Objective To compare the clinical effects of laparoscopy and traditional open surgery in the treatment of left colon cancer.Methods 58 patients with left colon cancer were randomly divided into the control group (27 cases) and the treatment group (31 cases).The control group was treated by traditional open surgery,the treatment group was treated by laparoscopic surgery.The operative time,blood loss,postoperative analgesia,postoperative exhaust time,length of hospital stay and number of lymph node dissection of the two groups were observed and compared.The postoperative complications were observed.Results The blood loss,postoperative analgesia,postoperative exhaust time,length of hospital stay of treatment group were less than those of the control group (t =0.648,0.619,0.588,0.497,all P ＜ 0.05) ; while the operative time,the number of lymph nodes dissection of the two groups showed no significant differences (t =0.135,0.209,all P ＞ 0.05).The incidence rate of postoperative complications of the control group was 18.5％,which was significantly higher than 9.7％ of the treatment group (x2 =17.03,P ＜ 0.05).Conclusion Laparoscopic surgery in the treatment of left colon cancer has less blood loss,shorter hospital stay,fewer complications,it has high clinical application value.

Objective:To investigate the clinical application of PPH plus external hemorrhoidectomy in the treatment of hem- orrhoids. Methods:Sixty-eight cases of Ⅲ-Ⅳdegree Ring-like mixed hemorrhoids treated by PPH from January 2004 to Jan- uary 2007 were respectively reviewed. Results:A post operation follow-up found no anus incontinence, stenosis of stoma and recurrence. Conclusion:PPH plus external hemorrhoidectomy can widen the indications for PPH and improve patients’satis- faction.

Objective:To compare with curative effect,hospital stay,average cost,recovery time and postoperative complication between local anesthesia and epidural anesthesia stratege for tension-free hernioplasty in middle or old-aged patients.Methods:164 cases were randomly divided into local anesthesia and epidural anesthesia groups.Results:Mean operating time,the time of hospital stay and the rate of postoperative complication in local anesthesia groups were less than that in epidural anesthesia groups.Conclusion:Tension-free hernioplasty under local anesthesia,a more safe,effective and cheap operating method in middle or old aged patients with inguinal hernia,should be widespread.

ObjectiveTo observe the effect of probucol combined with atorvastatin in treatment of stenosis carotid atherosclerosis.MethodsEighty-six patients with stenosis carotid atherosclerosis were separated into two groups with 43 cases each by random digits table.The patients were treated with probucol combined with atorvastatin in observation group and atorvastatin only in control group.The changes of arteriosclerotic plaque and blood fat pre- and poet-treatment were observed.Results There was no significant difference in the levels of lumen area (LN) and intima-media thickness (IMT) pre-treatment between two groups (P＞ 0.05).Compared with pre-treatment,the levels of LN and IMT post-treatment were significantly decreased in observation group(P ＜ 0.05 ),while no significant difference in control group (P ＞0.05).There was significant difference in the levels of LN and IMT post-treatment between two groups [(0.59 ±0.19) cm2 vs.(0.72 ±0.28) cm2,(2.76 ±0.41 ) mm vs.(3.27 ±0.57) mm](P＜ 0.05).There was no significant difference in the levels of total cholesterol (TC),triacylglycerol (TG),low density lipoprotein cholesterol (LDL-C),high density lipoprotein cholesterol (HDL-C) pre-treatment between two groups (P ＞0.05).Compared with pre-treatment,the levels of TC,TG,LDL-C post-treatment were decreased,HDL-C was increased,there were significant differences (P ＜ 0.05 ).Post-treatment,the levels of TC,TG,LDL-C in observation group were lower and HDL-C was higher than those in control group (P ＜ 0.05).Conclusion Probucol combined with atorvastatin in treatment of stenosis carotid atherosclerosis can improve arteriosclerotic plaque and low blood fat,which can be applied in clinic.

Effective strategies to sterilize and disinfect the surface of sickbed should be adopted before the sickbed is moved into or out of an operation room.Ozone produced by Nano technology is used as disinfector.The experiments shows that the killing logarithm value to kill nature bacteria is between 0.54 and0.85 in short time.The effect of sterilization and disinfection for the surface of sickbed is satisfying without secondary pollution,so the new method can be used to sterilize and disinfect sickbed.

General Policies of Nomenclature for Biological Products in China were studied,aimed at establishing and perfecting the system of Chinese approved biologic name.By means of analysis of the current situation and existing problems of nomenclature for biological products and the development trend of new biotherapeutics.It is sufficiently studied on the existing leading international drug generic naming system,and the specific suggestions on revision of the General Policies of Nomenclature for Biological Products in China based on the naming principles of WHO INN and Chinese language recognition were put forward.

Objective To observe the clinical efficacy of atorvastatin combined with neuroendocrine antagonist in the treatment of chronic heart failure.Methods 70 patients of Wenzhou Medical College Theorem Clinical College of Cardiology Department from March 2014 to March 2015 were randomly divided into observation group and control group of 35 cases in each group.The control group were given the neuroendocrine antagonist, observation group were given atorvastatin combined with neuroendocrine antagonist treatment. The changes of related indicators were recorded. Results The aminoterminal pro-brain natriuretic peptide (NT-ProBNP) and ejection fraction in observation group were [(1264.7 ±174.6)pg/mL, (0.58 ±0.07)], which were higher than those in control group [(2106.5 ±164.8)pg/mL,(0.49 ±0.08)](P<0.05).The total effective rate in observation group was 91.43%, which was higher than 71.43%in control group(P<0.05).Conclusion Atorvastatin joint neuroendocrine antagonist in treatment of chronic heart failure has clinical exact effect with no significant side effects.

Spontaneous rhythmic activity of pacemaker neurons in the central nervous system underlies fundamental neurological processes such as locomotion, cognition and circadian rhythm. Among the wide range of ion channels required for its generation, the Ca2+-activated K+(KCa) channels play a prominent role in maintaining physiologically-relevant frequency and pattern of pacemaker activity. Much of our understanding of the functions of KCa channels in pacemaker neurons have been derived from pharmacological studies using channel modulators, such as iberiotoxin and apamin. Despite the significant advances made, recent studies have painted an increasingly complex picture of the effects of widely used KCa channel modulators on unintended targets that may confound our under?standing of their functions. In this review, we discussed the utility and shortcomings of the KCa channel modulators, and highlighted the significance of these findings, because the KCa channel modulators have been used in early clinical trials to treat disorders ranging from Parkinson disease to alcoholism.

The preclinical safety assessment of hepatotoxicity drugs has a low sensitivity and low specificity. Related tests often generate false negative results and unexpected toxicity,which is one of the major reasons for the cessation of development and withdrawal from the market. Proteomics enjoys advantages of rapidness,high sensitivity and high throughout,and therefore can be used in the search for new biomarkers of hepatotoxicity in preclinical studies,leading to the development of safer drugs and a more efficient drug discovery process. In this review,the current preclinical biomark?ers of liver toxicity and development of proteomic technologies in the discovery and validation of bio?markers of drug-induced liver injury are described,in general the application of proteomics to Chinese medicine-induced liver toxicity in particular. Compared with traditional methods,proteomic technologies show promising results for the discovery of novel hepatotoxic markers. Proteomics,in conjugation with other omics techniques,will play a major role in the early stage of hepatotoxicity screening and will prove to be a good bridge in clinics in the future.

Objective To investigate the correlation between plasma fibronectin (Fn) level and the hemorrhagic transformation (HT) in patients with acute ischemic stroke.Methods Patients with acute ischemic stroke,symptom onset within 72 hours,no bleeding at baseline MRI (including gradient echo sequences) and without thrombolytic therapy were recruited prospectively.At 7 - 10 days after symptom onset reexamined MRI.The patients were divided into HT and non-HT groups according to whether they had a hemorrhage or not.The plasma Fn levels in both groups were compared.Logistic regression analysis was used to investigate the predictors of HT.Results A total of 78 patients were included,and among them 11 patients (14.10％ ) showed HT.The proportions of atrial fibrillation (36.4％ vs.9.0％,P =0.012) and cardioembolism (36.4％ vs.6.0％,P =0.021) in the HT group were significantly higher than those in the non-HT group.The baseline mean systolic blood pressure (182.09 ±20.73 mm Hg [ 1 mm Hg=0.133 kPa]vs.161.25 ±26.40)mm Hg,P =0.015),mean diastolic blood pressure (98.00 ± 11.26 mm Hg vs.86.60 ± 15.21 rnm Hg,P =0.020),and plasma Fn level (4 835.04 ± 756.30 μg/L vs.3 849.44 ± 1 289.18 μg/L,P =0.016) were all significantly higher than those in the non-HT group.Multivariate logistic regression analysis showed that plasma Fn level (odds ratio [ OR],1.001,95％ confidence interval [ CI] 1.000 - 1.001,P =0.017) and atrial fibrillation (OR,13.408,95％ CI 1.963 -91.592,P =0.008) were the independent predictors of HT in patients with acute ischemic stroke.Conclusions The plasma Fn level in patients with HT increased significantly.The plasma Fn level can be used as an independent predictor of non-thrombolytic HT after the onset of acute ischemic stroke.