Objective To evaluate the application of serological detection of Chlamydia pneumonia (Cpn) in patients with acute coronary syndrome (ACS) .Methods From Oct .2012 to Oct .2013 ,80 patients with ACS (ACS group) and 80 healthy subjects (control group) were enrolled and detected for Cpn DNA .According to the detected results ,ACS patients were divided to chlamydia group and non‐chlamydia group .Serum levels of tumor necrosis factor α(TNF‐α) ,C reaction protein (CRP) ,intercellular cell adhe‐sion molecule‐1 (ICAM‐1) and lipoprotein were also detected .Results In ACS group ,53 cases were Cpn positive and 27 cases were Cpn negative ,and in control group ,7 cases were Cpn positive and 83 cases were Cpn negative ,which were with statistical difference between the two groups (P<0 .05) .Serum levels of TNF‐α,CRP ,ICAM‐1 ,total cholesterol ,triacylglycerol and low density lipo‐protein cholesterol were higher in chlamydia group and that of high density lipoprotein cholesterol was lower than non‐chlamydia group (P<0 .05) .Conclusion Chlamydia pneumoniae infection might be correlated with ACS .Chlamydia pneumoniae infection could be involved in disease development by increasing levels of inflammatory cytokines in ACS patients ,and further improve serum lipid levels .

Objective To explore gait features at usual speed of community-dwelling elderly people,and its relationship with history of falls in the past one year.Methods According to history of falls in the past one year,135elderly people in Hefei City were recruited and divided into fallers(23 cases) and non-fallers(112 cases).Self-designed questionnaires were completed by all participants to collect data including gender,age,frequency of exercise,BMI,Barthel Index,MMSE,fear of fall and so on.Gait parameters were measured by IDEEA LifeGait when participants walked for 10 meters at usual speed.Results Between fallers with non-fallers,the time of Timed Up and Go Test had statistically significant differences(P＜0.05).Gait analysis results showed that swing power,foot fall,preswing angle,speed had statistically significant differences between two groups (all P＜0.05).Conclusion When community-dwelling elderly people are walking at usual speed,the shorter of the time of Timed Up and Go Test,the weaker of swing power,foot fall and pre-swing angle and the slower speed is,the more possible falls will occur.

Objective To compare optimal protein extraction method of velvet antler ( VA ) and explore the repairmen activity of velvet antler proteins ( VAPs ) on 1-methyl-4-phenylpyridinium ( MPP +) damaged nerve cell.Methods VAPs was obtained from fresh velvet antler by homogenization and micronization method respectively.The protein content of VAPs was measured by Bradford and the molecular weight was identified by SDS-PAGE.Scanning electron microscope (SEM) was perfomed to observe the ultrastructure of VAPs.Neuroblastoma cell line (SH-SY5Y) damage was induced by MPP +, and activity of each compound was compared by the proliferation of damaged cell detected by MTT method.Results The yield of micronization method of VAPs was higher than homogenization method and with better characters both in SDS-PAGE and SEM.Activity detection indicated that VAPs at the concentration of 125 μg/mL by homogenate method could significantly increase the proliferation rate of damaged SH-SY5Y cells.By contrast, VAPs by micronization method had more effective effect at 62.5μg/mL, and proliferation rate could reach 25.45% .Conclusion Micronization method does not only acquire more protein but also has cell proliferation activity, is a relatively ideal way of protein extraction of velvet antler.

Objective To evaluate the efficacy and superiority of intracardiac noncontact catheter mapping system for mapping and ablation of complicated arrhythmia.Methods 12 patients(9 males and 3 females,aged between 28 and 76 years) were included in this study.The characteristics of electrophysiological examination were as follows one was idiopathic ecotopic beats originated in right ventricular outflow tract(RVOT),two cases with idiopathic left ventricular tachycardia(ILVT),one atrial tachycardia(AT) of left atrium,two AT originated in right atrium,six atrial fibrillation(Afi) of left atrium.Among them,4 cases failed with conventional electrophysiological examination and radio frequency ablation(RFA),1 patient was reported recurrence.All patients were introduced 64 non-contact unipolar electrodes and ablation catheter to the same chamber via formal veins,reconstructed three-dimension geometry configuration,isopotential maps and analyzed the foci of reentrant activation and critical isthmus,then ablated the interested sites guided by noncontact mapping system.Results One idiopathic ectopic beat originated in RVOT near septum received segmental ablation.Two ILVT substrates located at left posterior-mid septum and left-posterior septum underwent segmental ablation.One AT of left atrium(LA) originated in right inferior pulmonary vein(RIPV) was given linear ablation between RIPV and mitral.One of the two AT from right atrium(RA) mapped the earliest activation in orifice of inferior cava vein and was given cricoid ablation;another AT originated in posterior wall of superior cava vein across crista terminal received linear lesion.One of the six Afi cases triggered by left atrial ectopic beats was given linear lesion between left superior pulmonary vein(LSPV) and right superior pulmonary vein(RSPV) during sinus rhythm;the other five cases mapped the reentrant origin site of Afi during persistent Afi rhythm received the catheter linear ablation,and the ablation lines respectively were created between RSPV and RIPV,LSPV and left inferior pulmonary vein(LIPV),LSPV and RSPV,LIPV and mitral isthmus.All patients succeeded and no obvious complication observed in operation and postoperation.None recurred during 2~26 month follow-up period.Conclusions It is safe and effective of intracardiac noncontact catheter mapping system for endocardium mapping and ablation of arrhythmia,especially for the clarity of electrophysiological mechanism of complicated arrhythmia and guiding RAF,which has potential clinical values.

Objective To report the clinical oberservation of 50 patients with implantable cardioverter defibrillator(ICD).Methods Observe the patients with ICD from May,1998 to Nov.2005.Results There were more than a thousand episodes of ventricular tachycardia ventricular fibrillation(VT/VF) detected and terminated by ICD devices.Conclusions ICD with tiered therapy function has high efficacy on the termination of ventricular tachyarrhythmias.It is important to follow up the patients and dynamically optimize the system of ICD.

Objective To observe the occurrence of the complication in patients under going interventional therapy for cardiovascular diseases and analyze its causes.Methods The data records of 1015 patients with interventional therapy for cardiovascular diseases in our hospital from October,2003 to October,2004(aged between 7 and 85 years,585 males and 430 females) were analyzed.Among them,520 patients received percutaneous transluminal coronary angioplasty and stent implantation,160 underwent radiofrequency catheter ablation,201 with permanent cardiac pacing,121 received blocking for congenital heart disease,10 received intraartery stent implantation and 29 with other interventional therapy.Results The complication occurred in 7.58% of 1015 patients.A higher incidence of hypotension and aberrant reflex has been reported(2.66% of all,mortality rate 0.39%).Conclusions Interventional therapy for cardiovascular diseases is an effective treatment with some risk.Serious pre-operational appraisal,enriching the experience of the doctors and strict post-operational monitoring may reduce the occurrence of the complication.

Objective To evaluate the effect of atrioventricular nodal ablation and permanent pacing(AVNAP) or atrioventricular nodal modification(AVNM) in the treatment of atrial fibrillation(AF).Methods 32 patients with drug refractory AF were divided into AVNAP group(18) and AVNM group(14).The patients were measured the left ventricular ejection fraction(LVEF) by the echocardiogram,tested by the 6 minu te walking test(6-MWT),assessed by NYHA function classification and the Mos 36~item short from health surrey(SF~36) before or after the treatments respectively.Results Mean follow up is 14 months.In AVNAP group,LVEF was increased from(47?9)% to(57?6)%(P

Objective To analyze 5 patients suptered heart failure and ventricular tachycardia((VT).)Methods The Implantable Biventricular pacing cardioverter defibrillator was used to analyzed 5 patients(4 with primary dilated cardiomyopathy and 1 with coronary heart disease) suffered from heart failure and ventricular tachycardia(VT).Results 2 cases had history of syncope,4 patients were implanted INSYNC 7272 implantable cardioverter defibrillator(ICD) and 1 patient was implanted V-350 ICD.During the 1~14 month follow-up period,all the cardio functions were improved without VT or syncope.1 patient with VT and VF was detected and terminated by ICD.The patient was saved.Conclusions Implantable biventricular pacing cardioverter defibrillator is an effective approach to treat sudden cardiac death,mortal ventricular arrhythmia and heart failure.

AIM: To investigate the relation between myocardial remodeling and the genesis of serum anti-?_3-adrenoceptor autoantibody,an animal model of heart failure(HF) was established and the biological effects of the autoantibody were observed.METHODS:(1) Healthy male Wistar rats were subjected to HF by constricting the abdominal aorta.(2) The anti-?_3-adrenoceptor autoantibody in the sera of HF rats was detected by ELISA with the synthetic peptide of the second extracellular loop of the ?_3-adrenoceptor used as the antigen.(3) IgG in the positive sera from HF rats was prepared using a MabTrap Kit(Amersham).(4) The effects of the autoantibody on the contractile response of adult(isolated) cardiomyocytes and on the beating rate of cultured neonatal rat cardiomyocytes were observed.RESULTS:(1) The positive rate of anti-?_3-adrenoceptor autoantibody of rats increased from 21.05% of pretreatment to 78.95% after heart failure(P

Objective To explore a more effective way to enhance the clinical efficacy of cluster immunotherapy for perennial allergic rhinitis. Methods A total of 60 perennial allergic rhinitis patients were evenly randomized into treatment goup and control group. The two groups were given cluster immunotherapy, and the treatment group was given daily vesiculating moxibustion additionally . Before and after treatment, the scores of traditional Chinese medical constitution and the rhinitis quality of life ( QOL) were observed, and one year after treatment, the therapeutic effect and safety were evaluated. Results ( 1) One year after treatment, the treatment group showed better total clinical efficiency than the control group ( 96.67% vs 80.00%, P<0.05). ( 2) After treatment, the average scores of Qi deficiency constitution, yang deficiency constitution and special constitution were significantly lower than those before treatment in the treatment group ( P<0.01). The improvement of Qi deficiency constitution and yang deficiency constitution in the treatment group was superior to that in the control group ( P<0.05). ( 3) The total QOL scores and the scores of each dimension of QOL scale were improved in both groups, and the differences were significant except for the emotion dimension ( P<0.01). Insignificant differences were shown between the two groups after treatment ( P>0.05). ( 4) Two cases had grade 1 general adverse reaction and 5 cases had local adverse reaction during cluster immunotherapy. During vesiculating moxibustion, 3 cases had blistering and the blistering disappeared after symptomatic treatment. Conclusion Daily vesiculating moxibustion combined with cluster immunity therapy is effective for the treatment of allergic rhinitis, showing good effect on improving Qi deficiency constitution, yang deficiency constitution and special constitution as well as the quality of life of the patients.