Objective: To understand the use patterns, initial abuse age, initial abuse reasons and the demographic characteristic of opiates abusers at six areas in Hunan Province. Methods: Combined stratified cluster-sampling, household and cue investigation, 370 opiate abusers from 68 192 community residents aged between 15 and 50 years were interviewed. Results:About 95% opiate abuses reported that they had abused heroin in their life time, and the other had abused dolantin and/or methadone. Inhalation and Injection were the first two ways of administration (81.4% and 38.6%). The average initial abuse age was 27?6 years, 90.2% abuses reported that they had abused illicit drug before 35 years. Curiosity, peer pressures and exciting seeking were the first three main reasons of initial abuse (75.1%, 72.7% and 56.5%, respectively). Opiates abusers tended to be male (87.3%), young people aged below 35 years (71.1%), and 78.7% of the abusers got only junior school or below education. Subjects with unstable occupation and marital status had higher use rate than those with stable ones.Conclusion:Heroin was the major opiates abused, the main administration was inhalation and injection in Hunan Province. Young and middle aged male with low educational level or without stable occupation or marital status were the majority of opiates abusers.

Objective To study the clinical and pathological features of a case of retroperitoneal desmoplastic small round cell tumor. Methods One case of desmoplastic small round cell tumor was presented with review of the literature. Results A 11 years old child complained of abdominal pain and a 9.0 cm?6.0 cm?3.0 cm cystic tumor located in the left retroperitoneal space near the left kindey was resected.The connective tissue was significantly proliferated around the tumor cell nests. Immunohistochemical staining revealed CD 99 (+) and EWS/WT1 (+). After resection of the tumor,chemotherapy was performed with DDP?ifosfamide and VP16. No tumor recorrence has been noted 16 months after operation. Conclusions Desmoplastic small round cell tumor has specific clinicopathologic stigmata occuring usually in young males and the tumor was characterized by expression of MIC2 gene product CD 99 and the EWS/WT1 protein. Surgical resection with cheomotherapy is the treatment of choice.

0.05); VEGF at 50 ng/ml and 100 ng/ml with the exposure time of 5 d increased the incorporation( P

Objective To introduce a method of distal digital replantation with a venous drainage through medullary cavity.Methods Normal saline mixed with methylene b lue were perfused into digital arteries in 5fresh finger specimens and medullary cavities in 10fresh finger specimens.Then microanatomic al observation of vessels was performe d in the specimens.From April 1999to June 2000,15severed fingers in14cases were replanted with a venous drainage through medullary cavity.Medullary cavities of the distal and middle phalanx were drilled and made unobstructed after the distal joint of the finger was removed.The bones were fixed using only a single straig ht Kirschner wire in each finger.One or two digital arteries or the branch es of the terminal arteries were anasto mosed and two digital nerves sutured in each finger.Results Mi-croanatomical observation showed t hat a venous drainage through medullary cavity was dominating without su-perficial venous drainage and the di gital medullary cavity closely connected with the profunda digital vein s and the superficial digital veins by the venous system of the bone.All replan ted fingers survived in 14cases.The average postoperative follow-up wa s 7.8months,ranging from 6to 10mont hs.Nearly normal appearance and satisfactory sensation of the dista l phalanx were obtained with 2-PD of 5-9mm.Conclusion Distal digital replantation with a venous d rainage through medullary cavity is a new method.It has following advan-tages:simple and reliable operatio n,high survival rate and enlarged in dication of distal digital replanta tion.[

ObjectiveTo report 18 cases of Bowen dis ea se and erythroplasia of Queyrat.MethodsClinicopatholog ical data of 18 cases of Bowen disease and erythroplasia of Queyrat were present ed.10 cases (mean age 49 year)underwent circumcision and local resection and 8 c ases electric coagulation.Results15 cases have been fo llowed up for 4～48 months.2 cases had new disease at an untreated area and the o ther 13 have been free of the disease.Recurrence ocurred in 2 were cured with 5% 5-fluorouracil cream external application.ConclusionsThe result of the surgical treatment for Bowen disease and erythroplasia of Qu eyrat is good and an alternative therapy with 5-fluorouracil for external appli cation may be considered.Adequate biopsy including deeper tissue is necessary fo r differential diagnosis between carcinoma in situ and invasive penile carcinoma or a variety of lesions of the male genitalia.

OBJECTIVE To compare the effect and safety of coblation plasma surgery and beclomethasone dipropionate nasal spray in the treatment of perennial allergic rhinitis. METHODS A stratified,randomized,controlled trial of surgery and medicament was performed in the treatment of perennial allergic rhinitis. A total number of 102 patients were involved in the trial,including 56 cases with swelling of inferior turbinate,28 cases in surgery group,28 cases in medicament group; and 46 cases without swelling of inferior turbinate,23 cases in surgery group,23 cases in medicament group. Coblation plasma surgery was performed in all surgery groups. Each patient was given 50?4?2 ?g/d of beclomethasone dipropionate nasal spray in all medicament groups,Treatment time was 3 months. The symptom scores (SS)and visual analogue scale(VAS)were recorded. RESULTS Effect assessment showed that both methods had significant repressive effect on allergic symptoms,the effect of surgery group was better than that of medicament group at the 14th day,28th day and 3th month in cases with swelling of inferior turbinate. Difference between the surgery group and medicament group in cases without swelling of inferior turbinate had no statistic significance. CONCLUSION Both coblation plasma surgery and beclomethasone dipropionate nasal spray are effective to treat perennial allergic rhinitis. But coblation plasma surgery is better than medicament in cases with swelling of inferior turbinate.

Objective Use the automatic microbiological analysis system which for the streptococci (ATB rapid 32 Strep) to identify streptococci, and compare the examination speed and accuracy with the internationally recognized bacterium identification golden method API 20 Strep (streptococci identification biochemistry system) to enable the clinical microorganism room use ATB rapid 32 Strep identification system correctly, and provide the etiology basis to clinical promptly.Methods Isolate the bacterium from patient blood, cerebrospinal fluid, pig heart, pork and so on, then separate the streptococci in form to the blood plate, lay them in carbon dioxide incubator, after 35 ℃ 24 hours. Use ATB rapid 32 Strep,API 20 Strep and the traditional manual method to identify 18 strains of bacterias and carry on the medicine sensitive experiment.Results ATB rapid 32 Strep identified 16 strains as streptococcus suis II, 2 strains as streptococcus bovis II; API 20 Strep identified 17 strains as streptococcus suis II, 1 strain can not be identified; the tradition manually method identified all of the 18 strains as streptococcus group D . These 18 strains all sensitive to the Chloramphenicol , clindamycin, erythromycin, levofloxacin, penicillin, quinupristin/dalfopristin, cefotaxime, vancomycin and sulfafurazole , and resistant to the tetracyclin.Conclusion ATB rapid 32 Strep can identify the streptococcus suis in 4 hours , and the coincidence rate with API 20 Strep is 94.4%.So ATB rapid 32 Strep is one kind of system which could identify bacterium simply,quickly,accurately,and provide infectious disease etiology information for clinical immediately, satisfy the requirement of clinician and the microorganism examines.

Objective To describe well-child care guidelines in the United States and compare it to China's guidelines so as to inform child health policymakers and providers in developing improvements for China well-child care practices.Methods We used a multistage approach to identify features of well-child care guidelines.The following methods are used:literature review,key informant interviews,site visits to both China and the United States and summarization of experiences.The differences and similarities of the contents were compared.Results Both China and the United States emphasize the importance of well-child care and the basic contents such as immunization,growth monitoring,lab screening and health education are similar for well-child care from 0 to 6 years old.Differences exist in that China's well-child guidelines are a set of general principles,without specific instructions,and only cover children from 0 to 6 years old.There are no guidelines in China for children older than 6 years old.Conclusions China needs a more detailed well-child care guidelines with specific instructions for all-aged children; despite some similarities,there are applicable learnings from that of the United States' for improvement in China's.

Objective To investigate current situation of otoscope use by practitioners at community health-care service facilities in Hainan and Shenzhen and explore its importance and feasibility of spread of its use in community health-care service facilities in China.Methods Group discussion, telephone personal interview, otoscope market survey and literature research all were used among 112 practitioners at 67 community health-care service facilities in Hainan, 47 practitioners at 23 community health-care service facilities in Shenzhen, Guangdong, and 11 representatives involved in general practice training from 11 districts.Results In Hainan, only one community health-care service facility ( 1.5% ) was equipped with otoscopes and only one practitioner (0.9% ) could use it.In Shenzhen, two community health-care service facilities (8.7%) were equipped with it, but which had never be used.All the practitioners at community health-care service facilities surveyed have not been trained for its use, so most practitioners there did not have basic skills to use otoscope.Conclusions Otoscope is not equipped at most community health-care service facilities in Hainan and Shenzhen, and it is little used there even equipped with it.Otoscopy should be used as an adequate and feasible technique at community health-care service and be spread among general practitioners in China.

Objective: To study the effect of TIP30 gene on the cell cy cle distribution in adenoid cystic carcinoma ACC-M cells. Methods: Wild type TIP30 gene was transfected into ACC-M cells by using lipofectamin e, the postive cell clone was selected with G418,the expression of TIP30 protei n in the cells was detected by Western blot. Cell cycle distribution was detecte d by flow cytometry. Results:14 days after seletion anti-G418 c ell clone was obtained. Wstern blot revealed that the TIP30 protein was expresse d in the transfected cells.Flow cytometric analysis indicated that TIP30 induced a arrest of the cells in G 0-G 1 phase and led to a decrease of cell percent age in G 2-M and S phase. Conclusion:ACC-M can stably express exogene TIP30.TIP30 can inhibit proliferation of ACC-M cells.