1.Effects of clonidine on release of substance P from rat spinal cord
Hongbing WANG ; Shiao JIN ; Zhongxin REN
Chinese Journal of Anesthesiology 1994;0(04):-
To study effects of clonidine on release of substance P from rat spinal cord for analysing the spinal sites of its pain relief, Method: Twenty rats were randomly divided into two groups: clonidine group and control group, clonidine 40?g or normal saline was injected intraperitoneally. Immunohistochemica] PAP technique was used to measure substance P in slices of rat spinal cord. Result: The optical density(OD) of immunoreaction of substance P in medial-lateral part of spinal dorsal horn and spinal Lissauer's tract was increased significantly in clonidine group com pared with that of control group (P
2.Isolation of intraperitoneal free cancer cells from colorectal cancer by immunomagnetic beads
Honglei LIU ; Shunmao MA ; Ruifeng REN ; Yitao JIA ; Zhongxin LI
Chinese Journal of Primary Medicine and Pharmacy 2012;19(6):804-805
ObjectiveTo explore the value of immuomagnetic beads(IMB) technique for detection of intraperitoneal free cancer cells from colorectal cancer.MethodsPeritoneal lavage fluid was obtained from 80 patients with colorectal cancer during laparotomy.Peritoneal lavage cytology (PLC) and IMB were used to detect free cancer cells in peritoneal lavage fluid.10 patients with hysteromyoma during laparotomy were enrolled into the control group.ResultsThe positive rate of PLC was 8.8% (7/80),the positive rate of IMB was 28.8% (23/80).The positive case after useing PLC detect,IMB detect also was positive.The detected samples of control group were negative by these two methods.IMB was superior to PLC ( x2 =10.503,P =0.001 ).ConclusionIMB was more sensitive and specific than PLC,which could provide a effective method for finding intraperitoneal free cancer cells.
3.Effects of CDT1 gene over-expression on cell apoptosis and cell cycle in irradiation-induced genomic instable liver cells
Yayi YUAN ; Xuhong DANG ; Yahui ZUO ; Hongyan LIU ; Ruifeng ZHANG ; Zhongxin ZHANG ; Yue REN
Chinese Journal of Radiological Medicine and Protection 2015;35(2):103-106
Objective To investigate the effect of CDT1 gene over-expression on the apoptosis and cell cycle distribution in liver cells with a characteristic of genomic instability induced by radiation(GIR).Methods Lentivirus particles were transferred into liver cells of GIR to up-regulate the expression of CDT1 gene.The apoptosis and the cell cycle were detected by flow cytometry (FCM).The expression changes of p53,ATM,ATR,Bcl-2,and Caspase-3 genes were analyzed by real-time fluorescence quantitative PCR.Results CDT1 gene was efficiently increased by the gene transfection(t =15.56,P < 0.05).In the CDT1 over-expressed cells,while the apoptosis ratio was increased (t =4.19,P < 0.05),the expressions of p53 and Bcl-2 gene were decreased (t =-4.21,-2.06,P < 0.05),but the expression of ATM,ATR and Caspase-3 changed with no significant difference compared with control.Conclusions Over-expression of CDT1 could regulate genomic instability through apoptosis pathway and checkpoint independent of p53.
4. Development and current situation of Chinese prescription medicine in Japan
Chinese Traditional and Herbal Drugs 2016;47(15):2771-2774
Chinese prescription medicine in Japan derives from China's traditional Chinese medicine. Yet during the long histroy of development, the management system of production, quality standard, and registration have been gradually establised in Japan. Research and development pattern and quality control management system for Chinese prescription medicine are formed and are different from those in China. Many of them are different with our current situation, especially in the aspect of quality management. Thus their experience is worth learning and using for reference.
5.Establishment and validation of a dose-response curve for semi-automatic analysis of dicentric chromosomes
Qianqian MENG ; Zhongxin ZHANG ; Yue REN ; Xiaozhen LI ; Zeyu MIAO ; Chao WANG ; Hongyan LIU ; Ruifeng ZHANG
China Occupational Medicine 2024;51(1):70-74
ObjectiveTo establish a dose-effect curve for semi-automatic analysis of dicentric chromosomes(DC) based on an automatic chromosome analysis system. Methods A total of three healthy volunteers were recruited as the study subjects, and their peripheral blood was collected and stimulated by X-ray at doses of 0.00, 0.10, 0.25, 0.50, 0.75, 1.00, 2.00, 3.00, 4.00, and 5.00 Gy, with the absorbed dose rate of 1.0 Gy/min. Images of DC in the mid-stage of cell division were collected using a high-throughput automatic chromosome analysis system. The DCScore software was used to automatically analyze DC aberrations, and a dose-effect curve for semi-automatic analysis of DC was fitted after manual confirmation. The fitted dose-effect curve for semi-automatic analysis of DC was validated for accuracy using three proficiency test samples from the national quality assessment of biological dose. Results The incidence of DC increased with increasing irradiation doses in the range of 0.00-5.00 Gy (P<0.01). The dose-effect curve for the fitted semi-automatic analysis of DC was ŷ =0.000 8 (±0.000 2) +0.009 2(±0.000 9) D+0.014 2(±0.000 4) D2 (R2= 0.999 8). The relative deviation between the estimated dose and the actual dose of the three test samples was about 20.00%, indicating curve applicability for biological dose estimation. Moreover, excluding the time spent on manual analysis, the semi-automatic analysis method increased the analysis efficiency by 26.0 times. Conclusion The semi-automatic analysis dose-effect curve for DC stimulated by X-ray is constructed for biological dose estimation, which can reduce the manual analysis time, and holds great potential for application in nuclear emergency response to large-scale radiation accidents.
6.Video essentials of video-assisted thoracoscopic esophageal replacement with transverse colon
YanJun Dong ; TingKai Ren ; DingQian Zhang ; ZhongXin Yang ; ShuangLin Zhang
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery 2020;27(05):496-497
(正)结肠代食管术(esophageal replacement with colon,ERC)被称作食管外科“皇冠上的明珠”,也是食管重建的最后一种选择。ERC 手术操作复杂,吻合口瘘等并发症发生率较高,一直没有得到广泛开展[1]。传统 ERC 手术常选择经右侧胸腹联合切口,手术创伤较大、术后恢复慢、术后肋间神经损伤等并发症较多,但随着胸腔镜微创技术的日益发展,我们使用胸腔镜微创技术游离胸腔食管并清扫纵隔淋巴结,开腹横结肠代食管行消化道重建,该技术可减少手术创伤、减轻术后疼痛,加速康复。本视频在于展示胸腔镜辅助食管癌根治横结肠代食管术手术入路、操作技巧。
7. Effects of different radiation on chromosome aberration in human lymphoblastoid cells
Ruifeng ZHANG ; Yayi YUAN ; Yue REN ; Zhongxin ZHANG ; Juancong DONG ; Xuhong DANG ; Lihong XING ; Yahui ZUO ; Zhikai DUAN
China Occupational Medicine 2017;44(03):341-344
OBJECTIVE: To compare the effects of ~(56)Fe~(17+),~(12)C~(6+)ion beams and~(60)Co γ rays on chromosome aberration in human lymphoblastoid cells. METHODS: The human lymphoblastoid cells were divided into 0. 1,0. 3,0. 5,0. 7,1. 0,2. 0 Gy irradiated groups and 0. 0 Gy control group. They were separately exposed to ~(56)Fe~(17+)ion beams( linear energy transfer was 400. 0 ke V/μm),~(12)C~(6+)ion beams( linear energy transfer was 26. 0 ke V/μm) and~(60)C γ rays. Chromosome specimens were harvested 48 hours after irradiation. The effects of different radiation on dicentric plus centric ring( “d + r”) aberration rate and chromosome aberration in human lymphoblastoid cells were detected by light microscope with artificial counting. RESULTS: The “d + r”aberration rates induced by 0. 3-2. 0 Gy ~(12)C~(6+)ion beams were significantly higher than those of ~(56)Fe~(17+)ion beams and~(60)Co γ rays at the same dose( P < 0. 017). Chromosome aberration cell rates of 0. 1-2. 0 Gy ~(12)C~(6+)ion beams were significantly higher than those of ~(56)Fe~(17+)ion beams and~(60)C γ rays at the same dose( P < 0. 017). At the dose range of 0. 0-2. 0 Gy,chromosome aberration effects of three kinds of radiations were gradually increased( P < 0. 01). The relative biological effectiveness of ~(56)Fe~(17+)ion beams was lower than that of ~(12)C~(6+)ion beams.CONCLUSION: The chromosome aberration induced by ~(12)C~(6+)ion beams was more serious than that of~(60)Co γ rays and ~(56)Fe~(17+)ion beams.