0 05) Conclusion Most of the CHQ tested (11/15) showed in vitro antiplatelet aggregation activities on human platelet Among them the effect of Fructus Aurantii Immaturus, Pericarpium Citri Reticuatae Viride and Pericarpium Citri Reticulatae were superior or equal to Aspirin

Objective To determine the content of polysaccharide of prepared pinellia tuber. Methods The content was determined by sulfuric acid-anthrone colorimetric method with wavelength at (624?1)nm. Results The content of polysaccharide was 2.533 mg/g in prepared pinellia tuber. The average recovery was 99.54% and RSD was 1.69%. Conclusion The methods was simple, steady and reproducible.

Objective To explore the influence of new Xuefuzhuyu decotion on IgG,C3 and C4 of peripheral blood in atherosclerosis rats.Methods Healthy adult Wister rats were selected and randomly divided into blank group,AS model group,new Xuefuzhuyu decoctioin low dosage group,the new Xuefuzhuyu decoctioin high dosage group,and positive control group,with 13 cases in each group.Vitamin D3 joint high fat feeding method was adopted to establish atherosclerosis rat model.Medicine lavage began on the first day of experiment.Low dosage new Xuefuzhuyu decoctioin,high dosage new Xuefuzhuyu decoctioin,and atovastatin were administrated to the new Xuefuzhuyu decoctioin low dosage group,the new Xuefuzhuyu decoctioin high dosage group,and positive control group respectively,and distilled water 15 ml/kg · d-1 was administrated to both the blank group and the AS model group.The weight of body was detected each week in each group for regulating the administrated dosage.After medicine lavage was performed for successive 8 weeks,blood was obtained from abdominal aorta for detecting IgG,C3,C4 by immune scattering method.Results The value of IgG,C3 and C4 were (8.110±0.720)g/L,(1.591±0.143)g/L,(0.390±0.33)g/L in the AS model group,(8.000±0.840)g/L,(1.564± 0.132) g/L,(0.396±0.36)g/L new Xuefuzhuyu decoctioin low dosage group,(7.250±0.740)g/L,(1.390±0.150)g/L,(0.326±0.153)g/L new Xuefuzhuyu decoctioin high dosage group and (7.240±0.400) g/L,(1.414 ± 0.104) g/L,(0.330±0.164) g/L in the positive control group,all of which werehigher than the blank group (5.810±0.820)g/L,(0.943±0.124)g/L,(0.330±0.164)g/L with statistically significant difference (P＜0.05).Each value of IgG,C3 and C4 in the new Xuefuzhuyu decoctioin high dosage group and the positive control group were lower than the AS model group and the new Xuefuzhuyu decoctioin low dosage group,with a statistically significant difference(P＜0.05).There was no significant difference between new Xuefuzhuyu decoctioin high dosage group and the positive control group (P＞0.05).Conclusion New Xuefuzhuyu decoction can ameliorate IgG,C3 and C4 of peripheral blood in atherosclerosis rats.Its function was similar to atorvastatin.

Objective To summarize the clinical outcome of single posterior fixation and fusion in treatment of cervical spinal fracture combined with ankylosing spondylitis.Methods A total of 26 patients with cervical spinal fracture combined with ankylosing spondylitis admitted for surgical treatment from June 2003 to June 2008 were reviewed to analyze parameters including injury mechanism,injury severity,treatment course,operation records,postoperative follow-up and rehabilitation and evaluate neurologic recovery and fracture healing by using American Spinal Injury Association(ASIA)classification.Results A single posterior surgical procedure was performed in six patients,of whom the spinal cord function were rated at grade A in two patients,at grade B in one,at grade C in two and at grade D in one according to ASIA classification criteria.All patients were treated with posterior reduction and lateral mass fixation plus fusion.Two patients at grade ASIA A died of respiratory failure at 2 and 3 months respectively after operation.The other four patients were followed up for 12-54 months(mean 37 months),which showed solid fusion at average 3.8 months after operation and significant improvement of neural function.One patient with aggravated symptoms of spinal epidural hematoma at grade ASIA B was improved to grade D postoperatively.The patients at grades C and D recovered to normal.Hyponatremia occurred in two patients at grade ASIA A.There was no instrumentation related complications in all patients.Conclusions For cervical spinal fractures combined with ankylosing spondylitis without bone defect at fracture end,lateral mass fixation and autologous bone graft through a single posterior approach can attain stable fracture healing after sound reduction.

Objective:To investigate the relationship between ADAM 33 polymorphisms and susceptibility of asthma of Chinese in Qingdao.Methods: The polymorphism of ADAM33 gene, they were rs2280090, rs487377 and rs2787094, were detected with SnaPshot method.Results: There was no significant difference between asthma group and control group in genotypic frequencies.Conclusion:The study shows that the rs 2280090 , rs487377 and rs2787094 were not associated with susceptibility of asthma in Han nationality in Qingdao .

OBJECTIVETo assess the association of polymorphisms of IL-4 gene (rs2243250, rs2243283) and IL-4R gene (rs1805012, rs1801275, rs1805010) with susceptibility to asthma among ethnic Chinese in Qingdao.

METHODSFor 400 asthma patients and 200 healthy subjects, above polymorphisms were detected with SnaPshot method.

RESULTSFor rs1805012, the frequency of TC genotype in the asthma group was significantly lower than the control group (8.8% vs. 15.5%, χ (2)= 6.498, P= 0.039), and so were the frequencies of TC+ CC genotypes (9.0% vs. 15.5%, χ (2) = 5.522, P= 0.019) and the C allele (4.6% vs. 7.7%, χ (2) = 4.729, P= 0.039). No significant difference was detected between the two groups in the frequency of the remaining four polymorphisms or the haplotypes formed by rs2243250 and rs2243283 (All P> 0.05).

CONCLUSIONThis study has indicated that rs1805012 polymorphism of IL-4R gene is associated with asthma in ethnic Han Chinese from Qingdao region. TC+ CC genotypes have a protective role against asthma compared with TT genotype. However, polymorphisms of IL-4 gene are not associated with susceptibility to asthma.

Alleles ; Asian Continental Ancestry Group ; genetics ; Asthma ; Female ; Genetic Predisposition to Disease ; Genotype ; Humans ; Interleukin-4 ; genetics ; Male ; Middle Aged ; Receptors, Interleukin-4 ; genetics

OBJECTIVE To assess the association of polymorphisms of TNF-alpha gene (rs1799724, rs1800630, rs1799964 and rs769178) and IL-13 gene (rs2158177 and rs1295687) with susceptibility to asthma among ethnic Chinese in Qingdao region. METHODS For 400 asthma patients and 200 healthy subjects, above polymorphisms were detected with a SNaPshot method. RESULTS For rs2158177, the frequency of genotype of GG in the asthma group was significantly lower than the control group (2.8% vs. 5%, OR = 0.31, 95%CI: 0.12-0.82, P = 0.021). No significant difference was detected in the genotypic frequencies for the remaining 5 polymorphisms between the two groups (All P > 0.05). CONCLUSION The study has indicated that rs2158177 polymorphism of the IL-13 gene is associated with asthma in ethnic Han Chinese from Qingdao. No association has been found between polymorphisms of TNF-alpha gene with susceptibility to asthma.
Adolescent ; Adult ; Aged ; Asthma ; genetics ; Female ; Humans ; Interleukin-13 ; genetics ; Male ; Middle Aged ; Polymorphism, Single Nucleotide ; Tumor Necrosis Factor-alpha ; genetics