT value in AS patients were significantly increased(P

Objective To investigate the relationship between benign prostatic hyperplasia (BPH) and urine albumin excretion.Methods A total of 258 male inpatients aged 60 to 70 years were enrolled in this study.According to prostate volume (PV) measured by transrectal ultrasonography,patients were divided into BPH group(PV≥20 ml) and NBPH(non benign prostatic hyperplasia) group(PV＜20 ml).Urine albumin and urine creatinine concentrations were measured and urine albumin/creatinine ratio(UACR) was calculated.Fasting blood glucose(FBG) and fasting insulin(FINS) also were measured,insulin resistance index(HOMA-IR) was calculated based on the formula HOMA-IR=ln(FBG × FINS/22.5).The correlation between PV and other indicators were analyzed by Pearson correlation analysis.The risk factors for BPH were analyzed by logistic regression analysis.Results UACR was significantly higher in BPH group than in NBPH group[(163.6± 69.8) mg/g vs.(73.3 ± 55.6) mg/g,t =4.81,P＜ 0.01],and hypertension duration was longer in BPH group than in NBPH group [(9.2±6.6)years vs.(6.0±3.6)years,t=2.27,P＜0.05].The prevalence rate of BPH and PV were increased along with increasing of urine albumin excretion(x2=4.58,F=4.85,respectively,P＜0.05 or 0.01).Prostate volume was positively correlated with UACR,HOMA-IR,hypertension duration,body mass index(r=0.248,0.229,0.183,0.157,respectively,all P ＜ 0.05).Logistic regression analysis indicated that UACR,HOMA-IR and hypertension duration were the risk factors for BPH(OR=16.348,1.040,0.166,respectively,all P ＜0.05).Conclusions BPH is correlated with UACR,which indicates that there is a close relationship between atherosclerosis and BPH.Insulin resistance probably plays an important role in the progression of arteriosclerosis and BPH.

OBJECTIVE To investigate the prevalence of extended-spectrum ?-lactamases (ESBLs) and antibiotic resistance in Klebsiella pneumoniae isolates.METHODS ESBLs-producers were detected by CLSI phenotypic confirmatory test and susceptibilities were tested by agar dilution method.RESULTS 41.1% Of isolates were ESBLs producers in those isolates. ESBLs producers were highly resistant to piperacillin,cefazolin,cefuroxime,fluoroquinolones,chloramphenicol,SMZ-TMP and amikacin. The resistant rate to piperacillin-tazobactam,and ceftazidime was 26.3% and 34.2%. None was resistant to imipenem and meropenem in ESBLs producers. Except for carbapenems,the resistant rate to other antimicrobial agents was significantly higher in ESBLs producers than non ESBLs producers.CONCLUSIONS With resistance to most of antimicrobial agents,ESBLs-producers are highly prevalent in K. pneumoniae isolates,more attention should be paid to monitor these strains.

Objective To summarize X-ray,CT finding of mediatinal emphysema in the neonate.Methods The X-ray,CT finding of 16 cases with mediatinal emphysema in the neonate were anolysed retrospectively.Results X-ray finding of 16 cases were:around diaphram with a low density band 13 cases,with spinnaker sign 8 cases,with continous diaphram sign 3 cases,there were only little emphysema in anterior-mediatinal 3 cases,accompany with pneumothorax 5 cases.CT imaging of 3 cases was a low density image in diaphram that was CT value was negative(-500～-900Hu).Conclusion Radiography of chest is the first method about diognosis mediatinal emphysema in the neonate,profile chest is more profit to diagnose little emphysema in anterior-mediatinal,CT can diagnose further clear.

Objective To evaluate the diagnostic and therapeutic status of elderly benign prostatic hyperplasia (BPH).Methods Totally 188 male elderly in outpatient department from January 2010 to March 2012 were enrolled in this study.Medical history was inquired in detail,and prostatic volumes (PV)were measured by abdominal ultrasound,PV≥ 20ml was diagnosed as BPH.Lower urinary tract symptoms (LUTS) was evaluated by international prostate symptom score (IPSS),prostate specific antigen (PSA) was measured.Age≥62 years,PSA≥1.6 μg/L and PV≥31 ml were considered as higher risk of clinical progression of BPH.We investigated the rate of prevalence,awareness and treatment of BPH.PV,IPSS and PSA were compared among different age groups by ANOVA.Logistic regression analysis was made to show the risk factors of BPH.Results Among 188 male elderly,the prevalence rate of BPH was 48.4％,and the total rate of awareness was 41.5％.The rate of awareness resulting from seeing doctor due to LUTS was 10.6％.PV,IPSS and PSA were increased with ageing.Risk factors contributing to BPH were age,hypertension and diabetes.The treatment rate with drugs of 5a-reductase inhibitor was 47.3 ％,however 10 subjects had not been given drugs treatment among 46 patients with higher risk of clinical progression of BPH.Conclusions The prevalence rate of BPH is increased with ageing,the proportion of seeing a doctor with passion is lower because the male elderly have not paid enough attention to LUTS.And doctors have not given drugs intervention to patients with clinical progression BPH in time.We should launch propaganda and education among the elderly patients with hypertension and diabetes and take comprehensive treatment.

To study effects of clonidine on release of substance P from rat spinal cord for analysing the spinal sites of its pain relief, Method: Twenty rats were randomly divided into two groups: clonidine group and control group, clonidine 40?g or normal saline was injected intraperitoneally. Immunohistochemica] PAP technique was used to measure substance P in slices of rat spinal cord. Result: The optical density(OD) of immunoreaction of substance P in medial-lateral part of spinal dorsal horn and spinal Lissauer's tract was increased significantly in clonidine group com pared with that of control group (P

OBJECTIVE To analyze the drug resistance of 141 clinical strains of Acinetobacter baumannii to 15 kinds of antimicrobial agents for the rational application of antimicrobial agents.METHODS All 141 strains of A.baumannii isolates were tested for minimal inhibitory concentration(MIC) to 15 kinds of antimicrobial agents included meropenem,imipenem,piperacillin,and so on using agar dilution method.RESULTS The lowest resistance rate of A.baumannii to the antimicrobial agents was to imipenem(22.7%),then was to meropenem and cefoperazone/sulbactam.The highest resistance rate was tetracycline(81.5%).The resistance rate to other antimicrobial agents was to about or more than 50%.CONCLUSIONS Most of A.baumannii isolates are susceptible to meropenem,imipenem,and cefoperazone/sulbactam and resistant to other antimicrobial agents,therefore,we should enhance to this kind of isolates detection.

OBJECTIVE To investigate the resistance and molecular epidemiology of Pseudomonas aeruginosa isolated from ICU. METHODS Susceptibility of P. aeruginosa isolates to 14 antimicrobial agents were tested by microScan WalkAway-40 system,and Molecular typing was analyzed by enterobacterial repetitive intergenic consensus(ERIC-PCR). RESULTS The lowest resistance rate of P. aeruginosa to the antimicrobial agents was to ceftazidime (27.3%),then to cefepime (45.5%). The highest resistance rate was to cefotaxime (100.0%) and the resistance rate to other antimicrobial agents was more than 60.0%. Patterns of ERIC-PCR were same in 5 strains of P. aeruginosa. CONCLUSIONS P. aeruginosa isolated from ICU is resistant to most of antimicrobial agents and clone spread is found in them. We should enhance detection and surveying of these isolates.

OBJECTIVE To investigate the prevalence of meticillin-resistant Staphylococcus haemolyticus(MRSH)and resistance in S.haemolyticus isolates. METHODS MRSH was detected by cefoxitin disc test and susceptibilities were tested by agar dilution method. RESULTS There were 86.4% of MRSH isolates.MRSH was highly resistant to penicillin,cefazolin,cefuroxime,ceftriaxone,tetracycline,ciprofloxacin,and clindamycin.The resistance rate to amikacin,rifampicine and chloramphenicol was 16.9%,11.2% and 28.1%,respectively.All isolates were susceptible to vancomycin and teicoplanin.Except for tetracycline,amikacin,rifampicin,and chloramphenicol,the resistant rate to other antimicrobial agents was significantly higher in MRSH than in MSSH. CONCLUSIONS MRSH is mostly occupied in S.haemolyticus isolates and resistant to most of antimicrobial agents.More attention should be paid to survey and detect these strains.