The early functional changes of the heart after radiation, burn or combined radiation-burn injury were studied with an isolated working heart preparation of rats. The animals were randomized into the control group (C), the burn injury group (B) inflicted with 30% TB-SA full thickness burns from a 5 kw bromine-tungsten lamp, the radiation injury group (R) inflicted with total body irradiation of 6 Gy from a "Co source and the combined radiation-burn injury group (RB) receiving both of the injuries. No treatment was administered after injury. Left ventricular systolic pressure (LVSP). maximum of LV pressure development ( ? dp/ dtmax), heart rate (HR), cardiac output (CO), coronary flow (CF), aortic pressure (A.P) and ratio of dry/wet myocardial weight (Rw) of the perfused isolated heart were determined in the 1st, 3rd, 8th, 16th and 24th hour after injury. It was found that LVSP, ?dp/dtmax, CO and AP were decreased in RB especially in the 8th hour after injury ( P

Objective To increase the recognition and diagnostic rate of multiple myeloma.Methods 43 patients with multiple myeloma confirmed by medula needle biopsy and/or pathology were reviewed.Results Misdiagnosis rate reached 65%.Bone abnormality was not found on X-ray and CT films in 8 cases,the remained 35 cases showed bone destructions in varied degrees and types.Conclusion It's main way for improving diagnostic rate of multiple myeloma that deepen recognition of multiple myeloma.Clinical diagnosis must be combined with imageology,laboratory and pathology.

Objective To explore the suitable delayed time of MSCT mesenteric venography and to probe the value of different post-processing methods.Methods 49 patients with abdominal pain (group A)underwent MSCT mesenteric venography with a delayed time of 15-20 s after the arterial phase,and the CT showed the mesenteric vein(MV)was normal.Meanwhile,other 49 ones with abdominal pain (group B)underwent conventional biphasic CT with a delay-time of 35 s after arterial phase.The CT value and diameter of superior mesenteric vein between two groups were compared,and the grading,scoring and display of mesenteric vein with different post-processingmethods were assessed.Results The diameters of SMV were (10.52±2.19)mm in group A and (9.88±2.60)mm in group B,exhibiting no statistically significant difference (P >0.05);Meanwhile the average attenuations of SMV were (1 96.55±40.59)HU in group A and (1 64.32± 28.35)HU in group B,exhibiting a statistically significant difference (P<0.05).In addition,the display ability and scoring of mesenteric vein and its branches on volume rendering (VR)in group A were better than those in group B (P <0.05).VR was better in display of the vascular space distribution than maximum intensity projection (MIP)and sliding thin slab maximum intensity projection (STS-MIP),the STS-MIP was better in display of tiny blood vessels than VR and MIP,and the curved planner reformation (CPR)was superior in display of vascular lumen and the relationship with adjacent structures.Conclusion The image quality of mesenteric vein with the delayed time of 1 5-20 s after arterial phase is superior to that with delayed time of 35 s,and different advantages of the post-processing methods are showed in display of MV.

Objective To investigate the effects of special anticoagulation method of hybrid coronary revascularization procedure on postoperative bleeding and clinical outcomes.Methods One hundred and forty-one ASA Ⅱ or Ⅲ consecutive patients,aged 35-82 yr,weighing 45-105 kg,undergoing one-stop hybrid coronary revascularization procedure from June 2007 to January 2011 in Fuwai cardiovascular hospital were enrolled and served as Hybrid group.Using propensity score methodology,these patients were matched with another 141 patients who had undergone off-pump coronary artery bypass grafting (OPCAB group) during the same period,selecting from the surgical database.These ASAⅡ or Ⅲ 141 patients,aged 43-80 yr,weighing 47-110 kg,who underwent OPCAB,were served as control subjects.In group Hybrid,the left internal mammary artery was isolated and then 100-120 U/kg heparin was administrated to maintain activated clotting time between 250-300 s,and additional heparin was given if needed.Protamine 1 mg/kg was used to reverse the effect of heparin after anastomosis between left internal mammary artery and left anterior descending branch.Before stent implantation,6000-8000 U heparin and 300 mg clopidogrel were administrated.Activated clotting time was maintained between 200-250 s until the end of operation.Clopidogrel 75 mg/d was taken for at least 12 months after operation.The volume of postoperative chest tube drainage,mechanical ventilation time,length of stay in intensive care unit,postoperative transfusion of allogeneic blood,re-intubation,myocardial damage,acute kidney injury,atelectasis,hydrothorax and thromboembolism were recorded.Results No thromboembolism was observed within the stent during stay in hospital after operation in patients.The volume of chest tube drainage was significantly less,mechanical ventilation time and length of stay in intensive care unit were shorter,the incidence of atelectasis and hydrothorax and transfusion of allogeneic blood requirement were lower in Hybrid group than in OPCAB group (P ＜ 0.05).There was no significant difference in the incidences of re-intubation,myocardio damage,and acute kidney injury between the two groups (P ＞0.05).Conclusion Special anticoagulation method of hybrid coronary revascularization procedure does not increase postoperative bleeding and has a better clinical outcomes during the early period.

Objective To evaluate and follow-up the effection of vertebral artery origin stenting (VAOS) by color Doppler flow imaging(CDFI) and assess the rate of restenosis after stenting. Methods One hundred and thirty-five patients with stenosis of vertebral artery origin segment underwent stent input,135 pieces of stent was input in vertebral artery origin segment with moderate or severe stenosis,in which 70 bare metal stent(BMS) and 65 drug-eluting stent(DES). The diameter of VAOS, peak systolic velocity (PSV),end diastolic velocity (EDV) and resistence index(RI) of segment at origin and cervical vertebral were evaluated before and every 1,3,6, 12 month after VAOS by CDFI. The incidence of restenosis were calculated,and the factors of restenosis were analysed by COX regression. Results The diameter of VAOS was improved from (1.20±0.38) mm to (2.61±0.49) mm after stent procedure ( P = 0. 000), PSV and EDV also decreased from (296.02 ± 113.86)cm/s to ( 113.47 ± 36.35 )cm/s and (90.08 ± 47. 59)cm/s to (32. 21 ± 12. 69)cm/s respectively(P=0.000). The PSV and RI in cervical segment were increased from (46. 88 ± 17.46)cm/s to (67.79 ± 24.31 ) cm/s and 0. 54 ± 0. 10 to 0.62 ± 0.09 respectively( P = 0. 000).Over a median 7 months follow up(range 1 to 12 months) ,the cumulative restenosis rate at 3,6,12 month were 7.9% ,16. 9% and 25.0% respectively. DES was the only one negtive predictor of restenosis(OR=0. 388,95% CI:0.162-0.931, P = 0.034),and the factor of residual stenosis contributed to the occurance of restenosis after stenting(OR = 3.758,95% CI:1.498-9.427, P=0.005). Conclusions CDFI is a sensitive and noninvasive examination to follow-up VAOS and detect in-stent restenosis immediately. VAOS has a high rate of restenosis. DES is effective to prevent in-stent restenosis.

ObjectiveTo investigate the effect of tranexamic acid on inflammatory response in patients undergoing off-pump coronary artery bypass grafting (OPCABG).Methods Sixty ASA Ⅰ -Ⅲ and NYHA Ⅰ -Ⅲ patients of both sexes,aged 45-64 yr,with body mass index 16-22 kg/m2,undergoing elective OPCABG,were randomly divided into 2 groups ( n =30 each):control group (group C) and tranexamic acid group (group T).Anesthesia was induced with iv injection of midazolam,fentanyl and pipecuronium.The patients were tracheal intubated and mechanically ventilated.PETCO2 was maintained at 35-45 mm Hg.Tranexamic acid 1 g was infused intravenously over 30 min after induction followed by continuous infusion at 400 mg/h until the end of operation in group T.While equal volume of normal saline was given in gtoup C.Anesthesia was maintained with inhalation of isoflurane and intermittent (i)v boluses of fentanyl and pipecuroninm.Venous blood samples were taken before induction,at the end of operation and 24 h after operation for determination of Hb,platelet count,prothrombin time,international normalized ratio and plasma D-dimer and IL-6 concentrations.The volume of chest tube drainage was collected and recorded at 6 and 24 h after operation.The requirement for transfusion of allogeneic red blood cells and fresh frozen plasma was recorded.The complications during hospital stay were also recorded.ResultsCompared with group C,the plasma concentrations of D-dimer and IL-6 were significantly decreased at the end of operation and 24 h after operation,and the volume of chest tube drainage and the requirement for transfusion of allogeneic red blood cells and fresh frozen plasma were significantly decreased after operation in group T ( P ＜ 0.05 or 0.01).There was no significant difference in Hb,platelet count,prothrombin time and international normalized ratio between the two groups (P ＞ 0.05).No complications occurred during hospital stay in the two groups.ConclusionTranexamic acid can reduce inflammatory response in patients undergoing OPCABG.

Objective To explore the inhibitory effect of tetramethylpyrazine (TMP) on ultraviolet A-induced senescence as well as matrix metalloproteinase-1 (MMP-1) and-3 (MMP-3) mRNA expressions in human dermal fibroblasts (HDFs).Methods HDFs were isolated from the prepuce by enzymatic digestion, and subjected to primary culture.Cultured HDFs were randomly divided into several groups: control group cultured in high-glucose DMEM medium and receiving no treatment, three TMP groups treated with 20, 50 and 100 mg/L TMP respectively, UVA group receiving UVA radiation alone, UVA + TMP groups pretreated with 20, 50 and 100 mg/L TMP respectively for different durations followed by UVA radiation.UVA radiation was given once daily for 5 consecutive days.The 55th passage HDFs served as the P55 group (senescence control group).Subsequently, CCK-8 assay was performed to evaluate the proliferative activity of HDFs in vitro, optical microscopy to observe the morphologic changes of HDFs after UVA radiation, β-galactosidase staining to estimate the senescence in HDFs, and real-time fluorescence-based quantitative PCR to quantify the mRNA expressions of MMP-1 and MMP-3 in HDFs.Statistical analysis was carried out by one-way analysis of variance (ANOVA) followed by least significant difference (LSD)-t test or Dunnett's T3 test.Results Compared with the control group, the proliferation of HDFs was significantly but transiently inhibited in vitro after the treatment with 100 mg/L TMP for 48 hours (P ＜ 0.05), but showed no significant changes after the treatment with 20 or 50 mg/L TMP for 24, 48 or 72 hours or after the treatment with 100 mg/L TMP for 24 or 72 hours (all P ＜ 0.05).The pretreatments with TMP of 20, 50 and 100 mg/L for 24, 48 and 72 hours all promoted the proliferation of HDFs to a certain degree in the UVA + TMP groups compared with the UVA group, with significant differences in cellular proliferative activity among the UVA group, UVA + TMP groups and control group at 24, 48 and 72 hours (F =17.451,15.231, 23.535, all P ＜ 0.01).Compared with the UVA group, the proliferative activity of HDFs was significantly increased in UVA + 100-mg/L TMP group at 24, 48, 72 hours, UVA + 50-mg/L TMP group at 24 and 72 hours and UVA + 20-mg/L TMP group at 72 hours.After repetitive UVA radiation, HDFs in the UVA group experienced an increase in cell volume, granule acount, and β-galactosidase expression, which was similar to the changes in the P55 group, while the pretreatments with 20, 50 and 100 mg/L TMP for 24 hours suppressed these UVA-induced changes in HDFs.The percentage of β-galactosidase-positive HDFs was 68.417％ ± 1.181％ in the UVA group, 58.167％ ± 5.620％ in the UVA + 20-mg/L TMP group, 45.167％ ± 5.502％ in the UVA + 50-mg/L TMP group, 43.000％ ± 2.000％ in the UVA + 100-mg/L TMP group, 33.667％ ± 5.865％ in the control group, and 76.000％ ± 6.557％ in the P55 group, with significant differences among these groups (F =45.918, P ＜ 0.01).Furthermore, the UVA group significantly differed from the UVA + TMP groups and control group in the percentage of β-galactosidase-positive HDFs and mRNA expressions of MMP-1 and MMP-3 (all P ＜ 0.05).Conclusion TMP can protect HDFs against senescence induced by repetitive UVA radiation, and down-regulate the mRNA expressions of MMP-1 and MMP-3 during senescence.

Objective : To analyze the influencing factors for the molecular network transmission clusters of HIV/AIDS, and to provide scientific evidence for the precise prevention and control of AIDS. Methods: A case-control study was conducted. The HIV/AIDS cases reported from January 2017 to June 2019 in Huzhou with strong clustering of HIV molecular transmission were recruited as the case group, and the residents with same gender, similar age ( ±three years ) and HIV negative in the same community and period were matched ( 1∶4 ) as the control group. Demographic characteristics, AIDS related knowledge and sexual behaviors were collected by a questionnaire survey. The Cox regression model was used to analyze the influencing factors for HIV/AIDS molecular network transmission cluster. Results : There was 100 people in the case group and 400 people in the control group, the differences between them in age, sex, marital status, place of residence and educational level were not statistically significant ( P>0.05 ) . The results of multivariate Cox regression analysis showed that awareness of AIDS related knowledge ( HR=0.145, 95%CI: 0.059-0.352 ) was a protective factor for molecular network transmission clusters of HIV/AIDS; men who have sex wth men ( MSM, HR=9.614, 95%CI: 4.645-19.901) , seeking homosexual partners through the internet (HR=16.321, 95%CI: 7.016-32.968) and having syphilis ( HR=3.314, 95%CI: 1.073-10.232 ) were risk factors for molecular network transmission clusters of HIV/AIDS. Conclusions The awareness of AIDS related knowledge, MSM, seeking homosexual partners through the internet and suffering from syphilis are the influencing factors for molecular network transmission clusters of HIV/AIDS.

Micro-hardness of human teeth was measured by micro hardnessmentry at different depths i.e. enamal, enamelo-dentinal junction and dentin. A simulated dental friction test was conducted of respectively in these three areas, opposing pure titanium ball under a modified fretting machine. The results showed that micro-hardness is 320.40 +/- 22.77 HV of enamal, 232.26 +/- 32.31 HV of enamelo-dentinal junction, and 61.17 +/- 9.42 HV of dentin (P < 0.05). Wear depth is 17.33 +/- 0.58 microns of enamal, 49.44 +/- 16.47 microns of enamelo-dentinal junction and 95.20 +/- 15.07 microns of dentin(P < 0.05). Wear resistance of enamal is much better than that of dentin's. This preliminary investigation should be very useful in clinical applications.
Adult ; Hardness ; Hardness Tests ; methods ; Humans ; Tooth ; anatomy & histology ; physiology

Objective:To construct the molecular transmission network of human immunodeficiency virus type 1 (HIV-1) epidemic strains in northern Zhejiang Province (Jiaxing City and Huzhou City) and to explore the HIV-1 transmission characteristics in this region.Methods:A total of 371 newly diagnosed human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS) patients in Jiaxing City and Huzhou City in 2017 were included as study subjects, and the blood samples were collected and the basic demographic and epidemiological information were obtained. RNA in plasma was extracted, and the pol region gene sequence was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) and nested polymerase chain reaction (PCR) to construct phylogenetic tree for identifying subtypes. The pairwise genetic distances were calculated, and the optimal threshold of genetic distance was selected, and finally the molecular transmission network was constructed. Chi-square test was used for statistical analysis. Results:The pol region gene sequences of 336 samples were successfully obtained, and 11 subtypes were detected, mainly including circulating recombinant form (CRF)07_BC (40.8%, 137/336) and CRF01_AE (31.2%, 105/336). Based on the 1.0% genetic distance threshold, the molecular transmission network of HIV-1 was plotted. A total of 38 transmission clusters (cluster sizes ranging from two to 28) including 119 patients were found, with males predominantly (82.4%, 98/119) and most of the patients aged over 40 (include 40) years old (52.9%, 63/119), mainly infected with CRF07_BC subtype (57.1%, 68/119) and CRF01_AE (24.4%, 29/119). The clustering rate of CRF07_BC (49.6%, 68/137) was significantly higher than that of CRF01_AE (27.6%, 29/105), the difference was statistically significant ( χ2=5.27, P=0.022). Two large clusters C1 (28 cases) and C2 (11 cases) were identified, the majority of which were men who have sex with men (17 cases and seven cases, respectively). High-risk cases generally sought sexual partners in local or nearby cities through mobile phone dating software, of which the infected sequences mostly had high homology with other economic developed regions (Guangdong Province, Beijing City and Hangzhou City, etc.). Conclusions:The HIV-1 subtypes are diverse in Jiaxing City and Huzhou City, mainly CRF07_BC and CRF01_AE. The HIV-1 transmission networks are complex, among which high-risk cases may be the key factor leading to the HIV-1 epidemic in the region. Therefore, it is urgent to deepen the transmission network monitoring and formulate timely precise intervention and prevention strategies.