Objective:To investigate the clinical application of PPH plus external hemorrhoidectomy in the treatment of hem- orrhoids. Methods:Sixty-eight cases of Ⅲ-Ⅳdegree Ring-like mixed hemorrhoids treated by PPH from January 2004 to Jan- uary 2007 were respectively reviewed. Results:A post operation follow-up found no anus incontinence, stenosis of stoma and recurrence. Conclusion:PPH plus external hemorrhoidectomy can widen the indications for PPH and improve patients’satis- faction.

Objective:To compare with curative effect,hospital stay,average cost,recovery time and postoperative complication between local anesthesia and epidural anesthesia stratege for tension-free hernioplasty in middle or old-aged patients.Methods:164 cases were randomly divided into local anesthesia and epidural anesthesia groups.Results:Mean operating time,the time of hospital stay and the rate of postoperative complication in local anesthesia groups were less than that in epidural anesthesia groups.Conclusion:Tension-free hernioplasty under local anesthesia,a more safe,effective and cheap operating method in middle or old aged patients with inguinal hernia,should be widespread.

Objective To compare the clinical effects of laparoscopy and traditional open surgery in the treatment of left colon cancer.Methods 58 patients with left colon cancer were randomly divided into the control group (27 cases) and the treatment group (31 cases).The control group was treated by traditional open surgery,the treatment group was treated by laparoscopic surgery.The operative time,blood loss,postoperative analgesia,postoperative exhaust time,length of hospital stay and number of lymph node dissection of the two groups were observed and compared.The postoperative complications were observed.Results The blood loss,postoperative analgesia,postoperative exhaust time,length of hospital stay of treatment group were less than those of the control group (t =0.648,0.619,0.588,0.497,all P ＜ 0.05) ; while the operative time,the number of lymph nodes dissection of the two groups showed no significant differences (t =0.135,0.209,all P ＞ 0.05).The incidence rate of postoperative complications of the control group was 18.5％,which was significantly higher than 9.7％ of the treatment group (x2 =17.03,P ＜ 0.05).Conclusion Laparoscopic surgery in the treatment of left colon cancer has less blood loss,shorter hospital stay,fewer complications,it has high clinical application value.

The preclinical safety assessment of hepatotoxicity drugs has a low sensitivity and low specificity. Related tests often generate false negative results and unexpected toxicity,which is one of the major reasons for the cessation of development and withdrawal from the market. Proteomics enjoys advantages of rapidness,high sensitivity and high throughout,and therefore can be used in the search for new biomarkers of hepatotoxicity in preclinical studies,leading to the development of safer drugs and a more efficient drug discovery process. In this review,the current preclinical biomark?ers of liver toxicity and development of proteomic technologies in the discovery and validation of bio?markers of drug-induced liver injury are described,in general the application of proteomics to Chinese medicine-induced liver toxicity in particular. Compared with traditional methods,proteomic technologies show promising results for the discovery of novel hepatotoxic markers. Proteomics,in conjugation with other omics techniques,will play a major role in the early stage of hepatotoxicity screening and will prove to be a good bridge in clinics in the future.

OBJECTIVE:To provide references for Chinese pharmaceutical enterprises in establishing developmental strategy for generic drug market.METHODS:This study was bases on the theory of marketing together with literature re?trieval and demonstration analysis.RESULTS&CONCLUSION:This paper has summarized the chief problems lies in the Chinese generic drug market and put forward some feasible strategies for the pharmaceutical enterprises such as to choose va?rieties of specific features based on the characteristics of enterprises;to achieve innovation in the marketing and to focus on the country market so as to enlarge the market share;to establish strategic alliances so as to strive to be the leader of generic drugs through collaboration and competition;to occupy the top market by establishing brands for generic drugs.

Diabetic retinopathy (DR) is one of the signs of complications related to diabetes in eye In clinic,there has not perfect measurement and method to prevent and treat DR.Proteomics as an emerging subject focuses on studying the organizational structure and related functions as a whole level.The application of proteomics research method can fully reveal the protein expression in tissues and cells with comparison of the difference between the normal and diabetes.The differentially expressed proteins can reveal the various factors in the pathological process of DR to address the accurate regulatory mechanism,and can find some new regulatory proteins associated with DR.This approach provides further theory and methodology to study DR,which has its unique advantages in drug-targeted treatment of DRs.

Objective To determine the effect of miR-138 on SD rat renal ischemia-reperfusion injury its mechanism.Methods 60 adult SD rats were randomly divided into Sham group,renal ischemia-reperfusion (RIRI) group,RIRI + vector group,RIRI + miR-138 group,RIRI + miR-138 shRNA group,and then we established renal reperfusion injury model by occlusion bilateral renal pedicle.Creatinine,blood urea nitrogen in serum are tested to compare differences in renal function in each group,and TUNEL staining was taked to detect apoptosis,real time PCR were taked to test the exression of p53 and it's pathway.Results When compared with the sham group,expression of kidney function BUN/Crin serum,percentage of apoptoiscells of reperfusion injury group wrer all increased,and the differences were statistically significant (P ＜ 0.05).Upregulate miR-138 increased these indexes,and the difference was statistically significant (P ＜0.05),and down regulate miR-138 would protect the injure of renal ischemia-reperfusion.Further studies showed that miR-138 may play the role by inhibiting the p53 signaling pathway.Conclusion MiR-138 has a significant aggravating effect on renal ischemia-reperfusion injury,and the mechanism may be related to p53 signaling pathway.

Objective To investigate the timing of cholecystectomy in patients with mild biliary acute pancreatitis.Methods 80 patients with mild acute biliary pancreatitis were prospectively collected.According to the principle of random digital table, the patients were randomly divided into the study group and control group,40 cases in each group.The study group underwent early cholecystectomy, while the control group received delayed cholecystectomy.Main indicators included biliary tract complications, perioperative complications, conversion to open surgery, operation time and total hospital stay were observed.Results Compared with the control group, the total hospitalization time of the study group was significantly shorter[(9.39±2.48)d vs.(11.48±3.28)d,t=3.582,P=0.000].There were no statistically significant differences in the rate of conversion to open surgery, operation time, perioperative complications and mortality between the two groups (P>0.05).The incidence rates of acute cholecystitis, biliary colic and total biliary complications of the study group were 0.00%,0.00% and 0.00%, which were significantly lower than those in the control group (15.00%,22.50% and 47.50%) (x2=4.505,8.013 and 22.364,P=0.034,0.005 and 0.000).Conclusion Early laparoscopic cholecystectomy is helpful to reduce the incidence of adverse events in the biliary system.

Effective strategies to sterilize and disinfect the surface of sickbed should be adopted before the sickbed is moved into or out of an operation room.Ozone produced by Nano technology is used as disinfector.The experiments shows that the killing logarithm value to kill nature bacteria is between 0.54 and0.85 in short time.The effect of sterilization and disinfection for the surface of sickbed is satisfying without secondary pollution,so the new method can be used to sterilize and disinfect sickbed.

General Policies of Nomenclature for Biological Products in China were studied,aimed at establishing and perfecting the system of Chinese approved biologic name.By means of analysis of the current situation and existing problems of nomenclature for biological products and the development trend of new biotherapeutics.It is sufficiently studied on the existing leading international drug generic naming system,and the specific suggestions on revision of the General Policies of Nomenclature for Biological Products in China based on the naming principles of WHO INN and Chinese language recognition were put forward.