Objective To investigate the correlation between plasma fibronectin (Fn) level and the hemorrhagic transformation (HT) in patients with acute ischemic stroke.Methods Patients with acute ischemic stroke,symptom onset within 72 hours,no bleeding at baseline MRI (including gradient echo sequences) and without thrombolytic therapy were recruited prospectively.At 7 - 10 days after symptom onset reexamined MRI.The patients were divided into HT and non-HT groups according to whether they had a hemorrhage or not.The plasma Fn levels in both groups were compared.Logistic regression analysis was used to investigate the predictors of HT.Results A total of 78 patients were included,and among them 11 patients (14.10％ ) showed HT.The proportions of atrial fibrillation (36.4％ vs.9.0％,P =0.012) and cardioembolism (36.4％ vs.6.0％,P =0.021) in the HT group were significantly higher than those in the non-HT group.The baseline mean systolic blood pressure (182.09 ±20.73 mm Hg [ 1 mm Hg=0.133 kPa]vs.161.25 ±26.40)mm Hg,P =0.015),mean diastolic blood pressure (98.00 ± 11.26 mm Hg vs.86.60 ± 15.21 rnm Hg,P =0.020),and plasma Fn level (4 835.04 ± 756.30 μg/L vs.3 849.44 ± 1 289.18 μg/L,P =0.016) were all significantly higher than those in the non-HT group.Multivariate logistic regression analysis showed that plasma Fn level (odds ratio [ OR],1.001,95％ confidence interval [ CI] 1.000 - 1.001,P =0.017) and atrial fibrillation (OR,13.408,95％ CI 1.963 -91.592,P =0.008) were the independent predictors of HT in patients with acute ischemic stroke.Conclusions The plasma Fn level in patients with HT increased significantly.The plasma Fn level can be used as an independent predictor of non-thrombolytic HT after the onset of acute ischemic stroke.

Objective To study the characteristics of CT images of myocardial bridge(MB)in patients with atherosclerosis.Methods CTA images of MB in 129 patients with atherosclerosis were studied.Another 109 patients without atherosclerosis,in the diagnosis of MB were used as control.The type,age,thickness of MB,length of mural coronary artery and end systolic diameter of mural coronary artery were compared between the two groups.Results In the study group,78 cases (60.5%)were superficial type,51(39.5%) were deep type.While in the control group,70(64%)cases were superficial type and 39(36%)were deep type.There was no significant difference between the two groups.The age,thickness of MB,length of mural coronary artery and end systolic diameter of mural coronary artery in each group were 57.01±10.17 years old,(3.15±1.66)mm,(20.43±7.38)mm,(1.16±0.25)mm and 48.36±9.11 years old,(1.95±1.77)mm,(21.07±6.69)mm,(2.07±0.81)mm.These parameters had significant differences between the two groups except the length of mural coronary artery (P>0.05).Conclusion The MB of the study group is thicker than the control group,and the mural coronary artery diameter of the former is narrower than that of the latter.

Objective To analyze the CTA features of asymptomatic myocardial bridge.Methods The CTA images of 69 cases with asymptomatic solitary myocardial bridge were studied retrospectively, and CTA images of 60 cases with symptoms as the contrast group.The type, age, thickness of myocardial bridge, mural coronary artery length and diameter changes of each cases of two groups were analyzed.Results In the study group, 51 cases of 69 (74%) were superficial style, while 18 cases were deep type (26%).In the contrast group, the superficial and deep style were 13 (22%) and 47 (78%) respectively.The mean age,thickness of myocardial bridge,mural coronary artery length and the diameter of mural coronary artery were (53.01±11.17) years old,(1.25±1.16) mm,(21.33±7.32) mm,(2.86±0.45) mm and (51.36±9.31) years old,(1.45±1.87) mm,(20.07±6.60) mm and (1.37±0.41) mm.The rate of type and diameter of mural coronary artery had significant differences between two groups (P<0.05), while other data had no significant differences (P>0.05).Conclusion The CTA features of asymptomatic myocardial bridge are mostly superficial type.The diameter of mural coronary artery on the end systolic is a factor to judge the rate on the occurrence of clinical symptom.

Objective To improve the skills and level of TURP and peri-operative period managements to the high risky senile patients with large volume benign prostatic hyperplasia.Methods The clinical data of 318 high risky and senile patients whose ages above 80ys,ASA score ＞ 2 and prostate volume ＞ 60g were analyzed retrospectively.They underwent the treatment of TURP.ResultsTotal 318 patients underwent TURP were safe.The operating time ranged from 40 to 85 minutes,averaged 58.2minutes;the volume of blood transfusion ranged from 200ml to 600ml;No serious complications happened during and after the operation.With follow up of 1 ～ 12 months,the International Prostate Symptom Scores(I-PSS) decreased 14.7 averagely,Quality of Life(QOL) decreased 3.3 averagely,Maximal flow rate( Qmax ) increased 6.4ml/s averagely,and Post-voided Residual(PVR) decreased 85.3ml averagely.Conclusion The actions including sufficient preparations and evaluations pre-operatively,the maintenance to the stability of circulatory system during the operation,guaranteeing the demands of blood exchange in the vital organs such as heart,lungs and brain,are the key points to the success.

Objective: To study the characteristics of coronary CT angiography (CTA) in patients with myocardial bridge (MB) with arrhythmia. Methods: Our study included 2 groups: MB+arrhythmia group,n=31, clinical information as medical record, electrocardiogram (ECG), myocardial enzyme, echocardiography and coronary CTA findings were collected; MB group, n=30, the MB patients were without arrhythmia. Results: In MB+arrhythmia group, all patients were with mere MB, coronary artery disease, valve-structural heart diseases and other systemic diseases were excluded. There were 2/31 patients with ventricular fibrillation, 1 with atrial fibrillation, 5 with supraventricular tachycardia and 23 with ventricular tachycardia; 17/31 patients having deep type MB and 14 having superficial type MB. The myocardial systolic end diameter, diastolic end diameter by retrospective ECG gating and the stenosis at cross section of mural coronary MB by CTA were similar between 2 groups,P>0.05. Conclusion: MB+arrhythmia patients had no specific characteristics in coronary CTA; anatomical CTA feature may partly explain the myocardial ischemic symptom while couldn't clarify arrhythmia occurrence in relevant patients.

ObjectiveTo improve the treatment of urethral stricture and evaluate the applicative effects of wire-guided supporting catheterization in clinical urology. MethodsDuring January 2004 and December 2005, the patients with urethral stricture were dwelled with the catheters using the traditional guideless catheterization (group A). If the dwelling failed,it will be replaced by the wire-guided supporting catheterization using the improved catheters. During January 2006 and December 2009, the patients with urethral stricture were dwelled with the improved catheters using wire-guided supporting catheterization ( group B) straightly. Compared the first-time dwelling success incidence,the incidence of catheter associated urinary tract infection and side-effect events. Then after 1 year, compared the urethral stricture recrudescence and the course of treatment. ResultsThe success incidence of first-time catheterization in group A was 48.98％ (24/49) ,and in group B was 97.94％ (95/97) ,there was significant difference between 2 groups;The incidence of catheter associated urinary tract infection in group A was 12.24％, and 8. 25％ in group B, there was significant difference between 2 groups; As for the course of treatment, group A was 46. 2w,group B was 32.7w;The urethral stricture recrudescence in group A and B were 16.33％ and 9.28％ respectively,and there was significant difference between 2 groups. ConclusionThe wire-guided supporting catheterization, which minimize the injury and simplify the operation of internal urethrotomy,could makes the improved catheter easy to induct and replace, improve the success rate of first time-catheterization and prevent the false tunnel damage and new scar expansion. It could make benefit to reduce of incidence of catheter associated urinary tract infection and urethral stricture recrudescence,but also could shorten the course of treatment significantly.

AIM: To explore the molecular mechanism in the pathogenesis of dilated cardiomyopathy (DCM) by analyzing the expression of T cell signaling molecules in mice with autoimmune DCM. METHODS: Mouse DCM model was induced by immunizing the animals with adenine nucleotide translocase (ANT) synthetic peptides. P56lck in T cells was detected with real-time fluorescent quantitative PCR in both DCM-group and the sham-immunized controls. At the same time, flow cytometry was used for quantity of Th cell intracellular cytokine IFN-? and IL-4, ELISA for examining the level of serum anti-ANT antibody, immune histochemistry for investigating the expression of CD45 in Th cells. RESULTS: The mRNA expression of P56lck ( 1 369.51 ?874.05 vs 47.93?10.21, P

e chain-link flap is the good way in the treatment of large areas of the lower leg soft tissue defects.

The swollen root of Rehmannia glutinosa is used as one kind of important Chinese traditional medicine. The root of R. glutinosa usually swelled in rotational cropping but not in continuous cropping. The rhizosphere microorganisms of R. glutinosa under different farming condition were thought related to that. In this study, the endophytic fungi in the root of R. glutinosa growing in various soil conditions were isolated for the study of the relationship between the microorganisms and the root enlargement of their host plants. The dominant endophytes, Verticillium spp., Fusarium oxysporum, F. redolens and Ceratobasidium spp. were identified by morphological observation and 18S rDNA and ITS sequence analysis. The preliminary investigation showed that the excessive growth of Verticillium and Fusarium genus fungi is unfavorable for the R. glutinosa root swelling, but Ceratobasidium fungi has no effects on the root enlargement.
DNA, Fungal ; genetics ; DNA, Ribosomal ; genetics ; Fungi ; classification ; genetics ; isolation & purification ; Molecular Sequence Data ; Plant Roots ; microbiology ; Rehmannia ; microbiology

ObjectiveTo establish a droplet digital PCR (ddPCR) method for detecting hepatitis B virus (HBV) covalently closed circular DNA (cccDNA). MethodsHBV cccDNA standard substance was constructed, and HBV cccDNA primers and probes were designed based on the structural differences between HBV cccDNA and relaxed circular DNA (rcDNA). HBV plasmid was amplified to obtain HBV cccDNA standard substance, and a ddPCR detection method was established with the standard substance after gradient dilution as the template for HBV cccDNA detection; the limit of detection and repeatability of this method were analyzed. Liver tissue samples were collected from 20 patients who attended Beijing YouAn Hospital, Capital Medical University, from June 2017 to October 2020, all of whom were diagnosed with HBV infection, and DNA of the samples was extracted and digested with plasmid-safe ATP-dependent DNA enzyme to obtain HBV cccDNA template; the ddPCR detection method was evaluated in clinical samples and was compared with the quantitative real-time PCR (qPCR) detection method. The chi-square test was used for comparison of categorical data between the two groups. ResultsThe HBV cccDNA detection method based on ddPCR was established, which accurately detected HBV cccDNA in standard substance after gradient dilution, with a limit of detection of 1 copy/μl, and the coefficients of variation of 1×103, 1×102, and 1×101 copies/μl standard substances were 441%, 3.98%, and 5.09%, respectively. HBV cccDNA was detected in the samples of 20 patients with HBV infection; the ddPCR detection method detected HBV cccDNA in 17 patients, with a positive rate of 85%, while the qPCR detection method detected HBV cccDNA in 11 patients, with a positive rate of 55%, and there was a significant difference between the two methods (χ2=4.286, P=0038). ConclusionThe established ddPCR method for detecting HBV cccDNA has a low limit of detection and good repeatability, which provides an effective tool for further clinical detection.