Medical imaging inspection technology is a practical major obligatory course for college students in medical imaging major.In order to overcome the defects of traditional teaching,we try to improve teaching methods,to carry out opening laboratory system,and to establish picture archiving and communication system ( PACS ) laboratory for the cultivation of applied talents of medical imaging.

Objective The predictive indexes which affect early neurological deterioration ( END) of patients with acute la-cunar stroke still remain unclear .The purpose of the article was to investigate the correlation between the location and numbers of cere -bral microbleeds (CMBs) and END in patients with first-onset lacunar stroke. Methods 217 patients with acute lacunar stroke hos-pitalized in Anhui Provincial Hospital within 24 hours after occurrence from Mar 2009 to May 2012 were prospectively enrolled .All pa-tients underwent susceptibility-weighted imaging MRI right after admission , followed by the investigation on the relationship of the loca-tion and numbers of CMBs and END . Results END occurred in 76 (35.0%) patients.Among these patients, there were 33 positive cases with CMBs (43.3%).There were no significant difference in END incidence between positive group and negative group (P=0.173).In the comparison of the numbers of CMBs foci , patients with more than 5 foci were more prone to END (P=0.005).Logistic regression analysis showed there was relation between CMBs foci numbers>5 and END (OR=3.14, 95% CI: 1.22 ~8.13), and there was no relation between CMBs foci numbers≤5 and END ( OR=1.12, 95%CI:0.53~2.37)as to CMBs negative patients.No signifi-cant relationship was found in CMBs location and END occurrence ( P>0.05). Conclusion The distribution of CMBs foci has nothing to do with early neurological fluctuation .However , more than 5 CMBs foci might be the independent prediction cause of END .

Objective To investigate the efficiency of ultrasound microbubble-mediated HGF gene transfer on liver fibrosis in rats. Methods The eukaryotic expression vector pMD18-T/HGF was constructed. The rats were randomly divided into 5 groups: normal group, model group, low dose group,middle dose group and high dose group. The rat liver fibrosis were induced. At day 14 after treatment, the rats were sacrificed, the ALT lever were evaluated, pathological evaluation of liver fibrosis were made,immunohistochemical evaluation of HGF protein expressions were observed, HGF mRNA expression were detected by RT-PCR. Results Liver 1function after the treatment showed that the treatment groups were better than model group ( P ＜ 0. 05). The difference between low dose group and normal group showed significant difference ( P ＜0.05), and the difference between middle/high dose groups and normal group showed no significant ( P ＞0. 05). Pathological results showed that the treatment groups could reduce liver fibrosis;treatment groups had the positive HGF expression by immunohistochemistry and the expression level increased with the increasement of the treatment dose ( P ＜0.05). RT-PCR showed HGF mRNA expression in the treatment groups were higher than in model group ( P ＜0.05). Conclusions Ultrasound microbubbles which carrying HGF can inhibit the process of hepatic fibrosis.

Objective To study the relationship between serum carbohydrate antigen 19-9 (CA19-9) and blood glucose level in patients with type 2 diabetes (T2DM).Methods Totally 784 T2DM patients and 197 healthy controls were enrolled in this study.Age,duration,body mass index (BMI),systolic blood pressure (SBP),diastolic blood pressure (DBP),levels of fasting blood glucose (FBG),postprandial blood glucose (PBG),hemoglobin A1c (HbA1c),total cholesterol (TC),triglyceride (TG),low-density lipoprotein cholesterol (LDL-C),high density lipoprotein cholesterol (HDL-C),blood uric acid,C peptide and CA 19-9 were recorded.Patients were divided into different groups according to the levels of HbA1 c and CA 19-9.The relationship between CA19-9 and influencing factors was analyzed.Results CA19-9 level was increased in T2DM patients compared with healthy controls [(22.08±14.94) U/mlvs.(10.24±6.31) U/ml,t=12.10,P＜0.001].The levels of SBP,DBP,FBG,PBG,C peptide,TC,TG,LDL and UA were higher and HDL C level was lower in T2DM patients than in healthy controls (all P＜0.05).The levels of FBG,PBG and CA19-9 were increased along with the increase of HbA1c level in T2MD patients with HbA1c level under 6.5％,T2MD patients with HbA1c level from 6.5％ to 7.5％,T2MD patients with HbA1c level over 7.5％ [FBG:(6.36±3.70) mmol/L,(9.01±4.01) mmol/L,(11.96±4.73) mmol/L;PBG:(9.69±3.21) mmol/L,(12.18±5.54) mmol/L,(15.27±5.08) mmol/L; CA19 9 (14.33±11.72) U/ml,(18.76±14.39) U/ml,(25.96±17.52) U/ml,respectively,all P＜0.05].The levels of FBG,HbA1c and pancreatic cancer incidence were higher in patients with elevated CA19-9 than with normal CA19-9 [(11.71±5.48)mmol/L vs.(9.78±4.38)mmol/L,(7.49±4.12)％ vs.(6.33±3.70)％,0％ vs.7.8％,t=-3.51,2.55,x2=83.58,allP＜0.05].Pearson correlation analysis showed that CA19-9 level was positively correlated with the levels of HbA 1 c and FBG in T2DM patients (r=0.281,0.134,both P＜0.05).Multiple Logistic regression analysis showed that the levels of FBG and HbA1c were correlated with the increase of CA19 9 in T2DM patients(OR=0.801,0.947,P=0.019,0.026).Conclusions Serum CA19-9 level is significantly increased in T2DM patients compared with the healthy people.Serum CA19-9 level is correlated with blood glucose control.

The 34 cases in the treatment group were treated with allicin injection through Murphy drip for a course of 10 days with nitroglycerin group as control. Results demonstrated that the total effective rate for symptoms and ECG were 82% and 62% respectively for the treatment group. It also markedly lowered the level of plasmal endothelin, and that of blood sugar in diabetics'. Adverst effects included local pain at the injection site and headache, the incidence of which were 18% and 6% respectively. This therapy is a satisfactory one for stubborn angina pectors.

Objective To investigate the impact of digital subtraction angiography(DSA)-guided neurointerventional thrombus removal combined with intravenous thrombolysis by tirofiban on the rate of recanalization of blood vessels,endothelial function,hemodynamics,and the degree of neurological deficit in patients with acute cerebral infarction.Methods Eighty patients with acute cerebral infarc-tion were selected as study subjects and randomly divided into observation group and control group u-sing the random number table method,with 40 patients in each group.The control group was treated with DSA-guided neurointerventional thrombus removal,and the observation group was treated with in-travenous thrombolysis with tirofiban on the basis of the control group.The rates of recanalization of blood vessels,endothelial function index levels,hemodynamic index levels,and the degree of neuro-logical deficit were compared between the two groups.Results The rates of recanalization of blood vessels in the observation group and control group were 90.00％(36/40)and 65.00％(26/40),re-spectively,with a significant difference(P＜0.05).After treatment,the serum endothelin-1(ET-1)levels in both groups were lower than those before treatment,and the nitric oxide(NO)levels were higher than those before treatment,and the ET-1 level in the observation group was lower than that in the control group,and the NO level was higher than that in the control group(P＜0.05).After treatment,the carotid extracranial resistance and characteristic impedance in both groups were lower than those before treatment,and the mean blood flow and mean blood flow velocity were higher than those before treatment,and the carotid extracranial resistance and characteristic impedance in the observation group were lower than those in the control group,and the mean blood flow and mean blood flow velocity were higher than those in the control group(P＜0.05).After treatment,the Na-tional Institutes of Health Stroke Scale(NIHSS)scores in both groups were lower than those before treatment,and the NIHSS score in the observation group was lower than that in the control group(P＜0.05).Conclusion DS A-guided neurointerventional thrombus removal combined with intra-venous thrombolysis by tirofiban can effectively increase the rate of recanalization of blood vessels in patients with acute cerebral infarction,improve endothelial function,alleviate neurological deficit symptoms,increase blood flow velocity and blood flow at ischemic lesion sites,and reduce carotid extracranial resistance and characteristic impedance.

Objective To investigate the impact of digital subtraction angiography(DSA)-guided neurointerventional thrombus removal combined with intravenous thrombolysis by tirofiban on the rate of recanalization of blood vessels,endothelial function,hemodynamics,and the degree of neurological deficit in patients with acute cerebral infarction.Methods Eighty patients with acute cerebral infarc-tion were selected as study subjects and randomly divided into observation group and control group u-sing the random number table method,with 40 patients in each group.The control group was treated with DSA-guided neurointerventional thrombus removal,and the observation group was treated with in-travenous thrombolysis with tirofiban on the basis of the control group.The rates of recanalization of blood vessels,endothelial function index levels,hemodynamic index levels,and the degree of neuro-logical deficit were compared between the two groups.Results The rates of recanalization of blood vessels in the observation group and control group were 90.00％(36/40)and 65.00％(26/40),re-spectively,with a significant difference(P＜0.05).After treatment,the serum endothelin-1(ET-1)levels in both groups were lower than those before treatment,and the nitric oxide(NO)levels were higher than those before treatment,and the ET-1 level in the observation group was lower than that in the control group,and the NO level was higher than that in the control group(P＜0.05).After treatment,the carotid extracranial resistance and characteristic impedance in both groups were lower than those before treatment,and the mean blood flow and mean blood flow velocity were higher than those before treatment,and the carotid extracranial resistance and characteristic impedance in the observation group were lower than those in the control group,and the mean blood flow and mean blood flow velocity were higher than those in the control group(P＜0.05).After treatment,the Na-tional Institutes of Health Stroke Scale(NIHSS)scores in both groups were lower than those before treatment,and the NIHSS score in the observation group was lower than that in the control group(P＜0.05).Conclusion DS A-guided neurointerventional thrombus removal combined with intra-venous thrombolysis by tirofiban can effectively increase the rate of recanalization of blood vessels in patients with acute cerebral infarction,improve endothelial function,alleviate neurological deficit symptoms,increase blood flow velocity and blood flow at ischemic lesion sites,and reduce carotid extracranial resistance and characteristic impedance.