[Objective]To investigate the clinical effect of minimally invasive technique for patellar fracture with kirschner's wire tensile band through subcutaneously tunnel.[Method]Forty-three cases of closed patella fracture were reduced with minimally invasive, a subcutaneously tunnel was established on the patella surface, blood clot and debris were cleaned through the tunnel. After anatomic reduction, fixed the fracture with Kirschner wire tension band. Function exercise was started early. [Result]All the fractures were approached anatomic reduction and skeletal union. Superficial layer on the patella had no conglutination. The scar and pain in the patella prezone were less than before. The score of Lysholm II was 86.8. Forty-three cases were followed up for an average of 16.3 months. All knee joint could move from 0? to 105?.[Conclusion]Minimally invasive technique for patella fracture with kirschner's tensile band wire could treat patellar fracture reliably and clinical curative effect is excellent.

Objective To investigate the effect of total alkaloids from Rhynchophylline on hypertensive target organs. Methods 40 spontaneous hypertensive rats (SHRs) were divided into 4 groups as follows: normal control group,positive control with Tianma Goutengyin granule at 750 mg?d-1 ,total alkaloids from Rhynchophylline at 2.5 mg?day-1 as low dosage,and at 15.0 mg?d-1 as high dosage.All the rats were fed up for 20 weeks,and baroreflex sensitivity (BRS,in ms?mmHg-1)was determined by revised Smyth’s method.Heart,brain and kidney of the rats were sectioned for histological analysis at the end of 20th week. Results In sixteenth weeks,BRS of positive control,Tianma Goutengyin granule group,total alkaloids at low dosage and high dosage were(0.27±0.05),(0.31±0.06),(0.35±0.08),(0.34±0.08) ms?mmHg-1,respectively.BRS in SHR was not decreased except in the positive control.The treatment groups were superior to the normal control group in structure of heart,but not in the brain and kidney of the rats. Conclusion Total alkaloids from Rhynchophyllinecould protect heart from hypertensive injury.

Objective:To explore the characteristics of color Doppler ultrasound images of breast lymphoma, and to improve the coincidence rate of ultrasound diagnosis.Methods:The color Doppler ultrasound images characteristics of 24 lesions in 21 patients with breast lymphoma in Fujian Cancer Hospital from June 2011 to January 2020 were retrospectively analyzed, and the diagnostic coincidence rate was counted.Results:All the patients were female, 18 cases were unilateral, 3 cases were bilateral. ALL cases were confirmed as primary or secondary breast lymphoma by coarse needle biopsy or surgical pathology. Pathological types included 18 cases (85.7%) of diffuse large B-cell lymphoma, 1 case of anaplastic large cell lymphoma kinase (ALK)-negative anaplastic large cell lymphoma, 1 case of Burkitt lymphoma, 1 case of Burkitt lymphoma or unclassifiable lymphoma with features intermediated between diffuse large B-cell lymphoma and Burkitt lymphoma. The maximum diameter of the lesions was (5.7±2.1) cm (range 1.4-16.0 cm), and all lesions were solid in ultrasound images; 19 lesions (79.2%) were mass-type and 5 lesions (20.8%) were diffuse. According to the characteristics of internal echo, the lesions were divided into hypoechogenicity (2 lesions, 8.3%), hyperechogenicity packed with hypoechogenicity (12 lesions, 50.0%), and hyperechogenicity interweaved with hypoechogenicity (10 lesions, 41.7%). According to Adler semi-quantitative method, the blood flow of the lesions was 3 lesions (12.5%) in grade Ⅰ, 6 lesions (25.0%) in grade Ⅱ, and 15 lesions (62.5%) in grade Ⅲ. The detection rate of lesions by color Doppler ultrasound was 100.0% (24/24), but the diagnostic coincidence rate was only 41.7% (10/24).Conclusions:Most of the breast lymphoma is diffuse large B-cell lymphoma, and the lesions can be effectively screened by color Doppler ultrasound, but the diagnostic coincidence rate is low. Most of the breast lymphoma grows in the form of mass with abundant internal blood flow signals. When the ultrasound image of the breast lesion is hyperechoic packed or interweaved with hypoechoic, the possibility of breast lymphoma should be considered.

Objective To observe the effect of manipulation treatment on headache induced by pure pillow linea nuchae inferior adhesion muscle spasm.Methods 36 headache patients caused by pure pillow linea nuchae inferior adhesion muscle spasm were treated by manipulate the methods of finger press and palm root circle press in base on fixing Ash points according to the symptoms and physical signs.Results After 5 to 10 times by manipulation,of 36 patients,25 cases were recovery,10 cases were excellent,1 case was effective,and the excellence effective rate was 97.22%.Conclusion The manipulation treatment is effect on headache induced by pure pillow linea nuchae inferior adhesion muscle spasm;the curative effect is satisfactory and stable.

ObjectiveTo explore the convenient and effective therapeutic methods for acute lumbar strain on the basis of classification and mechanism of acute lumbar strain.MethodsAcute lumbar strain was classified into post-lumbar joint disturbance type, post-lumbar joint embedded type, muscular strangulated type, muscular strain, sacroiliac joint malposition and mixed type.The repulsion reposition, orthodromic allocation, repulsion ilium and moving leg combioned with high electric frequence were chosen according to the patients' age and somatic condition.ResultsAfter 1-3 times curing, the symptom and appearance of the patients were obviously improved, even more recovery. In these 100 cases,52 patients were cured,39 patients were obviously improved, 9 patients were better than before, the rate of obvious effect was 91%.ConclusionsManipulation combined with high electrical frequence is a convenient, safe and effective method for acute lumbar strain.

Based on the optimized technology on 50L scale, B subtilis 754# was used to manufacture guanosine on pilot scale (12M 3) and plant scale (100M 3) to give the production of 29 4g/L and 21 4g/L successively Through scale down the process of plant scale to pilot scale, based on the theory of dynamic metabolic flux, DO was found to be another key factor that limited the scale up process After conquering the DO limitation, the optimized technology was successfully scaled up to plant scale and the production was further increased by 18% to 25 2 g/L

Objective To study the anti-calcification function properties of bovine jugular conduit with valves stabilized by dye-mediated photooxidation.Methods Sixteen bovine jugular conduit with valves were divided into two groups and treated with dye-mediated photooxidation(groupⅠ) and glutaraldehyde(group Ⅱ).The bovine jugular vein was cut into pieces and implanted subcutaneously in the 16 weanling SD rats.After 90 days,all the rats were sacrificed and the retrieved specimens were undergone histological examination by electron microscope and microscope.The calcium content was determined by flame atomic absorption spectrophotometer.Results The walls and valves of bovine jugular vein treated by dye-mediated photooxidation had less calcification than those of the group Ⅱ.Conclusion The dye-mediated photooxidation can effectively preserve the calcification of bovine jugular conduit with valves compared with the way treated by glutaraldehyde.

BACKGROUND: During recent years, mononucleotide polymorphism of some genes is possibly related to affectability of osteoarthritis (OA). However, previous researches mainly compare the gene expression of synoviocytes between OA and rheumatoid arthritis (RhA); therefore, the correlation of gene expression between synovioblast and fibroblast in other tissues should be further studied as compared with OA.OBJ ECTIVE: To observe the differences of gene expression between OA synovioblast and skin fibroblast.DESIGN: Observational contrast analysis.SETTING: People's Hospital of Shenzhen City.PARTICIPANTS: Synovium tissue was derived from OA patients who received replacement of knee joint in the Department of Orthopaedics, People's Hospital of Shenzhen City. All OA patients met the diagnostic criteria of osteoarthritis established by American College of Rheumatology in 1995. Three patients including 1 male and 2 females aged more than 65 years old and they did not have cardiac and pulmonary disease and diabetes mellitus. Three male normal volunteers who aged 25 to 35 years did not have rheumatic disease, osteoarthritis and dermatosis. All subjects provided a confirmed consent. The main reagents were detailed as follows: RPMI1640 culture medium, fetal bovine serum and TRIZOL agent (Invitrogen Life Technologies Company, USA); pGEM-T pUC (Progema Company, USA);Display PROFILE-BASIC and Display PROFILE Probe kits (Qbiogen Company, USA).METHODS: The experiment was carried out in People's Hospital of Shenzhen City from January to June 2005. Synovium of OA patients were treated with primary culture to obtain synovioblast; meanwhile, skin fibroblast treated with primary culture from normal subjects was regarded as the control group. Restricted enzyme section differential display was used to separate the different-expressed genes of synovioblast and skin fibroblast in OA patients. In addition, blast technique was used to compare the resulted ranks with Genbank ranks.MAIN OUTCOME MEASURES: Differences of gene expression between synovioblast and skin fibroblast in OA patients.RESULTS: Gene expressions of superoxide dismutase (SOD), TFPI2, CXCL2, CXCL6 and transforming growth factor (TGF) were high in synovioblast of OA patients as compared with those in skin fibroblast of normal subjects.CONCLUSION: Gene expressions of SOD, TFPI2, CXCL2, CXCL6 and TGF are high in synovioblast of OA patients as compared with those in skin fibroblast of normal subjects. This suggests that gene may play a certain role in onset of OA.

ObjectiveTo investigate the age-related changes in grip strength of the elderly men and compare with the norms of overseas. Methods112 subjects were divided into six groups: 65～69 years old, 70～74 years old, 75～79 years old, 80～84 years old, 85～89 years old, and 90～95 years old. The grip strength was measured with Jamar dynamometer. ResultsThe grip strength of both sides were significantly moderate negative correlated to age (r=-0.681 and -0.68, P<0.001) and stepwise regression analysis showed the age was important clinical factor to consider in determining grip strength in the elderly men （R2=0.468, F=47.951, P＜0.001). The grip strength was declined significantly during the 75～79 and 85～89 years old. ConclusionGrip strength had declined in the elderly men and these losses were significantly related to age. The highest loss in men was in the age over 75. The norms of grip strength overseas were not applicable to Chinese older adults.

ObjectiveTo determine the test-retest reliability of isometric muscle strength measurement for the lower limb with functional squat system （FSS） in elderly people. Methods20 elderly participants were measured the isometric muscle strength in 5 s of each lower limb with FSS, and retested a week later. The maximal voluntary contraction and the average strength of each lower limb were recorded. ResultsThe intraclass correlation coefficient were 0.77～0.88 (P<0.01). ConclusionIsometric muscle strength measurement of the lower limb with FSS shows acceptable test-retest reliability in elderly.