Objective To analyze expression of angiopoietin-2 (Ang-2) protein in human esophageal squamous cell carcinoma (ESCC),and to investigate the relationship between Ang-2 protein and clinicopathologic factors.Methods 60 cases of ESCC,5 cases of latero-carcinoma tissues and 30 cases of normal tissue were immunohistochemically stained using Ang-2 monoclonal antibodies with PV-9000 non-biotin detection system.The positive expression rate and expression level of Ang-2 were evaluated.Results Ang-2 was mainly expressed in cytoplasm.The positive expression rates in normal epithelium,latero-carcinoma tissues and ESCC were respectively 3.33 ％ (1/30),40.00 ％ (2/5),53.33 ％ (32/60).Compared ESCC with normal epithelium and latero-carcinoma tissues,Ang-2 positive expression rate was significantly higher (P ＜ 0.05).Among ESCC,expression of Ang-2 was positively correlated with the tumor infiltration depth (rs =0.621,P =0.002),and inversely correlated with tumor differentiation status (rs =-0.309,P =0.018).Conclusions Ang-2 may play an important role in the carcinogenesis and development of ESCC.Tumor differentiation degree,infiltration depth and TNM staging are the independent prognostic indicators for ESCC patients.Combinative detecting the expression of Ang-2 may provide more accurate information in early diagnosis and predicting the prognosis of ESCC.

Imaging-guided radiofrequency ablation(RFA) is a safe and minimally-invasive interven-tional therapy with satisfactory short-term efficacy and less complications.Nowadays,RFA has been widely and successfully applied in the clinical practice for the treatment of hepatocellular carcinomas,lung cancers,adrenal malignancies,bone tumors and hypersplenism.For recent years,a combination of RFA with transcatheter arterial chemoembolization,chemical ablation or percutaneous vertebroplasty has become a new interventional therapeutic pattern for treating the tumors comprehensively,and it can be anticipated that by combining RFA with other therapeutic programs the effectiveness for tumors will be further improved.

Objective To investigate the role of various CA125 value in serum in instructing the treatment of endometrial cancer. Method The preoperative CA125 serum level of 147 cases of endometrisl cancer was examined by radioimmunoassay. Results Among patients with endometrial cancer, the CA125 level was higher in stages Ⅲ and Ⅳ than that in stages Ⅰ and Ⅱ (P<0.01). The sensitivity and specificity of CA125 > 20 kU/L in diagnosis of the endometrial cancer were 70.7% and 98.0%, and CA125 > 35 kU/L were 58.2% and 100.0%, respectively. The CA125 value of eases with cytological positive in abdomen was higher than that of cases with cytological negative. Conclusion The examination of CA125 serum level is valuable in the judgement of operative stages and lymph node metastasis in endometrial cancer.

Nowadays, lung cancer has become one of the most common malignant tumors. The animal models of lung cancer have played very important role in the research on the etiology, diagnosis and treatment of human lung cancers. According to the preparation method, the animal models of lung cancer can be divided into spontaneous, induced, transplanted and transgenic models. The transplanted animal models are most commonly used. Transplanted animal models can be classified into heterotopic transplantation model and orthotopic transplantation model, each has its own characteristics. This article aims to introduce the research progress of transplanted animal models of lung cancer in recent years, focusing on the establishment of animal model, the improvement of preparation of animal model, the application of imaging in vivo in evaluating the animal model, the use of transplanted animal model in the study of interventional therapy, etc.

ObjectiveTo explore change trend of Cardiac Troponin Ⅰ (cTnI) and Heart Fatty Acid-binding Protein(H-FABP) in serum during the perioperation of valve replacement.MethodsForty patients with heumatoid valvular heart disease were selected for this study,and the patients were randomly divided into two groups.Blood samples were taken from center vein,and the serum levels of cTnI and H-FABP were determined.The change of the serum levels of these two markers at different time points was recorded and compared between two groups.ResultsThere were significant differences in the concentration of cTnl in the cold crystalloid cardioplegia group ( F between group =2744.397,P ＜0.01 ; F interaction =125.345,P ＜0.01 ).There were significant differences in the concentration of cTnI in the cold blood cardioplegia group ( F between group =1056.357,P ＜ 0.01 ; Finteraction=64.242,P ＜ 0.01 ).There were significant differences in the concentration of H - FABP in the cold crystalloid cardioplegia group ( F between group =1775.022,P ＜0.01; F interaction =34.297,P ＜0.01 ).There were significant differences in the concentration of H -FABP in the cold blood cardioplegia group ( F between group =3064.451,P ＜0.01; Finteraction=60.472,P ＜O.01 ).ConclusionsH-FABP is suitable for early diagnosis of myocardial injury because of its myocardial specificity.There are positive correlation between the peak of H-FABP and myocardial injury.There are effective and short period of window for prediction of myocardial injury caused by H-FABP.H-FABP may be served as a good myocardial injury diagnostic markers.

Objective To understand the clinical features and laboratory examination and diagnosis of Langerhas cell histio-cytosis (LCH) in newborns. Methods Retrospective analysis of the clinical data of a neonatal case of LCH was performed. Results A one-day-old girl was admitted with chief complaint of eating less, less crying and skin rash for one day. The unequal sized rash was on the head, limbs, trunk, toe and oral mucosa. The skin rashes were protruding from the skin and were partially broken and crusted. Biopsy of the skin tissue showed the expression of S-100 and CD1α, consistent with the diagnosis of LCH. Conclusions When new-borns have special rash after birth, LCH should be considered. To avoid misdiagnosis, skin biopsy should be performed.

Objective To investigate features of the clinical and pathology of the 32 cases adolescent patients with carcinoma of esophagus.Methods Analysis of 32 cases with esophageal carcinoma by case -control study was carried out ,40 cases of up -50 years old patients,as control group.Results Compared with those in the control group,the adolescent patients rarely was found in early stage ,the pathological differentiation were worse,the more higher malignant degree,the more shorter survival time.Conclusion The clinical symptoms of adolescent patients with esophageal carcinoma is ocult, its early diagnosis rate is low ,and so the worse prognosis.

0 05). Lymphatic deposit and edema were not observed in follow-up cases. Conclusions Thoracic duct ligation could prevent the patients with esophageal carcinoma underwent resection from postoperative chylothorax. It can neither damage the cellular immunity nor lymphatic circulation.

Objective To investigate the correlation of Cathepsin D and P selectin expression with the clinico-pathologic features and prognosis of non-small cell lung cancer (NSCLC). Methods Immunohistochemistry (S-P method) was applied to detect the expression of Cathepsin D and P selection in 30 NSCLC specimens. Results The positive rates of cathepsin D and P selectin were 66 7% and 76 7% respectively. Cathepsin D expression in NSCLC was positive relation with the tumor size, TNM stage and lymph node metastasis(P0 05). The 3 years survival rate of the patients with the expression of both the Cathepsin D and P selectin was much lower than that of the patients with negative expression of both the Cathepsin D and P selectin(P

Objective To understand the epidemiological characteristics of HIV infection in voluntary blood donors in Liuzhou city to provide the basis for the blood donation recruit launched by the blood collection and tranfusion service agencies and reduce the risk of transfusion transmitted HIV.Methods The epidemiological investigation on the voluntary unpaid blood donors infected with HIV during 2008-2012 was performed for analyzing the infection characteristics.Results HIV infection in blood donors in Liuzhou city had no statistical difference between different years (P >0.05);the differences in different sexes,age groups,married and unmarried persons had statistically significant differences (P <0.01 );the education background less than middle school ac-counted for 52.94%;56.21% of the infectors were the sexual transmission.Conclusion HIV infection in voluntary unpaid blood donors in Liuzhou city is dominated by young adult men and the persons with the education level less than junior high school,and the heterosexual transmission is predominant.The donor recruitment work should pertinently exclude the persons of high-risk be-havior and collect blood in low risk population as far as possible.